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Hypothesis Testing | A Step-by-Step Guide with Easy Examples

Published on November 8, 2019 by Rebecca Bevans . Revised on June 22, 2023.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics . It is most often used by scientists to test specific predictions, called hypotheses, that arise from theories.

There are 5 main steps in hypothesis testing:

State your research hypothesis as a null hypothesis and alternate hypothesis (H o ) and (H a or H 1 ).
Collect data in a way designed to test the hypothesis.
Perform an appropriate statistical test .
Decide whether to reject or fail to reject your null hypothesis.
Present the findings in your results and discussion section.

Though the specific details might vary, the procedure you will use when testing a hypothesis will always follow some version of these steps.

Step 1: state your null and alternate hypothesis, step 2: collect data, step 3: perform a statistical test, step 4: decide whether to reject or fail to reject your null hypothesis, step 5: present your findings, other interesting articles, frequently asked questions about hypothesis testing.

After developing your initial research hypothesis (the prediction that you want to investigate), it is important to restate it as a null (H o ) and alternate (H a ) hypothesis so that you can test it mathematically.

The alternate hypothesis is usually your initial hypothesis that predicts a relationship between variables. The null hypothesis is a prediction of no relationship between the variables you are interested in.

H 0 : Men are, on average, not taller than women. H a : Men are, on average, taller than women.

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For a statistical test to be valid , it is important to perform sampling and collect data in a way that is designed to test your hypothesis. If your data are not representative, then you cannot make statistical inferences about the population you are interested in.

There are a variety of statistical tests available, but they are all based on the comparison of within-group variance (how spread out the data is within a category) versus between-group variance (how different the categories are from one another).

If the between-group variance is large enough that there is little or no overlap between groups, then your statistical test will reflect that by showing a low p -value . This means it is unlikely that the differences between these groups came about by chance.

Alternatively, if there is high within-group variance and low between-group variance, then your statistical test will reflect that with a high p -value. This means it is likely that any difference you measure between groups is due to chance.

Your choice of statistical test will be based on the type of variables and the level of measurement of your collected data .

an estimate of the difference in average height between the two groups.
a p -value showing how likely you are to see this difference if the null hypothesis of no difference is true.

Based on the outcome of your statistical test, you will have to decide whether to reject or fail to reject your null hypothesis.

In most cases you will use the p -value generated by your statistical test to guide your decision. And in most cases, your predetermined level of significance for rejecting the null hypothesis will be 0.05 – that is, when there is a less than 5% chance that you would see these results if the null hypothesis were true.

In some cases, researchers choose a more conservative level of significance, such as 0.01 (1%). This minimizes the risk of incorrectly rejecting the null hypothesis ( Type I error ).

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The results of hypothesis testing will be presented in the results and discussion sections of your research paper , dissertation or thesis .

In the results section you should give a brief summary of the data and a summary of the results of your statistical test (for example, the estimated difference between group means and associated p -value). In the discussion , you can discuss whether your initial hypothesis was supported by your results or not.

In the formal language of hypothesis testing, we talk about rejecting or failing to reject the null hypothesis. You will probably be asked to do this in your statistics assignments.

However, when presenting research results in academic papers we rarely talk this way. Instead, we go back to our alternate hypothesis (in this case, the hypothesis that men are on average taller than women) and state whether the result of our test did or did not support the alternate hypothesis.

If your null hypothesis was rejected, this result is interpreted as “supported the alternate hypothesis.”

These are superficial differences; you can see that they mean the same thing.

You might notice that we don’t say that we reject or fail to reject the alternate hypothesis . This is because hypothesis testing is not designed to prove or disprove anything. It is only designed to test whether a pattern we measure could have arisen spuriously, or by chance.

If we reject the null hypothesis based on our research (i.e., we find that it is unlikely that the pattern arose by chance), then we can say our test lends support to our hypothesis . But if the pattern does not pass our decision rule, meaning that it could have arisen by chance, then we say the test is inconsistent with our hypothesis .

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

Normal distribution
Descriptive statistics
Measures of central tendency
Correlation coefficient

Methodology

Cluster sampling
Stratified sampling
Types of interviews
Cohort study
Thematic analysis

Research bias

Implicit bias
Cognitive bias
Survivorship bias
Availability heuristic
Nonresponse bias
Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

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Statistics Made Easy

Introduction to Hypothesis Testing

A statistical hypothesis is an assumption about a population parameter .

For example, we may assume that the mean height of a male in the U.S. is 70 inches.

The assumption about the height is the statistical hypothesis and the true mean height of a male in the U.S. is the population parameter .

A hypothesis test is a formal statistical test we use to reject or fail to reject a statistical hypothesis.

The Two Types of Statistical Hypotheses

To test whether a statistical hypothesis about a population parameter is true, we obtain a random sample from the population and perform a hypothesis test on the sample data.

There are two types of statistical hypotheses:

The null hypothesis , denoted as H 0 , is the hypothesis that the sample data occurs purely from chance.

The alternative hypothesis , denoted as H 1 or H a , is the hypothesis that the sample data is influenced by some non-random cause.

Hypothesis Tests

A hypothesis test consists of five steps:

1. State the hypotheses.

State the null and alternative hypotheses. These two hypotheses need to be mutually exclusive, so if one is true then the other must be false.

2. Determine a significance level to use for the hypothesis.

Decide on a significance level. Common choices are .01, .05, and .1.

3. Find the test statistic.

Find the test statistic and the corresponding p-value. Often we are analyzing a population mean or proportion and the general formula to find the test statistic is: (sample statistic – population parameter) / (standard deviation of statistic)

4. Reject or fail to reject the null hypothesis.

Using the test statistic or the p-value, determine if you can reject or fail to reject the null hypothesis based on the significance level.

The p-value tells us the strength of evidence in support of a null hypothesis. If the p-value is less than the significance level, we reject the null hypothesis.

5. Interpret the results.

Interpret the results of the hypothesis test in the context of the question being asked.

The Two Types of Decision Errors

There are two types of decision errors that one can make when doing a hypothesis test:

Type I error: You reject the null hypothesis when it is actually true. The probability of committing a Type I error is equal to the significance level, often called alpha , and denoted as α.

Type II error: You fail to reject the null hypothesis when it is actually false. The probability of committing a Type II error is called the Power of the test or Beta , denoted as β.

One-Tailed and Two-Tailed Tests

A statistical hypothesis can be one-tailed or two-tailed.

A one-tailed hypothesis involves making a “greater than” or “less than ” statement.

For example, suppose we assume the mean height of a male in the U.S. is greater than or equal to 70 inches. The null hypothesis would be H0: µ ≥ 70 inches and the alternative hypothesis would be Ha: µ < 70 inches.

A two-tailed hypothesis involves making an “equal to” or “not equal to” statement.

For example, suppose we assume the mean height of a male in the U.S. is equal to 70 inches. The null hypothesis would be H0: µ = 70 inches and the alternative hypothesis would be Ha: µ ≠ 70 inches.

Note: The “equal” sign is always included in the null hypothesis, whether it is =, ≥, or ≤.

Related: What is a Directional Hypothesis?

Types of Hypothesis Tests

There are many different types of hypothesis tests you can perform depending on the type of data you’re working with and the goal of your analysis.

The following tutorials provide an explanation of the most common types of hypothesis tests:

Introduction to the One Sample t-test Introduction to the Two Sample t-test Introduction to the Paired Samples t-test Introduction to the One Proportion Z-Test Introduction to the Two Proportion Z-Test

Published by Zach

An Introduction to Statistics: Understanding Hypothesis Testing and Statistical Errors

Priya ranganathan.

1 Department of Anesthesiology, Critical Care and Pain, Tata Memorial Hospital, Mumbai, Maharashtra, India

2 Department of Surgical Oncology, Tata Memorial Centre, Mumbai, Maharashtra, India

The second article in this series on biostatistics covers the concepts of sample, population, research hypotheses and statistical errors.

