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Priya ranganathan.
1 Department of Anesthesiology, Critical Care and Pain, Tata Memorial Hospital, Mumbai, Maharashtra, India
2 Department of Surgical Oncology, Tata Memorial Centre, Mumbai, Maharashtra, India
The second article in this series on biostatistics covers the concepts of sample, population, research hypotheses and statistical errors.
Ranganathan P, Pramesh CS. An Introduction to Statistics: Understanding Hypothesis Testing and Statistical Errors. Indian J Crit Care Med 2019;23(Suppl 3):S230–S231.
Two papers quoted in this issue of the Indian Journal of Critical Care Medicine report. The results of studies aim to prove that a new intervention is better than (superior to) an existing treatment. In the ABLE study, the investigators wanted to show that transfusion of fresh red blood cells would be superior to standard-issue red cells in reducing 90-day mortality in ICU patients. 1 The PROPPR study was designed to prove that transfusion of a lower ratio of plasma and platelets to red cells would be superior to a higher ratio in decreasing 24-hour and 30-day mortality in critically ill patients. 2 These studies are known as superiority studies (as opposed to noninferiority or equivalence studies which will be discussed in a subsequent article).
A sample represents a group of participants selected from the entire population. Since studies cannot be carried out on entire populations, researchers choose samples, which are representative of the population. This is similar to walking into a grocery store and examining a few grains of rice or wheat before purchasing an entire bag; we assume that the few grains that we select (the sample) are representative of the entire sack of grains (the population).
The results of the study are then extrapolated to generate inferences about the population. We do this using a process known as hypothesis testing. This means that the results of the study may not always be identical to the results we would expect to find in the population; i.e., there is the possibility that the study results may be erroneous.
A clinical trial begins with an assumption or belief, and then proceeds to either prove or disprove this assumption. In statistical terms, this belief or assumption is known as a hypothesis. Counterintuitively, what the researcher believes in (or is trying to prove) is called the “alternate” hypothesis, and the opposite is called the “null” hypothesis; every study has a null hypothesis and an alternate hypothesis. For superiority studies, the alternate hypothesis states that one treatment (usually the new or experimental treatment) is superior to the other; the null hypothesis states that there is no difference between the treatments (the treatments are equal). For example, in the ABLE study, we start by stating the null hypothesis—there is no difference in mortality between groups receiving fresh RBCs and standard-issue RBCs. We then state the alternate hypothesis—There is a difference between groups receiving fresh RBCs and standard-issue RBCs. It is important to note that we have stated that the groups are different, without specifying which group will be better than the other. This is known as a two-tailed hypothesis and it allows us to test for superiority on either side (using a two-sided test). This is because, when we start a study, we are not 100% certain that the new treatment can only be better than the standard treatment—it could be worse, and if it is so, the study should pick it up as well. One tailed hypothesis and one-sided statistical testing is done for non-inferiority studies, which will be discussed in a subsequent paper in this series.
There are two possibilities to consider when interpreting the results of a superiority study. The first possibility is that there is truly no difference between the treatments but the study finds that they are different. This is called a Type-1 error or false-positive error or alpha error. This means falsely rejecting the null hypothesis.
The second possibility is that there is a difference between the treatments and the study does not pick up this difference. This is called a Type 2 error or false-negative error or beta error. This means falsely accepting the null hypothesis.
The power of the study is the ability to detect a difference between groups and is the converse of the beta error; i.e., power = 1-beta error. Alpha and beta errors are finalized when the protocol is written and form the basis for sample size calculation for the study. In an ideal world, we would not like any error in the results of our study; however, we would need to do the study in the entire population (infinite sample size) to be able to get a 0% alpha and beta error. These two errors enable us to do studies with realistic sample sizes, with the compromise that there is a small possibility that the results may not always reflect the truth. The basis for this will be discussed in a subsequent paper in this series dealing with sample size calculation.
Conventionally, type 1 or alpha error is set at 5%. This means, that at the end of the study, if there is a difference between groups, we want to be 95% certain that this is a true difference and allow only a 5% probability that this difference has occurred by chance (false positive). Type 2 or beta error is usually set between 10% and 20%; therefore, the power of the study is 90% or 80%. This means that if there is a difference between groups, we want to be 80% (or 90%) certain that the study will detect that difference. For example, in the ABLE study, sample size was calculated with a type 1 error of 5% (two-sided) and power of 90% (type 2 error of 10%) (1).
Table 1 gives a summary of the two types of statistical errors with an example
Statistical errors
(a) Types of statistical errors | |||
: Null hypothesis is | |||
True | False | ||
Null hypothesis is actually | True | Correct results! | Falsely rejecting null hypothesis - Type I error |
False | Falsely accepting null hypothesis - Type II error | Correct results! | |
(b) Possible statistical errors in the ABLE trial | |||
There is difference in mortality between groups receiving fresh RBCs and standard-issue RBCs | There difference in mortality between groups receiving fresh RBCs and standard-issue RBCs | ||
Truth | There is difference in mortality between groups receiving fresh RBCs and standard-issue RBCs | Correct results! | Falsely rejecting null hypothesis - Type I error |
There difference in mortality between groups receiving fresh RBCs and standard-issue RBCs | Falsely accepting null hypothesis - Type II error | Correct results! |
In the next article in this series, we will look at the meaning and interpretation of ‘ p ’ value and confidence intervals for hypothesis testing.
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Conflict of interest: None
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A hypothesis test is a statistical inference method used to test the significance of a proposed (hypothesized) relation between population statistics (parameters) and their corresponding sample estimators . In other words, hypothesis tests are used to determine if there is enough evidence in a sample to prove a hypothesis true for the entire population.
The test considers two hypotheses: the null hypothesis , which is a statement meant to be tested, usually something like "there is no effect" with the intention of proving this false, and the alternate hypothesis , which is the statement meant to stand after the test is performed. The two hypotheses must be mutually exclusive ; moreover, in most applications, the two are complementary (one being the negation of the other). The test works by comparing the \(p\)-value to the level of significance (a chosen target). If the \(p\)-value is less than or equal to the level of significance, then the null hypothesis is rejected.
When analyzing data, only samples of a certain size might be manageable as efficient computations. In some situations the error terms follow a continuous or infinite distribution, hence the use of samples to suggest accuracy of the chosen test statistics. The method of hypothesis testing gives an advantage over guessing what distribution or which parameters the data follows.
Hypothesis test and confidence intervals.
In statistical inference, properties (parameters) of a population are analyzed by sampling data sets. Given assumptions on the distribution, i.e. a statistical model of the data, certain hypotheses can be deduced from the known behavior of the model. These hypotheses must be tested against sampled data from the population.
The null hypothesis \((\)denoted \(H_0)\) is a statement that is assumed to be true. If the null hypothesis is rejected, then there is enough evidence (statistical significance) to accept the alternate hypothesis \((\)denoted \(H_1).\) Before doing any test for significance, both hypotheses must be clearly stated and non-conflictive, i.e. mutually exclusive, statements. Rejecting the null hypothesis, given that it is true, is called a type I error and it is denoted \(\alpha\), which is also its probability of occurrence. Failing to reject the null hypothesis, given that it is false, is called a type II error and it is denoted \(\beta\), which is also its probability of occurrence. Also, \(\alpha\) is known as the significance level , and \(1-\beta\) is known as the power of the test. \(H_0\) \(\textbf{is true}\)\(\hspace{15mm}\) \(H_0\) \(\textbf{is false}\) \(\textbf{Reject}\) \(H_0\)\(\hspace{10mm}\) Type I error Correct Decision \(\textbf{Reject}\) \(H_1\) Correct Decision Type II error The test statistic is the standardized value following the sampled data under the assumption that the null hypothesis is true, and a chosen particular test. These tests depend on the statistic to be studied and the assumed distribution it follows, e.g. the population mean following a normal distribution. The \(p\)-value is the probability of observing an extreme test statistic in the direction of the alternate hypothesis, given that the null hypothesis is true. The critical value is the value of the assumed distribution of the test statistic such that the probability of making a type I error is small.
Methodologies: Given an estimator \(\hat \theta\) of a population statistic \(\theta\), following a probability distribution \(P(T)\), computed from a sample \(\mathcal{S},\) and given a significance level \(\alpha\) and test statistic \(t^*,\) define \(H_0\) and \(H_1;\) compute the test statistic \(t^*.\) \(p\)-value Approach (most prevalent): Find the \(p\)-value using \(t^*\) (right-tailed). If the \(p\)-value is at most \(\alpha,\) reject \(H_0\). Otherwise, reject \(H_1\). Critical Value Approach: Find the critical value solving the equation \(P(T\geq t_\alpha)=\alpha\) (right-tailed). If \(t^*>t_\alpha\), reject \(H_0\). Otherwise, reject \(H_1\). Note: Failing to reject \(H_0\) only means inability to accept \(H_1\), and it does not mean to accept \(H_0\).
