phd in tissue engineering

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Tissue Engineering

Tissue Engineering is the field of research using cells and other materials to either enhance or replace biological tissues. To that end, many faculty in BE are studying in this field including one who is using stem cell-seeded scaffolds to repair degraded cartilage and another who has engineered mice to fluorescently display genetic changes.

Laurie A. Boyer, PhD

Ron weiss, phd, harvey f. lodish, phd, c. forbes dewey, jr., phd, ed boyden, phd, robert langer, scd, darrell j. irvine, phd, douglas a. lauffenburger, phd, alan j. grodzinsky, scd, roger d. kamm, phd.

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Tissue Engineering and Regenerative Medicine

Research in tissue engineering and regenerative medicine seeks to replace or regenerate diseased or damaged tissues, organs, and cells – a challenging endeavor, but one that has tremendous potential for the practice of medicine.

Technologies under investigation range from biomaterial/cell constructs for repairing various tissues and organs, to stem cell therapies, to immune therapies. Our work in this area is highly multidisciplinary, combining materials science, cell biology, clinical science, immunology, stem cell biology, genome science, and others.

Accordingly, researchers in this area within Duke BME are broadly interactive with departments throughout the university including Duke University Medical Center clinical departments, the Duke University School of Medicine departments of Cell Biology and Immunology, the Duke Department of Chemistry, and others. This community is also supported by centers and programs such as Regeneration Next and the Center for Biomolecular and Tissue Engineering (CBTE) .

Primary Faculty

Nenad Bursac

Professor of Biomedical Engineering

Research Interests: Embryonic and adult stem cell therapies for heart and muscle disease; cardiac and skeletal muscle tissue engineering; cardiac electrophysiology and arrhythmias; genetic modifications of stem and somatic cells; micropatterning of proteins and hydrogels.

Pranam D. Chatterjee

Assistant Professor of Biomedical Engineering

Research Interests: Integration of computational and experimental methodologies to design novel proteins for applications in genome editing, targeted protein modulation, and reproductive bioengineering

Joel Collier

Theodore Kennedy Professor of Biomedical Engineering

Research Interests: The design of biomaterials for a range of biomedical applications, with a focus on understanding and controlling adaptive immune responses. Most materials investigated are created from molecular assemblies- proteins, peptides or bioconjugates that self-organize into useful…

Sharon Gerecht

Paul M. Gross Distinguished Professor of Biomedical Engineering

Research Interests: stem cells, biomaterials, hypoxia, blood vessels, physics of cancer, regenerative medicine

Charles Gersbach

John W. Strohbehn Distinguished Professor of Biomedical Engineering

Research Interests: Gene therapy, genomics and epigenomics, biomolecular and cellular engineering, regenerative medicine, and synthetic biology.

John Wirthlin Hickey

Samira Musah

Assistant Professor in the Department of Biomedical Engineering

Research Interests: Induced pluripotent stem cells (iPS cells), disease mechanisms, regenerative medicine, molecular and cellular basis of human kidney development and disease, organ engineering, patient-specific disease models, biomarkers, therapeutic discovery, tissue and organ transplantation,…

Tatiana Segura

Research Interests: The design of biomaterials to promote endogenous repair and reducing inflammation through the design of the geometry of the material, and delivering genes, proteins and drugs.

George A. Truskey

R. Eugene and Susie E. Goodson Distinguished Professor of Biomedical Engineering

Research Interests: Cardiovascular tissue engineering, mechanisms of atherogenesis, cell adhesion, and cell biomechanics.

Shyni Varghese

Professor of Biomedical Engineering, Mechanical Engineering & Materials Science and Orthopaedics

Research Interests: Musculoskeletal tissue repair, disease biophysics and organ-on-a-chip technology

Secondary Faculty

Geoffrey Steven Ginsburg

Adjunct Professor in the Department of Medicine

Cynthia Ann Toth

Joseph A.C. Wadsworth Distinguished Professor of Ophthalmology

Stefan Zauscher

Professor in the Thomas Lord Department of Mechanical Engineering and Materials Science

Research Interests: Nano-mechanical and nano-tribological characterization (elasticity, friction, adhesion) of materials including organic thin films; self-assembled monolayers, polymeric gels, and cellulosics; Fabrication of polymeric nanostructures by scanning probe lithography; Colloidal probe…

Adjunct Faculty

Jennifer L West

Adjunct Professor of Biomedical Engineering

Research Interests: Biomaterials, nanotechnology and tissue engineering that involves the synthesis, development, and application of novel biofunctional materials, and the use of biomaterials and engineering approaches to study biological problems.