How to cite this article

Ranganathan P, Pramesh CS. An Introduction to Statistics: Understanding Hypothesis Testing and Statistical Errors. Indian J Crit Care Med 2019;23(Suppl 3):S230–S231.

Two papers quoted in this issue of the Indian Journal of Critical Care Medicine report. The results of studies aim to prove that a new intervention is better than (superior to) an existing treatment. In the ABLE study, the investigators wanted to show that transfusion of fresh red blood cells would be superior to standard-issue red cells in reducing 90-day mortality in ICU patients. 1 The PROPPR study was designed to prove that transfusion of a lower ratio of plasma and platelets to red cells would be superior to a higher ratio in decreasing 24-hour and 30-day mortality in critically ill patients. 2 These studies are known as superiority studies (as opposed to noninferiority or equivalence studies which will be discussed in a subsequent article).

SAMPLE VERSUS POPULATION

A sample represents a group of participants selected from the entire population. Since studies cannot be carried out on entire populations, researchers choose samples, which are representative of the population. This is similar to walking into a grocery store and examining a few grains of rice or wheat before purchasing an entire bag; we assume that the few grains that we select (the sample) are representative of the entire sack of grains (the population).

The results of the study are then extrapolated to generate inferences about the population. We do this using a process known as hypothesis testing. This means that the results of the study may not always be identical to the results we would expect to find in the population; i.e., there is the possibility that the study results may be erroneous.

HYPOTHESIS TESTING

A clinical trial begins with an assumption or belief, and then proceeds to either prove or disprove this assumption. In statistical terms, this belief or assumption is known as a hypothesis. Counterintuitively, what the researcher believes in (or is trying to prove) is called the “alternate” hypothesis, and the opposite is called the “null” hypothesis; every study has a null hypothesis and an alternate hypothesis. For superiority studies, the alternate hypothesis states that one treatment (usually the new or experimental treatment) is superior to the other; the null hypothesis states that there is no difference between the treatments (the treatments are equal). For example, in the ABLE study, we start by stating the null hypothesis—there is no difference in mortality between groups receiving fresh RBCs and standard-issue RBCs. We then state the alternate hypothesis—There is a difference between groups receiving fresh RBCs and standard-issue RBCs. It is important to note that we have stated that the groups are different, without specifying which group will be better than the other. This is known as a two-tailed hypothesis and it allows us to test for superiority on either side (using a two-sided test). This is because, when we start a study, we are not 100% certain that the new treatment can only be better than the standard treatment—it could be worse, and if it is so, the study should pick it up as well. One tailed hypothesis and one-sided statistical testing is done for non-inferiority studies, which will be discussed in a subsequent paper in this series.

STATISTICAL ERRORS

There are two possibilities to consider when interpreting the results of a superiority study. The first possibility is that there is truly no difference between the treatments but the study finds that they are different. This is called a Type-1 error or false-positive error or alpha error. This means falsely rejecting the null hypothesis.

The second possibility is that there is a difference between the treatments and the study does not pick up this difference. This is called a Type 2 error or false-negative error or beta error. This means falsely accepting the null hypothesis.

The power of the study is the ability to detect a difference between groups and is the converse of the beta error; i.e., power = 1-beta error. Alpha and beta errors are finalized when the protocol is written and form the basis for sample size calculation for the study. In an ideal world, we would not like any error in the results of our study; however, we would need to do the study in the entire population (infinite sample size) to be able to get a 0% alpha and beta error. These two errors enable us to do studies with realistic sample sizes, with the compromise that there is a small possibility that the results may not always reflect the truth. The basis for this will be discussed in a subsequent paper in this series dealing with sample size calculation.

Conventionally, type 1 or alpha error is set at 5%. This means, that at the end of the study, if there is a difference between groups, we want to be 95% certain that this is a true difference and allow only a 5% probability that this difference has occurred by chance (false positive). Type 2 or beta error is usually set between 10% and 20%; therefore, the power of the study is 90% or 80%. This means that if there is a difference between groups, we want to be 80% (or 90%) certain that the study will detect that difference. For example, in the ABLE study, sample size was calculated with a type 1 error of 5% (two-sided) and power of 90% (type 2 error of 10%) (1).

Table 1 gives a summary of the two types of statistical errors with an example

Statistical errors

In the next article in this series, we will look at the meaning and interpretation of ‘ p ’ value and confidence intervals for hypothesis testing.

Source of support: Nil

Conflict of interest: None

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Fundamental Analysis

Hypothesis to Be Tested: Definition and 4 Steps for Testing with Example

What Is Hypothesis Testing?

Hypothesis testing, sometimes called significance testing, is an act in statistics whereby an analyst tests an assumption regarding a population parameter. The methodology employed by the analyst depends on the nature of the data used and the reason for the analysis.

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data. Such data may come from a larger population, or from a data-generating process. The word "population" will be used for both of these cases in the following descriptions.

Key Takeaways

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data.
The test provides evidence concerning the plausibility of the hypothesis, given the data.
Statistical analysts test a hypothesis by measuring and examining a random sample of the population being analyzed.
The four steps of hypothesis testing include stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

How Hypothesis Testing Works

In hypothesis testing, an analyst tests a statistical sample, with the goal of providing evidence on the plausibility of the null hypothesis.

Statistical analysts test a hypothesis by measuring and examining a random sample of the population being analyzed. All analysts use a random population sample to test two different hypotheses: the null hypothesis and the alternative hypothesis.

The null hypothesis is usually a hypothesis of equality between population parameters; e.g., a null hypothesis may state that the population mean return is equal to zero. The alternative hypothesis is effectively the opposite of a null hypothesis (e.g., the population mean return is not equal to zero). Thus, they are mutually exclusive , and only one can be true. However, one of the two hypotheses will always be true.

The null hypothesis is a statement about a population parameter, such as the population mean, that is assumed to be true.

4 Steps of Hypothesis Testing

All hypotheses are tested using a four-step process:

The first step is for the analyst to state the hypotheses.
The second step is to formulate an analysis plan, which outlines how the data will be evaluated.
The third step is to carry out the plan and analyze the sample data.
The final step is to analyze the results and either reject the null hypothesis, or state that the null hypothesis is plausible, given the data.

Real-World Example of Hypothesis Testing

If, for example, a person wants to test that a penny has exactly a 50% chance of landing on heads, the null hypothesis would be that 50% is correct, and the alternative hypothesis would be that 50% is not correct.

Mathematically, the null hypothesis would be represented as Ho: P = 0.5. The alternative hypothesis would be denoted as "Ha" and be identical to the null hypothesis, except with the equal sign struck-through, meaning that it does not equal 50%.

A random sample of 100 coin flips is taken, and the null hypothesis is then tested. If it is found that the 100 coin flips were distributed as 40 heads and 60 tails, the analyst would assume that a penny does not have a 50% chance of landing on heads and would reject the null hypothesis and accept the alternative hypothesis.

If, on the other hand, there were 48 heads and 52 tails, then it is plausible that the coin could be fair and still produce such a result. In cases such as this where the null hypothesis is "accepted," the analyst states that the difference between the expected results (50 heads and 50 tails) and the observed results (48 heads and 52 tails) is "explainable by chance alone."

Some staticians attribute the first hypothesis tests to satirical writer John Arbuthnot in 1710, who studied male and female births in England after observing that in nearly every year, male births exceeded female births by a slight proportion. Arbuthnot calculated that the probability of this happening by chance was small, and therefore it was due to “divine providence.”

What is Hypothesis Testing?

Hypothesis testing refers to a process used by analysts to assess the plausibility of a hypothesis by using sample data. In hypothesis testing, statisticians formulate two hypotheses: the null hypothesis and the alternative hypothesis. A null hypothesis determines there is no difference between two groups or conditions, while the alternative hypothesis determines that there is a difference. Researchers evaluate the statistical significance of the test based on the probability that the null hypothesis is true.

What are the Four Key Steps Involved in Hypothesis Testing?

Hypothesis testing begins with an analyst stating two hypotheses, with only one that can be right. The analyst then formulates an analysis plan, which outlines how the data will be evaluated. Next, they move to the testing phase and analyze the sample data. Finally, the analyst analyzes the results and either rejects the null hypothesis or states that the null hypothesis is plausible, given the data.