Assume a normally distributed population has recorded cholesterol levels with various statistics computed. From a sample of 100 subjects in the population, the sample mean was 214.12 mg/dL (milligrams per deciliter), with a sample standard deviation of 45.71 mg/dL. Perform a hypothesis test, with significance level 0.05, to test if there is enough evidence to conclude that the population mean is larger than 200 mg/dL. Hypothesis Test We will perform a hypothesis test using the \(p\)-value approach with significance level \(\alpha=0.05:\) Define \(H_0\): \(\mu=200\). Define \(H_1\): \(\mu>200\). Since our values are normally distributed, the test statistic is \(z^*=\frac{\bar X - \mu_0}{\frac{s}{\sqrt{n}}}=\frac{214.12 - 200}{\frac{45.71}{\sqrt{100}}}\approx 3.09\). Using a standard normal distribution, we find that our \(p\)-value is approximately \(0.001\). Since the \(p\)-value is at most \(\alpha=0.05,\) we reject \(H_0\). Therefore, we can conclude that the test shows sufficient evidence to support the claim that \(\mu\) is larger than \(200\) mg/dL.
If the sample size was smaller, the normal and \(t\)-distributions behave differently. Also, the question itself must be managed by a double-tail test instead.
Assume a population's cholesterol levels are recorded and various statistics are computed. From a sample of 25 subjects, the sample mean was 214.12 mg/dL (milligrams per deciliter), with a sample standard deviation of 45.71 mg/dL. Perform a hypothesis test, with significance level 0.05, to test if there is enough evidence to conclude that the population mean is not equal to 200 mg/dL. Hypothesis Test We will perform a hypothesis test using the \(p\)-value approach with significance level \(\alpha=0.05\) and the \(t\)-distribution with 24 degrees of freedom: Define \(H_0\): \(\mu=200\). Define \(H_1\): \(\mu\neq 200\). Using the \(t\)-distribution, the test statistic is \(t^*=\frac{\bar X - \mu_0}{\frac{s}{\sqrt{n}}}=\frac{214.12 - 200}{\frac{45.71}{\sqrt{25}}}\approx 1.54\). Using a \(t\)-distribution with 24 degrees of freedom, we find that our \(p\)-value is approximately \(2(0.068)=0.136\). We have multiplied by two since this is a two-tailed argument, i.e. the mean can be smaller than or larger than. Since the \(p\)-value is larger than \(\alpha=0.05,\) we fail to reject \(H_0\). Therefore, the test does not show sufficient evidence to support the claim that \(\mu\) is not equal to \(200\) mg/dL.
The complement of the rejection on a two-tailed hypothesis test (with significance level \(\alpha\)) for a population parameter \(\theta\) is equivalent to finding a confidence interval \((\)with confidence level \(1-\alpha)\) for the population parameter \(\theta\). If the assumption on the parameter \(\theta\) falls inside the confidence interval, then the test has failed to reject the null hypothesis \((\)with \(p\)-value greater than \(\alpha).\) Otherwise, if \(\theta\) does not fall in the confidence interval, then the null hypothesis is rejected in favor of the alternate \((\)with \(p\)-value at most \(\alpha).\)
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S.3 hypothesis testing.
In reviewing hypothesis tests, we start first with the general idea. Then, we keep returning to the basic procedures of hypothesis testing, each time adding a little more detail.
The general idea of hypothesis testing involves:
Every hypothesis test — regardless of the population parameter involved — requires the above three steps.
Is normal body temperature really 98.6 degrees f section .
Consider the population of many, many adults. A researcher hypothesized that the average adult body temperature is lower than the often-advertised 98.6 degrees F. That is, the researcher wants an answer to the question: "Is the average adult body temperature 98.6 degrees? Or is it lower?" To answer his research question, the researcher starts by assuming that the average adult body temperature was 98.6 degrees F.
Then, the researcher went out and tried to find evidence that refutes his initial assumption. In doing so, he selects a random sample of 130 adults. The average body temperature of the 130 sampled adults is 98.25 degrees.
Then, the researcher uses the data he collected to make a decision about his initial assumption. It is either likely or unlikely that the researcher would collect the evidence he did given his initial assumption that the average adult body temperature is 98.6 degrees:
In statistics, we generally don't make claims that require us to believe that a very unusual event happened. That is, in the practice of statistics, if the evidence (data) we collected is unlikely in light of the initial assumption, then we reject our initial assumption.
Criminal trial analogy section .
One place where you can consistently see the general idea of hypothesis testing in action is in criminal trials held in the United States. Our criminal justice system assumes "the defendant is innocent until proven guilty." That is, our initial assumption is that the defendant is innocent.
In the practice of statistics, we make our initial assumption when we state our two competing hypotheses -- the null hypothesis ( H 0 ) and the alternative hypothesis ( H A ). Here, our hypotheses are:
In statistics, we always assume the null hypothesis is true . That is, the null hypothesis is always our initial assumption.
The prosecution team then collects evidence — such as finger prints, blood spots, hair samples, carpet fibers, shoe prints, ransom notes, and handwriting samples — with the hopes of finding "sufficient evidence" to make the assumption of innocence refutable.
In statistics, the data are the evidence.
The jury then makes a decision based on the available evidence:
In statistics, we always make one of two decisions. We either "reject the null hypothesis" or we "fail to reject the null hypothesis."
Did you notice the use of the phrase "behave as if" in the previous discussion? We "behave as if" the defendant is guilty; we do not "prove" that the defendant is guilty. And, we "behave as if" the defendant is innocent; we do not "prove" that the defendant is innocent.
This is a very important distinction! We make our decision based on evidence not on 100% guaranteed proof. Again:
We merely state that there is enough evidence to behave one way or the other. This is always true in statistics! Because of this, whatever the decision, there is always a chance that we made an error .
Let's review the two types of errors that can be made in criminal trials:
Jury Decision | Truth | ||
---|---|---|---|
Not Guilty | Guilty | ||
Not Guilty | OK | ERROR | |
Guilty | ERROR | OK |
Table S.3.2 shows how this corresponds to the two types of errors in hypothesis testing.
Decision | |||
---|---|---|---|
Null Hypothesis | Alternative Hypothesis | ||
Do not Reject Null | OK | Type II Error | |
Reject Null | Type I Error | OK |
Note that, in statistics, we call the two types of errors by two different names -- one is called a "Type I error," and the other is called a "Type II error." Here are the formal definitions of the two types of errors:
There is always a chance of making one of these errors. But, a good scientific study will minimize the chance of doing so!
Recall that it is either likely or unlikely that we would observe the evidence we did given our initial assumption. If it is likely , we do not reject the null hypothesis. If it is unlikely , then we reject the null hypothesis in favor of the alternative hypothesis. Effectively, then, making the decision reduces to determining "likely" or "unlikely."
In statistics, there are two ways to determine whether the evidence is likely or unlikely given the initial assumption:
In the next two sections, we review the procedures behind each of these two approaches. To make our review concrete, let's imagine that μ is the average grade point average of all American students who major in mathematics. We first review the critical value approach for conducting each of the following three hypothesis tests about the population mean $\mu$:
: = 3 | : > 3 | |
: = 3 | : < 3 | |
: = 3 | : ≠ 3 |
Upon completing the review of the critical value approach, we review the P -value approach for conducting each of the above three hypothesis tests about the population mean \(\mu\). The procedures that we review here for both approaches easily extend to hypothesis tests about any other population parameter.
Hypothesis testing is a tool for making statistical inferences about the population data. It is an analysis tool that tests assumptions and determines how likely something is within a given standard of accuracy. Hypothesis testing provides a way to verify whether the results of an experiment are valid.
A null hypothesis and an alternative hypothesis are set up before performing the hypothesis testing. This helps to arrive at a conclusion regarding the sample obtained from the population. In this article, we will learn more about hypothesis testing, its types, steps to perform the testing, and associated examples.
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Hypothesis testing uses sample data from the population to draw useful conclusions regarding the population probability distribution . It tests an assumption made about the data using different types of hypothesis testing methodologies. The hypothesis testing results in either rejecting or not rejecting the null hypothesis.
Hypothesis testing can be defined as a statistical tool that is used to identify if the results of an experiment are meaningful or not. It involves setting up a null hypothesis and an alternative hypothesis. These two hypotheses will always be mutually exclusive. This means that if the null hypothesis is true then the alternative hypothesis is false and vice versa. An example of hypothesis testing is setting up a test to check if a new medicine works on a disease in a more efficient manner.
The null hypothesis is a concise mathematical statement that is used to indicate that there is no difference between two possibilities. In other words, there is no difference between certain characteristics of data. This hypothesis assumes that the outcomes of an experiment are based on chance alone. It is denoted as \(H_{0}\). Hypothesis testing is used to conclude if the null hypothesis can be rejected or not. Suppose an experiment is conducted to check if girls are shorter than boys at the age of 5. The null hypothesis will say that they are the same height.
