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Understanding Hypothesis Tests: Significance Levels (Alpha) and P values in Statistics

Topics: Hypothesis Testing , Statistics

What do significance levels and P values mean in hypothesis tests? What is statistical significance anyway? In this post, I’ll continue to focus on concepts and graphs to help you gain a more intuitive understanding of how hypothesis tests work in statistics.

To bring it to life, I’ll add the significance level and P value to the graph in my previous post in order to perform a graphical version of the 1 sample t-test. It’s easier to understand when you can see what statistical significance truly means!

Here’s where we left off in my last post . We want to determine whether our sample mean (330.6) indicates that this year's average energy cost is significantly different from last year’s average energy cost of $260.

The probability distribution plot above shows the distribution of sample means we’d obtain under the assumption that the null hypothesis is true (population mean = 260) and we repeatedly drew a large number of random samples.

I left you with a question: where do we draw the line for statistical significance on the graph? Now we'll add in the significance level and the P value, which are the decision-making tools we'll need.

We'll use these tools to test the following hypotheses:

Null hypothesis: The population mean equals the hypothesized mean (260).
Alternative hypothesis: The population mean differs from the hypothesized mean (260).

What Is the Significance Level (Alpha)?

The significance level, also denoted as alpha or α, is the probability of rejecting the null hypothesis when it is true. For example, a significance level of 0.05 indicates a 5% risk of concluding that a difference exists when there is no actual difference.

These types of definitions can be hard to understand because of their technical nature. A picture makes the concepts much easier to comprehend!

The significance level determines how far out from the null hypothesis value we'll draw that line on the graph. To graph a significance level of 0.05, we need to shade the 5% of the distribution that is furthest away from the null hypothesis.

Probability plot that shows the critical regions for a significance level of 0.05

In the graph above, the two shaded areas are equidistant from the null hypothesis value and each area has a probability of 0.025, for a total of 0.05. In statistics, we call these shaded areas the critical region for a two-tailed test. If the population mean is 260, we’d expect to obtain a sample mean that falls in the critical region 5% of the time. The critical region defines how far away our sample statistic must be from the null hypothesis value before we can say it is unusual enough to reject the null hypothesis.

Our sample mean (330.6) falls within the critical region, which indicates it is statistically significant at the 0.05 level.

We can also see if it is statistically significant using the other common significance level of 0.01.

Probability plot that shows the critical regions for a significance level of 0.01

The two shaded areas each have a probability of 0.005, which adds up to a total probability of 0.01. This time our sample mean does not fall within the critical region and we fail to reject the null hypothesis. This comparison shows why you need to choose your significance level before you begin your study. It protects you from choosing a significance level because it conveniently gives you significant results!

Thanks to the graph, we were able to determine that our results are statistically significant at the 0.05 level without using a P value. However, when you use the numeric output produced by statistical software , you’ll need to compare the P value to your significance level to make this determination.

Ready for a demo of Minitab Statistical Software? Just ask!

What Are P values?

P-values are the probability of obtaining an effect at least as extreme as the one in your sample data, assuming the truth of the null hypothesis.

This definition of P values, while technically correct, is a bit convoluted. It’s easier to understand with a graph!

To graph the P value for our example data set, we need to determine the distance between the sample mean and the null hypothesis value (330.6 - 260 = 70.6). Next, we can graph the probability of obtaining a sample mean that is at least as extreme in both tails of the distribution (260 +/- 70.6).

Probability plot that shows the p-value for our sample mean

In the graph above, the two shaded areas each have a probability of 0.01556, for a total probability 0.03112. This probability represents the likelihood of obtaining a sample mean that is at least as extreme as our sample mean in both tails of the distribution if the population mean is 260. That’s our P value!

When a P value is less than or equal to the significance level, you reject the null hypothesis. If we take the P value for our example and compare it to the common significance levels, it matches the previous graphical results. The P value of 0.03112 is statistically significant at an alpha level of 0.05, but not at the 0.01 level.

If we stick to a significance level of 0.05, we can conclude that the average energy cost for the population is greater than 260.

A common mistake is to interpret the P-value as the probability that the null hypothesis is true. To understand why this interpretation is incorrect, please read my blog post How to Correctly Interpret P Values .

Discussion about Statistically Significant Results

A hypothesis test evaluates two mutually exclusive statements about a population to determine which statement is best supported by the sample data. A test result is statistically significant when the sample statistic is unusual enough relative to the null hypothesis that we can reject the null hypothesis for the entire population. “Unusual enough” in a hypothesis test is defined by:

The assumption that the null hypothesis is true—the graphs are centered on the null hypothesis value.
The significance level—how far out do we draw the line for the critical region?
Our sample statistic—does it fall in the critical region?

Keep in mind that there is no magic significance level that distinguishes between the studies that have a true effect and those that don’t with 100% accuracy. The common alpha values of 0.05 and 0.01 are simply based on tradition. For a significance level of 0.05, expect to obtain sample means in the critical region 5% of the time when the null hypothesis is true . In these cases, you won’t know that the null hypothesis is true but you’ll reject it because the sample mean falls in the critical region. That’s why the significance level is also referred to as an error rate!

This type of error doesn’t imply that the experimenter did anything wrong or require any other unusual explanation. The graphs show that when the null hypothesis is true, it is possible to obtain these unusual sample means for no reason other than random sampling error. It’s just luck of the draw.

Significance levels and P values are important tools that help you quantify and control this type of error in a hypothesis test. Using these tools to decide when to reject the null hypothesis increases your chance of making the correct decision.

If you like this post, you might want to read the other posts in this series that use the same graphical framework:

Previous: Why We Need to Use Hypothesis Tests
Next: Confidence Intervals and Confidence Levels

If you'd like to see how I made these graphs, please read: How to Create a Graphical Version of the 1-sample t-Test .

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over the shoulder view of a person in the lab with tubes in their hand. This represents statistical significance

What Is Statistical Significance & Why Learn It

08.04.2023 • 6 min read

Sarah Thomas

Subject Matter Expert

Learn what statistical significance means, why it is important, and how it’s calculated, and what the levels of significance mean.

In This Article

What Is Statistical Significance?

Examples of statistical significance, how is statistical significance calculated, what does the significance level mean.

A study claims people who practice intermittent fasting experience less severe complications from COVID-19, but how seriously should you take this claim? Statistical significance is a powerful tool that can help answer this question. It’s one of the main tools we have for assessing the validity of statistical findings.

In this article, we'll dive into what statistical significance is, how it's calculated, and why it's essential for making informed decisions based on data.

Statistical significance is a core concept in statistics. We use it in hypothesis testing to determine whether the results we obtain occurred by chance or whether they point to something relevant and true about the population we are studying.

Suppose you observe dogs whose owners spend more time with them at home live longer than those whose owners spend less time with them. The basic idea behind statistical significance is this: it helps you determine whether your observation is simply due to random variation in your samples or whether there’s strong enough evidence to support an underlying difference between dogs who spend more time with their owners and those who spend less.

If your results are “statistically significant,” your observation points to a meaningful difference rather than a difference observed by chance. If your results are not statistically significant, you do not have enough statistical evidence to rule out the possibility that what you observed is due to random variation in your samples. Maybe you observed longer life spans in one group of dogs simply because you happened to sample some ‌healthy dogs in one group or some less healthy ones in the other.

Statistical significance is crucial to ensure ‌our statistical findings are trustworthy and reliable, and we use it to avoid drawing false conclusions.

We use statistical analysis in many fields, so you’ll see mentions of statistical significance in many different contexts.

Here are some examples you might come across in the real world:

In finance, you may be interested in comparing the performance of different investment portfolios. If one portfolio performs better than another, you, as an investor, could use statistical tests to see if the higher returns are statistically significant. In other words, you could test to see whether the higher returns were likely the result of random variation or whether some other reason explains why one portfolio performed better. 

In medical research, you may be interested in comparing the outcomes of patients in a treatment group to those in a control group. The patients in the treatment group receive a newly developed drug to manage a disease, while patients in the treatment group do not receive the new treatment.

At the end of your study, you compare the health outcomes of each group. If you observe that the patients in the treatment group had better outcomes, you will want to conduct a hypothesis test to see if the difference in outcomes is statistically significant—i.e., due to the difference in treatment.

Agriculture

Agronomists use hypothesis tests to determine what soil conditions and management types lead to the best crop yields. Like a medical researcher who compares treatment and control groups, agronomists conduct hypothesis tests to see if there are statistically significant differences between crops grown under different conditions.

In business, there are many applications of statistical testing. For example, if you hypothesize that a product tweak led to greater customer retention, you could use a statistical test to see if your results are statistically significant.

To determine statistical significance, you first need to articulate the main elements of your hypothesis test. You’ll need to articulate a null hypothesis and an alternative hypothesis , and you’ll need to choose a significance level for your test.

The null hypothesis represents the assumption that there's no significant difference between the variables you are studying. For example, a new drug has no effect on patient outcomes.

The alternative hypothesis represents what you suspect might be true. For example, a new drug improves patient outcomes. The null and alternative hypotheses must be mutually exclusive statements, meaning that the null hypothesis must be false for the alternative hypothesis to be true.

The significance level of a hypothesis test is the threshold you use to determine whether or not your results are statistically significant. The significance level is the probability of falsely rejecting the null hypothesis when it is actually true.

The most commonly used significance levels are:

0.10 (or 10%)

0.05 (or 5%)

Once you’ve stated your hypotheses and significance level, you’ll collect data and calculate a test statistic from your data. You then need to calculate a p-value for your test statistic. A p-value represents the probability of observing a result as extreme or more extreme than the one you obtained, assuming that the null hypothesis is true.

If the p-value is smaller than your significance level, you reject the null hypothesis in favor of the alternative hypothesis. The smaller your p-value is, the more conviction you can have in rejecting the null hypothesis. If the p-value exceeds the significance level, you fail to reject the null hypothesis.

graph showing steps in a hypothesis test

The significance level—or alpha level (α)—is a benchmark we use in statistical hypothesis testing. It is the probability threshold below which you reject the null hypothesis.

The most commonly used significance level is 0.05 (or 5%), but you can choose a lower or higher significance level in your testing depending on your priorities. With a smaller alpha, you have a smaller chance of making a Type I error in your analysis.

A Type I error is when you accidentally reject the null hypothesis when, in fact, the null hypothesis is true. On the other hand, the smaller your significance level is, the more likely you are to make a Type II error. A Type II error is when you fail to reject a false null hypothesis.

A significance level of 0.05 means if you calculate a p-value less than 0.05, you will reject the null hypothesis in favor of the alternative hypothesis. It also means you will have a 5% chance of rejecting the null hypothesis when it is actually true (i.e., you will have a 5% chance of making a Type I error).

Is statistical significance the only way to assess the validity of a statistical study?

Statistical significance is one of many things you should check for when assessing a statistical study. A statistically significant result is not enough to guarantee the validity of a statistical result. Several other factors affect the reliability of research findings, such as sample size, the type of data used, study design, and the presence of confounding variables.

You always need to use statistical significance as a tool in combination with other methods to ensure the quality of your research.

Is statistical significance the same thing as effect size?

Statistical significance and effect size are not the same thing. Statistical significance assesses whether our findings are due to random chance. Effect size measures the magnitude of the difference between two groups or the strength of the relationship between two variables.

Just because your data analysis returns statistically significant results, it does mean that you have found a large effect size. For example, you may find a statistically significant result that dogs who spend more time with their owners live longer, but you might only find a small effect.

Effect size is sometimes called practical significance because statistically significant results are only “practically significant” when the results are large enough for us to care about.

What is the difference between statistical significance and statistical power?

Statistical power is the power of a statistical test to correctly reject the null hypothesis when a given alternative hypothesis is true. It is the ability to detect an effect in a sample data set when the effect is, in fact, present in the population. Statistical significance is one of the factors that impacts power.