What are the Benefits of Hypothesis Testing?

Hypothesis testing helps assess the accuracy of new ideas or theories by testing them against data. This allows researchers to determine whether the evidence supports their hypothesis, helping to avoid false claims and conclusions. Hypothesis testing also provides a framework for decision-making based on data rather than personal opinions or biases. By relying on statistical analysis, hypothesis testing helps to reduce the effects of chance and confounding variables, providing a robust framework for making informed conclusions.

What are the Limitations of Hypothesis Testing?

Hypothesis testing relies exclusively on data and doesn’t provide a comprehensive understanding of the subject being studied. Additionally, the accuracy of the results depends on the quality of the available data and the statistical methods used. Inaccurate data or inappropriate hypothesis formulation may lead to incorrect conclusions or failed tests. Hypothesis testing can also lead to errors, such as analysts either accepting or rejecting a null hypothesis when they shouldn’t have. These errors may result in false conclusions or missed opportunities to identify significant patterns or relationships in the data.

The Bottom Line

Hypothesis testing refers to a statistical process that helps researchers and/or analysts determine the reliability of a study. By using a well-formulated hypothesis and set of statistical tests, individuals or businesses can make inferences about the population that they are studying and draw conclusions based on the data presented. There are different types of hypothesis testing, each with their own set of rules and procedures. However, all hypothesis testing methods have the same four step process, which includes stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result. Hypothesis testing plays a vital part of the scientific process, helping to test assumptions and make better data-based decisions.

Sage. " Introduction to Hypothesis Testing. " Page 4.

Elder Research. " Who Invented the Null Hypothesis? "

Formplus. " Hypothesis Testing: Definition, Uses, Limitations and Examples. "

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S.3 hypothesis testing.

In reviewing hypothesis tests, we start first with the general idea. Then, we keep returning to the basic procedures of hypothesis testing, each time adding a little more detail.

The general idea of hypothesis testing involves:

Making an initial assumption.
Collecting evidence (data).
Based on the available evidence (data), deciding whether to reject or not reject the initial assumption.

Every hypothesis test — regardless of the population parameter involved — requires the above three steps.

Example S.3.1

Is normal body temperature really 98.6 degrees f section .

Consider the population of many, many adults. A researcher hypothesized that the average adult body temperature is lower than the often-advertised 98.6 degrees F. That is, the researcher wants an answer to the question: "Is the average adult body temperature 98.6 degrees? Or is it lower?" To answer his research question, the researcher starts by assuming that the average adult body temperature was 98.6 degrees F.

Then, the researcher went out and tried to find evidence that refutes his initial assumption. In doing so, he selects a random sample of 130 adults. The average body temperature of the 130 sampled adults is 98.25 degrees.

Then, the researcher uses the data he collected to make a decision about his initial assumption. It is either likely or unlikely that the researcher would collect the evidence he did given his initial assumption that the average adult body temperature is 98.6 degrees:

If it is likely , then the researcher does not reject his initial assumption that the average adult body temperature is 98.6 degrees. There is not enough evidence to do otherwise.
either the researcher's initial assumption is correct and he experienced a very unusual event;
or the researcher's initial assumption is incorrect.

In statistics, we generally don't make claims that require us to believe that a very unusual event happened. That is, in the practice of statistics, if the evidence (data) we collected is unlikely in light of the initial assumption, then we reject our initial assumption.

Example S.3.2

Criminal trial analogy section .

One place where you can consistently see the general idea of hypothesis testing in action is in criminal trials held in the United States. Our criminal justice system assumes "the defendant is innocent until proven guilty." That is, our initial assumption is that the defendant is innocent.

In the practice of statistics, we make our initial assumption when we state our two competing hypotheses -- the null hypothesis ( H 0 ) and the alternative hypothesis ( H A ). Here, our hypotheses are:

H 0 : Defendant is not guilty (innocent)
H A : Defendant is guilty

In statistics, we always assume the null hypothesis is true . That is, the null hypothesis is always our initial assumption.

The prosecution team then collects evidence — such as finger prints, blood spots, hair samples, carpet fibers, shoe prints, ransom notes, and handwriting samples — with the hopes of finding "sufficient evidence" to make the assumption of innocence refutable.

In statistics, the data are the evidence.

The jury then makes a decision based on the available evidence:

If the jury finds sufficient evidence — beyond a reasonable doubt — to make the assumption of innocence refutable, the jury rejects the null hypothesis and deems the defendant guilty. We behave as if the defendant is guilty.
If there is insufficient evidence, then the jury does not reject the null hypothesis . We behave as if the defendant is innocent.

In statistics, we always make one of two decisions. We either "reject the null hypothesis" or we "fail to reject the null hypothesis."

Errors in Hypothesis Testing Section

Did you notice the use of the phrase "behave as if" in the previous discussion? We "behave as if" the defendant is guilty; we do not "prove" that the defendant is guilty. And, we "behave as if" the defendant is innocent; we do not "prove" that the defendant is innocent.

This is a very important distinction! We make our decision based on evidence not on 100% guaranteed proof. Again:

If we reject the null hypothesis, we do not prove that the alternative hypothesis is true.
If we do not reject the null hypothesis, we do not prove that the null hypothesis is true.

We merely state that there is enough evidence to behave one way or the other. This is always true in statistics! Because of this, whatever the decision, there is always a chance that we made an error .

Let's review the two types of errors that can be made in criminal trials:

Table S.3.2 shows how this corresponds to the two types of errors in hypothesis testing.

Note that, in statistics, we call the two types of errors by two different names -- one is called a "Type I error," and the other is called a "Type II error." Here are the formal definitions of the two types of errors:

There is always a chance of making one of these errors. But, a good scientific study will minimize the chance of doing so!

Making the Decision Section

Recall that it is either likely or unlikely that we would observe the evidence we did given our initial assumption. If it is likely , we do not reject the null hypothesis. If it is unlikely , then we reject the null hypothesis in favor of the alternative hypothesis. Effectively, then, making the decision reduces to determining "likely" or "unlikely."

In statistics, there are two ways to determine whether the evidence is likely or unlikely given the initial assumption:

We could take the " critical value approach " (favored in many of the older textbooks).
Or, we could take the " P -value approach " (what is used most often in research, journal articles, and statistical software).

In the next two sections, we review the procedures behind each of these two approaches. To make our review concrete, let's imagine that μ is the average grade point average of all American students who major in mathematics. We first review the critical value approach for conducting each of the following three hypothesis tests about the population mean $\mu$:

In Practice

We would want to conduct the first hypothesis test if we were interested in concluding that the average grade point average of the group is more than 3.
We would want to conduct the second hypothesis test if we were interested in concluding that the average grade point average of the group is less than 3.
And, we would want to conduct the third hypothesis test if we were only interested in concluding that the average grade point average of the group differs from 3 (without caring whether it is more or less than 3).

Upon completing the review of the critical value approach, we review the P -value approach for conducting each of the above three hypothesis tests about the population mean $\mu$. The procedures that we review here for both approaches easily extend to hypothesis tests about any other population parameter.

Hypothesis Testing: Understanding the Basics, Types, and Importance

Hypothesis testing is a statistical method used to determine whether a hypothesis about a population parameter is true or not. This technique helps researchers and decision-makers make informed decisions based on evidence rather than guesses. Hypothesis testing is an essential tool in scientific research, social sciences, and business analysis. In this article, we will delve deeper into the basics of hypothesis testing, types of hypotheses, significance level, p-values, and the importance of hypothesis testing.

Introduction

What is a hypothesis?

What is hypothesis testing, types of hypotheses, null hypothesis, alternative hypothesis, one-tailed and two-tailed tests, significance level and p-values, avoiding type i and type ii errors, making informed decisions, testing business strategies, a/b testing, formulating the null and alternative hypotheses, selecting the appropriate test, setting the level of significance, calculating the p-value, making a decision, common misconceptions about hypothesis testing, understanding hypothesis testing.