The alternative hypothesis is an alternative to the null hypothesis. It is used to show that the observations of an experiment are due to some real effect. It indicates that there is a statistical significance between two possible outcomes and can be denoted as \(H_{1}\) or \(H_{a}\). For the above-mentioned example, the alternative hypothesis would be that girls are shorter than boys at the age of 5.
In hypothesis testing, the p value is used to indicate whether the results obtained after conducting a test are statistically significant or not. It also indicates the probability of making an error in rejecting or not rejecting the null hypothesis.This value is always a number between 0 and 1. The p value is compared to an alpha level, \(\alpha\) or significance level. The alpha level can be defined as the acceptable risk of incorrectly rejecting the null hypothesis. The alpha level is usually chosen between 1% to 5%.
All sets of values that lead to rejecting the null hypothesis lie in the critical region. Furthermore, the value that separates the critical region from the non-critical region is known as the critical value.
Depending upon the type of data available and the size, different types of hypothesis testing are used to determine whether the null hypothesis can be rejected or not. The hypothesis testing formula for some important test statistics are given below:
We will learn more about these test statistics in the upcoming section.
Selecting the correct test for performing hypothesis testing can be confusing. These tests are used to determine a test statistic on the basis of which the null hypothesis can either be rejected or not rejected. Some of the important tests used for hypothesis testing are given below.
A z test is a way of hypothesis testing that is used for a large sample size (n ≥ 30). It is used to determine whether there is a difference between the population mean and the sample mean when the population standard deviation is known. It can also be used to compare the mean of two samples. It is used to compute the z test statistic. The formulas are given as follows:
The t test is another method of hypothesis testing that is used for a small sample size (n < 30). It is also used to compare the sample mean and population mean. However, the population standard deviation is not known. Instead, the sample standard deviation is known. The mean of two samples can also be compared using the t test.
The Chi square test is a hypothesis testing method that is used to check whether the variables in a population are independent or not. It is used when the test statistic is chi-squared distributed.
One tailed hypothesis testing is done when the rejection region is only in one direction. It can also be known as directional hypothesis testing because the effects can be tested in one direction only. This type of testing is further classified into the right tailed test and left tailed test.
Right Tailed Hypothesis Testing
The right tail test is also known as the upper tail test. This test is used to check whether the population parameter is greater than some value. The null and alternative hypotheses for this test are given as follows:
\(H_{0}\): The population parameter is ≤ some value
\(H_{1}\): The population parameter is > some value.
If the test statistic has a greater value than the critical value then the null hypothesis is rejected
Left Tailed Hypothesis Testing
The left tail test is also known as the lower tail test. It is used to check whether the population parameter is less than some value. The hypotheses for this hypothesis testing can be written as follows:
\(H_{0}\): The population parameter is ≥ some value
\(H_{1}\): The population parameter is < some value.
The null hypothesis is rejected if the test statistic has a value lesser than the critical value.
In this hypothesis testing method, the critical region lies on both sides of the sampling distribution. It is also known as a non - directional hypothesis testing method. The two-tailed test is used when it needs to be determined if the population parameter is assumed to be different than some value. The hypotheses can be set up as follows:
\(H_{0}\): the population parameter = some value
\(H_{1}\): the population parameter ≠ some value
The null hypothesis is rejected if the test statistic has a value that is not equal to the critical value.
Hypothesis testing can be easily performed in five simple steps. The most important step is to correctly set up the hypotheses and identify the right method for hypothesis testing. The basic steps to perform hypothesis testing are as follows:
The best way to solve a problem on hypothesis testing is by applying the 5 steps mentioned in the previous section. Suppose a researcher claims that the mean average weight of men is greater than 100kgs with a standard deviation of 15kgs. 30 men are chosen with an average weight of 112.5 Kgs. Using hypothesis testing, check if there is enough evidence to support the researcher's claim. The confidence interval is given as 95%.
Step 1: This is an example of a right-tailed test. Set up the null hypothesis as \(H_{0}\): \(\mu\) = 100.
Step 2: The alternative hypothesis is given by \(H_{1}\): \(\mu\) > 100.
Step 3: As this is a one-tailed test, \(\alpha\) = 100% - 95% = 5%. This can be used to determine the critical value.
1 - \(\alpha\) = 1 - 0.05 = 0.95
0.95 gives the required area under the curve. Now using a normal distribution table, the area 0.95 is at z = 1.645. A similar process can be followed for a t-test. The only additional requirement is to calculate the degrees of freedom given by n - 1.
Step 4: Calculate the z test statistic. This is because the sample size is 30. Furthermore, the sample and population means are known along with the standard deviation.
z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\).
\(\mu\) = 100, \(\overline{x}\) = 112.5, n = 30, \(\sigma\) = 15
z = \(\frac{112.5-100}{\frac{15}{\sqrt{30}}}\) = 4.56
Step 5: Conclusion. As 4.56 > 1.645 thus, the null hypothesis can be rejected.
Confidence intervals form an important part of hypothesis testing. This is because the alpha level can be determined from a given confidence interval. Suppose a confidence interval is given as 95%. Subtract the confidence interval from 100%. This gives 100 - 95 = 5% or 0.05. This is the alpha value of a one-tailed hypothesis testing. To obtain the alpha value for a two-tailed hypothesis testing, divide this value by 2. This gives 0.05 / 2 = 0.025.
Related Articles:
Important Notes on Hypothesis Testing
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What is hypothesis testing.
Hypothesis testing in statistics is a tool that is used to make inferences about the population data. It is also used to check if the results of an experiment are valid.
The z test in hypothesis testing is used to find the z test statistic for normally distributed data . The z test is used when the standard deviation of the population is known and the sample size is greater than or equal to 30.
The t test in hypothesis testing is used when the data follows a student t distribution . It is used when the sample size is less than 30 and standard deviation of the population is not known.
The formula for a one sample z test in hypothesis testing is z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\) and for two samples is z = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}\).
The p value helps to determine if the test results are statistically significant or not. In hypothesis testing, the null hypothesis can either be rejected or not rejected based on the comparison between the p value and the alpha level.
When the rejection region is only on one side of the distribution curve then it is known as one tail hypothesis testing. The right tail test and the left tail test are two types of directional hypothesis testing.
To get the alpha level in a two tail hypothesis testing divide \(\alpha\) by 2. This is done as there are two rejection regions in the curve.
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Published on May 6, 2022 by Shaun Turney . Revised on June 22, 2023.
The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :
Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, similarities and differences between null and alternative hypotheses, how to write null and alternative hypotheses, other interesting articles, frequently asked questions.
The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”:
The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample. It’s critical for your research to write strong hypotheses .
You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.
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The null hypothesis is the claim that there’s no effect in the population.
If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.
Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept . Be careful not to say you “prove” or “accept” the null hypothesis.
Null hypotheses often include phrases such as “no effect,” “no difference,” or “no relationship.” When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).
You can never know with complete certainty whether there is an effect in the population. Some percentage of the time, your inference about the population will be incorrect. When you incorrectly reject the null hypothesis, it’s called a type I error . When you incorrectly fail to reject it, it’s a type II error.
The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.
( ) | ||
Does tooth flossing affect the number of cavities? | Tooth flossing has on the number of cavities. | test: The mean number of cavities per person does not differ between the flossing group (µ ) and the non-flossing group (µ ) in the population; µ = µ . |
Does the amount of text highlighted in the textbook affect exam scores? | The amount of text highlighted in the textbook has on exam scores. | : There is no relationship between the amount of text highlighted and exam scores in the population; β = 0. |
Does daily meditation decrease the incidence of depression? | Daily meditation the incidence of depression.* | test: The proportion of people with depression in the daily-meditation group ( ) is greater than or equal to the no-meditation group ( ) in the population; ≥ . |
*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .
The alternative hypothesis ( H a ) is the other answer to your research question . It claims that there’s an effect in the population.
Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.
The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.
Alternative hypotheses often include phrases such as “an effect,” “a difference,” or “a relationship.” When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes < or >). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.
The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.
Does tooth flossing affect the number of cavities? | Tooth flossing has an on the number of cavities. | test: The mean number of cavities per person differs between the flossing group (µ ) and the non-flossing group (µ ) in the population; µ ≠ µ . |
Does the amount of text highlighted in a textbook affect exam scores? | The amount of text highlighted in the textbook has an on exam scores. | : There is a relationship between the amount of text highlighted and exam scores in the population; β ≠ 0. |
Does daily meditation decrease the incidence of depression? | Daily meditation the incidence of depression. | test: The proportion of people with depression in the daily-meditation group ( ) is less than the no-meditation group ( ) in the population; < . |
Null and alternative hypotheses are similar in some ways:
However, there are important differences between the two types of hypotheses, summarized in the following table.