A higher significance level, having a larger sample, a larger effect size, and lower variability within the population, are all elements that increase statistical power.

What is the relationship between significance levels and confidence levels?

The significance (or alpha) level is the threshold we use to decide whether or not to reject the null hypothesis. Confidence intervals, on the other hand, are a range of values that contain the true population parameter. For example, a 95% confidence interval means 95% of the intervals would contain the true population parameter if a study were repeated many times.

Statistical significance and confidence levels are both related to the probability of making a Type I error. A Type I error—sometimes called a false-positive—is an error that occurs when a statistician rejects the null hypothesis when it is actually true. The probability of making a Type I error is equal to the significance level and is also the complement of the confidence level.

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StatPearls [Internet].

Hypothesis testing, p values, confidence intervals, and significance.

Jacob Shreffler ; Martin R. Huecker .

Affiliations

Last Update: March 13, 2023 .

Definition/Introduction

Medical providers often rely on evidence-based medicine to guide decision-making in practice. Often a research hypothesis is tested with results provided, typically with p values, confidence intervals, or both. Additionally, statistical or research significance is estimated or determined by the investigators. Unfortunately, healthcare providers may have different comfort levels in interpreting these findings, which may affect the adequate application of the data.

Issues of Concern

Without a foundational understanding of hypothesis testing, p values, confidence intervals, and the difference between statistical and clinical significance, it may affect healthcare providers' ability to make clinical decisions without relying purely on the research investigators deemed level of significance. Therefore, an overview of these concepts is provided to allow medical professionals to use their expertise to determine if results are reported sufficiently and if the study outcomes are clinically appropriate to be applied in healthcare practice.

Hypothesis Testing

Investigators conducting studies need research questions and hypotheses to guide analyses. Starting with broad research questions (RQs), investigators then identify a gap in current clinical practice or research. Any research problem or statement is grounded in a better understanding of relationships between two or more variables. For this article, we will use the following research question example:

Research Question: Is Drug 23 an effective treatment for Disease A?

Research questions do not directly imply specific guesses or predictions; we must formulate research hypotheses. A hypothesis is a predetermined declaration regarding the research question in which the investigator(s) makes a precise, educated guess about a study outcome. This is sometimes called the alternative hypothesis and ultimately allows the researcher to take a stance based on experience or insight from medical literature. An example of a hypothesis is below.

Research Hypothesis: Drug 23 will significantly reduce symptoms associated with Disease A compared to Drug 22.

The null hypothesis states that there is no statistical difference between groups based on the stated research hypothesis.

Researchers should be aware of journal recommendations when considering how to report p values, and manuscripts should remain internally consistent.

Regarding p values, as the number of individuals enrolled in a study (the sample size) increases, the likelihood of finding a statistically significant effect increases. With very large sample sizes, the p-value can be very low significant differences in the reduction of symptoms for Disease A between Drug 23 and Drug 22. The null hypothesis is deemed true until a study presents significant data to support rejecting the null hypothesis. Based on the results, the investigators will either reject the null hypothesis (if they found significant differences or associations) or fail to reject the null hypothesis (they could not provide proof that there were significant differences or associations).

To test a hypothesis, researchers obtain data on a representative sample to determine whether to reject or fail to reject a null hypothesis. In most research studies, it is not feasible to obtain data for an entire population. Using a sampling procedure allows for statistical inference, though this involves a certain possibility of error. [1] When determining whether to reject or fail to reject the null hypothesis, mistakes can be made: Type I and Type II errors. Though it is impossible to ensure that these errors have not occurred, researchers should limit the possibilities of these faults. [2]

Significance

Significance is a term to describe the substantive importance of medical research. Statistical significance is the likelihood of results due to chance. [3] Healthcare providers should always delineate statistical significance from clinical significance, a common error when reviewing biomedical research. [4] When conceptualizing findings reported as either significant or not significant, healthcare providers should not simply accept researchers' results or conclusions without considering the clinical significance. Healthcare professionals should consider the clinical importance of findings and understand both p values and confidence intervals so they do not have to rely on the researchers to determine the level of significance. [5] One criterion often used to determine statistical significance is the utilization of p values.

P values are used in research to determine whether the sample estimate is significantly different from a hypothesized value. The p-value is the probability that the observed effect within the study would have occurred by chance if, in reality, there was no true effect. Conventionally, data yielding a p<0.05 or p<0.01 is considered statistically significant. While some have debated that the 0.05 level should be lowered, it is still universally practiced. [6] Hypothesis testing allows us to determine the size of the effect.

An example of findings reported with p values are below:

Statement: Drug 23 reduced patients' symptoms compared to Drug 22. Patients who received Drug 23 (n=100) were 2.1 times less likely than patients who received Drug 22 (n = 100) to experience symptoms of Disease A, p<0.05.

Statement:Individuals who were prescribed Drug 23 experienced fewer symptoms (M = 1.3, SD = 0.7) compared to individuals who were prescribed Drug 22 (M = 5.3, SD = 1.9). This finding was statistically significant, p= 0.02.

For either statement, if the threshold had been set at 0.05, the null hypothesis (that there was no relationship) should be rejected, and we should conclude significant differences. Noticeably, as can be seen in the two statements above, some researchers will report findings with < or > and others will provide an exact p-value (0.000001) but never zero [6] . When examining research, readers should understand how p values are reported. The best practice is to report all p values for all variables within a study design, rather than only providing p values for variables with significant findings. [7] The inclusion of all p values provides evidence for study validity and limits suspicion for selective reporting/data mining.

While researchers have historically used p values, experts who find p values problematic encourage the use of confidence intervals. [8] . P-values alone do not allow us to understand the size or the extent of the differences or associations. [3] In March 2016, the American Statistical Association (ASA) released a statement on p values, noting that scientific decision-making and conclusions should not be based on a fixed p-value threshold (e.g., 0.05). They recommend focusing on the significance of results in the context of study design, quality of measurements, and validity of data. Ultimately, the ASA statement noted that in isolation, a p-value does not provide strong evidence. [9]

When conceptualizing clinical work, healthcare professionals should consider p values with a concurrent appraisal study design validity. For example, a p-value from a double-blinded randomized clinical trial (designed to minimize bias) should be weighted higher than one from a retrospective observational study [7] . The p-value debate has smoldered since the 1950s [10] , and replacement with confidence intervals has been suggested since the 1980s. [11]

Confidence Intervals

A confidence interval provides a range of values within given confidence (e.g., 95%), including the accurate value of the statistical constraint within a targeted population. [12] Most research uses a 95% CI, but investigators can set any level (e.g., 90% CI, 99% CI). [13] A CI provides a range with the lower bound and upper bound limits of a difference or association that would be plausible for a population. [14] Therefore, a CI of 95% indicates that if a study were to be carried out 100 times, the range would contain the true value in 95, [15] confidence intervals provide more evidence regarding the precision of an estimate compared to p-values. [6]

In consideration of the similar research example provided above, one could make the following statement with 95% CI:

Statement: Individuals who were prescribed Drug 23 had no symptoms after three days, which was significantly faster than those prescribed Drug 22; there was a mean difference between the two groups of days to the recovery of 4.2 days (95% CI: 1.9 – 7.8).

It is important to note that the width of the CI is affected by the standard error and the sample size; reducing a study sample number will result in less precision of the CI (increase the width). [14] A larger width indicates a smaller sample size or a larger variability. [16] A researcher would want to increase the precision of the CI. For example, a 95% CI of 1.43 – 1.47 is much more precise than the one provided in the example above. In research and clinical practice, CIs provide valuable information on whether the interval includes or excludes any clinically significant values. [14]

Null values are sometimes used for differences with CI (zero for differential comparisons and 1 for ratios). However, CIs provide more information than that. [15] Consider this example: A hospital implements a new protocol that reduced wait time for patients in the emergency department by an average of 25 minutes (95% CI: -2.5 – 41 minutes). Because the range crosses zero, implementing this protocol in different populations could result in longer wait times; however, the range is much higher on the positive side. Thus, while the p-value used to detect statistical significance for this may result in "not significant" findings, individuals should examine this range, consider the study design, and weigh whether or not it is still worth piloting in their workplace.

Similarly to p-values, 95% CIs cannot control for researchers' errors (e.g., study bias or improper data analysis). [14] In consideration of whether to report p-values or CIs, researchers should examine journal preferences. When in doubt, reporting both may be beneficial. [13] An example is below:

Reporting both: Individuals who were prescribed Drug 23 had no symptoms after three days, which was significantly faster than those prescribed Drug 22, p = 0.009. There was a mean difference between the two groups of days to the recovery of 4.2 days (95% CI: 1.9 – 7.8).

Clinical Significance

Recall that clinical significance and statistical significance are two different concepts. Healthcare providers should remember that a study with statistically significant differences and large sample size may be of no interest to clinicians, whereas a study with smaller sample size and statistically non-significant results could impact clinical practice. [14] Additionally, as previously mentioned, a non-significant finding may reflect the study design itself rather than relationships between variables.

Healthcare providers using evidence-based medicine to inform practice should use clinical judgment to determine the practical importance of studies through careful evaluation of the design, sample size, power, likelihood of type I and type II errors, data analysis, and reporting of statistical findings (p values, 95% CI or both). [4] Interestingly, some experts have called for "statistically significant" or "not significant" to be excluded from work as statistical significance never has and will never be equivalent to clinical significance. [17]

The decision on what is clinically significant can be challenging, depending on the providers' experience and especially the severity of the disease. Providers should use their knowledge and experiences to determine the meaningfulness of study results and make inferences based not only on significant or insignificant results by researchers but through their understanding of study limitations and practical implications.

Nursing, Allied Health, and Interprofessional Team Interventions

All physicians, nurses, pharmacists, and other healthcare professionals should strive to understand the concepts in this chapter. These individuals should maintain the ability to review and incorporate new literature for evidence-based and safe care.

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Disclosure: Jacob Shreffler declares no relevant financial relationships with ineligible companies.

Disclosure: Martin Huecker declares no relevant financial relationships with ineligible companies.

This book is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ), which permits others to distribute the work, provided that the article is not altered or used commercially. You are not required to obtain permission to distribute this article, provided that you credit the author and journal.

Cite this Page Shreffler J, Huecker MR. Hypothesis Testing, P Values, Confidence Intervals, and Significance. [Updated 2023 Mar 13]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-.

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Hypothesis Testing (cont...)

Hypothesis testing, the null and alternative hypothesis.

In order to undertake hypothesis testing you need to express your research hypothesis as a null and alternative hypothesis. The null hypothesis and alternative hypothesis are statements regarding the differences or effects that occur in the population. You will use your sample to test which statement (i.e., the null hypothesis or alternative hypothesis) is most likely (although technically, you test the evidence against the null hypothesis). So, with respect to our teaching example, the null and alternative hypothesis will reflect statements about all statistics students on graduate management courses.

The null hypothesis is essentially the "devil's advocate" position. That is, it assumes that whatever you are trying to prove did not happen ( hint: it usually states that something equals zero). For example, the two different teaching methods did not result in different exam performances (i.e., zero difference). Another example might be that there is no relationship between anxiety and athletic performance (i.e., the slope is zero). The alternative hypothesis states the opposite and is usually the hypothesis you are trying to prove (e.g., the two different teaching methods did result in different exam performances). Initially, you can state these hypotheses in more general terms (e.g., using terms like "effect", "relationship", etc.), as shown below for the teaching methods example:

Null Hypotheses (H ):	Undertaking seminar classes has no effect on students' performance.
Alternative Hypothesis (H ):	Undertaking seminar class has a positive effect on students' performance.