A hypothesis is an assumption or a proposition made about a population parameter. It is a statement that can be tested and either supported or refuted. For example, a hypothesis could be that a new medication reduces the severity of symptoms in patients with a particular disease.

Hypothesis testing is a statistical method that helps to determine whether a hypothesis is true or not. It is a procedure that involves collecting and analyzing data to evaluate the probability of the null hypothesis being true. The null hypothesis is the hypothesis that there is no significant difference between a sample and the population.

In hypothesis testing, there are two types of hypotheses: null and alternative.

The null hypothesis, denoted by H0, is a statement of no effect, no relationship, or no difference between the sample and the population. It is assumed to be true until there is sufficient evidence to reject it. For example, the null hypothesis could be that there is no significant difference in the blood pressure of patients who received the medication and those who received a placebo.

The alternative hypothesis, denoted by H1, is a statement of an effect, relationship, or difference between the sample and the population. It is the opposite of the null hypothesis. For example, the alternative hypothesis could be that the medication reduces the blood pressure of patients compared to those who received a placebo.

There are two types of alternative hypotheses: one-tailed and two-tailed. A one-tailed test is used when there is a directional hypothesis. For example, the hypothesis could be that the medication reduces blood pressure. A two-tailed test is used when there is a non-directional hypothesis. For example, the hypothesis could be that there is a significant difference in blood pressure between patients who received the medication and those who received a placebo.

The significance level, denoted by α, is the probability of rejecting the null hypothesis when it is true. It is set at the beginning of the test, usually at 5% or 1%. The p-value is the probability of obtaining a test statistic as extreme as

or more extreme than the observed one, assuming that the null hypothesis is true. If the p-value is less than the significance level, we reject the null hypothesis.

Importance of Hypothesis Testing

Hypothesis testing helps to avoid Type I and Type II errors. Type I error occurs when we reject the null hypothesis when it is actually true. Type II error occurs when we fail to reject the null hypothesis when it is actually false. By setting a significance level and calculating the p-value, we can control the probability of making these errors.

Hypothesis testing helps researchers and decision-makers make informed decisions based on evidence. For example, a medical researcher can use hypothesis testing to determine the effectiveness of a new drug. A business analyst can use hypothesis testing to evaluate the performance of a marketing campaign. By testing hypotheses, decision-makers can avoid making decisions based on guesses or assumptions.

Hypothesis testing is widely used in business analysis to test strategies and make data-driven decisions. For example, a business owner can use hypothesis testing to determine whether a new product will be profitable. By conducting A/B testing, businesses can compare the performance of two versions of a product and make data-driven decisions.

Examples of Hypothesis Testing

A/B testing is a popular technique used in online marketing and web design. It involves comparing two versions of a webpage or an advertisement to determine which one performs better. By conducting A/B testing, businesses can optimize their websites and advertisements to increase conversions and sales.

A t-test is used to compare the means of two samples. It is commonly used in medical research, social sciences, and business analysis. For example, a researcher can use a t-test to determine whether there is a significant difference in the cholesterol levels of patients who received a new drug and those who received a placebo.

Analysis of Variance (ANOVA) is a statistical technique used to compare the means of more than two samples. It is commonly used in medical research, social sciences, and business analysis. For example, a business owner can use ANOVA to determine whether there is a significant difference in the sales performance of three different stores.

Steps in Hypothesis Testing

The first step in hypothesis testing is to formulate the null and alternative hypotheses. The null hypothesis is the hypothesis that there is no significant difference between the sample and the population, while the alternative hypothesis is the opposite.

The second step is to select the appropriate test based on the type of data and the research question. There are different types of tests for different types of data, such as t-test for continuous data and chi-square test for categorical data.

The third step is to set the level of significance, which is usually 5% or 1%. The significance level represents the probability of rejecting the null hypothesis when it is actually true.

The fourth step is to calculate the p-value, which represents the probability of obtaining a test statistic as extreme as or more extreme than the observed one, assuming that the null hypothesis is true.

The final step is to make a decision based on the p-value and the significance level. If the p-value is less than the significance level, we reject the null hypothesis. Otherwise, we fail to reject the null hypothesis.

There are several common misconceptions about hypothesis testing. One of the most common misconceptions is that rejecting the null hypothesis means that the alternative hypothesis is true. However

this is not necessarily the case. Rejecting the null hypothesis only means that there is evidence against it, but it does not prove that the alternative hypothesis is true. Another common misconception is that hypothesis testing can prove causality. However, hypothesis testing can only provide evidence for or against a hypothesis, and causality can only be inferred from a well-designed experiment.

Hypothesis testing is an important statistical technique used to test hypotheses and make informed decisions based on evidence. It helps to avoid Type I and Type II errors, and it is widely used in medical research, social sciences, and business analysis. By following the steps in hypothesis testing and avoiding common misconceptions, researchers and decision-makers can make data-driven decisions and avoid making decisions based on guesses or assumptions.

What is the difference between Type I and Type II errors in hypothesis testing?
Type I error occurs when we reject the null hypothesis when it is actually true, while Type II error occurs when we fail to reject the null hypothesis when it is actually false.
How do you select the appropriate test in hypothesis testing?
The appropriate test is selected based on the type of data and the research question. There are different types of tests for different types of data, such as t-test for continuous data and chi-square test for categorical data.
Can hypothesis testing prove causality?
No, hypothesis testing can only provide evidence for or against a hypothesis, and causality can only be inferred from a well-designed experiment.
Why is hypothesis testing important in business analysis?
Hypothesis testing is important in business analysis because it helps businesses make data-driven decisions and avoid making decisions based on guesses or assumptions. By testing hypotheses, businesses can evaluate the effectiveness of their strategies and optimize their performance.
What is A/B testing?

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2 Responses

This is a great and comprehensive article on hypothesis testing, covering everything from the basics to practical examples. I particularly appreciate the section on common misconceptions, as it’s important to understand what hypothesis testing can and cannot do. Overall, a valuable resource for anyone looking to understand this statistical technique.

Thanks, Ana Carol for your Kind words, Yes these topics are very important to know in Artificial intelligence.

What is a Hypothesis?

A hypothesis is a calculated prediction or assumption about a population parameter based on limited evidence. The whole idea behind hypothesis formulation is testing—this means the researcher subjects his or her calculated assumption to a series of evaluations to know whether they are true or false.

Typically, every research starts with a hypothesis—the investigator makes a claim and experiments to prove that this claim is true or false . For instance, if you predict that students who drink milk before class perform better than those who don’t, then this becomes a hypothesis that can be confirmed or refuted using an experiment.

Read: What is Empirical Research Study? [Examples & Method]

What are the Types of Hypotheses?

1. simple hypothesis.

Also known as a basic hypothesis, a simple hypothesis suggests that an independent variable is responsible for a corresponding dependent variable. In other words, an occurrence of the independent variable inevitably leads to an occurrence of the dependent variable.

Typically, simple hypotheses are considered as generally true, and they establish a causal relationship between two variables.

Examples of Simple Hypothesis

Drinking soda and other sugary drinks can cause obesity.
Smoking cigarettes daily leads to lung cancer.

2. Complex Hypothesis

A complex hypothesis is also known as a modal. It accounts for the causal relationship between two independent variables and the resulting dependent variables. This means that the combination of the independent variables leads to the occurrence of the dependent variables .

Examples of Complex Hypotheses

Adults who do not smoke and drink are less likely to develop liver-related conditions.
Global warming causes icebergs to melt which in turn causes major changes in weather patterns.

3. Null Hypothesis

As the name suggests, a null hypothesis is formed when a researcher suspects that there’s no relationship between the variables in an observation. In this case, the purpose of the research is to approve or disapprove this assumption.

Examples of Null Hypothesis

This is no significant change in a student’s performance if they drink coffee or tea before classes.
There’s no significant change in the growth of a plant if one uses distilled water only or vitamin-rich water.