Faculty Emeritus

William M. Reichert

Professor Emeritus of Biomedical Engineering

Research Interests: Biosensors, protein mediated cell adhesion, and wound healing.

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BME Tissue Engineering & Biomaterials

Cornell biomedical engineers design biomaterial platforms to recreate tissues for functional replacement therapies, as models of normal and diseased states for basic research, and for use in drug testing.

Well-controlled and biocompatible biomaterials are also needed for selective delivery of therapeutic and imaging contrast agents as well as for gene therapy approaches. Critical to the success of Cornell’s tissue engineering and biomaterials efforts is the integration of multidisciplinary expertise in materials science, cell biology, biochemistry, and biomechanics. Center facilities supporting this research include: the Cornell Center for Materials Research, Cornell NanoScale Science and Technology Facility, the Nanobiotechnology Center, and the NIH-funded Physical Sciences Oncology Center (Center on the Physics of Cancer Metabolism). Many projects are joint with faculty at Weill Cornell Medicine. 

Faculty research interests

Lawrence Bonassar

[email protected]

Lawrence Bonassar ’s lab focuses on the development of anatomically shaped cartilage for applications in musculoskeletal repair. Researchers in the lab use medical imaging data combined with 3D tissue printing and molding technology to generate engineered tissues for replacement of articular and auricular cartilage, meniscus, and intervertebral disc, as well as use mechanical conditioning in bioreactors to guide the development of desired microstructure in engineered tissues.

Jonathan Butcher

[email protected]

Jonathan Butcher ’s lab creates and uses living 3D culture models of heart valve physiology and disease. He employs engineering principles from developmental biology to drive the differentiation of stem/progenitor cells towards mature cardiac and valvular phenotypes. Dr. Butcher has also pioneered the use of 3D tissue printing to fabricate living heterogeneous soft tissues. He combines tunable, 3D printable hydrogel inks and novel deposition algorithms for precision design and fabrication of patient-specific heterogeneous clinically sized grafts.

Ben Cosgrove

[email protected]

Ben Cosgrove ’s lab studies how muscle stem cells interpret and process microenvironmental information through signaling networks that govern their cell fate during tissue maintenance and repair. Inspired by these regulatory insights, his lab develops self-assembled and microfabricated biomaterials to engineered muscle-mimetic tissues and enhance muscle stem cell transplantation therapies.

Claudia Fischbach

[email protected]

Claudia Fischbach ’s lab studies the effect of microenvironmental conditions on the prognosis and treatment of cancer patients. Her lab combines biomaterials, tissue engineering, and microfabrication strategies to develop pathologically relevant culture models for analysis of tumor-mediated angiogenesis, stroma remodeling, and bone metastasis.

Jan Lammerding

[email protected]

Jan Lammerding ’s lab is using a combination of microfabrication, tissue-engineering, decellularization, and stem cell biology to create in vitro models of skeletal and cardiac muscle, with the goal to elucidate how mutations in nuclear envelope proteins such as lamin A/C and emerin cause muscular dystrophies and heart disease.

Esak (Isaac) Lee

[email protected]

Esak (Isaac) Lee ’s lab creates tissue engineered vessels-on-chip platforms that recapitulate lymphatic and blood vascular structure and function to ultimately use the platforms for tissue regeneration. Blood perfusion is important for implanting large-scale tissue constructs and lymphatic drainage is another key factor in tissue fluid homeostasis. Dr. Lee hypothesizes that adding lymphatics to the pre-vascularized in vivo patch may improve tissue function.