Depending on how you want to "summarize" the exam performances will determine how you might want to write a more specific null and alternative hypothesis. For example, you could compare the mean exam performance of each group (i.e., the "seminar" group and the "lectures-only" group). This is what we will demonstrate here, but other options include comparing the distributions , medians , amongst other things. As such, we can state:

Null Hypotheses (H ):	The mean exam mark for the "seminar" and "lecture-only" teaching methods is the same in the population.
Alternative Hypothesis (H ):	The mean exam mark for the "seminar" and "lecture-only" teaching methods is not the same in the population.

Now that you have identified the null and alternative hypotheses, you need to find evidence and develop a strategy for declaring your "support" for either the null or alternative hypothesis. We can do this using some statistical theory and some arbitrary cut-off points. Both these issues are dealt with next.

Significance levels

The level of statistical significance is often expressed as the so-called p -value . Depending on the statistical test you have chosen, you will calculate a probability (i.e., the p -value) of observing your sample results (or more extreme) given that the null hypothesis is true . Another way of phrasing this is to consider the probability that a difference in a mean score (or other statistic) could have arisen based on the assumption that there really is no difference. Let us consider this statement with respect to our example where we are interested in the difference in mean exam performance between two different teaching methods. If there really is no difference between the two teaching methods in the population (i.e., given that the null hypothesis is true), how likely would it be to see a difference in the mean exam performance between the two teaching methods as large as (or larger than) that which has been observed in your sample?

So, you might get a p -value such as 0.03 (i.e., p = .03). This means that there is a 3% chance of finding a difference as large as (or larger than) the one in your study given that the null hypothesis is true. However, you want to know whether this is "statistically significant". Typically, if there was a 5% or less chance (5 times in 100 or less) that the difference in the mean exam performance between the two teaching methods (or whatever statistic you are using) is as different as observed given the null hypothesis is true, you would reject the null hypothesis and accept the alternative hypothesis. Alternately, if the chance was greater than 5% (5 times in 100 or more), you would fail to reject the null hypothesis and would not accept the alternative hypothesis. As such, in this example where p = .03, we would reject the null hypothesis and accept the alternative hypothesis. We reject it because at a significance level of 0.03 (i.e., less than a 5% chance), the result we obtained could happen too frequently for us to be confident that it was the two teaching methods that had an effect on exam performance.

Whilst there is relatively little justification why a significance level of 0.05 is used rather than 0.01 or 0.10, for example, it is widely used in academic research. However, if you want to be particularly confident in your results, you can set a more stringent level of 0.01 (a 1% chance or less; 1 in 100 chance or less).

One- and two-tailed predictions

When considering whether we reject the null hypothesis and accept the alternative hypothesis, we need to consider the direction of the alternative hypothesis statement. For example, the alternative hypothesis that was stated earlier is:

Alternative Hypothesis (H ):

Undertaking seminar classes has a positive effect on students' performance.

The alternative hypothesis tells us two things. First, what predictions did we make about the effect of the independent variable(s) on the dependent variable(s)? Second, what was the predicted direction of this effect? Let's use our example to highlight these two points.

Sarah predicted that her teaching method (independent variable: teaching method), whereby she not only required her students to attend lectures, but also seminars, would have a positive effect (that is, increased) students' performance (dependent variable: exam marks). If an alternative hypothesis has a direction (and this is how you want to test it), the hypothesis is one-tailed. That is, it predicts direction of the effect. If the alternative hypothesis has stated that the effect was expected to be negative, this is also a one-tailed hypothesis.

Alternatively, a two-tailed prediction means that we do not make a choice over the direction that the effect of the experiment takes. Rather, it simply implies that the effect could be negative or positive. If Sarah had made a two-tailed prediction, the alternative hypothesis might have been:

Alternative Hypothesis (H ):

Undertaking seminar classes has an effect on students' performance.

In other words, we simply take out the word "positive", which implies the direction of our effect. In our example, making a two-tailed prediction may seem strange. After all, it would be logical to expect that "extra" tuition (going to seminar classes as well as lectures) would either have a positive effect on students' performance or no effect at all, but certainly not a negative effect. However, this is just our opinion (and hope) and certainly does not mean that we will get the effect we expect. Generally speaking, making a one-tail prediction (i.e., and testing for it this way) is frowned upon as it usually reflects the hope of a researcher rather than any certainty that it will happen. Notable exceptions to this rule are when there is only one possible way in which a change could occur. This can happen, for example, when biological activity/presence in measured. That is, a protein might be "dormant" and the stimulus you are using can only possibly "wake it up" (i.e., it cannot possibly reduce the activity of a "dormant" protein). In addition, for some statistical tests, one-tailed tests are not possible.

Rejecting or failing to reject the null hypothesis

Let's return finally to the question of whether we reject or fail to reject the null hypothesis.

If our statistical analysis shows that the significance level is below the cut-off value we have set (e.g., either 0.05 or 0.01), we reject the null hypothesis and accept the alternative hypothesis. Alternatively, if the significance level is above the cut-off value, we fail to reject the null hypothesis and cannot accept the alternative hypothesis. You should note that you cannot accept the null hypothesis, but only find evidence against it.

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Level of Significance & Hypothesis Testing

level of significance and hypothesis testing

In hypothesis testing , the level of significance is a measure of how confident you can be about rejecting the null hypothesis. This blog post will explore what hypothesis testing is and why understanding significance levels are important for your data science projects. In addition, you will also get to test your knowledge of level of significance towards the end of the blog with the help of quiz . These questions can help you test your understanding and prepare for data science / statistics interviews . Before we look into what level of significance is, let’s quickly understand what is hypothesis testing.

Table of Contents

What is Hypothesis testing and how is it related to significance level?

Hypothesis testing can be defined as tests performed to evaluate whether a claim or theory about something is true or otherwise. In order to perform hypothesis tests, the following steps need to be taken:

Hypothesis formulation: Formulate the null and alternate hypothesis
Data collection: Gather the sample of data
Statistical tests: Determine the statistical test and test statistics. The statistical tests can be z-test or t-test depending upon the number of data samples and/or whether the population variance is known otherwise.
Set the level of significance
Calculate the p-value
Draw conclusions: Based on the value of p-value and significance level, reject the null hypothesis or otherwise.

A detailed explanation is provided in one of my related posts titled hypothesis testing explained with examples .

What is the level of significance?

The level of significance is defined as the criteria or threshold value based on which one can reject the null hypothesis or fail to reject the null hypothesis. The level of significance determines whether the outcome of hypothesis testing is statistically significant or otherwise. The significance level is also called as alpha level.

Another way of looking at the level of significance is the value which represents the likelihood of making a type I error . You may recall that Type I error occurs while evaluating hypothesis testing outcomes. If you reject the null hypothesis by mistake, you end up making a Type I error. This scenario is also termed as “false positive”. Take an example of a person alleged with committing a crime. The null hypothesis is that the person is not guilty. Type I error happens when you reject the null hypothesis by mistake. Given the example, a Type I error happens when you reject the null hypothesis that the person is not guilty by mistake. The innocent person is convicted.

The level of significance can take values such as 0.1 , 0.05 , 0.01 . The most common value of the level of significance is 0.05 . The lower the value of significance level, the lesser is the chance of type I error. That would essentially mean that the experiment or hypothesis testing outcome would really need to be highly precise for one to reject the null hypothesis. The likelihood of making a type I error would be very low. However, that does increase the chances of making type II errors as you may make mistakes in failing to reject the null hypothesis. You may want to read more details in relation to type I errors and type II errors in this post – Type I errors and Type II errors in hypothesis testing

The outcome of the hypothesis testing is evaluated with the help of a p-value. If the p-value is less than the level of significance, then the hypothesis testing outcome is statistically significant. On the other hand, if the hypothesis testing outcome is not statistically significant or the p-value is more than the level of significance, then we fail to reject the null hypothesis. The same is represented in the picture below for a right-tailed test. I will be posting details on different types of tail test in future posts.

The picture below represents the concept for two-tailed hypothesis test:

level of significance and two-tailed test

For example: Let’s say that a school principal wants to find out whether extra coaching of 2 hours after school help students do better in their exams. The hypothesis would be as follows:

Null hypothesis : There is no difference between the performance of students even after providing extra coaching of 2 hours after the schools are over.
Alternate hypothesis : Students perform better when they get extra coaching of 2 hours after the schools are over. This hypothesis testing example would require a level of significant value at 0.05 or simply put, it would need to be highly precise that there’s actually a difference between the performance of students based on whether they take extra coaching.

Now, let’s say that we conduct this experiment with 100 students and measure their scores in exams. The test statistics is computed to be z=-0.50 (p-value=0.62). Since the p-value is more than 0.05, we fail to reject the null hypothesis. There is not enough evidence to show that there’s a difference in the performance of students based on whether they get extra coaching.

While performing hypothesis tests or experiments, it is important to keep the level of significance in mind.

Why does one need a level of significance?

In hypothesis tests, if we do not have some sort of threshold by which to determine whether your results are statistically significant enough for you to reject the null hypothesis, then it would be tough for us to determine whether your findings are significant or not. This is why we take into account levels of significance when performing hypothesis tests and experiments.

Since hypothesis testing helps us in making decisions about our data, having a level of significance set up allows one to know what sort of chances their findings might have of actually being due to the null hypothesis. If you set your level of significance at 0.05 for example, it would mean that there’s only a five percent chance that the difference between groups (assuming two groups are tested) is due to random sampling error. So if we found a difference in the performance of students based on whether they take extra coaching, we would need to consider other factors that could have contributed to the difference.

This is why hypothesis testing and level of significance go hand in hand with one another: hypothesis tests help us know whether our data falls within a certain range where it’s statistically significant or not so statistically significant whereas the level of significance tells us how likely is it that our hypothesis testing results are not due to random sampling error.

How is the level of significance used in hypothesis testing?

The level of significance along with the test statistic and p-value formed a key part of hypothesis testing. The value that you derive from hypothesis testing depends on whether or not you accept/reject the null hypothesis, given your findings at each step. Before going into rejection vs non-rejection, let’s understand the terms better.

If the test statistic falls within the critical region, you reject the null hypothesis. This means that your findings are statistically significant and support the alternate hypothesis. The value of the p-value determines how likely it is for finding this outcome if, in fact, the null hypothesis were true. If the p-value is less than or equal to the level of significance, you reject the null hypothesis. This means that your hypothesis testing outcome was statistically significant at a certain degree and in favor of the alternate hypothesis.

If on the other hand, the p-value is greater than alpha level or significance level, then you fail to reject the null hypothesis. These findings are not statistically significant enough for one to reject the null hypothesis. The same is represented in the diagram below:

Level of Significance – Quiz / Interview Questions

Here are some practice questions which can help you in testing your questions, and, also prepare for interviews.

#1. Which one of the following is considered most popular choice of significance level?

#2. the p-value of 0.03 is statistically significant for significance level as 0.01, #3. the p-value less than the level of significance would mean which of the following, #4. which of the following is looks to be inappropriate level of significance, #5. which of the following will result in greater type ii error, #6. the statistically significant outcome of hypothesis testing would mean which of the following, #7. level of significance is also called as ________, #8. which of the following will result in greater type i error, recent posts.

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Oops! Check your answers again. The minimum pass percentage is 70%.

Hypothesis testing is an important statistical concept that helps us determine whether the claim made about anything is true or otherwise. The hypothesis test statistic, level of significance, and p-value all work together to help you make decisions about your data. If our hypothesis tests show enough evidence to reject the null hypothesis, then we know statistically significant findings are at hand. This post gave you ideas for how you can use hypothesis testing in your experiments by understanding what it means when someone rejects or fails to reject the null hypothesis.

Ajitesh Kumar

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What Does It Mean for Research to Be Statistically Significant?

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What does it mean to be statistically significant, an example of null hypothesis significance testing, measuring statistical significance: understanding the p value (significance level), what factors affect the power of hypothesis test, 1. sample size, 2. significance level, 3. standard deviations, 4. effect size, why is statistical significance important for researchers, does your study need to be statistically significant, practical significance vs. statistical significance, part 1: how is statistical significance defined in research.