Read: Research Report: Definition, Types + [Writing Guide]

4. Alternative Hypothesis

To disapprove a null hypothesis, the researcher has to come up with an opposite assumption—this assumption is known as the alternative hypothesis. This means if the null hypothesis says that A is false, the alternative hypothesis assumes that A is true.

An alternative hypothesis can be directional or non-directional depending on the direction of the difference. A directional alternative hypothesis specifies the direction of the tested relationship, stating that one variable is predicted to be larger or smaller than the null value while a non-directional hypothesis only validates the existence of a difference without stating its direction.

Examples of Alternative Hypotheses

Starting your day with a cup of tea instead of a cup of coffee can make you more alert in the morning.
The growth of a plant improves significantly when it receives distilled water instead of vitamin-rich water.

5. Logical Hypothesis

Logical hypotheses are some of the most common types of calculated assumptions in systematic investigations. It is an attempt to use your reasoning to connect different pieces in research and build a theory using little evidence. In this case, the researcher uses any data available to him, to form a plausible assumption that can be tested.

Examples of Logical Hypothesis

Waking up early helps you to have a more productive day.
Beings from Mars would not be able to breathe the air in the atmosphere of the Earth.

6. Empirical Hypothesis

After forming a logical hypothesis, the next step is to create an empirical or working hypothesis. At this stage, your logical hypothesis undergoes systematic testing to prove or disprove the assumption. An empirical hypothesis is subject to several variables that can trigger changes and lead to specific outcomes.

Examples of Empirical Testing

People who eat more fish run faster than people who eat meat.
Women taking vitamin E grow hair faster than those taking vitamin K.

7. Statistical Hypothesis

When forming a statistical hypothesis, the researcher examines the portion of a population of interest and makes a calculated assumption based on the data from this sample. A statistical hypothesis is most common with systematic investigations involving a large target audience. Here, it’s impossible to collect responses from every member of the population so you have to depend on data from your sample and extrapolate the results to the wider population.

Examples of Statistical Hypothesis

45% of students in Louisiana have middle-income parents.
80% of the UK’s population gets a divorce because of irreconcilable differences.

What is Hypothesis Testing?

Hypothesis testing is an assessment method that allows researchers to determine the plausibility of a hypothesis. It involves testing an assumption about a specific population parameter to know whether it’s true or false. These population parameters include variance, standard deviation, and median.

Typically, hypothesis testing starts with developing a null hypothesis and then performing several tests that support or reject the null hypothesis. The researcher uses test statistics to compare the association or relationship between two or more variables.

Explore: Research Bias: Definition, Types + Examples

Researchers also use hypothesis testing to calculate the coefficient of variation and determine if the regression relationship and the correlation coefficient are statistically significant.

How Hypothesis Testing Works

The basis of hypothesis testing is to examine and analyze the null hypothesis and alternative hypothesis to know which one is the most plausible assumption. Since both assumptions are mutually exclusive, only one can be true. In other words, the occurrence of a null hypothesis destroys the chances of the alternative coming to life, and vice-versa.

Interesting: 21 Chrome Extensions for Academic Researchers in 2021

What Are The Stages of Hypothesis Testing?

To successfully confirm or refute an assumption, the researcher goes through five (5) stages of hypothesis testing;

Determine the null hypothesis
Specify the alternative hypothesis
Set the significance level
Calculate the test statistics and corresponding P-value
Draw your conclusion
Determine the Null Hypothesis

Like we mentioned earlier, hypothesis testing starts with creating a null hypothesis which stands as an assumption that a certain statement is false or implausible. For example, the null hypothesis (H0) could suggest that different subgroups in the research population react to a variable in the same way.

Specify the Alternative Hypothesis

Once you know the variables for the null hypothesis, the next step is to determine the alternative hypothesis. The alternative hypothesis counters the null assumption by suggesting the statement or assertion is true. Depending on the purpose of your research, the alternative hypothesis can be one-sided or two-sided.

Using the example we established earlier, the alternative hypothesis may argue that the different sub-groups react differently to the same variable based on several internal and external factors.

Set the Significance Level

Many researchers create a 5% allowance for accepting the value of an alternative hypothesis, even if the value is untrue. This means that there is a 0.05 chance that one would go with the value of the alternative hypothesis, despite the truth of the null hypothesis.

Something to note here is that the smaller the significance level, the greater the burden of proof needed to reject the null hypothesis and support the alternative hypothesis.

Explore: What is Data Interpretation? + [Types, Method & Tools]

Calculate the Test Statistics and Corresponding P-Value

Test statistics in hypothesis testing allow you to compare different groups between variables while the p-value accounts for the probability of obtaining sample statistics if your null hypothesis is true. In this case, your test statistics can be the mean, median and similar parameters.

If your p-value is 0.65, for example, then it means that the variable in your hypothesis will happen 65 in100 times by pure chance. Use this formula to determine the p-value for your data:

Draw Your Conclusions

After conducting a series of tests, you should be able to agree or refute the hypothesis based on feedback and insights from your sample data.

Applications of Hypothesis Testing in Research

Hypothesis testing isn’t only confined to numbers and calculations; it also has several real-life applications in business, manufacturing, advertising, and medicine.

In a factory or other manufacturing plants, hypothesis testing is an important part of quality and production control before the final products are approved and sent out to the consumer.

During ideation and strategy development, C-level executives use hypothesis testing to evaluate their theories and assumptions before any form of implementation. For example, they could leverage hypothesis testing to determine whether or not some new advertising campaign, marketing technique, etc. causes increased sales.

In addition, hypothesis testing is used during clinical trials to prove the efficacy of a drug or new medical method before its approval for widespread human usage.

What is an Example of Hypothesis Testing?

An employer claims that her workers are of above-average intelligence. She takes a random sample of 20 of them and gets the following results:

Mean IQ Scores: 110

Standard Deviation: 15

Mean Population IQ: 100

Step 1: Using the value of the mean population IQ, we establish the null hypothesis as 100.

Step 2: State that the alternative hypothesis is greater than 100.

Step 3: State the alpha level as 0.05 or 5%

Step 4: Find the rejection region area (given by your alpha level above) from the z-table. An area of .05 is equal to a z-score of 1.645.

Step 5: Calculate the test statistics using this formula

Z = (110–100) ÷ (15÷√20)

10 ÷ 3.35 = 2.99

If the value of the test statistics is higher than the value of the rejection region, then you should reject the null hypothesis. If it is less, then you cannot reject the null.

In this case, 2.99 > 1.645 so we reject the null.

Importance/Benefits of Hypothesis Testing

The most significant benefit of hypothesis testing is it allows you to evaluate the strength of your claim or assumption before implementing it in your data set. Also, hypothesis testing is the only valid method to prove that something “is or is not”. Other benefits include:

Hypothesis testing provides a reliable framework for making any data decisions for your population of interest.
It helps the researcher to successfully extrapolate data from the sample to the larger population.
Hypothesis testing allows the researcher to determine whether the data from the sample is statistically significant.
Hypothesis testing is one of the most important processes for measuring the validity and reliability of outcomes in any systematic investigation.
It helps to provide links to the underlying theory and specific research questions.

Criticism and Limitations of Hypothesis Testing

Several limitations of hypothesis testing can affect the quality of data you get from this process. Some of these limitations include:

The interpretation of a p-value for observation depends on the stopping rule and definition of multiple comparisons. This makes it difficult to calculate since the stopping rule is subject to numerous interpretations, plus “multiple comparisons” are unavoidably ambiguous.
Conceptual issues often arise in hypothesis testing, especially if the researcher merges Fisher and Neyman-Pearson’s methods which are conceptually distinct.
In an attempt to focus on the statistical significance of the data, the researcher might ignore the estimation and confirmation by repeated experiments.
Hypothesis testing can trigger publication bias, especially when it requires statistical significance as a criterion for publication.
When used to detect whether a difference exists between groups, hypothesis testing can trigger absurd assumptions that affect the reliability of your observation.

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Type I vs Type II Errors: Causes, Examples & Prevention

This article will discuss the two different types of errors in hypothesis testing and how you can prevent them from occurring in your research

Internal Validity in Research: Definition, Threats, Examples

In this article, we will discuss the concept of internal validity, some clear examples, its importance, and how to test it.