A claim that there is in the population. | A claim that there is in the population. | |
| ||
Equality symbol (=, ≥, or ≤) | Inequality symbol (≠, <, or >) | |
Rejected | Supported | |
Failed to reject | Not supported |
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To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.
The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:
Does independent variable affect dependent variable ?
Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.
( ) | ||
test
with two groups | The mean dependent variable does not differ between group 1 (µ ) and group 2 (µ ) in the population; µ = µ . | The mean dependent variable differs between group 1 (µ ) and group 2 (µ ) in the population; µ ≠ µ . |
with three groups | The mean dependent variable does not differ between group 1 (µ ), group 2 (µ ), and group 3 (µ ) in the population; µ = µ = µ . | The mean dependent variable of group 1 (µ ), group 2 (µ ), and group 3 (µ ) are not all equal in the population. |
There is no correlation between independent variable and dependent variable in the population; ρ = 0. | There is a correlation between independent variable and dependent variable in the population; ρ ≠ 0. | |
There is no relationship between independent variable and dependent variable in the population; β = 0. | There is a relationship between independent variable and dependent variable in the population; β ≠ 0. | |
Two-proportions test | The dependent variable expressed as a proportion does not differ between group 1 ( ) and group 2 ( ) in the population; = . | The dependent variable expressed as a proportion differs between group 1 ( ) and group 2 ( ) in the population; ≠ . |
Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.
If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.
Methodology
Research bias
Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.
Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.
The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).
The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).
A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (“ x affects y because …”).
A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses . In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.
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Bayesian methods.
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Hypothesis testing is a form of statistical inference that uses data from a sample to draw conclusions about a population parameter or a population probability distribution . First, a tentative assumption is made about the parameter or distribution. This assumption is called the null hypothesis and is denoted by H 0 . An alternative hypothesis (denoted H a ), which is the opposite of what is stated in the null hypothesis, is then defined. The hypothesis-testing procedure involves using sample data to determine whether or not H 0 can be rejected. If H 0 is rejected, the statistical conclusion is that the alternative hypothesis H a is true.
For example, assume that a radio station selects the music it plays based on the assumption that the average age of its listening audience is 30 years. To determine whether this assumption is valid, a hypothesis test could be conducted with the null hypothesis given as H 0 : μ = 30 and the alternative hypothesis given as H a : μ ≠ 30. Based on a sample of individuals from the listening audience, the sample mean age, x̄ , can be computed and used to determine whether there is sufficient statistical evidence to reject H 0 . Conceptually, a value of the sample mean that is “close” to 30 is consistent with the null hypothesis, while a value of the sample mean that is “not close” to 30 provides support for the alternative hypothesis. What is considered “close” and “not close” is determined by using the sampling distribution of x̄ .
Ideally, the hypothesis-testing procedure leads to the acceptance of H 0 when H 0 is true and the rejection of H 0 when H 0 is false. Unfortunately, since hypothesis tests are based on sample information, the possibility of errors must be considered. A type I error corresponds to rejecting H 0 when H 0 is actually true, and a type II error corresponds to accepting H 0 when H 0 is false. The probability of making a type I error is denoted by α, and the probability of making a type II error is denoted by β.
In using the hypothesis-testing procedure to determine if the null hypothesis should be rejected, the person conducting the hypothesis test specifies the maximum allowable probability of making a type I error, called the level of significance for the test. Common choices for the level of significance are α = 0.05 and α = 0.01. Although most applications of hypothesis testing control the probability of making a type I error, they do not always control the probability of making a type II error. A graph known as an operating-characteristic curve can be constructed to show how changes in the sample size affect the probability of making a type II error.
A concept known as the p -value provides a convenient basis for drawing conclusions in hypothesis-testing applications. The p -value is a measure of how likely the sample results are, assuming the null hypothesis is true; the smaller the p -value, the less likely the sample results. If the p -value is less than α, the null hypothesis can be rejected; otherwise, the null hypothesis cannot be rejected. The p -value is often called the observed level of significance for the test.
A hypothesis test can be performed on parameters of one or more populations as well as in a variety of other situations. In each instance, the process begins with the formulation of null and alternative hypotheses about the population. In addition to the population mean, hypothesis-testing procedures are available for population parameters such as proportions, variances , standard deviations , and medians .
Hypothesis tests are also conducted in regression and correlation analysis to determine if the regression relationship and the correlation coefficient are statistically significant (see below Regression and correlation analysis ). A goodness-of-fit test refers to a hypothesis test in which the null hypothesis is that the population has a specific probability distribution, such as a normal probability distribution. Nonparametric statistical methods also involve a variety of hypothesis-testing procedures.
The methods of statistical inference previously described are often referred to as classical methods. Bayesian methods (so called after the English mathematician Thomas Bayes ) provide alternatives that allow one to combine prior information about a population parameter with information contained in a sample to guide the statistical inference process. A prior probability distribution for a parameter of interest is specified first. Sample information is then obtained and combined through an application of Bayes’s theorem to provide a posterior probability distribution for the parameter. The posterior distribution provides the basis for statistical inferences concerning the parameter.
A key, and somewhat controversial, feature of Bayesian methods is the notion of a probability distribution for a population parameter. According to classical statistics, parameters are constants and cannot be represented as random variables. Bayesian proponents argue that, if a parameter value is unknown, then it makes sense to specify a probability distribution that describes the possible values for the parameter as well as their likelihood . The Bayesian approach permits the use of objective data or subjective opinion in specifying a prior distribution. With the Bayesian approach, different individuals might specify different prior distributions. Classical statisticians argue that for this reason Bayesian methods suffer from a lack of objectivity. Bayesian proponents argue that the classical methods of statistical inference have built-in subjectivity (through the choice of a sampling plan) and that the advantage of the Bayesian approach is that the subjectivity is made explicit.
Bayesian methods have been used extensively in statistical decision theory (see below Decision analysis ). In this context , Bayes’s theorem provides a mechanism for combining a prior probability distribution for the states of nature with sample information to provide a revised (posterior) probability distribution about the states of nature. These posterior probabilities are then used to make better decisions.
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Jenna Lehmann
Hypothesis testing is a big part of what we would actually consider testing for inferential statistics. It’s a procedure and set of rules that allow us to move from descriptive statistics to make inferences about a population based on sample data. It is a statistical method that uses sample data to evaluate a hypothesis about a population.
This type of test is usually used within the context of research. If we expect to see a difference between a treated and untreated group (in some cases the untreated group is the parameters we know about the population), we expect there to be a difference in the means between the two groups, but that the standard deviation remains the same, as if each individual score has had a value added or subtracted from it.
The following steps will be tailored to fit the first kind of hypothesis testing we will learn first: single-sample z-tests. There are many other kinds of tests, so keep this in mind.
Because we’re making judgments based on probability and proportion, our normal distributions and certain regions within them come into play.
The Critical Region is composed of the extreme sample values that are very unlikely to be obtained if the null hypothesis is true. Determined by alpha level. If sample data fall in the critical region, the null hypothesis is rejected, because it’s very unlikely they’ve fallen there by chance.
These regions come into play when talking about different errors.
A Type I Error occurs when a researcher rejects a null hypothesis that is actually true; the researcher concludes that a treatment has an effect when it actually doesn’t. This happens when a researcher unknowingly obtains an extreme, non-representative sample. This goes back to alpha level: it’s the probability that the test will lead to a Type I error if the null hypothesis is true.
A result is said to be significant or statistically significant if it is very unlikely to occur when the null hypothesis is true. That is, the result is sufficient to reject the null hypothesis. For instance, two means can be significantly different from one another.
Assumptions of Hypothesis Testing:
Factors that influence hypothesis testing:
Test statistic: indicates that the sample data are converted into a single, specific statistic that is used to test the hypothesis (in this case, the z-score statistic).
In a directional hypothesis test , also known as a one-tailed test, the statistical hypotheses specify with an increase or decrease in the population mean. That is, they make a statement about the direction of the effect.
The Hypotheses for a Directional Test:
Because we’re only worried about scores that are either greater or less than the scores predicted by the null hypothesis, we only worry about what’s going on in one tail meaning that the critical region only exists within one tail. This means that all of the alpha is contained in one tail rather than split up into both (so the whole 5% is located in the tail we care about, rather than 2.5% in each tail). So before, we cared about what’s going on at the 0.025 mark of the unit normal table to look at both tails, but now we care about 0.05 because we’re only looking at one tail.
A one-tailed test allows you to reject the null hypothesis when the difference between the sample and the population is relatively small, as long as that difference is in the direction that you predicted. A two-tailed test, on the other hand, requires a relatively large difference independent of direction. In practice, researchers hypothesize using a one-tailed method but base their findings off of whether the results fall into the critical region of a two-tailed method. For the purposes of this class, make sure to calculate your results using the test that is specified in the problem.