Marjolein van der Meulen

[email protected]

Marjolein van der Meule n’s research focuses on musculoskeletal mechanobiology and tissue mechanics. Her laboratory studies adaptation to mechanical loading during development, maintenance, disease and repair of bone and other musculoskeletal tissues. Her lab is also interested in the determinants of whole bone strength and skeletal load-bearing function based on microscale tissue properties. Experiments in her laboratory combine in vivo models with in vitro testing, imaging and computational simulations.

Yadong Wang

[email protected]

Yadong Wang ’s group focuses on creating biomaterials that will solve key challenges in the cardiovascular, nervous and musculoskeletal systems. His team enjoys collaboration with others who share the same passion for translational research.

BME Graduate Field Faculty in Tissue Engineering & Biomaterials

Susan Daniel , [email protected]  Lara Estroff , [email protected] Brian Kirby , [email protected] Dan Luo , [email protected] Minglin Ma , [email protected] Suzanne Maher , [email protected] Alexander Nikitin , [email protected] Chris Ober , [email protected] Matthew Paszek , [email protected] Robert E. Schwartz, [email protected] Ulrich Wiesner , [email protected] Timothy Wright , [email protected] Rong Yang , [email protected]

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UC Berkeley Department of Bioengineering

The future of biology. The future of engineering.

research_head

Cell & Tissue Engineering

Cell and tissue engineering centers on the application of physical and engineering principles to understand and control cell and tissue behavior. Cellular engineering focuses on cell-level phenomena, while tissue engineering and regenerative medicine seek to generate or stimulate new tissue for disease treatment. 

Two areas in which the department has established special leadership are cellular mechanobiology, which focuses on understanding the interaction and conversion between force-based and biochemical information in living systems, and stem cell engineering, which includes platforms to expand, implant, and mobilize stem cells for tissue repair and replacement.

Faculty working in cell & tissue engineering: 

Dean A. Richard Newton Memorial Professor, Bioengineering; Senior Faculty Scientist, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory; Director, Center for the Utilization of Bioengineering in Space; CEO/CSO, DOE Systems Biology Knowledgebase PI and Co-Director, ENIGMA SFA

​The Arkin Lab focus is how microbes transform, clean, and improve soils, soils that are currently degrading due to climate change, pollution, and poor water use. Near close-loops, low-energy, low-input biomanufacturing programs for food, pharmaceuticals, and building materials at “small village” scale, which are initially designed for a deep-space crewed Mars mission but have applications here on Earth for supporting sustainable agriculture. Another interest is to develop engineering approaches for microbiomes so we can control communities of microbes that drive the earth’s mineral cycles, support our plants and efficiency and stress responses, and impact the health and food-efficiency of a good many living creatures including ourselves.

Professor, Bioengineering Professor, Mechanical Engineering

Theory and applications of solid mechanics to traditional materials and biomaterials.

Professor, Bioengineering

Our work has been focused on establishing new paradigms in multi-tissue stem cell aging, rejuvenation and regulation by conserved morphogenic signaling pathways. One of our goals is to define pharmacology for enhancing maintenance and repair of adult tissues in vivo. The spearheaded by us heterochronic parabiosis and blood apheresis studies have established that the process of aging is reversible through modulation of circulatory milieu. Our synthetic biology method of choice focuses on bio-orthogonal non-canonical amino acid tagging (BONCAT) and subsequent identification of age-imposed and disease-causal changes in mammalian proteomes in vivo. Our drug delivery reg medicine projects focus on CRISPR/Cas9 based therapeutics for more effective and safer gene editing.

Assistant Professor, Bioengineering

​The development of immunoengineering technologies to direct immune cell function. We build artificial lymph nodes, mRNA vaccines and 3D printed interfaces to study and control immune cell behaviour. These technologies have applications in cancer therapy, inducing transplant tolerance, spaceflight and auto-immune diseases.

Purnendu Chatterjee Chair in Engineering Biological Systems, Bioengineering Faculty Scientist, Lawrence Berkeley National Laboratory

The Fletcher Lab develops diagnostic technologies and studies mechanical regulation of membrane and cytoskeleton organization in the context of cell motility, signaling, and host-pathogen interactions. We specialize in development of optical microscopy, force microscopy, and microfluidic technologies to understand fundamental organizational principles through both in vitro reconstitution and live cell experiments. Recent work includes investigating the mechano-biochemistry of branched actin network assembly with force microscopy, studying membrane deformation by protein crowding and oligomerization with model membranes, and reconstituting spindle scaling in encapsulated cytoplasmic extracts. The long-term goal of our work is to understand and harness spatial organization for therapeutic applications in cancer and infectious diseases.