The world today is drowning in data.

That may sound like hyperbole but consider this. In 2018, humans around the world produced more than 2.5 quintillion bytes of data—each day. According to some estimates , every minute people conduct almost 4.5 million Google searches, post 511,200 tweets, watch 4.5 million YouTube videos, swipe 1.4 million times on Tinder, and order 8,683 meals from GrubHub. These numbers—and the world’s total data—are expected to continue growing exponentially in the coming years.

For behavioral researchers and businesses, this data represents a valuable opportunity. However, using data to learn about human behavior or make decisions about consumer behavior often requires an understanding of statistics and statistical significance.

Statistical significance is a measurement of how likely it is that the difference between two groups, models, or statistics occurred by chance or occurred because two variables are actually related to each other. This means that a “statistically significant” finding is one in which it is likely the finding is real, reliable, and not due to chance.

To evaluate whether a finding is statistically significant, researchers engage in a process known as null hypothesis significance testing . Null hypothesis significance testing is less of a mathematical formula and more of a logical process for thinking about the strength and legitimacy of a finding.

Imagine a Vice President of Marketing asks her team to test a new layout for the company website. The new layout streamlines the user experience by making it easier for people to place orders and suggesting additional items to go along with each customer’s purchase. After testing the new website, the VP finds that visitors to the site spend an average of $12.63. Under the old layout, visitors spent an average of $12.32, meaning the new layout increases average spending by $0.31 per person. The question the VP must answer is whether the difference of $0.31 per person is significant or something that likely occurred by chance.

To answer this question with statistical analysis, the VP begins by adopting a skeptical stance toward her data known as the null hypothesis . The null hypothesis assumes that whatever researchers are studying does not actually exist in the population of interest. So, in this case the VP assumes that the change in website layout does not influence how much people spend on purchases.

With the null hypothesis in mind, the manager asks how likely it is that she would obtain the results observed in her study—the average difference of $0.31 per visitor—if the change in website layout actually causes no difference in people’s spending (i.e., if the null hypothesis is true). If the probability of obtaining the observed results is low, the manager will reject the null hypothesis and conclude that her finding is statistically significant.

Statistically significant findings indicate not only that the researchers’ results are unlikely the result of chance, but also that there is an effect or relationship between the variables being studied in the larger population. However, because researchers want to ensure they do not falsely conclude there is a meaningful difference between groups when in fact the difference is due to chance, they often set stringent criteria for their statistical tests. This criterion is known as the significance level .

Within the social sciences, researchers often adopt a significance level of 5%. This means researchers are only willing to conclude that the results of their study are statistically significant if the probability of obtaining those results if the null hypothesis were true—known as the p value —is less than 5%.

Five percent represents a stringent criterion, but there is nothing magical about it. In medical research, significance levels are often set at 1%. In cognitive neuroscience, researchers often adopt significance levels well below 1%. And, when astronomers seek to explain aspects of the universe or physicists study new particles like the Higgs Boson they set significance levels several orders of magnitude below .05.

In other research contexts like business or industry, researchers may set more lenient significance levels depending on the aim of their research. However, in all research, the more stringently a researcher sets their significance level, the more confident they can be that their results are not due to chance.

Determining whether a given set of results is statistically significant is only one half of the hypothesis testing equation. The other half is ensuring that the statistical tests a researcher conducts are powerful enough to detect an effect if one really exists. That is, when a researcher concludes their hypothesis was incorrect and there is no effect between the variables being studied, that conclusion is only meaningful if the study was powerful enough to detect an effect if one really existed.

The power of a hypothesis test is influenced by several factors.

Sample size—or, the number of participants the researcher collects data from—affects the power of a hypothesis test. Larger samples with more observations generally lead to higher-powered tests than smaller samples. In addition, large samples are more likely to produce replicable results because extreme scores that occur by chance are more likely to balance out in a large sample rather than in a small one.

Although setting a low significance level helps researchers ensure their results are not due to chance, it also lowers their power to detect an effect because it makes rejecting the null hypothesis harder. In this respect, the significance level a researcher selects is often in competition with power.

Standard deviations represent unexplained variability within data, also known as error. Generally speaking, the more unexplained variability within a dataset, the less power researchers have to detect an effect. Unexplained variability can be the result of measurement error, individual differences among participants, or situational noise.

A final factor that influences power is the size of the effect a researcher is studying. As you might expect, big changes in behavior are easier to detect than small ones.

Sometimes researchers do not know the strength of an effect before conducting a study. Even though this makes it harder to conduct a well powered study, it is important to keep in mind that phenomena that produce a large effect will lead to studies with more power than phenomena that produce only a small effect.

Statistical significance is important because it allows researchers to hold a degree of confidence that their findings are real, reliable, and not due to chance. But statistical significance is not equally important to all researchers in all situations. The importance of obtaining statistically significant results depends on what a researcher studies and within what context.

Within academic research, statistical significance is often critical because academic researchers study theoretical relationships between different variables and behavior. Furthermore, the goal of academic research is often to publish research reports in scientific journals. The threshold for publishing in academic journals is often a series of statistically significant results.

Outside of academia, statistical significance is often less important. Researchers, managers, and decision makers in business may use statistical significance to understand how strongly the results of a study should inform the decisions they make. But, because statistical significance is simply a way of quantifying how much confidence to hold in a research finding, people in industry are often more interested in a finding’s practical significance than statistical significance.

To demonstrate the difference between practical and statistical significance, imagine you’re a candidate for political office. Maybe you have decided to run for local or state-wide office, or, if you’re feeling bold, imagine you’re running for President.

During your campaign, your team comes to you with data on messages intended to mobilize voters. These messages have been market tested and now you and your team must decide which ones to adopt.

If you go with Message A, 41% of registered voters say they are likely to turn out at the polls and cast a ballot. If you go with Message B, this number drops to 37%. As a candidate, should you care whether this difference is statistically significant at a p value below .05?

The answer is of course not. What you likely care about more than statistical significance is practical significance —the likelihood that the difference between groups is large enough to be meaningful in real life.

You should ensure there is some rigor behind the difference in messages before you spend money on a marketing campaign, but when elections are sometimes decided by as little as one vote you should adopt the message that brings more people out to vote. Within business and industry, the practical significance of a research finding is often equally if not more important than the statistical significance. In addition, when findings have large practical significance, they are almost always statistically significant too.

Conducting statistically significant research is a challenge, but it’s a challenge worth tackling. Flawed data and faulty analyses only lead to poor decisions. Start taking steps to ensure your surveys and experiments produce valid results by using CloudResearch. If you have the team to conduct your own studies, CloudResearch can help you find large samples of online participants quickly and easily. Regardless of your demographic criteria or sample size, we can help you get the participants you need. If your team doesn’t have the resources to run a study, we can run it for you. Our team of expert social scientists, computer scientists, and software engineers can design any study, collect the data, and analyze the results for you. Let us show you how conducting statistically significant research can improve your decision-making today.

Continue Reading: A Researcher’s Guide to Statistical Significance and Sample Size Calculations

Part 2: How to Calculate Statistical Significance

Part 3: Determining Sample Size: How Many Survey Participants Do You Need?

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P-Value And Statistical Significance: What It Is & Why It Matters

Saul McLeod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul McLeod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

On This Page:

The p-value in statistics quantifies the evidence against a null hypothesis. A low p-value suggests data is inconsistent with the null, potentially favoring an alternative hypothesis. Common significance thresholds are 0.05 or 0.01.

P-Value Explained in Normal Distribution

Hypothesis testing

When you perform a statistical test, a p-value helps you determine the significance of your results in relation to the null hypothesis.

The null hypothesis (H0) states no relationship exists between the two variables being studied (one variable does not affect the other). It states the results are due to chance and are not significant in supporting the idea being investigated. Thus, the null hypothesis assumes that whatever you try to prove did not happen.

The alternative hypothesis (Ha or H1) is the one you would believe if the null hypothesis is concluded to be untrue.

The alternative hypothesis states that the independent variable affected the dependent variable, and the results are significant in supporting the theory being investigated (i.e., the results are not due to random chance).

What a p-value tells you

A p-value, or probability value, is a number describing how likely it is that your data would have occurred by random chance (i.e., that the null hypothesis is true).

The level of statistical significance is often expressed as a p-value between 0 and 1.

The smaller the p -value, the less likely the results occurred by random chance, and the stronger the evidence that you should reject the null hypothesis.

Remember, a p-value doesn’t tell you if the null hypothesis is true or false. It just tells you how likely you’d see the data you observed (or more extreme data) if the null hypothesis was true. It’s a piece of evidence, not a definitive proof.

Example: Test Statistic and p-Value

Suppose you’re conducting a study to determine whether a new drug has an effect on pain relief compared to a placebo. If the new drug has no impact, your test statistic will be close to the one predicted by the null hypothesis (no difference between the drug and placebo groups), and the resulting p-value will be close to 1. It may not be precisely 1 because real-world variations may exist. Conversely, if the new drug indeed reduces pain significantly, your test statistic will diverge further from what’s expected under the null hypothesis, and the p-value will decrease. The p-value will never reach zero because there’s always a slim possibility, though highly improbable, that the observed results occurred by random chance.

P-value interpretation

The significance level (alpha) is a set probability threshold (often 0.05), while the p-value is the probability you calculate based on your study or analysis.

A p-value less than or equal to your significance level (typically ≤ 0.05) is statistically significant.

A p-value less than or equal to a predetermined significance level (often 0.05 or 0.01) indicates a statistically significant result, meaning the observed data provide strong evidence against the null hypothesis.

This suggests the effect under study likely represents a real relationship rather than just random chance.

For instance, if you set α = 0.05, you would reject the null hypothesis if your p -value ≤ 0.05.

It indicates strong evidence against the null hypothesis, as there is less than a 5% probability the null is correct (and the results are random).

Therefore, we reject the null hypothesis and accept the alternative hypothesis.

Example: Statistical Significance

Upon analyzing the pain relief effects of the new drug compared to the placebo, the computed p-value is less than 0.01, which falls well below the predetermined alpha value of 0.05. Consequently, you conclude that there is a statistically significant difference in pain relief between the new drug and the placebo.

What does a p-value of 0.001 mean?

A p-value of 0.001 is highly statistically significant beyond the commonly used 0.05 threshold. It indicates strong evidence of a real effect or difference, rather than just random variation.

Specifically, a p-value of 0.001 means there is only a 0.1% chance of obtaining a result at least as extreme as the one observed, assuming the null hypothesis is correct.

Such a small p-value provides strong evidence against the null hypothesis, leading to rejecting the null in favor of the alternative hypothesis.

A p-value more than the significance level (typically p > 0.05) is not statistically significant and indicates strong evidence for the null hypothesis.

This means we retain the null hypothesis and reject the alternative hypothesis. You should note that you cannot accept the null hypothesis; we can only reject it or fail to reject it.

Note : when the p-value is above your threshold of significance, it does not mean that there is a 95% probability that the alternative hypothesis is true.

One-Tailed Test

Probability and statistical significance in ab testing. Statistical significance in a b experiments

Two-Tailed Test

How do you calculate the p-value ?

Most statistical software packages like R, SPSS, and others automatically calculate your p-value. This is the easiest and most common way.

Online resources and tables are available to estimate the p-value based on your test statistic and degrees of freedom.

These tables help you understand how often you would expect to see your test statistic under the null hypothesis.

Understanding the Statistical Test:

Different statistical tests are designed to answer specific research questions or hypotheses. Each test has its own underlying assumptions and characteristics.

For example, you might use a t-test to compare means, a chi-squared test for categorical data, or a correlation test to measure the strength of a relationship between variables.