A measure of effect size is intended to provide a measurement of the absolute magnitude of a treatment effect, independent of the size of the sample(s) being used. Usually done with Cohen’s d. If you imagine the two distributions, they’re layered over one another. The more they overlap, the smaller the effect size (the means of the two distributions are close). The more they are spread apart, the greater the effect size (the means of the two distributions are farther apart).
The power of a statistical test is the probability that the test will correctly reject a false null hypothesis. It’s usually what we’re hoping to get when we run an experiment. It’s displayed in the table posted above. Power and effect size are connected. So, we know that the greater the distance between the means, the greater the effect size. If the two distributions overlapped very little, there would be a greater chance of selecting a sample that leads to rejecting the null hypothesis.
This chapter was originally posted to the Math Support Center blog at the University of Baltimore on June 11, 2019.
Math and Statistics Guides from UB's Math & Statistics Center Copyright © by Jenna Lehmann is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.
Teach yourself statistics
A statistical hypothesis is an assumption about a population parameter . This assumption may or may not be true. Hypothesis testing refers to the formal procedures used by statisticians to accept or reject statistical hypotheses.
The best way to determine whether a statistical hypothesis is true would be to examine the entire population. Since that is often impractical, researchers typically examine a random sample from the population. If sample data are not consistent with the statistical hypothesis, the hypothesis is rejected.
There are two types of statistical hypotheses.
For example, suppose we wanted to determine whether a coin was fair and balanced. A null hypothesis might be that half the flips would result in Heads and half, in Tails. The alternative hypothesis might be that the number of Heads and Tails would be very different. Symbolically, these hypotheses would be expressed as
H o : P = 0.5 H a : P ≠ 0.5
Suppose we flipped the coin 50 times, resulting in 40 Heads and 10 Tails. Given this result, we would be inclined to reject the null hypothesis. We would conclude, based on the evidence, that the coin was probably not fair and balanced.
Some researchers say that a hypothesis test can have one of two outcomes: you accept the null hypothesis or you reject the null hypothesis. Many statisticians, however, take issue with the notion of "accepting the null hypothesis." Instead, they say: you reject the null hypothesis or you fail to reject the null hypothesis.
Why the distinction between "acceptance" and "failure to reject?" Acceptance implies that the null hypothesis is true. Failure to reject implies that the data are not sufficiently persuasive for us to prefer the alternative hypothesis over the null hypothesis.
Statisticians follow a formal process to determine whether to reject a null hypothesis, based on sample data. This process, called hypothesis testing , consists of four steps.
Two types of errors can result from a hypothesis test.
The analysis plan for a hypothesis test must include decision rules for rejecting the null hypothesis. In practice, statisticians describe these decision rules in two ways - with reference to a P-value or with reference to a region of acceptance.
The set of values outside the region of acceptance is called the region of rejection . If the test statistic falls within the region of rejection, the null hypothesis is rejected. In such cases, we say that the hypothesis has been rejected at the α level of significance.
These approaches are equivalent. Some statistics texts use the P-value approach; others use the region of acceptance approach.
A test of a statistical hypothesis, where the region of rejection is on only one side of the sampling distribution , is called a one-tailed test . For example, suppose the null hypothesis states that the mean is less than or equal to 10. The alternative hypothesis would be that the mean is greater than 10. The region of rejection would consist of a range of numbers located on the right side of sampling distribution; that is, a set of numbers greater than 10.
A test of a statistical hypothesis, where the region of rejection is on both sides of the sampling distribution, is called a two-tailed test . For example, suppose the null hypothesis states that the mean is equal to 10. The alternative hypothesis would be that the mean is less than 10 or greater than 10. The region of rejection would consist of a range of numbers located on both sides of sampling distribution; that is, the region of rejection would consist partly of numbers that were less than 10 and partly of numbers that were greater than 10.
Topics: Hypothesis Testing , Data Analysis , Statistics
Hypothesis testing is an essential procedure in statistics. A hypothesis test evaluates two mutually exclusive statements about a population to determine which statement is best supported by the sample data. When we say that a finding is statistically significant, it’s thanks to a hypothesis test. How do these tests really work and what does statistical significance actually mean?
In this series of three posts, I’ll help you intuitively understand how hypothesis tests work by focusing on concepts and graphs rather than equations and numbers. After all, a key reason to use statistical software like Minitab is so you don’t get bogged down in the calculations and can instead focus on understanding your results.
To kick things off in this post, I highlight the rationale for using hypothesis tests with an example.
An economist wants to determine whether the monthly energy cost for families has changed from the previous year, when the mean cost per month was $260. The economist randomly samples 25 families and records their energy costs for the current year. (The data for this example is FamilyEnergyCost and it is just one of the many data set examples that can be found in Minitab’s Data Set Library.)
I’ll use these descriptive statistics to create a probability distribution plot that shows you the importance of hypothesis tests. Read on!
Why do we even need hypothesis tests? After all, we took a random sample and our sample mean of 330.6 is different from 260. That is different, right? Unfortunately, the picture is muddied because we’re looking at a sample rather than the entire population.
Sampling error is the difference between a sample and the entire population. Thanks to sampling error, it’s entirely possible that while our sample mean is 330.6, the population mean could still be 260. Or, to put it another way, if we repeated the experiment, it’s possible that the second sample mean could be close to 260. A hypothesis test helps assess the likelihood of this possibility!
For any given random sample, the mean of the sample almost certainly doesn’t equal the true mean of the population due to sampling error. For our example, it’s unlikely that the mean cost for the entire population is exactly 330.6. In fact, if we took multiple random samples of the same size from the same population, we could plot a distribution of the sample means.
A sampling distribution is the distribution of a statistic, such as the mean, that is obtained by repeatedly drawing a large number of samples from a specific population. This distribution allows you to determine the probability of obtaining the sample statistic.
Fortunately, I can create a plot of sample means without collecting many different random samples! Instead, I’ll create a probability distribution plot using the t-distribution , the sample size, and the variability in our sample to graph the sampling distribution.
Our goal is to determine whether our sample mean is significantly different from the null hypothesis mean. Therefore, we’ll use the graph to see whether our sample mean of 330.6 is unlikely assuming that the population mean is 260. The graph below shows the expected distribution of sample means.
You can see that the most probable sample mean is 260, which makes sense because we’re assuming that the null hypothesis is true. However, there is a reasonable probability of obtaining a sample mean that ranges from 167 to 352, and even beyond! The takeaway from this graph is that while our sample mean of 330.6 is not the most probable, it’s also not outside the realm of possibility.
We’ve placed our sample mean in the context of all possible sample means while assuming that the null hypothesis is true. Are these results statistically significant?
As you can see, there is no magic place on the distribution curve to make this determination. Instead, we have a continual decrease in the probability of obtaining sample means that are further from the null hypothesis value. Where do we draw the line?
This is where hypothesis tests are useful. A hypothesis test allows us quantify the probability that our sample mean is unusual.
For this series of posts, I’ll continue to use this graphical framework and add in the significance level, P value, and confidence interval to show how hypothesis tests work and what statistical significance really means.
If you'd like to see how I made these graphs, please read: How to Create a Graphical Version of the 1-sample t-Test .
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About hypothesis testing.
Watch the video for a brief overview of hypothesis testing:
Can’t see the video? Click here to watch it on YouTube.
Contents (Click to skip to the section):
What is hypothesis testing.
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A hypothesis is an educated guess about something in the world around you. It should be testable, either by experiment or observation. For example:
It can really be anything at all as long as you can put it to the test.
If you are going to propose a hypothesis, it’s customary to write a statement. Your statement will look like this: “If I…(do this to an independent variable )….then (this will happen to the dependent variable ).” For example:
A good hypothesis statement should:
Hypothesis testing can be one of the most confusing aspects for students, mostly because before you can even perform a test, you have to know what your null hypothesis is. Often, those tricky word problems that you are faced with can be difficult to decipher. But it’s easier than you think; all you need to do is:
If you trace back the history of science, the null hypothesis is always the accepted fact. Simple examples of null hypotheses that are generally accepted as being true are:
You won’t be required to actually perform a real experiment or survey in elementary statistics (or even disprove a fact like “Pluto is a planet”!), so you’ll be given word problems from real-life situations. You’ll need to figure out what your hypothesis is from the problem. This can be a little trickier than just figuring out what the accepted fact is. With word problems, you are looking to find a fact that is nullifiable (i.e. something you can reject).
A researcher thinks that if knee surgery patients go to physical therapy twice a week (instead of 3 times), their recovery period will be longer. Average recovery times for knee surgery patients is 8.2 weeks.