Jan Fandrianto Professor, Bioengineering Professor, Materials Science & Engineering

Research in the Healy Lab emphasizes the relationship between materials and the cells or tissues they contact. The research program focuses on the design and synthesis of bioinspired materials that actively direct the fate of mammalian cells, and facilitate regeneration of damaged tissues and organs. Major discoveries from his laboratory have centered on the control of cell fate and tissue formation in contract with materials that are tunable in both their biological content and mechanical properties. Professor Healy also has extensive experience with human stem cell technologies, microphysiological systems, drug delivery systems, and novel bioconjugate therapeutics.

Professor in Residence, Professor of Orthopaedic Surgery and Bioengineering & Therapeutic Sciences, UCSF Director, Health Innovations Via Engineering (HIVE), UCSF

Dr. Hernandez’s research in biomechanics examines the musculoskeletal system, microscopic organisms and interactions between microbes and materials. Current projects include understanding how the microbiome influences bone and infection of total joint replacements, how bacteria are influenced by mechanical stress and strain, and engineered living materials.

Adjunct Professor, Bioengineering

Chancellors Professor, Bioengineering Chancellors Professor, Mechanical Engineering

Biomechanics of cortical and trabecular bone; design of spine prostheses; bone fracture and osteoporosis; tissue engineering of bone.

Chancellor’s Professor, Bioengineering & Chemical and Biomolecular Engineering Director, California Institute for Quantitative Biosciences (QB3) at UC Berkeley Professor in Residence, Bioengineering and Therapeutic Sciences, UCSF Faculty Scientist, Biological Systems and Engineering, LBNL

Our lab seeks to understand and engineer mechanical and other biophysical communication between cells and materials. In addition to investigating fundamental aspects of this problem with a variety of micro/nanoscale technologies, we are especially interested in discovering how this signaling regulates tumor and stem cell biology in the central nervous system. Recent directions have included: (1) Engineering new tissue-mimetic culture platforms for biophysical studies, molecular analysis, and screening; (2) Exploring mechanobiological signaling systems as targets for limiting the invasion of brain tumors and enhancing stem cell neurogenesis; and (3) Creating new biomaterials inspired by cellular structural networks.

Chair of Bioengineering, Class of 1941 WWII Memorial Chair in Bioengineering and Materials Science and Engineering

My laboratory is interested in understanding structure-property relationships in biological materials and in using this information to design biologically inspired materials for use in healthcare. Fundamental studies include single molecule and bulk biophysical studies of biointerfacial and bulk mechanochemical phenomena in biological materials, whereas our applied studies the design and synthesis of novel biomaterials for tissue repair and regeneration.

Professor, Bioengineering Professor, Mechanical Engineering Faculty Scientist, Lawrence Berkeley National Lab

​Molecular and Multiscale Biomechanics; Bioinformatics and Computational Biology; Statistical Machine Learning; Computational Precision Health; Microbiome; Personalized Medicine

Our laboratory is focused on developing new materials for drug delivery and molecular imaging.

Professor, Mechanical Engineering Lawrence Talbot Professor, Mechanical Engineering

Characterization of structural evolution in medical grade ultra high molecular weight poliethylene due to sterilization: the implications for total joint replacements.

Professor Emeritus, Bioengineering Professor Emeritus, Medicine, UCSF

The research focus is on hand and arm biomechanics and the design of workplace tools and tasks in order to improve productivity and the quality of work while preventing upper extremity fatigue and injury. The lab has studied designs of tablets, gesture interfaces, keyboards, mice, pipettors, touch screens, dental tools, construction drills, chairs, and agricultural tools. Funding is primarily from NIH and CDC but also from Hewlett-Packard, Microsoft, BART, Logitech, and Herman-Miller.