Be aware that the number of independent variables you include in your analysis can influence the magnitude of the test statistic needed to produce the same p-value.

This factor is particularly important to consider when comparing results across different analyses.

Example: Choosing a Statistical Test

If you’re comparing the effectiveness of just two different drugs in pain relief, a two-sample t-test is a suitable choice for comparing these two groups. However, when you’re examining the impact of three or more drugs, it’s more appropriate to employ an Analysis of Variance ( ANOVA) . Utilizing multiple pairwise comparisons in such cases can lead to artificially low p-values and an overestimation of the significance of differences between the drug groups.

How to report

A statistically significant result cannot prove that a research hypothesis is correct (which implies 100% certainty).

Instead, we may state our results “provide support for” or “give evidence for” our research hypothesis (as there is still a slight probability that the results occurred by chance and the null hypothesis was correct – e.g., less than 5%).

Example: Reporting the results

In our comparison of the pain relief effects of the new drug and the placebo, we observed that participants in the drug group experienced a significant reduction in pain ( M = 3.5; SD = 0.8) compared to those in the placebo group ( M = 5.2; SD = 0.7), resulting in an average difference of 1.7 points on the pain scale (t(98) = -9.36; p < 0.001).

The 6th edition of the APA style manual (American Psychological Association, 2010) states the following on the topic of reporting p-values:

“When reporting p values, report exact p values (e.g., p = .031) to two or three decimal places. However, report p values less than .001 as p < .001.

The tradition of reporting p values in the form p < .10, p < .05, p < .01, and so forth, was appropriate in a time when only limited tables of critical values were available.” (p. 114)

Do not use 0 before the decimal point for the statistical value p as it cannot equal 1. In other words, write p = .001 instead of p = 0.001.
Please pay attention to issues of italics ( p is always italicized) and spacing (either side of the = sign).
p = .000 (as outputted by some statistical packages such as SPSS) is impossible and should be written as p < .001.
The opposite of significant is “nonsignificant,” not “insignificant.”

Why is the p -value not enough?

A lower p-value is sometimes interpreted as meaning there is a stronger relationship between two variables.

However, statistical significance means that it is unlikely that the null hypothesis is true (less than 5%).

To understand the strength of the difference between the two groups (control vs. experimental) a researcher needs to calculate the effect size .

When do you reject the null hypothesis?

In statistical hypothesis testing, you reject the null hypothesis when the p-value is less than or equal to the significance level (α) you set before conducting your test. The significance level is the probability of rejecting the null hypothesis when it is true. Commonly used significance levels are 0.01, 0.05, and 0.10.

Remember, rejecting the null hypothesis doesn’t prove the alternative hypothesis; it just suggests that the alternative hypothesis may be plausible given the observed data.

The p -value is conditional upon the null hypothesis being true but is unrelated to the truth or falsity of the alternative hypothesis.

What does p-value of 0.05 mean?

If your p-value is less than or equal to 0.05 (the significance level), you would conclude that your result is statistically significant. This means the evidence is strong enough to reject the null hypothesis in favor of the alternative hypothesis.

Are all p-values below 0.05 considered statistically significant?

No, not all p-values below 0.05 are considered statistically significant. The threshold of 0.05 is commonly used, but it’s just a convention. Statistical significance depends on factors like the study design, sample size, and the magnitude of the observed effect.

A p-value below 0.05 means there is evidence against the null hypothesis, suggesting a real effect. However, it’s essential to consider the context and other factors when interpreting results.

Researchers also look at effect size and confidence intervals to determine the practical significance and reliability of findings.

How does sample size affect the interpretation of p-values?

Sample size can impact the interpretation of p-values. A larger sample size provides more reliable and precise estimates of the population, leading to narrower confidence intervals.

With a larger sample, even small differences between groups or effects can become statistically significant, yielding lower p-values. In contrast, smaller sample sizes may not have enough statistical power to detect smaller effects, resulting in higher p-values.

Therefore, a larger sample size increases the chances of finding statistically significant results when there is a genuine effect, making the findings more trustworthy and robust.

Can a non-significant p-value indicate that there is no effect or difference in the data?

No, a non-significant p-value does not necessarily indicate that there is no effect or difference in the data. It means that the observed data do not provide strong enough evidence to reject the null hypothesis.

There could still be a real effect or difference, but it might be smaller or more variable than the study was able to detect.

Other factors like sample size, study design, and measurement precision can influence the p-value. It’s important to consider the entire body of evidence and not rely solely on p-values when interpreting research findings.

Can P values be exactly zero?

While a p-value can be extremely small, it cannot technically be absolute zero. When a p-value is reported as p = 0.000, the actual p-value is too small for the software to display. This is often interpreted as strong evidence against the null hypothesis. For p values less than 0.001, report as p < .001

Further Information

P Value Calculator From T Score
P-Value Calculator For Chi-Square
P-values and significance tests (Kahn Academy)
Hypothesis testing and p-values (Kahn Academy)
Wasserstein, R. L., Schirm, A. L., & Lazar, N. A. (2019). Moving to a world beyond “ p “< 0.05”.
Criticism of using the “ p “< 0.05”.
Publication manual of the American Psychological Association
Statistics for Psychology Book Download

Bland, J. M., & Altman, D. G. (1994). One and two sided tests of significance: Authors’ reply. BMJ: British Medical Journal , 309 (6958), 874.

Goodman, S. N., & Royall, R. (1988). Evidence and scientific research. American Journal of Public Health , 78 (12), 1568-1574.

Goodman, S. (2008, July). A dirty dozen: twelve p-value misconceptions . In Seminars in hematology (Vol. 45, No. 3, pp. 135-140). WB Saunders.

Lang, J. M., Rothman, K. J., & Cann, C. I. (1998). That confounded P-value. Epidemiology (Cambridge, Mass.) , 9 (1), 7-8.

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How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

An independent variable is something the researcher changes or controls.
A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. Ask a question

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

The relevant variables
The specific group being studied
The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

Research question	Hypothesis	Null hypothesis
What are the health benefits of eating an apple a day?	Increasing apple consumption in over-60s will result in decreasing frequency of doctor’s visits.	Increasing apple consumption in over-60s will have no effect on frequency of doctor’s visits.
Which airlines have the most delays?	Low-cost airlines are more likely to have delays than premium airlines.	Low-cost and premium airlines are equally likely to have delays.
Can flexible work arrangements improve job satisfaction?	Employees who have flexible working hours will report greater job satisfaction than employees who work fixed hours.	There is no relationship between working hour flexibility and job satisfaction.
How effective is high school sex education at reducing teen pregnancies?	Teenagers who received sex education lessons throughout high school will have lower rates of unplanned pregnancy teenagers who did not receive any sex education.	High school sex education has no effect on teen pregnancy rates.
What effect does daily use of social media have on the attention span of under-16s?	There is a negative between time spent on social media and attention span in under-16s.	There is no relationship between social media use and attention span in under-16s.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

Sampling methods
Simple random sampling
Stratified sampling
Cluster sampling
Likert scales
Reproducibility

Statistics

Null hypothesis
Statistical power
Probability distribution
Effect size
Poisson distribution

Research bias

Optimism bias
Cognitive bias
Implicit bias
Hawthorne effect
Anchoring bias
Explicit bias

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A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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Statistics By Jim

Making statistics intuitive

Hypothesis Testing and Confidence Intervals

By Jim Frost 20 Comments

Confidence intervals and hypothesis testing are closely related because both methods use the same underlying methodology. Additionally, there is a close connection between significance levels and confidence levels. Indeed, there is such a strong link between them that hypothesis tests and the corresponding confidence intervals always agree about statistical significance.

A confidence interval is calculated from a sample and provides a range of values that likely contains the unknown value of a population parameter . To learn more about confidence intervals in general, how to interpret them, and how to calculate them, read my post about Understanding Confidence Intervals .

In this post, I demonstrate how confidence intervals work using graphs and concepts instead of formulas. In the process, I compare and contrast significance and confidence levels. You’ll learn how confidence intervals are similar to significance levels in hypothesis testing. You can even use confidence intervals to determine statistical significance.

Read the companion post for this one: How Hypothesis Tests Work: Significance Levels (Alpha) and P-values . In that post, I use the same graphical approach to illustrate why we need hypothesis tests, how significance levels and P-values can determine whether a result is statistically significant, and what that actually means.

Significance Level vs. Confidence Level

Let’s delve into how confidence intervals incorporate the margin of error. Like the previous post, I’ll use the same type of sampling distribution that showed us how hypothesis tests work. This sampling distribution is based on the t-distribution , our sample size , and the variability in our sample. Download the CSV data file: FuelsCosts .

There are two critical differences between the sampling distribution graphs for significance levels and confidence intervals–the value that the distribution centers on and the portion we shade.

The significance level chart centers on the null value, and we shade the outside 5% of the distribution.

Conversely, the confidence interval graph centers on the sample mean, and we shade the center 95% of the distribution.

Probability distribution plot that displays 95% confidence interval for our fuel cost dataset.

The shaded range of sample means [267 394] covers 95% of this sampling distribution. This range is the 95% confidence interval for our sample data. We can be 95% confident that the population mean for fuel costs fall between 267 and 394.

Confidence Intervals and the Inherent Uncertainty of Using Sample Data

The graph emphasizes the role of uncertainty around the point estimate . This graph centers on our sample mean. If the population mean equals our sample mean, random samples from this population (N=25) will fall within this range 95% of the time.

We don’t know whether our sample mean is near the population mean. However, we know that the sample mean is an unbiased estimate of the population mean. An unbiased estimate does not tend to be too high or too low. It’s correct on average. Confidence intervals are correct on average because they use sample estimates that are correct on average. Given what we know, the sample mean is the most likely value for the population mean.

Given the sampling distribution, it would not be unusual for other random samples drawn from the same population to have means that fall within the shaded area. In other words, given that we did, in fact, obtain the sample mean of 330.6, it would not be surprising to get other sample means within the shaded range.

If these other sample means would not be unusual, we must conclude that these other values are also plausible candidates for the population mean. There is inherent uncertainty when using sample data to make inferences about the entire population. Confidence intervals help gauge the degree of uncertainty, also known as the margin of error.

Confidence Intervals and Statistical Significance

If you want to determine whether your hypothesis test results are statistically significant, you can use either P-values with significance levels or confidence intervals. These two approaches always agree.

The relationship between the confidence level and the significance level for a hypothesis test is as follows:

Confidence level = 1 – Significance level (alpha)

For example, if your significance level is 0.05, the equivalent confidence level is 95%.

Both of the following conditions represent statistically significant results:

The P-value in a hypothesis test is smaller than the significance level.
The confidence interval excludes the null hypothesis value.

Further, it is always true that when the P-value is less than your significance level, the interval excludes the value of the null hypothesis.

In the fuel cost example, our hypothesis test results are statistically significant because the P-value (0.03112) is less than the significance level (0.05). Likewise, the 95% confidence interval [267 394] excludes the null hypotheses value (260). Using either method, we draw the same conclusion.

Hypothesis Testing and Confidence Intervals Always Agree

The hypothesis testing and confidence interval results always agree. To understand the basis of this agreement, remember how confidence levels and significance levels function:

A confidence level determines the distance between the sample mean and the confidence limits.
A significance level determines the distance between the null hypothesis value and the critical regions.

Both of these concepts specify a distance from the mean to a limit. Surprise! These distances are precisely the same length.

A 1-sample t-test calculates this distance as follows:

The critical t-value * standard error of the mean

Interpreting these statistics goes beyond the scope of this article. But, using this equation, the distance for our fuel cost example is $63.57.

P-value and significance level approach : If the sample mean is more than $63.57 from the null hypothesis mean, the sample mean falls within the critical region, and the difference is statistically significant.

Confidence interval approach : If the null hypothesis mean is more than $63.57 from the sample mean, the interval does not contain this value, and the difference is statistically significant.