The hypothesis statement in this question is that the researcher believes the average recovery time is more than 8.2 weeks. It can be written in mathematical terms as: H 1 : μ > 8.2
Next, you’ll need to state the null hypothesis . That’s what will happen if the researcher is wrong . In the above example, if the researcher is wrong then the recovery time is less than or equal to 8.2 weeks. In math, that’s: H 0 μ ≤ 8.2
Ten or so years ago, we believed that there were 9 planets in the solar system. Pluto was demoted as a planet in 2006. The null hypothesis of “Pluto is a planet” was replaced by “Pluto is not a planet.” Of course, rejecting the null hypothesis isn’t always that easy— the hard part is usually figuring out what your null hypothesis is in the first place.
The one sample z test isn’t used very often (because we rarely know the actual population standard deviation ). However, it’s a good idea to understand how it works as it’s one of the simplest tests you can perform in hypothesis testing. In English class you got to learn the basics (like grammar and spelling) before you could write a story; think of one sample z tests as the foundation for understanding more complex hypothesis testing. This page contains two hypothesis testing examples for one sample z-tests .
Watch the video for an example:
A principal at a certain school claims that the students in his school are above average intelligence. A random sample of thirty students IQ scores have a mean score of 112.5. Is there sufficient evidence to support the principal’s claim? The mean population IQ is 100 with a standard deviation of 15.
Step 1: State the Null hypothesis . The accepted fact is that the population mean is 100, so: H 0 : μ = 100.
Step 2: State the Alternate Hypothesis . The claim is that the students have above average IQ scores, so: H 1 : μ > 100. The fact that we are looking for scores “greater than” a certain point means that this is a one-tailed test.
Step 4: State the alpha level . If you aren’t given an alpha level , use 5% (0.05).
Step 5: Find the rejection region area (given by your alpha level above) from the z-table . An area of .05 is equal to a z-score of 1.645.
Step 6: If Step 6 is greater than Step 5, reject the null hypothesis. If it’s less than Step 5, you cannot reject the null hypothesis. In this case, it is more (4.56 > 1.645), so you can reject the null.
Watch the video for an example of a two-tailed z-test:
Blood glucose levels for obese patients have a mean of 100 with a standard deviation of 15. A researcher thinks that a diet high in raw cornstarch will have a positive or negative effect on blood glucose levels. A sample of 30 patients who have tried the raw cornstarch diet have a mean glucose level of 140. Test the hypothesis that the raw cornstarch had an effect.
*This process is made much easier if you use a TI-83 or Excel to calculate the z-score (the “critical value”). See:
You can use the TI 83 calculator for hypothesis testing, but the calculator won’t figure out the null and alternate hypotheses; that’s up to you to read the question and input it into the calculator.
Example problem : A sample of 200 people has a mean age of 21 with a population standard deviation (σ) of 5. Test the hypothesis that the population mean is 18.9 at α = 0.05.
Step 1: State the null hypothesis. In this case, the null hypothesis is that the population mean is 18.9, so we write: H 0 : μ = 18.9
Step 2: State the alternative hypothesis. We want to know if our sample, which has a mean of 21 instead of 18.9, really is different from the population, therefore our alternate hypothesis: H 1 : μ ≠ 18.9
Step 3: Press Stat then press the right arrow twice to select TESTS.
Step 4: Press 1 to select 1:Z-Test… . Press ENTER.
Step 5: Use the right arrow to select Stats .
Step 6: Enter the data from the problem: μ 0 : 18.9 σ: 5 x : 21 n: 200 μ: ≠μ 0
Step 7: Arrow down to Calculate and press ENTER. The calculator shows the p-value: p = 2.87 × 10 -9
This is smaller than our alpha value of .05. That means we should reject the null hypothesis .
Bayesian hypothesis testing helps to answer the question: Can the results from a test or survey be repeated? Why do we care if a test can be repeated? Let’s say twenty people in the same village came down with leukemia. A group of researchers find that cell-phone towers are to blame. However, a second study found that cell-phone towers had nothing to do with the cancer cluster in the village. In fact, they found that the cancers were completely random. If that sounds impossible, it actually can happen! Clusters of cancer can happen simply by chance . There could be many reasons why the first study was faulty. One of the main reasons could be that they just didn’t take into account that sometimes things happen randomly and we just don’t know why.
It’s good science to let people know if your study results are solid, or if they could have happened by chance. The usual way of doing this is to test your results with a p-value . A p value is a number that you get by running a hypothesis test on your data. A P value of 0.05 (5%) or less is usually enough to claim that your results are repeatable. However, there’s another way to test the validity of your results: Bayesian Hypothesis testing. This type of testing gives you another way to test the strength of your results.
Traditional testing (the type you probably came across in elementary stats or AP stats) is called Non-Bayesian. It is how often an outcome happens over repeated runs of the experiment. It’s an objective view of whether an experiment is repeatable. Bayesian hypothesis testing is a subjective view of the same thing. It takes into account how much faith you have in your results. In other words, would you wager money on the outcome of your experiment?
Traditional testing (Non Bayesian) requires you to repeat sampling over and over, while Bayesian testing does not. The main different between the two is in the first step of testing: stating a probability model. In Bayesian testing you add prior knowledge to this step. It also requires use of a posterior probability , which is the conditional probability given to a random event after all the evidence is considered.
Many researchers think that it is a better alternative to traditional testing, because it:
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The bottom line.
Hypothesis testing, sometimes called significance testing, is an act in statistics whereby an analyst tests an assumption regarding a population parameter. The methodology employed by the analyst depends on the nature of the data used and the reason for the analysis.
Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data. Such data may come from a larger population or a data-generating process. The word "population" will be used for both of these cases in the following descriptions.
In hypothesis testing, an analyst tests a statistical sample, intending to provide evidence on the plausibility of the null hypothesis. Statistical analysts measure and examine a random sample of the population being analyzed. All analysts use a random population sample to test two different hypotheses: the null hypothesis and the alternative hypothesis.
The null hypothesis is usually a hypothesis of equality between population parameters; e.g., a null hypothesis may state that the population mean return is equal to zero. The alternative hypothesis is effectively the opposite of a null hypothesis. Thus, they are mutually exclusive , and only one can be true. However, one of the two hypotheses will always be true.
The null hypothesis is a statement about a population parameter, such as the population mean, that is assumed to be true.
If an individual wants to test that a penny has exactly a 50% chance of landing on heads, the null hypothesis would be that 50% is correct, and the alternative hypothesis would be that 50% is not correct. Mathematically, the null hypothesis is represented as Ho: P = 0.5. The alternative hypothesis is shown as "Ha" and is identical to the null hypothesis, except with the equal sign struck-through, meaning that it does not equal 50%.
A random sample of 100 coin flips is taken, and the null hypothesis is tested. If it is found that the 100 coin flips were distributed as 40 heads and 60 tails, the analyst would assume that a penny does not have a 50% chance of landing on heads and would reject the null hypothesis and accept the alternative hypothesis.
If there were 48 heads and 52 tails, then it is plausible that the coin could be fair and still produce such a result. In cases such as this where the null hypothesis is "accepted," the analyst states that the difference between the expected results (50 heads and 50 tails) and the observed results (48 heads and 52 tails) is "explainable by chance alone."
Some statisticians attribute the first hypothesis tests to satirical writer John Arbuthnot in 1710, who studied male and female births in England after observing that in nearly every year, male births exceeded female births by a slight proportion. Arbuthnot calculated that the probability of this happening by chance was small, and therefore it was due to “divine providence.”
Hypothesis testing helps assess the accuracy of new ideas or theories by testing them against data. This allows researchers to determine whether the evidence supports their hypothesis, helping to avoid false claims and conclusions. Hypothesis testing also provides a framework for decision-making based on data rather than personal opinions or biases. By relying on statistical analysis, hypothesis testing helps to reduce the effects of chance and confounding variables, providing a robust framework for making informed conclusions.
Hypothesis testing relies exclusively on data and doesn’t provide a comprehensive understanding of the subject being studied. Additionally, the accuracy of the results depends on the quality of the available data and the statistical methods used. Inaccurate data or inappropriate hypothesis formulation may lead to incorrect conclusions or failed tests. Hypothesis testing can also lead to errors, such as analysts either accepting or rejecting a null hypothesis when they shouldn’t have. These errors may result in false conclusions or missed opportunities to identify significant patterns or relationships in the data.
Hypothesis testing refers to a statistical process that helps researchers determine the reliability of a study. By using a well-formulated hypothesis and set of statistical tests, individuals or businesses can make inferences about the population that they are studying and draw conclusions based on the data presented. All hypothesis testing methods have the same four-step process, which includes stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.
Sage. " Introduction to Hypothesis Testing ," Page 4.
Elder Research. " Who Invented the Null Hypothesis? "
Formplus. " Hypothesis Testing: Definition, Uses, Limitations and Examples ."
Statistics By Jim
Making statistics intuitive
By Jim Frost 10 Comments
A test statistic assesses how consistent your sample data are with the null hypothesis in a hypothesis test. Test statistic calculations take your sample data and boil them down to a single number that quantifies how much your sample diverges from the null hypothesis. As a test statistic value becomes more extreme, it indicates larger differences between your sample data and the null hypothesis.