Professor Emeritus, Bioengineering Professor of the Graduate School, Mechanical Engineering

Bioelectronic devices, biotransport, medical imaging, electrical impedance tomography.

Professor, Chemical & Biomolecular Engineering, Bioengineering, and Molecular & Cell Biology Executive Director, QB3 Director, Bakar Labs and the Bakar BioEnginuity Hub Director, Berkeley Stem Cell Center

Our research program melds basic biology and applied engineering principles to investigate preclinical and clinical gene and stem cell therapies, i.e. gene replacement and cell replacement approaches to treat human disease.

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Bioengineering

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Bioengineering is a field within the engineering sciences area of study at the Harvard John A. Paulson School of Engineering and Applied Sciences. Prospective students apply through the Harvard Kenneth C. Griffin Graduate of School of Arts and Sciences (Harvard Griffin GSAS). In the online application, select “Engineering and Applied Sciences” as your program choice and select “PhD Engineering Sciences: Bioengineering” in the area of study menu.

The bioengineering program is an interdisciplinary program that provides you an opportunity to interact with many areas of the University and Harvard-affiliated teaching hospitals. You will learn how bioengineering integrates fundamental engineering disciplines such as thermodynamics and fluid mechanics with the physical and life sciences while drawing on mathematics and computational sciences. This convergence will enable you to understand the operation of living systems that leads to the design of novel solutions to address critical problems in medicine and biology.

Bioengineers at Harvard are making advances in bio-inspired robotics and computing, biometrics and motor control, cell and tissue engineering, biomaterials, and therapeutics. Examples of projects current and past students have worked on include embedding stretchable nanoelectronics into brain organoids to study brain development and developing injectable clotting agents to reduce blood loss.

Graduates of the program have gone on to a range of careers in industry in companies like McKinsey & Company and Medtronic. Others have positions in academia at MIT, Vanderbilt, and Stanford.

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Tissue Engineering

The field of tissue engineering developed to address the shortage of tissues available for repair and transplantation strategies. At Yale, we are working to develop functional engineered tissues for vascular grafts and arteries, spinal cord repair, liver transplantation, and immune engineering.

The team of researchers from the School of Engineering & Applied Science and the Medical School meet regularly as part of the Tissue Engineering Group to discuss their work. The collaborations between the clinicians and basic scientists are making the translation of tissue engineering technologies possible.

Dr. Breuer is working to engineer vascular grafts for pediatric patients. Dr. Niklason is developing engineered arteries for coronary bypass procedures. Dr. Kyriakides is looking at how the materials used in these structures affect the body and the healing process. Dr. Madri is using novel tissue engineered scaffolds for nerve regeneration. Drs. Saltzman, Pober, and Bothwell are working toward the creation of engineered pancreatic islets. We have experts in polymers, scaffold fabrication, drug delivery systems, nanotechnology, cellular and molecular biology working together to engineer new three-dimensional tissues.

Tissue engineering requires great collaborations and expertise in surgery, biomaterials, cell biology, and drug delivery. The collaborations in the Tissue Engineering Group and Vascular Biology and Transplantation Program bring together the scientists, engineers, surgeons, and physicians to develop new approaches to tissue replacement and repair.

Faculty involved with research:

W. Mark Saltzman – BME – ChE & EnvE

Jay Humphrey – BME Laura Niklason – BME – Anesthesiology

Themis R. Kyriakides – BME – Pathology Tarek Fahmy – BME – ChE & EnvE

Anjelica Gonzalez – BME Jordan Pober – Immunobiology

Paul Van Tassel – ChE & EnvE

Alfred Bothwell – Immunobiology

Institute for Stem Cell & Regenerative Medicine

Tissue engineering.

These are the faculty members that are specialized in tissue engineering.