Of course, they always agree!

The two approaches always agree as long as the same hypothesis test generates the P-values and confidence intervals and uses equivalent confidence levels and significance levels.

Related posts : Standard Error of the Mean and Critical Values

I Really Like Confidence Intervals!

In statistics, analysts often emphasize using hypothesis tests to determine statistical significance. Unfortunately, a statistically significant effect might not always be practically meaningful. For example, a significant effect can be too small to be important in the real world. Confidence intervals help you navigate this issue!

Similarly, the margin of error in a survey tells you how near you can expect the survey results to be to the correct population value.

Learn more about this distinction in my post about Practical vs. Statistical Significance .

Learn how to use confidence intervals to compare group means !

Finally, learn about bootstrapping in statistics to see an alternative to traditional confidence intervals that do not use probability distributions and test statistics. In that post, I create bootstrapped confidence intervals.

Neyman, J. (1937). Outline of a Theory of Statistical Estimation Based on the Classical Theory of Probability . Philosophical Transactions of the Royal Society A . 236 (767): 333–380.

Reader Interactions

December 7, 2021 at 3:14 pm

I am helping my Physics students use their data to determine whether they can say momentum is conserved. One of the columns in their data chart was change in momentum and ultimately we want this to be 0. They are obviously not getting zero from their data because of outside factors. How can I explain to them that their data supports or does not support conservation of momentum using statistics? They are using a 95% confidence level. Again, we want the change in momentum to be 0. Thank you.

December 9, 2021 at 6:54 pm

I can see several complications with that approach and also my lack of familiarity with the subject area limits what I can say. But here are some considerations.

For starters, I’m unsure whether the outside factors you mention bias the results systematically from zero or just add noise (variability) to the data (but not systematically bias).

If the outside factors bias the results to a non-zero value, then you’d expect the case where larger samples will be more likely to produce confidence intervals that exclude zero. Indeed, only smaller samples sizes might produce CIs that include zero, but that would only be due to the relative lack of precision associated with small sample sizes. In other words, limited data won’t be able to distinguish the sample value from zero even though, given the bias of the outside factors, you’d expect a non-zero value. In other words, if the bias exists, the larger samples will detect the non-zero values correctly while smaller samples might miss it.

If the outside factors don’t bias the results but just add noise, then you’d expect that both small and larger samples will include zero. However, you still have the issue of precision. Smaller samples will include zero because they’re relatively wider intervals. Larger samples should include zero but have narrower intervals. Obviously, you can trust the larger samples more.

In hypothesis testing, when you fail to reject the null, as occurs in the unbiased discussion above, you’re not accepting the null . Click the link to read about that. Failing to reject the null does not mean that the population value equals the hypothesized value (zero in your case). That’s because you can fail to reject the null due to poor quality data (high noise and/or small sample sizes). And you don’t want to draw conclusions based on poor data.

There’s a class of hypothesis testing called equivalence testing that you should use in this case. It flips the null and alternative hypotheses so that the test requires you to collect strong evidence to show that the sample value equals the null value (again, zero in your case). I don’t have a post on that topic (yet), but you can read the Wikipedia article about Equivalence Testing .

I hope that helps!

September 19, 2021 at 5:16 am

Thank you very much. When training a machine learning model using bootstrap, in the end we will have the confidence interval of accuracy. How can I say that this result is statistically significant? Do I have to convert the confidence interval to p-values first and if p-value is less than 0.05, then it is statistically significant?

September 19, 2021 at 3:16 pm

As I mention in this article, you determine significance using a confidence interval by assessing whether it excludes the null hypothesis value. When it excludes the null value, your results are statistically significant.

September 18, 2021 at 12:47 pm

Dear Jim, Thanks for this post. I am new to hypothesis testing and would like to ask you how we know that the null hypotheses value is equal to 260.

Thank you. Kind regards, Loukas

September 19, 2021 at 12:35 am

For this example, the null hypothesis is 260 because that is the value from the previous year and they wanted to compare the current year to the previous year. It’s defined as the previous year value because the goal of the study was to determine whether it has changed since last year.

In general, the null hypothesis will often be a meaningful target value for the study based on their knowledge, such as this case. In other cases, they’ll use a value that represents no effect, such as zero.

I hope that helps clarify it!

February 22, 2021 at 3:49 pm

Hello, Mr. Jim Frost.

Thank you for publishing precise information about statistics, I always read your posts and bought your excellent e-book about regression! I really learn from you.

I got a couple of questions about the confidence level of the confidence intervals. Jacob Cohen, in his article “things I’ve learned (so far)” said that, in his experience, the most useful and informative confidence level is 80%; other authors state that if that level is below 90% it would be very hard to compare across results, as it is uncommon.

My first question is: in exploratory studies, with small samples (for example, N=85), if one wishes to generate correlational hypothesis for future research, would it be better to use a lower confidence level? What is the lowest level you would consider to be acceptable? I ask that because of my own research now, and with a sample size 85 (non-probabilistic sampling) I know all I can do is generate some hypothesis to be explored in the future, so I would like my confidence intervals to be more informative, because I am not looking forward to generalize to the population.

My second question is: could you please provide an example of an appropriate way to describe the information about the confidence interval values/limits, beyond the classic “it contains a difference of 0; it contains a ratio of 1”.

I would really appreciate your answers.

Greetings from Peru!

February 23, 2021 at 4:51 pm

Thanks so much for your kind words and for supporting my regression ebook! I’m glad it’s been helpful! 🙂

On to your questions!

I haven’t read Cohen’s article, so I don’t understand his rationale. However, I’m extremely dubious of using a confidence level as low as 80%. Lowering the confidence level will create a narrower CI, which looks good. However, it comes at the expense of dramatically increasing the likelihood that the CI won’t contain the correct population value! My position is to leave the confidence level at 95%. Or, possibly lower it to 90%. But, I wouldn’t go further. Your CI will be wider, but that’s OK. It’s reflecting the uncertainty that truly exists in your data. That’s important. The problem with lowering the CIs is that it makes your results appear more precise than they actually are.

When I think of exploratory research, I think of studies that are looking at tendencies or trends. Is the overall pattern of results consistent with theoretical expectations and justify further research? At that stage, it shouldn’t be about obtaining statistically significant results–at least not as the primary objective. Additionally, exploratory research can help you derive estimated effect sizes, variability, etc. that you can use for power calculations . A smaller, exploratory study can also help you refine your methodology and not waste your resources by going straight to a larger study that, as a result, might not be as refined as it would without a test run in the smaller study. Consequently, obtaining significant results, or results that look precise when they aren’t, aren’t the top priorities.

I know that lowering the confidence level makes your CI look more information but that is deceptive! I’d resist that temptation. Maybe go down to 90%. Personally, I would not go lower.

As for the interpretation, CIs indicate the likely range that a population parameter is likely to fall within. The parameter can be a mean, effect size, ratio, etc. Often times, you as the researcher are hoping the CI excludes an important value. For example, if the CI is of the effect size, you want the CI to exclude zero (no effect). In that case, you can say that there is unlikely to be no effect in the population (i.e., there probably is a non-zero effect in the population). Additionally, the effect size is likely to be within this range. Other times, you might just want to know the range of values itself. For example, if you have a CI for the mean height of a population, it might be valuable on its own knowing that the population mean height is likely to fall between X and Y. If you have specific example of what the CI assesses, I can give you a more specific interpretation.

Additionally, I cover confidence intervals associated with many different types of hypothesis tests in my Hypothesis Testing ebook . You might consider looking in to that!

July 26, 2020 at 5:45 am

I got a very wide 95% CI of the HR of height in the cox PH model from a very large sample. I already deleted the outliers defined as 1.5 IQR, but it doesn’t work. Do you know how to resolve it?

July 5, 2020 at 6:13 pm

Hello, Jim!

I appreciate the thoughtful and thorough answer you provided. It really helped in crystallizing the topic for me.

If I may ask for a bit more of your time, as long as we are talking about CIs I have another question:

How would you go about constructing a CI for the difference of variances?

I am asking because while creating CIs for the difference of means or proportions is relatively straightforward, I couldn’t find any references for the difference of variances in any of my textbooks (or on the Web for that matter); I did find information regarding CIs for the ratio of variances, but it’s not the same thing.

Could you help me with that?

Thanks a lot!

July 2, 2020 at 6:01 pm

I want to start by thanking you for a great post and an overall great blog! Top notch material.

I have a doubt regarding the difference between confidence intervals for a point estimate and confidence intervals for a hypothesis test.

As I understand, if we are using CIs to test a hypothesis, then our point estimate would be whatever the null hypothesis is; conversely, if we are simply constructing a CI to go along with our point estimate, we’d use the point estimate derived from our sample. Am I correct so far?

The reason I am asking is that because while reading from various sources, I’ve never found a distinction between the two cases, and they seem very different to me.

Bottom line, what I am trying to ask is: assuming the null hypothesis is true, shouldn’t the CI be changed?

Thank you very much for your attention!

July 3, 2020 at 4:02 pm

There’s no difference in the math behind the scenes. The real difference is that when you create a confidence interval in conjunction with a hypothesis test, the software ensures that they’re using consistent methodology. For example, the significance level and confidence level will correspond correctly (i.e., alpha = 0.05 and confidence level = 0.95). Additionally, if you perform a two-tailed test, you will obtain a two-sided CI. On the other hand, if you perform a one-tailed test, you will obtain the appropriate upper or lower bound (i.e., one-sided CIs). The software also ensures any other methodological choices you make will match between the hypothesis test and CI, which ensures the results always agree.

You can perform them separately. However, if you don’t match all the methodology options, the results can differ.

As for your question about assuming the null is true. Keep in mind that hypothesis tests create sampling distributions that center on the null hypothesis value. That’s the assumption that the null is true. However, the sampling distributions for CIs center on the sample estimate. So, yes, CIs change that detail because they don’t assume the null is correct. But that’s always true whether you perform the hypothesis test or not.

Thanks for the great questions!

December 21, 2019 at 6:31 am

Confidence interval has sample static as the most likely value ( value in the center) – and sample distribution assumes the null value to be the most likely value( value in the center). I am a little confused about this. Would be really kind of you if you could show both in the same graph and explain how both are related. How the the distance from the mean to a limit in case of Significance level and CI same?

December 23, 2019 at 3:46 am

That’s a great question. I think part of your confusion is due to terminology.

The sampling distribution of the means centers on the sample mean. This sampling distribution uses your sample mean as its mean and the standard error of the mean as its standard deviation.

The sampling distribution of the test statistic (t) centers on the null hypothesis value (0). This distribution uses zero as its mean and also uses the SEM for its standard deviation.

They’re two different things and center on different points. But, they both incorporate the SEM, which is why they always agree! I do have section in this post about why that distance is always the same. Look for the section titled, “Why They Always Agree.”

November 23, 2019 at 11:31 pm

Hi Jim, I’m the proud owner of 2 of your ebooks. There’s one topic though that keeps puzzling me: If I would take 9 samples of size 15 in order to estimate the population mean, the se of the mean would be substantial larger than if I would take 1 sample of size 135 (divide pop sd by sqrt(15) or sqrt(135) ) whereas the E(x) (or mean of means) would be the same.

Can you please shine a little light on that.

Tx in advance

November 24, 2019 at 3:17 am

Thanks so much for supporting my ebooks. I really appreciate that!! 🙂

So, let’s flip that scenario around. If you know that a single large sample of 135 will produce more precise estimates of the population, why would you collect nine smaller samples? Knowing how statistics works, that’s not a good decision. If you did that in the real world, it would be because there was some practical reason that you could not collect one big example. Further, it would suggest that you had some reason for not being able to combine them later. For example, if you follow the same random sampling procedure on the same population and used all the same methodology and at the same general time, you might feel comfortable combining them together into one larger sample. So, if you couldn’t collect one larger example and you didn’t feel comfortable combining them together, it suggests that you have some reason for doubting that they all measure the same thing for the same population. Maybe you had differences in methodology? Or subjective measurements across different personnel? Or, maybe you collected the samples at different times and you’re worried that the population changed over time?