What is Pure or Basic Research? + [Examples & Method]

Simple guide on pure or basic research, its methods, characteristics, advantages, and examples in science, medicine, education and psychology

Alternative vs Null Hypothesis: Pros, Cons, Uses & Examples

We are going to discuss alternative hypotheses and null hypotheses in this post and how they work in research.

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What is a Hypothesis Test?

A quick search for hypothesis tests online gives us several websites with short definitions. Here’s one from a quick definition from the Stat Trek:

A statistical hypothesis is an assumption about a population parameter. This assumption may or may not be true. Hypothesis testing refers to the formal procedures used by statisticians to accept or reject statistical hypotheses.

What is Hypothesis Testing? From Stat Trek

Most websites will have a similar definition or introduction, followed by a number of components, notation, key terminology, and examples.

The Basic Idea

Hypothesis tests show up in many areas of our everyday lives, but they are kind of sneaky. The basic structure of a hypothesis test is very much like a science project from elementary, middle, or high school. You have a problem, hypothesis, data collection, some computations, results or conclusions. What follows next are a few examples of what the hypothesis test and results would look like in journals or other publications, and how those results are presented to the public.

Some Examples of Hypothesis Tests

Example 1: agility testing in youth football (soccer)players; evaluating reliability, validity, and correlates of newly developed testing protocols.

Reactive agility (RAG)and change of direction speed (CODS) were analyzed in 13U and 15U youth soccer players. “Independent samples t-test indicated significant differences between U13 and U15 in S10 (t-test: 3.57, p < 0.001), S20M (t-test: 3.13, p < 0.001), 20Y (t-test: 4.89, p < 0.001), FS_RAG (t-test: 3.96, p < 0.001), and FS_CODS (t-test: 6.42, p < 0.001), with better performance in U15. Starters outperformed non-starters in most capacities among U13, but only in FS_RAG among U15 (t-test: 1.56, p < 0.05).”

Most of this might seem like gibberish for now, but essentially the two groups were analyzed and compared, with significant differences observed between the groups.

Source: https://pubmed.ncbi.nlm.nih.gov/31906269/

Example 2: Manual therapy in the treatment of carpal tunnel syndrome in diabetic patients: A randomized clinical trial

Thirty diabetic patients with carpal tunnel syndrome were split up into two groups. One received physiotherapy modality and the other received manual therapy. “Paired t-test revealed that all of the outcome measures had a significant change in the manual therapy group, whereas only the VAS and SSS changed significantly in the modality group at the end of 4 weeks. Independent t-test showed that the variables of SSS, FSS and MNT in the manual therapy group improved significantly greater than the modality group.”

Source: https://pubmed.ncbi.nlm.nih.gov/30197774/

Example 3: Omega-3 fatty acids decreased irritability of patients with bipolar disorder in an add-on, open label study

“The initial mean was 63.51 (SD 34.17), indicating that on average, subjects were irritable for about six of the previous ten days. The mean for the last recorded percentage was less than half of the initial score: 30.27 (SD 34.03). The decrease was found to be statistically significant using a paired sample t-test (t = 4.36, 36 df, p < .001).”

Source: https://nutritionj.biomedcentral.com/articles/10.1186/1475-2891-4-6

Example 4: Evaluating the Efficacy of COVID-19 Vaccines

“We reduced all values of vaccine efficacy by 30% to reflect the waning of vaccine efficacy against each endpoint over time. We tested the null hypothesis that the vaccine efficacy is 0% versus the alternative hypothesis that the vaccine efficacy is greater than 0% at the nominal significance level of 2.5%.”

Source: https://www.medrxiv.org/content/10.1101/2020.10.02.20205906v2.full

Example 5: Social Isolation During COVID-19 Pandemic. Perceived Stress and Containment Measures Compliance Among Polish and Italian Residents

“The Polish group had a higher stress level than the Italian group (mean PSS-10 total score 22,14 vs 17,01, respectively; p < 0.01). There was a greater prevalence of chronic diseases among Polish respondents. Italian subjects expressed more concern about their health, as well as about their future employment. Italian subjects did not comply with suggested restrictions as much as Polish subjects and were less eager to restrain from their usual activities (social, physical, and religious), which were more often perceived as “most needed matters” in Italian than in Polish residents.”

Source: https://www.frontiersin.org/articles/10.3389/fpsyg.2021.673514/full

Example 6: A Comparative Analysis of Student Performance in an Online vs. Face-to-Face Environmental Science Course From 2009 to 2016

“The independent sample t-test showed no significant difference in student performance between online and F2F learners with respect to gender [t(145) = 1.42, p = 0.122].”

Source: https://www.frontiersin.org/articles/10.3389/fcomp.2019.00007/full

But what does it all mean?

That’s what comes next. The examples above span a variety of different types of hypothesis tests. Within this chapter we will take a look at some of the terminology, formulas, and concepts related to Hypothesis Testing for 1 Sample.

Key Terminology and Formulas

Hypothesis: This is a claim or statement about a population, usually focusing on a parameter such as a proportion (%), mean, standard deviation, or variance. We will be focusing primarily on the proportion and the mean.

Hypothesis Test: Also known as a Significance Test or Test of Significance , the hypothesis test is the collection of procedures we use to test a claim about a population.

Null Hypothesis: This is a statement that the population parameter (such as the proportion, mean, standard deviation, or variance) is equal to some value. In simpler terms, the Null Hypothesis is a statement that “nothing is different from what usually happens.” The Null Hypothesis is usually denoted by [latex]H_{0}[/latex], followed by other symbols and notation that describe how the parameter is the same as some value.

Alternative Hypothesis: This is a statement that the population parameter (such as the proportion, mean, standard deviation, or variance) is somehow different the value involved in the Null Hypothesis. For our examples, “somehow different” will involve the use of [latex] [/latex], or [latex]\neq[/latex]. In simpler terms, the Alternative Hypothesis is a statement that “something is different from what usually happens.” The Alternative Hypothesis is usually denoted by [latex]H_{1}[/latex], [latex]H_{A}[/latex], or [latex]H_{a}[/latex], followed by other symbols and notation that describe how the parameter is different from some value.

Significance Level: We previous learned about the significance level as the “left over” stuff from the confidence level. This is still true, but we will now focus more on the significance level as its own value, and we will use the symbol alpha, [latex]\alpha[/latex]. This looks like a lowercase “a,” or a drawing of a little fish. The significance level [latex]\alpha[/latex] is the probability of rejecting the null hypothesis when it is actually true (more on what this means in the next section). The common values are still similar to what we had previously, 1%, 5%, and 10%. We commonly write these as decimals instead, 0.01, 0.05, and 0.10.

Test Statistic: One of the key components of a hypothesis test is what we call a test statistic . This is a calculation, sort of like a z-score, that is specific to the type of test being conducted. The idea behind a test statistic, relating it back to science projects, would be like calculations from measurements that were taken. In this chapter we will address the test statistic for 1 proportion, 1 mean when we know [latex]\sigma[/latex], and 1 mean with [latex]\sigma[/latex] unknown. The formulas are listed in the table below:

Critical Region: The critical region , also known as the rejection region , is the area in the normal (or other) distribution in which we reject the null hypothesis. Think of the critical region like a target area that you are aiming for. If we are able to get a value in this region, it means we have evidence for the claim.

Critical Value: These are like special z-scores for us; the critical value (or values, sometimes there are two) separates the critical region from the rest of the distribution. This is the non-target part, or what we are not aiming for. If our value is in this region, we do not have evidence for the claim.

P-Value: This is a special value that we compute. If we assume the null hypothesis is true, the p-value represents the probability that a test statistic is at least as extreme as the one we computed from our sample data; for us the test statistics would be either [latex]z[/latex] or [latex]t[/latex].