Nancy Allbritton, MD, PhD (Bioengineering) Research in my laboratory focuses on the development of novel methods and technologies to answer fundamental questions in biology & medicine.  Much of biology & medicine is technology limited in that leaps in knowledge follow closely on the heels of new discoveries and inventions in the physical and engineering sciences; consequently, interdisciplinary groups which bridge these different disciplines are playing increasingly important roles in biomedical research.  Our lab has developed partnerships with other investigators in the areas of biology, medicine, chemistry, physics, and engineering to design, fabricate, test, and utilize new tools for biomedical and clinical research.  Collaborative projects include novel strategies to measure enzyme activity in single cells using microelectrophoresis innovations, to build organ-on-a-chips particularly intestine-on-chip, array-based methods for cell screening and sorting.  An additional focus area is the development of software and instrumentation to support these applications areas. The ultimate goal is to design and build novel technologies and then translate these technologies into the marketplace to insure their availability to the biomedical research and clinical communities to enable humans to lead healthier and more productive lives.

Cole A. DeForest, PhD  (Chemical Engineering) While the potential for biomaterial-based strategies to improve and extend the quality of human health through tissue regeneration and the treatment of disease continues to grow, the majority of current strategies rely on outdated technology initially developed and optimized for starkly different applications. Therefore, the DeForest Group seeks to integrate the governing principles of rational design with fundamental concepts from material science, synthetic chemistry, and stem cell biology to conceptualize, create, and exploit next-generation materials to address a variety of health-related problems. We are currently interested in the development of new classes of user-programmable hydrogels whose biochemical and biophysical properties can be tuned in time and space over a variety of scales. Our work relies heavily on the utilization of cytocompatible bioorthogonal chemistries, several of which can be initiated with light and thereby confined to specific sub-volumes of a sample. By recapitulating the dynamic nature of the native tissue through 4D control of the material properties, these synthetic environments are utilized to probe and better understand basic cell function as well as to engineer complex heterogeneous tissue.

David A. Dichek, MD (Medicine/Cardiology) Our work focuses on defining the molecular mechanisms that drive aortic aneurysm formation and that precipitate atherosclerotic plaque rupture (the proximal cause of most heart attacks). We are also developing a gene therapy—delivered to the blood vessel wall—that prevents and reverses atherosclerosis. Experiments are performed in a mouse model of heritable thoracic aortic aneurysms, a mouse model of atherosclerotic plaque rupture, and with advanced human plaque tissue. Our gene therapy research uses helper-dependent adenoviral vectors to test therapies in rabbit models of carotid artery and vein graft atherosclerosis.  We anticipate that insights from our work will lead to therapies that prevent or stabilize aortic aneurysms and that prevent and reverse atherosclerosis.

Benjamin Freedman, PhD  (Medicine/Nephrology) Our laboratory has developed techniques to efficiently differentiate hPSCs into kidney organoids in a reproducible, multi-well format – a prototype ‘kidney-in-a-dish’. In addition, we have generated hPSC lines carrying naturally occurring or engineered mutations relevant to human kidney diseases, such as polycystic kidney disease and nephrotic syndrome. The goal of our research is to use these new tools to model human kidney disease and identify therapeutic approaches, including kidney regeneration.

Cecilia Giachelli, PhD  (Bioengineering) My lab is interested in applying stem cell and regenerative medicine strategies to the areas of ectopic calcification, tissue engineering, biomaterials development and biocompatibility.

Ray Monnat, PhD  (Pathology and Genome Sciences) Our research focuses on human RecQ helicase deficiency syndromes such as Werner syndrome; high resolution analyses of DNA replication dynamics; and the engineering of homing endonucleases for targeted gene modification or repair in human and other animal cells.

Tracy E. Popowics, PhD (Oral Health Sciences) Our team focusses on regeneration of the periodontal ligament (PDL) that maintains tooth position and provides support during chewing. Our approach is to engineer three-dimensional (3D) periodontal constructs that mimic the native tissue structure and function. Our 3D PDL constructs include cells that are suspended in collagen matrix and recreate the living PDL tissue. Periodontal tissue loss not only includes loss of the ligament, but also the alveolar bone and cementum that anchor the periodontal ligament and hold the tooth in place. This tissue loss may occur to different degrees during an individual’s lifespan due to changes in oral care, periodontal disease, systemic disease or other health problems. This is particularly true for the aged population in which diminished oral care can contribute to persistent and recurring periodontal inflammation and tissue breakdown. Regenerating these three layers is essential to restore the structural and functional integrity of PDL and to prevent tooth loss.