So, that’s the real world reason for why a researcher would not combine smaller samples into a larger one.

As you can see, the expected value for the population standard deviation is in the numerator (sigma). As the sample size increases, the numerator remains constant (plus or minus random error) because the expected value for the population parameter does not change. Conversely, the square root of the sample size is in the denominator. As the sample size increases, it produces a larger values in the denominator. So, if the expected value of the numerator is constant but the value of the denominator increases with a larger sample size, you expect the SEM to decrease. Smaller SEM’s indicate more precise estimates of the population parameter. For instance, the equations for confidence intervals use the SEM. Hence, for the same population, larger samples tend to produce smaller SEMS, and more precise estimates of the population parameter.

I hope that answers your question!

November 6, 2018 at 10:26 am

first of all: Thanks for your effort and your effective way of explaining!

You say that p-values and C.I.s always agree. I agree.

Why does Tim van der Zee claim the opposite? I’m not enough into statistcs to figure this out.

http://www.timvanderzee.com/not-interpret-confidence-intervals/

Best regards Georg

November 7, 2018 at 9:31 am

I think he is saying that they do agree–just that people often compare the wrong pair of CIs and p-values. I assume you’re referring to the section “What do overlapping intervals (not) mean?” And, he’s correct in what he says. In a 2-sample t-test, it’s not valid to compare the CI for each of the two group means to the test’s p-values because they have different purposes. Consequently, they won’t necessarily agree. However, that’s because you’re comparing results from two different tests/intervals.

On the one hand, you have the CIs for each group. On the other hand, you have the p-value for the difference between the two groups. Those are not the same thing and so it’s not surprising that they won’t agree necessarily.

However, if you compare the p-value of the difference between means to a CI of the difference between means, they will always agree. You have to compare apples to apples!

April 14, 2018 at 8:54 pm

First of all, I love all your posts and you really do make people appreciate statistics by explaining it intuitively compared to theoretical approaches I’ve come across in university courses and other online resources. Please continue the fantastic work!!!

At the end, you mentioned how you prefer confidence intervals as they consider both “size and precision of the estimated effect”. I’m confused as to what exactly size and precision mean in this context. I’d appreciate an explanation with reference to specific numbers from the example above.

Second, do p-values lack both size and precision in determination of statistical significance?

Thanks, Devansh

April 17, 2018 at 11:41 am

Hi Devansh,

Thanks for the nice comments. I really appreciate them!

I really need to write a post specifically about this issue.

Let’s first assume that we conduct our study and find that the mean cost is 330.6 and that we are testing whether that is different than 260. Further suppose that we perform the the hypothesis test and obtain a p-value that is statistically significant. We can reject the null and conclude that population mean does not equal 260. And we can see our sample estimate is 330.6. So, that’s what we learn using p-values and the sample estimate.

Confidence intervals add to that information. We know that if we were to perform the experiment again, we’d get different results. How different? Is the true population mean likely to be close to 330.6 or further away? CIs help us answer these questions. The 95% CI is [267 394]. The true population value is likely to be within this range. That range spans 127 dollars.

However, let’s suppose we perform the experiment again but this time use a much larger sample size and obtain a mean of 351 and again a significant p-value. However, thanks to the large sample size, we obtain a 95 CI of [340 362]. Now we know that the population value is likely to fall within this much tighter interval of only 22 dollars. This estimate is much more precise.

Sometimes you can obtain a significant p-value for a result that is too imprecise to be useful. For example, with first CI, it might be too wide to be useful for what we need to do with our results. Maybe we’re helping people make budgets and that is too wide to allow for practical planning. However, the more precise estimate of the second study allows for better budgetary planning! That determination how much precision is required must be made using subject-area knowledge and focusing on the practical usage of the results. P-values don’t indicate the precision of the estimates in this manner!

I hope this helps clarify this precision issue!

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Teach yourself statistics

Hypothesis Test: Difference Between Means

This lesson explains how to conduct a hypothesis test for the difference between two means. The test procedure, called the two-sample t-test , is appropriate when the following conditions are met:

The sampling method for each sample is simple random sampling .
The samples are independent .
Each population is at least 20 times larger than its respective sample .
The population distribution is normal.
The population data are symmetric , unimodal , without outliers , and the sample size is 15 or less.
The population data are slightly skewed , unimodal, without outliers, and the sample size is 16 to 40.
The sample size is greater than 40, without outliers.

This approach consists of four steps: (1) state the hypotheses, (2) formulate an analysis plan, (3) analyze sample data, and (4) interpret results.

State the Hypotheses

Every hypothesis test requires the analyst to state a null hypothesis and an alternative hypothesis . The hypotheses are stated in such a way that they are mutually exclusive. That is, if one is true, the other must be false; and vice versa.

The table below shows three sets of null and alternative hypotheses. Each makes a statement about the difference d between the mean of one population μ 1 and the mean of another population μ 2 . (In the table, the symbol ≠ means " not equal to ".)

Set	Null hypothesis	Alternative hypothesis	Number of tails
1	μ - μ = d	μ - μ ≠ d	2
2	μ - μ d	μ - μ < d	1
3	μ - μ d	μ - μ > d	1

The first set of hypotheses (Set 1) is an example of a two-tailed test , since an extreme value on either side of the sampling distribution would cause a researcher to reject the null hypothesis. The other two sets of hypotheses (Sets 2 and 3) are one-tailed tests , since an extreme value on only one side of the sampling distribution would cause a researcher to reject the null hypothesis.

When the null hypothesis states that there is no difference between the two population means (i.e., d = 0), the null and alternative hypothesis are often stated in the following form.

H o : μ 1 = μ 2

H a : μ 1 ≠ μ 2

Formulate an Analysis Plan

The analysis plan describes how to use sample data to accept or reject the null hypothesis. It should specify the following elements.

Significance level. Often, researchers choose significance levels equal to 0.01, 0.05, or 0.10; but any value between 0 and 1 can be used.
Test method. Use the two-sample t-test to determine whether the difference between means found in the sample is significantly different from the hypothesized difference between means.

Analyze Sample Data

Using sample data, find the standard error, degrees of freedom, test statistic, and the P-value associated with the test statistic.

SE = sqrt[ (s 1 2 /n 1 ) + (s 2 2 /n 2 ) ]

DF = (s 1 2 /n 1 + s 2 2 /n 2 ) 2 / { [ (s 1 2 / n 1 ) 2 / (n 1 - 1) ] + [ (s 2 2 / n 2 ) 2 / (n 2 - 1) ] }

t = [ ( x 1 - x 2 ) - d ] / SE

P-value. The P-value is the probability of observing a sample statistic as extreme as the test statistic. Since the test statistic is a t statistic, use the t Distribution Calculator to assess the probability associated with the t statistic, having the degrees of freedom computed above. (See sample problems at the end of this lesson for examples of how this is done.)

Interpret Results

If the sample findings are unlikely, given the null hypothesis, the researcher rejects the null hypothesis. Typically, this involves comparing the P-value to the significance level , and rejecting the null hypothesis when the P-value is less than the significance level.

Test Your Understanding

In this section, two sample problems illustrate how to conduct a hypothesis test of a difference between mean scores. The first problem involves a two-tailed test; the second problem, a one-tailed test.

Problem 1: Two-Tailed Test

Within a school district, students were randomly assigned to one of two Math teachers - Mrs. Smith and Mrs. Jones. After the assignment, Mrs. Smith had 30 students, and Mrs. Jones had 25 students.

At the end of the year, each class took the same standardized test. Mrs. Smith's students had an average test score of 78, with a standard deviation of 10; and Mrs. Jones' students had an average test score of 85, with a standard deviation of 15.

Test the hypothesis that Mrs. Smith and Mrs. Jones are equally effective teachers. Use a 0.10 level of significance. (Assume that student performance is approximately normal.)

Solution: The solution to this problem takes four steps: (1) state the hypotheses, (2) formulate an analysis plan, (3) analyze sample data, and (4) interpret results. We work through those steps below:

State the hypotheses. The first step is to state the null hypothesis and an alternative hypothesis.

Null hypothesis: μ 1 - μ 2 = 0

Alternative hypothesis: μ 1 - μ 2 ≠ 0

Formulate an analysis plan . For this analysis, the significance level is 0.10. Using sample data, we will conduct a two-sample t-test of the null hypothesis.

SE = sqrt[(s 1 2 /n 1 ) + (s 2 2 /n 2 )]

SE = sqrt[(10 2 /30) + (15 2 /25] = sqrt(3.33 + 9)

SE = sqrt(12.33) = 3.51

DF = (10 2 /30 + 15 2 /25) 2 / { [ (10 2 / 30) 2 / (29) ] + [ (15 2 / 25) 2 / (24) ] }

DF = (3.33 + 9) 2 / { [ (3.33) 2 / (29) ] + [ (9) 2 / (24) ] } = 152.03 / (0.382 + 3.375) = 152.03/3.757 = 40.47

t = [ ( x 1 - x 2 ) - d ] / SE = [ (78 - 85) - 0 ] / 3.51 = -7/3.51 = -1.99

where s 1 is the standard deviation of sample 1, s 2 is the standard deviation of sample 2, n 1 is the size of sample 1, n 2 is the size of sample 2, x 1 is the mean of sample 1, x 2 is the mean of sample 2, d is the hypothesized difference between the population means, and SE is the standard error.

Since we have a two-tailed test , the P-value is the probability that a t statistic having 40 degrees of freedom is more extreme than -1.99; that is, less than -1.99 or greater than 1.99.

We use the t Distribution Calculator to find P(t < -1.99) is about 0.027.

If you enter 1.99 as the sample mean in the t Distribution Calculator, you will find the that the P(t ≤ 1.99) is about 0.973. Therefore, P(t > 1.99) is 1 minus 0.973 or 0.027. Thus, the P-value = 0.027 + 0.027 = 0.054.
Interpret results . Since the P-value (0.054) is less than the significance level (0.10), we cannot accept the null hypothesis.

Note: If you use this approach on an exam, you may also want to mention why this approach is appropriate. Specifically, the approach is appropriate because the sampling method was simple random sampling, the samples were independent, the sample size was much smaller than the population size, and the samples were drawn from a normal population.

Problem 2: One-Tailed Test

The Acme Company has developed a new battery. The engineer in charge claims that the new battery will operate continuously for at least 7 minutes longer than the old battery.

To test the claim, the company selects a simple random sample of 100 new batteries and 100 old batteries. The old batteries run continuously for 190 minutes with a standard deviation of 20 minutes; the new batteries, 200 minutes with a standard deviation of 40 minutes.

Test the engineer's claim that the new batteries run at least 7 minutes longer than the old. Use a 0.05 level of significance. (Assume that there are no outliers in either sample.)

Null hypothesis: μ 1 - μ 2 <= 7

Alternative hypothesis: μ 1 - μ 2 > 7

where μ 1 is battery life for the new battery, and μ 2 is battery life for the old battery.

Formulate an analysis plan . For this analysis, the significance level is 0.05. Using sample data, we will conduct a two-sample t-test of the null hypothesis.

SE = sqrt[(40 2 /100) + (20 2 /100]

SE = sqrt(16 + 4) = 4.472

DF = (40 2 /100 + 20 2 /100) 2 / { [ (40 2 / 100) 2 / (99) ] + [ (20 2 / 100) 2 / (99) ] }

DF = (20) 2 / { [ (16) 2 / (99) ] + [ (2) 2 / (99) ] } = 400 / (2.586 + 0.162) = 145.56

t = [ ( x 1 - x 2 ) - d ] / SE = [(200 - 190) - 7] / 4.472 = 3/4.472 = 0.67

Here is the logic of the analysis: Given the alternative hypothesis (μ 1 - μ 2 > 7), we want to know whether the observed difference in sample means is big enough (i.e., sufficiently greater than 7) to cause us to reject the null hypothesis.