Decision Rule for Hypothesis Testing: There are a few ways we can arrive at our decision with a hypothesis test. We can arrive at our conclusion by using confidence intervals, critical values (also known as traditional method), and using p-values. Relating this to a science project, the decision rule would be what we take into consideration to arrive at our conclusion. When we make our decision, the wording will sound a little strange. We’ll say things like “we have enough evidence to reject the null hypothesis” or “there is insufficient evidence to reject the null hypothesis.”

Decision Rule with Critical Values: If the test statistic is in the critical region, we have enough evidence to reject the null hypothesis. We can also say we have sufficient evidence to support the claim. If the test statistic is not in the critical region, we fail to reject the null hypothesis. We can also say we do not have sufficient evidence to support the claim.

Decision Rule with P-Values: If the p-value is less than or equal to the significance level, we have enough evidence to reject the null hypothesis. We can also say we have sufficient evidence to support the claim. If the p-value is greater than the significance level, we fail to reject the null hypothesis. We can also say we do not have sufficient evidence to support the claim.

More About Hypotheses

Writing the Null and Alternative Hypothesis can be tricky. Here are a few examples of claims followed by the respective hypotheses:

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8.1: The Elements of Hypothesis Testing

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Learning Objectives

To understand the logical framework of tests of hypotheses.
To learn basic terminology connected with hypothesis testing.
To learn fundamental facts about hypothesis testing.

Types of Hypotheses

A hypothesis about the value of a population parameter is an assertion about its value. As in the introductory example we will be concerned with testing the truth of two competing hypotheses, only one of which can be true.

Definition: null hypothesis and alternative hypothesis

The null hypothesis , denoted $H_0$, is the statement about the population parameter that is assumed to be true unless there is convincing evidence to the contrary.
The alternative hypothesis , denoted $H_a$, is a statement about the population parameter that is contradictory to the null hypothesis, and is accepted as true only if there is convincing evidence in favor of it.

Definition: statistical procedure

Hypothesis testing is a statistical procedure in which a choice is made between a null hypothesis and an alternative hypothesis based on information in a sample.

The end result of a hypotheses testing procedure is a choice of one of the following two possible conclusions:

Reject $H_0$ (and therefore accept $H_a$), or
Fail to reject $H_0$ (and therefore fail to accept $H_a$).

The null hypothesis typically represents the status quo, or what has historically been true. In the example of the respirators, we would believe the claim of the manufacturer unless there is reason not to do so, so the null hypotheses is $H_0:\mu =75$. The alternative hypothesis in the example is the contradictory statement $H_a:\mu <75$. The null hypothesis will always be an assertion containing an equals sign, but depending on the situation the alternative hypothesis can have any one of three forms: with the symbol $<$, as in the example just discussed, with the symbol $>$, or with the symbol $\neq$. The following two examples illustrate the latter two cases.

Example $\PageIndex{1}$

A publisher of college textbooks claims that the average price of all hardbound college textbooks is $\$127.50$. A student group believes that the actual mean is higher and wishes to test their belief. State the relevant null and alternative hypotheses.

The default option is to accept the publisher’s claim unless there is compelling evidence to the contrary. Thus the null hypothesis is $H_0:\mu =127.50$. Since the student group thinks that the average textbook price is greater than the publisher’s figure, the alternative hypothesis in this situation is $H_a:\mu >127.50$.

Example $\PageIndex{2}$

The recipe for a bakery item is designed to result in a product that contains $8$ grams of fat per serving. The quality control department samples the product periodically to insure that the production process is working as designed. State the relevant null and alternative hypotheses.

The default option is to assume that the product contains the amount of fat it was formulated to contain unless there is compelling evidence to the contrary. Thus the null hypothesis is $H_0:\mu =8.0$. Since to contain either more fat than desired or to contain less fat than desired are both an indication of a faulty production process, the alternative hypothesis in this situation is that the mean is different from $8.0$, so $H_a:\mu \neq 8.0$.

In Example $\PageIndex{1}$, the textbook example, it might seem more natural that the publisher’s claim be that the average price is at most $\$127.50$, not exactly $\$127.50$. If the claim were made this way, then the null hypothesis would be $H_0:\mu \leq 127.50$, and the value $\$127.50$ given in the example would be the one that is least favorable to the publisher’s claim, the null hypothesis. It is always true that if the null hypothesis is retained for its least favorable value, then it is retained for every other value.

Thus in order to make the null and alternative hypotheses easy for the student to distinguish, in every example and problem in this text we will always present one of the two competing claims about the value of a parameter with an equality. The claim expressed with an equality is the null hypothesis. This is the same as always stating the null hypothesis in the least favorable light. So in the introductory example about the respirators, we stated the manufacturer’s claim as “the average is $75$ minutes” instead of the perhaps more natural “the average is at least $75$ minutes,” essentially reducing the presentation of the null hypothesis to its worst case.

The first step in hypothesis testing is to identify the null and alternative hypotheses.

The Logic of Hypothesis Testing

Although we will study hypothesis testing in situations other than for a single population mean (for example, for a population proportion instead of a mean or in comparing the means of two different populations), in this section the discussion will always be given in terms of a single population mean $\mu$.

The null hypothesis always has the form $H_0:\mu =\mu _0$ for a specific number $\mu _0$ (in the respirator example $\mu _0=75$, in the textbook example $\mu _0=127.50$, and in the baked goods example $\mu _0=8.0$). Since the null hypothesis is accepted unless there is strong evidence to the contrary, the test procedure is based on the initial assumption that $H_0$ is true. This point is so important that we will repeat it in a display:

The test procedure is based on the initial assumption that $H_0$ is true.

The criterion for judging between $H_0$ and $H_a$ based on the sample data is: if the value of $\overline{X}$ would be highly unlikely to occur if $H_0$ were true, but favors the truth of $H_a$, then we reject $H_0$ in favor of $H_a$. Otherwise we do not reject $H_0$.

Supposing for now that $\overline{X}$ follows a normal distribution, when the null hypothesis is true the density function for the sample mean $\overline{X}$ must be as in Figure $\PageIndex{1}$: a bell curve centered at $\mu _0$. Thus if $H_0$ is true then $\overline{X}$ is likely to take a value near $\mu _0$ and is unlikely to take values far away. Our decision procedure therefore reduces simply to:

if $H_a$ has the form $H_a:\mu <\mu _0$ then reject $H_0$ if $\bar{x}$ is far to the left of $\mu _0$;
if $H_a$ has the form $H_a:\mu >\mu _0$ then reject $H_0$ if $\bar{x}$ is far to the right of $\mu _0$;
if $H_a$ has the form $H_a:\mu \neq \mu _0$ then reject $H_0$ if $\bar{x}$ is far away from $\mu _0$ in either direction.

Think of the respirator example, for which the null hypothesis is $H_0:\mu =75$, the claim that the average time air is delivered for all respirators is $75$ minutes. If the sample mean is $75$ or greater then we certainly would not reject $H_0$ (since there is no issue with an emergency respirator delivering air even longer than claimed).

If the sample mean is slightly less than $75$ then we would logically attribute the difference to sampling error and also not reject $H_0$ either.

Values of the sample mean that are smaller and smaller are less and less likely to come from a population for which the population mean is $75$. Thus if the sample mean is far less than $75$, say around $60$ minutes or less, then we would certainly reject $H_0$, because we know that it is highly unlikely that the average of a sample would be so low if the population mean were $75$. This is the rare event criterion for rejection: what we actually observed $(\overline{X}<60)$ would be so rare an event if $\mu =75$ were true that we regard it as much more likely that the alternative hypothesis $\mu <75$ holds.

In summary, to decide between $H_0$ and $H_a$ in this example we would select a “rejection region” of values sufficiently far to the left of $75$, based on the rare event criterion, and reject $H_0$ if the sample mean $\overline{X}$ lies in the rejection region, but not reject $H_0$ if it does not.