Feini (Sylvia) Qu, VMD, PhD (Orthopaedics & Sports Medicine, Mechanical Engineering) The long-term goal of our research is to understand the cellular and molecular mechanisms of musculoskeletal tissue regeneration, especially with respect to the bones and connective tissues of limbs and joints, and then leverage this knowledge to regenerate lost or diseased structures using stem cells, gene editing, and biomaterials. Our lab uses the mouse digit tip, one of the few mammalian systems that exhibits true regeneration, to identify pathways that regulate tissue patterning and outgrowth after amputation. Armed with a better understanding of the cues that direct complex tissue formation in adulthood, we will develop therapeutic strategies that enhance the regeneration of limbs and joints after injury and degenerative disease in patients.

Buddy Ratner, PhD  (Bioengineering) Stem cells proliferate and differentiate in response to micromechanical cues, surface biological signals, orientational directives and chemical gradients. To control stem cell proliferation and differentiation, the Ratner lab brings 30 years experience in surface control of biology, polymer scaffold fabrication and controlled release of bioactive agents to address the challenges of directing stem cell differentiation and subsequent tissue formation.

Michael Regnier, PhD  (Bioengineering) The Regnier lab works in a highly collaborative environment to develop both cell replacement and gene therapies approaches to treat diseased and failing hearts and skeletal muscle. Cell replacement strategies include development and testing of tissue engineered constructs. Gene therapies are target and improve myofilament contractile protein function.

Jenny Robinson, PhD (Orthopaedics & Sports Medicine and Mechanical Engineering) Our primary goal is to understand what cues are needed to promote connective tissue (ligament, cartilage, fibrocartilage) regeneration after knee injuries and reduce the onset of osteoarthritis. We have a particular interest on how these cues may differ in male and female athletes. We engineer biomaterial-based environments that mimic native tissue biochemical and mechanical properties to pinpoint specific cues that are required for regeneration of the connective tissues in the knee. We aim to use this knowledge to inform the treatment options for patients with knee injuries to ensure they can get back to performance with reduced or minimal chance for the development of osteoarthritis.

Shelly Sakiyama-Elbert, PhD (Bioengineering) Our lab works on developing novel approaches to treat peripheral nerve and spinal cord injury.  We use stem cell derived neurons and glia for transplantation following injury to replace cells that are lost as well as model systems to test potential drugs to promote regeneration.  Our ultimate goal is to provide patients with new therapies that will improve functional outcomes after injury.

Mehmet Sarikaya, PhD  (Materials Science and Engineering) Our research focuses on Molecular Biomimetics in which we use combinatorial mutagenesis to select peptides with specific affinity to desired materials, use bioinformatics-based pathways to in-silico design peptides, tailor their structure and function using genetic engineering protocols, couple them with synthetic self-assembled molecular hybrids, and use them as molecular tools in practical medicine and materials technologies. Our focus at the biology/materials interface incorporates molecular biology and nanotechnology, computational biology and bioinformatics, molecular assemblers, bio-enabled nanophotonics (quantum-dot and surface-enhanced probes), and peptide-based matrices for neural, dental and soft tissue regeneration.

Drew L. Sellers, PhD  (Bioengineering) Despite possessing a resident pool of neural stem cells, the mammalian brain and spinal cord shows a limited ability to regenerate damaged tissue after traumatic injury.  Instead, injury initiates a cascade of events that direct reactive gliosis to wall off an injury with a glial scar to mitigate damage and preserve function. My current research interests explore approaches to re-engineer the stem cell niche, to utilize gene-therapy and genome editing approaches to reprogram and engineer stem cells directly, and to enhance drug delivery into the central nervous system (CNS) to drive regenerative strategies that augment functional recovery in the diseased or traumatically injured CNS.

Alec Smith, PhD (Physiology & Biophysics) My lab’s research is focused on understanding the mechanistic pathways that underpin muscle and nervous tissue development in health and disease. To achieve this, we are developing human stem cell-derived models of neuromuscular diseases, such as amyotrophic lateral sclerosis (ALS). By analyzing the behavior of these cells, we aim to better define how the causal mutation leads to the development and progression of neurodegenerative disease. Ultimately, identification of pathways critical to disease progression will provide new targets for therapeutic intervention, leading to the development of new treatments for patients suffering from these debilitating and life-threatening conditions.