When your test statistic indicates a sufficiently large incompatibility with the null hypothesis, you can reject the null and state that your results are statistically significant—your data support the notion that the sample effect exists in the population . To use a test statistic to evaluate statistical significance, you either compare it to a critical value or use it to calculate the p-value .
Statisticians named the hypothesis tests after the test statistics because they’re the quantity that the tests actually evaluate. For example, t-tests assess t-values, F-tests evaluate F-values, and chi-square tests use, you guessed it, chi-square values.
In this post, learn about test statistics, how to calculate them, interpret them, and evaluate statistical significance using the critical value and p-value methods.
Each test statistic has its own formula. I present several common test statistics examples below. To see worked examples for each one, click the links to my more detailed articles.
T-value for 1-sample t-test | Take the sample mean, subtract the hypothesized mean, and divide by the . | |
T-value for 2-sample t-test | Take one sample mean, subtract the other, and divide by the pooled standard deviation. | |
F-value for F-tests and ANOVA | Calculate the ratio of two . | |
Chi-squared value (χ ) for a Chi-squared test | Sum the squared differences between observed and expected values divided by the expected values. |
In the formulas above, it’s helpful to understand the null condition and the test statistic value that occurs when your sample data match that condition exactly. Also, it’s worthwhile knowing what causes the test statistics to move further away from the null value, potentially becoming significant. Test statistics are statistically significant when they exceed a critical value.
All these test statistics are ratios, which helps you understand their null values.
When a t-value equals 0, it indicates that your sample data match the null hypothesis exactly.
For a 1-sample t-test, when the sample mean equals the hypothesized mean, the numerator is zero, which causes the entire t-value ratio to equal zero. As the sample mean moves away from the hypothesized mean in either the positive or negative direction, the test statistic moves away from zero in the same direction.
A similar case exists for 2-sample t-tests. When the two sample means are equal, the numerator is zero, and the entire test statistic ratio is zero. As the two sample means become increasingly different, the absolute value of the numerator increases, and the t-value becomes more positive or negative.
Related post : How T-tests Work
When an F-value equals 1, it indicates that the two variances in the numerator and denominator are equal, matching the null hypothesis.
As the numerator and denominator become less and less similar, the F-value moves away from one in either direction.
Related post : The F-test in ANOVA
When a chi-squared value equals 0, it indicates that the observed values always match the expected values. This condition causes the numerator to equal zero, making the chi-squared value equal zero.
As the observed values progressively fail to match the expected values, the numerator increases, causing the test statistic to rise from zero.
Related post : How a Chi-Squared Test Works
You’ll never see a test statistic that equals the null value precisely in practice. However, trivial differences been sample values and the null value are not uncommon.
Test statistics are unitless. This fact can make them difficult to interpret on their own. You know they evaluate how well your data agree with the null hypothesis. If your test statistic is extreme enough, your data are so incompatible with the null hypothesis that you can reject it and conclude that your results are statistically significant. But how does that translate to specific values of your test statistic? Where do you draw the line?
For instance, t-values of zero match the null value. But how far from zero should your t-value be to be statistically significant? Is 1 enough? 2? 3? If your t-value is 2, what does it mean anyway? In this case, we know that the sample mean doesn’t equal the null value, but how exceptional is it? To complicate matters, the dividing line changes depending on your sample size and other study design issues.
Similar types of questions apply to the other test statistics too.
To interpret individual values of a test statistic, we need to place them in a larger context. Towards this end, let me introduce you to sampling distributions for test statistics!
Performing a hypothesis test on a sample produces a single test statistic. Now, imagine you carry out the following process:
This process produces the distribution of test statistic values that occurs when the effect does not exist in the population (i.e., the null hypothesis is true). Statisticians refer to this type of distribution as a sampling distribution, a kind of probability distribution.
Why would we need this type of distribution?
It provides the larger context required for interpreting a test statistic. More specifically, it allows us to compare our study’s single test statistic to values likely to occur when the null is true. We can quantify our sample statistic’s rareness while assuming the effect does not exist in the population. Now that’s helpful!
Fortunately, we don’t need to collect many random samples to create this distribution! Statisticians have developed formulas allowing us to estimate sampling distributions for test statistics using the sample data.
To evaluate your data’s compatibility with the null hypothesis, place your study’s test statistic in the distribution.
Related post : Understanding Probability Distributions
Suppose our t-test produces a t-value of two. That’s our test statistic. Let’s see where it fits in.
The sampling distribution below shows a t-distribution with 20 degrees of freedom, equating to a 1-sample t-test with a sample size of 21. The distribution centers on zero because it assumes the null hypothesis is correct. When the null is true, your analysis is most likely to obtain a t-value near zero and less likely to produce t-values further from zero in either direction.
The sampling distribution indicates that our test statistic is somewhat rare when we assume the null hypothesis is correct. However, the chances of observing t-values from -2 to +2 are not totally inconceivable. We need a way to quantify the likelihood.
From this point, we need to use the sampling distributions’ ability to calculate probabilities for test statistics.
Related post : Sampling Distributions Explained
The significance level uses critical values to define how far the test statistic must be from the null value to reject the null hypothesis. When the test statistic exceeds a critical value, the results are statistically significant.
The percentage of the area beneath the sampling distribution curve that is shaded represents the probability that the test statistic will fall in those regions when the null is true. Consequently, to depict a significance level of 0.05, I’ll shade 5% of the sampling distribution furthest away from the null value.
The two shaded areas are equidistant from the null value in the center. Each region has a likelihood of 0.025, which sums to our significance level of 0.05. These shaded areas are the critical regions for a two-tailed hypothesis test. Let’s return to our example t-value of 2.
Related post : What are Critical Values?
In this example, the critical values are -2.086 and +2.086. Our test statistic of 2 is not statistically significant because it does not exceed the critical value.
Other hypothesis tests have their own test statistics and sampling distributions, but their processes for critical values are generally similar.
Learn how to find critical values for test statistics using tables:
Related post : Understanding Significance Levels
P-values are the probability of observing an effect at least as extreme as your sample’s effect if you assume no effect exists in the population.
Test statistics represent effect sizes in hypothesis tests because they denote the difference between your sample effect and no effect —the null hypothesis. Consequently, you use the test statistic to calculate the p-value for your hypothesis test.
The above p-value definition is a bit tortuous. Fortunately, it’s much easier to understand how test statistics and p-values work together using a sampling distribution graph.
Let’s use our hypothetical test statistic t-value of 2 for this example. However, because I’m displaying the results of a two-tailed test, I need to use t-values of +2 and -2 to cover both tails.
Related post : One-tailed vs. Two-Tailed Hypothesis Tests
The graph below displays the probability of t-values less than -2 and greater than +2 using the area under the curve. This graph is specific to our t-test design (1-sample t-test with N = 21).
The sampling distribution indicates that each of the two shaded regions has a probability of 0.02963—for a total of 0.05926. That’s the p-value! The graph shows that the test statistic falls within these areas almost 6% of the time when the null hypothesis is true in the population.
While this likelihood seems small, it’s not low enough to justify rejecting the null under the standard significance level of 0.05. P-value results are always consistent with the critical value method. Learn more about using test statistics to find p values .
While test statistics are a crucial part of hypothesis testing, you’ll probably let your statistical software calculate the p-value for the test. However, understanding test statistics will boost your comprehension of what a hypothesis test actually assesses.
Related post : Interpreting P-values
July 5, 2024 at 8:21 am
“As the observed values progressively fail to match the observed values, the numerator increases, causing the test statistic to rise from zero”.
Sir, this sentence is written in the Chi-squared Test heading. There the observed value is written twice. I think the second one to be replaced with ‘expected values’.
July 5, 2024 at 4:10 pm
Thanks so much, Dr. Raj. You’re correct about the typo and I’ve made the correction.
May 9, 2024 at 1:40 am
Thank you very much (great page on one and two-tailed tests)!
May 6, 2024 at 12:17 pm
I would like to ask a question. If only positive numbers are the possible values in a sample (e.g. absolute values without 0), is it meaningful to test if the sample is significantly different from zero (using for example a one sample t-test or a Wilcoxon signed-rank test) or can I assume that if given a large enough sample, the result will by definition be significant (even if a small or very variable sample results in a non-significant hypothesis test).
Thank you very much,
May 6, 2024 at 4:35 pm
If you’re talking about the raw values you’re assessing using a one-sample t-test, it doesn’t make sense to compare them to zero given your description of the data. You know that the mean can’t possibly equal zero. The mean must be some positive value. Yes, in this scenario, if you have a large enough sample size, you should get statistically significant results. So, that t-test isn’t tell you anything that you don’t already know!