Interpret results . Suppose we replicated this study many times with different samples. If the true difference in population means were actually 7, we would expect the observed difference in sample means to be 10 or less in 75% of our samples. And we would expect to find an observed difference to be more than 10 in 25% of our samples Therefore, the P-value in this analysis is 0.25.

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7.3 Inference for Two-Sample Proportions

$\hat{p}_{1}$

When conducting inference on two independent population proportions, the following characteristics should be present:

The two independent samples are simple random samples that are independent.
For each of the samples, the number of successes is at least five, and the number of failures is at least five.
Growing literature states that the population must be at least ten or 20 times the size of the sample. This keeps each population from being over-sampled and causing incorrect results.

Sampling Distribution of the Difference in Two Proportions

Hypothesis test for the difference in two proportions.

If two estimated proportions are different, it may be due to a difference in the populations, or it may be due to chance. A hypothesis test can help determine if a difference in the estimated proportions reflects a difference in the population proportions.

Generally, the null hypothesis states that the two proportions are the same (i.e., H 0 : p 1 = p 2 ). Since we are assuming there is no difference in the null, we can use both samples to estimate the pooled proportion, p p , calculated as follows:

$\frac{{x}_{1}+{x}_{2}}{{n}_{1}+{n}_{2}}$

We can use this pooled proportion in the calculation of our z -test statistic:

$\frac{\left({\hat{p}}_{1}-{\hat{p}}_{2}\right)-\left({p}_{1}-{p}_{2}\right)}{\sqrt{{p}_{p}\left(1-{p}_{p}\right)\left(\frac{1}{{n}_{1}}+\frac{1}{{n}_{2}}\right)}}$

Two types of medication for hives are being tested to determine if there is a difference in the proportions of adult patient reactions. Twenty out of a random sample of 200 adults given Medication A still had hives 30 minutes after taking the medication. Twelve out of another random sample of 200 adults given Medication B still had hives 30 minutes after taking the medication. Test at a 1% level of significance.

Normal distribution curve of the difference in the percentages of adult patients who don't react to medication A and B after 30 minutes. The mean is equal to zero, and the values -0.04, 0, and 0.04 are labeled on the horizontal axis. Two vertical lines extend from -0.04 and 0.04 to the curve. The region to the left of -0.04 and the region to the right of 0.04 are each shaded to represent 1/2(p-value) = 0.0702.

Two types of valves are being tested to determine if there is a difference in pressure tolerances. For Valve A, 15 out of a random sample of 100 cracked under 4,500 psi. For Valve B, six out of a random sample of 100 cracked under 4,500 psi. Test at a 5% level of significance.

Confidence Intervals for the Difference in Two Proportions

$\left({z}_{\frac{\alpha }{2}}\right)\left(SE)$

Putting that all together, our formula for a CI to estimate the difference in two proportions will be:

$\hat{p}_{2}\pm\left({z}_{\frac{\alpha }{2}}\right)\sqrt{\frac{\hat{p}_{1}\text{(1-}\hat{p}_{1})}{n_1}+\frac{\hat{p}_{2}\text{(1-}\hat{p}_{2})}{n_2}}$

Click here for more multimedia resources, including podcasts, videos, lecture notes, and worked examples.

Figure References

Figure 7.8: Kindred Grey (2020). Medications A and B. CC BY-SA 4.0.

The probability distribution of a statistic at a given sample size

Estimate of the common value of p1 and p2

An interval built around a point estimate for an unknown population parameter

Significant Statistics Copyright © 2024 by John Morgan Russell, OpenStaxCollege, OpenIntro is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License , except where otherwise noted.

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COMMENTS

An Easy Introduction to Statistical Significance (With Examples)
Significance is usually denoted by a p-value, or probability value. Statistical significance is arbitrary - it depends on the threshold, or alpha value, chosen by the researcher. The most common threshold is p < 0.05, which means that the data is likely to occur less than 5% of the time under the null hypothesis.
Understanding Hypothesis Tests: Significance Levels (Alpha) and P
Alternative hypothesis: The population mean differs from the hypothesized mean (260). What Is the Significance Level (Alpha)? The significance level, also denoted as alpha or α, is the probability of rejecting the null hypothesis when it is true. For example, a significance level of 0.05 indicates a 5% risk of concluding that a difference ...
Understanding Significance Levels in Statistics
Most scholars define that evidentiary standard as being 90%, 95%, or even 99% sure that the defendant is guilty. In statistics, the significance level is the evidentiary standard. For researchers to successfully make the case that the effect exists in the population, the sample must contain a sufficient amount of evidence.
How Hypothesis Tests Work: Significance Levels (Alpha) and P values
However, it doesn't really explain what statistical significance means. I find that comparing the p-value to the significance level is the easy part. Knowing what it means and how to choose your significance level is the harder part! ... Null hypothesis: The population mean mu=260 equals the null hypothesis mean x-bar (330.6).
5.6 Hypothesis Tests in Depth
Statistical Significance vs. Practical Significance. When the sample size becomes larger, point estimates become more precise and any real differences in the mean and null value become easier to detect and recognize. Even a very small difference would likely be detected if we took a large enough sample.
5.5 Introduction to Hypothesis Tests
In this section, you will conduct hypothesis tests on single means when the population standard deviation is known. Hypothesis testing consists of two contradictory hypotheses or statements, a decision based on the data, and a conclusion. To perform a hypothesis test, a statistician will perform some variation of these steps: Define hypotheses.
Statistical Significance: Definition & Meaning
The definition of statistically significant is that the sample effect is unlikely to be caused by chance (i.e., sampling error). In other words, what we see in the sample likely reflects an effect or relationship that exists in the population. Using a more statistically correct technical definition, statistical significance relates to the ...
What Is Statistical Significance & Why Learn It
The significance level of a hypothesis test is the threshold you use to determine whether or not your results are statistically significant. The significance level is the probability of falsely rejecting the null hypothesis when it is actually true. The most commonly used significance levels are: 0.10 (or 10%)
Hypothesis Testing, P Values, Confidence Intervals, and Significance
Medical providers often rely on evidence-based medicine to guide decision-making in practice. Often a research hypothesis is tested with results provided, typically with p values, confidence intervals, or both. Additionally, statistical or research significance is estimated or determined by the investigators. Unfortunately, healthcare providers may have different comfort levels in interpreting ...
Hypothesis Testing
Step 5: Present your findings. The results of hypothesis testing will be presented in the results and discussion sections of your research paper, dissertation or thesis.. In the results section you should give a brief summary of the data and a summary of the results of your statistical test (for example, the estimated difference between group means and associated p-value).
Hypothesis Testing
Let's return finally to the question of whether we reject or fail to reject the null hypothesis. If our statistical analysis shows that the significance level is below the cut-off value we have set (e.g., either 0.05 or 0.01), we reject the null hypothesis and accept the alternative hypothesis. Alternatively, if the significance level is above ...
Tests of Significance
Significance Levels The significance level for a given hypothesis test is a value for which a P-value less than or equal to is considered statistically significant. Typical values for are 0.1, 0.05, and 0.01. These values correspond to the probability of observing such an extreme value by chance. In the test score example above, the P-value is 0.0082, so the probability of observing such a ...
An Explanation of P-Values and Statistical Significance
The textbook definition of a p-value is: A p-value is the probability of observing a sample statistic that is at least as extreme as your sample statistic, given that the null hypothesis is true. For example, suppose a factory claims that they produce tires that have a mean weight of 200 pounds. An auditor hypothesizes that the true mean weight ...
Level of Significance & Hypothesis Testing
The level of significance is defined as the criteria or threshold value based on which one can reject the null hypothesis or fail to reject the null hypothesis. The level of significance determines whether the outcome of hypothesis testing is statistically significant or otherwise. The significance level is also called as alpha level.
What Does It Mean for Research to Be Statistically Significant?
This means that a "statistically significant" finding is one in which it is likely the finding is real, reliable, and not due to chance. To evaluate whether a finding is statistically significant, researchers engage in a process known as null hypothesis significance testing. Null hypothesis significance testing is less of a mathematical ...
Understanding P-Values and Statistical Significance
This means we retain the null hypothesis and reject the alternative hypothesis. You should note that you cannot accept the null hypothesis; we can only reject it or fail to reject it. Note : when the p-value is above your threshold of significance, it does not mean that there is a 95% probability that the alternative hypothesis is true.
PDF Tests of Significance
A test of significance is a formal procedure for comparing observed data with a claim (also called a hypothesis), the truth of which is being assessed. • The claim is a statement about a parameter, like the population proportion p or the population mean µ. • The results of a significance test are expressed in terms of a probability that
10.26: Hypothesis Test for a Population Mean (5 of 5)
The hypotheses are claims about the population mean, µ. The null hypothesis is a hypothesis that the mean equals a specific value, µ 0. The alternative hypothesis is the competing claim that µ is less than, greater than, or not equal to the . When is < or > , the test is a one-tailed test. When is ≠ , the test is a two-tailed test.
Introduction to Hypothesis Testing
Hypothesis Tests. A hypothesis test consists of five steps: 1. State the hypotheses. State the null and alternative hypotheses. These two hypotheses need to be mutually exclusive, so if one is true then the other must be false. 2. Determine a significance level to use for the hypothesis. Decide on a significance level.
How to Write a Strong Hypothesis
The specific group being studied. The predicted outcome of the experiment or analysis. 5. Phrase your hypothesis in three ways. To identify the variables, you can write a simple prediction in if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.
10.29: Hypothesis Test for a Difference in Two Population Means (1 of 2)
Step 1: Determine the hypotheses. The hypotheses for a difference in two population means are similar to those for a difference in two population proportions. The null hypothesis, H 0, is again a statement of "no effect" or "no difference.". H 0: μ 1 - μ 2 = 0, which is the same as H 0: μ 1 = μ 2. The alternative hypothesis, H a ...
Hypothesis Testing and Confidence Intervals
The relationship between the confidence level and the significance level for a hypothesis test is as follows: Confidence level = 1 - Significance level (alpha) For example, if your significance level is 0.05, the equivalent confidence level is 95%. Both of the following conditions represent statistically significant results: The P-value in a ...
Hypothesis Test: Difference in Means
The first step is to state the null hypothesis and an alternative hypothesis. Null hypothesis: μ 1 - μ 2 = 0. Alternative hypothesis: μ 1 - μ 2 ≠ 0. Note that these hypotheses constitute a two-tailed test. The null hypothesis will be rejected if the difference between sample means is too big or if it is too small.
6.2 Inference for the Mean in Practice
Statistics students believe that the mean score on the first statistics test is 65. A statistics instructor thinks the mean score is higher than 65. He samples ten statistics students and obtains the scores below: 65, 65, 70, 67, 66, 63, 63, 68, 72, 71. Perform the hypothesis test using a 5% level of significance to test the instructor's claim.
Significant Differences in Sepal Length Across Iris Species
Statistics document from Purdue University, 10 pages, HW-7 Ishita Shukla 2023-11-03 Q1> Null Hypothesis: There is no significant difference in means of Sepal.Length across different species of iris. Alternative Hypothesis: There is a significant difference in means of Sepal.Length across different species of
7.3 Inference for Two-Sample Proportions
If two estimated proportions are different, it may be due to a difference in the populations, or it may be due to chance. A hypothesis test can help determine if a difference in the estimated proportions reflects a difference in the population proportions. Generally, the null hypothesis states that the two proportions are the same (i.e., H 0: p ...