Nathan Sniadecki, PhD   (Mechanical Engineering) Our mission is to understand how mechanics affects human biology and disease at the cellular level. If we can formulate how cells are guided by mechanics, then we can direct cellular response in order to engineer cells and tissue for medical applications. We specialize in the design and development of micro- and nano-tools, which allows us to probe the role of cell mechanics at a length scale appropriate to the size of cells and their proteins.

Kelly R. Stevens, PhD  (Bioengineering and Pathology) Our research is focused on developing new technologies to assemble synthetic human tissues from stem cells, and to remotely control these tissues after implantation in a patient. To do this, we use diverse tools from stem cell biology, tissue engineering, synthetic biology, microfabrication, and bioprinting. We seek to translate our work into new regenerative therapies for patients with heart and liver disease.

Thomas N. Wight, PhD  (Benaroya Research Institute) This investigator leads a research program focused on the role that the extracellular matrix molecules, proteoglycans and hyaluronan, play in regulating vascular cell type and the regulation of extracellular matrix assembly. These pathways are fundamental to understanding the growth of new blood vessels in different tissues of the body, and have potential for direct tissue regeneration applications through the use of proteoglycan genes to bioengineer vascular tissue.

Ying Zheng, PhD  (Bioengineering) Dr. Zheng’s research focuses on understanding and engineering the fundamental structure and functions in living tissue and organ systems from nanometer, micrometer to centimeter scale.

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Tissue engineering articles from across Nature Portfolio

Tissue engineering is a set of methods that can replace or repair damaged or diseased tissues with natural, synthetic, or semisynthetic tissue mimics. These mimics can either be fully functional or will grow into the required functionality.

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Modulation of Adhesion and Migration of NIH/3T3 Cells in Collagen Materials by Taxifolin Derivatives

• Published: 17 January 2024
• Volume 17 , pages S85–S93, ( 2023 )

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• Yu. V. Shatalin 1 ,
• M. I. Kobyakova 1 , 2 &
• V. S. Shubina 1  

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One of the urgent tasks of tissue engineering is the development of stable non-toxic materials that support cell migration during tissue regeneration. This study was aimed at obtaining new gel materials based on collagen and derivatives of taxifolin, taxifolin pentaglutarate and a conjugate of taxifolin with glyoxylic acid and investigating their properties. It was shown that an increase in the proportion of polyphenols in the gel led to a decrease in the rate of degradation of the material. The obtained materials did not negatively affect the viability of NIH/3T3 mouse fibroblasts. The cells were attached to the surface of the materials and spread out on the surface of the material containing taxifolin pentaglutarate. It was also found that fibroblasts migrated through the obtained materials. An increase in the proportion of the conjugate of taxifolin with glyoxylic acid in the material led to inhibition of migration through the material, whereas an increase in the proportion of taxifolin pentaglutarate in the material, on the contrary, led to a significant increase in cell migration through the material. The results obtained indicated the possibility of modulating cell adhesion and migration in biomaterials by including various taxifolin derivatives in their composition. Thus, materials obtained on the basis of collagen and taxifolin derivatives may be of interest for regenerative medicine.

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ACKNOWLEDGMENTS

The authors thank V.G. Zaikin (CJSC “NPF “Flavit”) for the provided taxifolin. The work was carried out using the instrumental base of the CCU of ITEB RAS.

The work was supported by the Russian Science Foundation (project no. 23-25-00149).

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Correspondence to V. S. Shubina .

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Shatalin, Y.V., Kobyakova, M.I. & Shubina, V.S. Modulation of Adhesion and Migration of NIH/3T3 Cells in Collagen Materials by Taxifolin Derivatives. Biochem. Moscow Suppl. Ser. A 17 (Suppl 1), S85–S93 (2023). https://doi.org/10.1134/S1990747823070048

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Received : 13 September 2023

Revised : 20 September 2023

Accepted : 21 September 2023

Published : 17 January 2024

Issue Date : December 2023

DOI : https://doi.org/10.1134/S1990747823070048

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