However, you should be aware of several things. The 1-sample test can compare your sample mean to values other than zero. Typically, you’ll need to specify the value of the null hypothesis for your software. This value is the comparison value. The test determines whether your sample data provide enough evidence to conclude that the population mean does not equal the null hypothesis value you specify. You’ll need to specify the value because there is no obvious default value to use. Every 1-sample t-test has its subject-area context with a value that makes sense for its null hypothesis value and it is frequently not zero.
I suspect that you’re getting tripped up with the fact that t-tests use a t-value of zero for its null hypothesis value. That doesn’t mean your 1-sample t-test is comparing your sample mean to zero. The test converts your data to a single t-value and compares the t-value to zero. But your actual null hypothesis value can be something else. It’s just converting your sample to a standardized value to use for testing. So, while the t-test compares your sample’s t-value to zero, you can actually compare your sample mean to any value you specify. You need to use a value that makes sense for your subject area.
I hope that makes sense!
May 8, 2024 at 8:37 am
Thank you very much Jim, this helps a lot! Actually, the value I would like to compare my sample to is zero, but I just couldn’t find the right way to test it apparently (it’s about EEG data). The original data was a sample of numbers between -1 and +1, with the question if they are significantly different from zero in either direction (in which case a one sample t-test makes sense I guess, since the sample mean can in fact be zero). However, since a sample mean of 0 can also occur if half of the sample differs in the negative, and the other half in the positive direction, I also wanted to test if there is a divergence from 0 in ‘absolute’ terms – that’s how the absolute valued numbers came about (I know that absolute values can also be zero, but in this specific case, they were all positive numbers) And a special thanks for the last paragraph – I will definitely keep in mind, it is a potential point of confusion.
May 8, 2024 at 8:33 pm
You can use a 1-sample t test for both cases but you’ll need to set them up slightly different. To detect a positive or negative difference from zero, use a 2-tailed test. For the case with absolute values, use a one-tailed test with a critical region in the positive end. To learn more, read about One- and Two-Tailed Tests Explained . Use zero for the comparison value in both cases.
February 12, 2024 at 1:00 am
Very helpful and well articulated! Thanks Jim 🙂
September 18, 2023 at 10:01 am
Thank you for brief explanation.
July 25, 2022 at 8:32 am
the content was helpful to me. thank you
Hypothesis is a hypothesis is fundamental concept in the world of research and statistics. It is a testable statement that explains what is happening or observed. It proposes the relation between the various participating variables.
Hypothesis is also called Theory, Thesis, Guess, Assumption, or Suggestion . Hypothesis creates a structure that guides the search for knowledge.
In this article, we will learn what hypothesis is, its characteristics, types, and examples. We will also learn how hypothesis helps in scientific research.
Table of Content
Characteristics of hypothesis, sources of hypothesis, types of hypothesis, functions of hypothesis, how hypothesis help in scientific research.
Hypothesis is a suggested idea or an educated guess or a proposed explanation made based on limited evidence, serving as a starting point for further study. They are meant to lead to more investigation.
It’s mainly a smart guess or suggested answer to a problem that can be checked through study and trial. In science work, we make guesses called hypotheses to try and figure out what will happen in tests or watching. These are not sure things but rather ideas that can be proved or disproved based on real-life proofs. A good theory is clear and can be tested and found wrong if the proof doesn’t support it.
A hypothesis is a proposed statement that is testable and is given for something that happens or observed.
Here are some key characteristics of a hypothesis:
Hypotheses can come from different places based on what you’re studying and the kind of research. Here are some common sources from which hypotheses may originate:
Here are some common types of hypotheses:
Complex hypothesis, directional hypothesis.
Alternative hypothesis (h1 or ha), statistical hypothesis, research hypothesis, associative hypothesis, causal hypothesis.
Simple Hypothesis guesses a connection between two things. It says that there is a connection or difference between variables, but it doesn’t tell us which way the relationship goes. Example: Studying more can help you do better on tests. Getting more sun makes people have higher amounts of vitamin D.
Complex Hypothesis tells us what will happen when more than two things are connected. It looks at how different things interact and may be linked together. Example: How rich you are, how easy it is to get education and healthcare greatly affects the number of years people live. A new medicine’s success relies on the amount used, how old a person is who takes it and their genes.
Directional Hypothesis says how one thing is related to another. For example, it guesses that one thing will help or hurt another thing. Example: Drinking more sweet drinks is linked to a higher body weight score. Too much stress makes people less productive at work.
Non-Directional Hypothesis are the one that don’t say how the relationship between things will be. They just say that there is a connection, without telling which way it goes. Example: Drinking caffeine can affect how well you sleep. People often like different kinds of music based on their gender.
Null hypothesis is a statement that says there’s no connection or difference between different things. It implies that any seen impacts are because of luck or random changes in the information. Example: The average test scores of Group A and Group B are not much different. There is no connection between using a certain fertilizer and how much it helps crops grow.
Alternative Hypothesis is different from the null hypothesis and shows that there’s a big connection or gap between variables. Scientists want to say no to the null hypothesis and choose the alternative one. Example: Patients on Diet A have much different cholesterol levels than those following Diet B. Exposure to a certain type of light can change how plants grow compared to normal sunlight.
Statistical Hypothesis are used in math testing and include making ideas about what groups or bits of them look like. You aim to get information or test certain things using these top-level, common words only. Example: The average smarts score of kids in a certain school area is 100. The usual time it takes to finish a job using Method A is the same as with Method B.
Research Hypothesis comes from the research question and tells what link is expected between things or factors. It leads the study and chooses where to look more closely. Example: Having more kids go to early learning classes helps them do better in school when they get older. Using specific ways of talking affects how much customers get involved in marketing activities.
Associative Hypothesis guesses that there is a link or connection between things without really saying it caused them. It means that when one thing changes, it is connected to another thing changing. Example: Regular exercise helps to lower the chances of heart disease. Going to school more can help people make more money.
Causal Hypothesis are different from other ideas because they say that one thing causes another. This means there’s a cause and effect relationship between variables involved in the situation. They say that when one thing changes, it directly makes another thing change. Example: Playing violent video games makes teens more likely to act aggressively. Less clean air directly impacts breathing health in city populations.
Hypotheses have many important jobs in the process of scientific research. Here are the key functions of hypotheses:
Researchers use hypotheses to put down their thoughts directing how the experiment would take place. Following are the steps that are involved in the scientific method:
Mathematics Maths Formulas Branches of Mathematics
Hypothesis is a testable statement serving as an initial explanation for phenomena, based on observations, theories, or existing knowledge . It acts as a guiding light for scientific research, proposing potential relationships between variables that can be empirically tested through experiments and observations.
The hypothesis must be specific, testable, falsifiable, and grounded in prior research or observation, laying out a predictive, if-then scenario that details a cause-and-effect relationship. It originates from various sources including existing theories, observations, previous research, and even personal curiosity, leading to different types, such as simple, complex, directional, non-directional, null, and alternative hypotheses, each serving distinct roles in research methodology .
The hypothesis not only guides the research process by shaping objectives and designing experiments but also facilitates objective analysis and interpretation of data , ultimately driving scientific progress through a cycle of testing, validation, and refinement.
What is a hypothesis.
A guess is a possible explanation or forecast that can be checked by doing research and experiments.
The components of a Hypothesis are Independent Variable, Dependent Variable, Relationship between Variables, Directionality etc.
Testability, Falsifiability, Clarity and Precision, Relevance are some parameters that makes a Good Hypothesis
You cannot prove conclusively that most hypotheses are true because it’s generally impossible to examine all possible cases for exceptions that would disprove them.
Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data
Yes, you can change or improve your ideas based on new information discovered during the research process.
Hypotheses are used to support scientific research and bring about advancements in knowledge.
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Formulate the Hypotheses: Write your research hypotheses as a null hypothesis (H 0) and an alternative hypothesis (H A).; Data Collection: Gather data specifically aimed at testing the hypothesis.; Conduct A Test: Use a suitable statistical test to analyze your data.; Make a Decision: Based on the statistical test results, decide whether to reject the null hypothesis or fail to reject it.
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In hypothesis testing, the goal is to see if there is sufficient statistical evidence to reject a presumed null hypothesis in favor of a conjectured alternative hypothesis.The null hypothesis is usually denoted \(H_0\) while the alternative hypothesis is usually denoted \(H_1\). An hypothesis test is a statistical decision; the conclusion will either be to reject the null hypothesis in favor ...
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The null hypothesis has the same parameter and number with an equal sign. H0: μ = $30, 000 HA: μ> $30, 000. b. x = number od students who like math. p = proportion of students who like math. The guess is that p < 0.10 and that is the alternative hypothesis. H0: p = 0.10 HA: p <0.10. c. x = age of students in this class.
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A statistical hypothesis is an assumption about a population parameter. This assumption may or may not be true. Hypothesis testing refers to the formal procedures used by statisticians to accept or reject statistical hypotheses. Statistical Hypotheses. The best way to determine whether a statistical hypothesis is true would be to examine the ...
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