The Rejection Region

Each different form of the alternative hypothesis Ha has its own kind of rejection region:

if (as in the respirator example) $H_a$ has the form $H_a:\mu <\mu _0$, we reject $H_0$ if $\bar{x}$ is far to the left of $\mu _0$, that is, to the left of some number $C$, so the rejection region has the form of an interval $(-\infty ,C]$;
if (as in the textbook example) $H_a$ has the form $H_a:\mu >\mu _0$, we reject $H_0$ if $\bar{x}$ is far to the right of $\mu _0$, that is, to the right of some number $C$, so the rejection region has the form of an interval $[C,\infty )$;
if (as in the baked good example) $H_a$ has the form $H_a:\mu \neq \mu _0$, we reject $H_0$ if $\bar{x}$ is far away from $\mu _0$ in either direction, that is, either to the left of some number $C$ or to the right of some other number $C′$, so the rejection region has the form of the union of two intervals $(-\infty ,C]\cup [C',\infty )$.

The key issue in our line of reasoning is the question of how to determine the number $C$ or numbers $C$ and $C′$, called the critical value or critical values of the statistic, that determine the rejection region.

Definition: critical values

The critical value or critical values of a test of hypotheses are the number or numbers that determine the rejection region.

Suppose the rejection region is a single interval, so we need to select a single number $C$. Here is the procedure for doing so. We select a small probability, denoted $\alpha$, say $1\%$, which we take as our definition of “rare event:” an event is “rare” if its probability of occurrence is less than $\alpha$. (In all the examples and problems in this text the value of $\alpha$ will be given already.) The probability that $\overline{X}$ takes a value in an interval is the area under its density curve and above that interval, so as shown in Figure $\PageIndex{2}$ (drawn under the assumption that $H_0$ is true, so that the curve centers at $\mu _0$) the critical value $C$ is the value of $\overline{X}$ that cuts off a tail area $\alpha$ in the probability density curve of $\overline{X}$. When the rejection region is in two pieces, that is, composed of two intervals, the total area above both of them must be $\alpha$, so the area above each one is $\alpha /2$, as also shown in Figure $\PageIndex{2}$.

The number $\alpha$ is the total area of a tail or a pair of tails.

Example $\PageIndex{3}$

In the context of Example $\PageIndex{2}$, suppose that it is known that the population is normally distributed with standard deviation $\alpha =0.15$ gram, and suppose that the test of hypotheses $H_0:\mu =8.0$ versus $H_a:\mu \neq 8.0$ will be performed with a sample of size $5$. Construct the rejection region for the test for the choice $\alpha =0.10$. Explain the decision procedure and interpret it.

If $H_0$ is true then the sample mean $\overline{X}$ is normally distributed with mean and standard deviation

\[\begin{align} \mu _{\overline{X}} &=\mu \nonumber \\[5pt] &=8.0 \nonumber \end{align} \nonumber \]

\[\begin{align} \sigma _{\overline{X}}&=\dfrac{\sigma}{\sqrt{n}} \nonumber \\[5pt] &= \dfrac{0.15}{\sqrt{5}} \nonumber\\[5pt] &=0.067 \nonumber \end{align} \nonumber \]

Since $H_a$ contains the $\neq$ symbol the rejection region will be in two pieces, each one corresponding to a tail of area $\alpha /2=0.10/2=0.05$. From Figure 7.1.6, $z_{0.05}=1.645$, so $C$ and $C′$ are $1.645$ standard deviations of $\overline{X}$ to the right and left of its mean $8.0$:

\[C=8.0-(1.645)(0.067) = 7.89 \; \; \text{and}\; \; C'=8.0 + (1.645)(0.067) = 8.11 \nonumber \]

The result is shown in Figure $\PageIndex{3}$. α = 0.1

The decision procedure is: take a sample of size $5$ and compute the sample mean $\bar{x}$. If $\bar{x}$ is either $7.89$ grams or less or $8.11$ grams or more then reject the hypothesis that the average amount of fat in all servings of the product is $8.0$ grams in favor of the alternative that it is different from $8.0$ grams. Otherwise do not reject the hypothesis that the average amount is $8.0$ grams.

The reasoning is that if the true average amount of fat per serving were $8.0$ grams then there would be less than a $10\%$ chance that a sample of size $5$ would produce a mean of either $7.89$ grams or less or $8.11$ grams or more. Hence if that happened it would be more likely that the value $8.0$ is incorrect (always assuming that the population standard deviation is $0.15$ gram).

Because the rejection regions are computed based on areas in tails of distributions, as shown in Figure $\PageIndex{2}$, hypothesis tests are classified according to the form of the alternative hypothesis in the following way.

Definitions: Test classifications

If $H_a$ has the form $\mu \neq \mu _0$ the test is called a two-tailed test .
If $H_a$ has the form $\mu < \mu _0$ the test is called a left-tailed test .
If $H_a$ has the form $\mu > \mu _0$the test is called a right-tailed test .

Each of the last two forms is also called a one-tailed test .

Two Types of Errors

The format of the testing procedure in general terms is to take a sample and use the information it contains to come to a decision about the two hypotheses. As stated before our decision will always be either

reject the null hypothesis $H_0$ in favor of the alternative $H_a$ presented, or
do not reject the null hypothesis $H_0$ in favor of the alternative $H_0$ presented.

There are four possible outcomes of hypothesis testing procedure, as shown in the following table:

As the table shows, there are two ways to be right and two ways to be wrong. Typically to reject $H_0$ when it is actually true is a more serious error than to fail to reject it when it is false, so the former error is labeled “ Type I ” and the latter error “ Type II ”.

Definition: Type I and Type II errors

In a test of hypotheses:

A Type I error is the decision to reject $H_0$ when it is in fact true.
A Type II error is the decision not to reject $H_0$ when it is in fact not true.

Unless we perform a census we do not have certain knowledge, so we do not know whether our decision matches the true state of nature or if we have made an error. We reject $H_0$ if what we observe would be a “rare” event if $H_0$ were true. But rare events are not impossible: they occur with probability $\alpha$. Thus when $H_0$ is true, a rare event will be observed in the proportion $\alpha$ of repeated similar tests, and $H_0$ will be erroneously rejected in those tests. Thus $\alpha$ is the probability that in following the testing procedure to decide between $H_0$ and $H_a$ we will make a Type I error.

Definition: level of significance

The number $\alpha$ that is used to determine the rejection region is called the level of significance of the test. It is the probability that the test procedure will result in a Type I error .

The probability of making a Type II error is too complicated to discuss in a beginning text, so we will say no more about it than this: for a fixed sample size, choosing $alpha$ smaller in order to reduce the chance of making a Type I error has the effect of increasing the chance of making a Type II error . The only way to simultaneously reduce the chances of making either kind of error is to increase the sample size.

Standardizing the Test Statistic

Hypotheses testing will be considered in a number of contexts, and great unification as well as simplification results when the relevant sample statistic is standardized by subtracting its mean from it and then dividing by its standard deviation. The resulting statistic is called a standardized test statistic . In every situation treated in this and the following two chapters the standardized test statistic will have either the standard normal distribution or Student’s $t$-distribution.

Definition: hypothesis test

A standardized test statistic for a hypothesis test is the statistic that is formed by subtracting from the statistic of interest its mean and dividing by its standard deviation.

For example, reviewing Example $\PageIndex{3}$, if instead of working with the sample mean $\overline{X}$ we instead work with the test statistic

\[\frac{\overline{X}-8.0}{0.067} \nonumber \]

then the distribution involved is standard normal and the critical values are just $\pm z_{0.05}$. The extra work that was done to find that $C=7.89$ and $C′=8.11$ is eliminated. In every hypothesis test in this book the standardized test statistic will be governed by either the standard normal distribution or Student’s $t$-distribution. Information about rejection regions is summarized in the following tables:

Every instance of hypothesis testing discussed in this and the following two chapters will have a rejection region like one of the six forms tabulated in the tables above.

No matter what the context a test of hypotheses can always be performed by applying the following systematic procedure, which will be illustrated in the examples in the succeeding sections.

Systematic Hypothesis Testing Procedure: Critical Value Approach

Identify the null and alternative hypotheses.
Identify the relevant test statistic and its distribution.
Compute from the data the value of the test statistic.
Construct the rejection region.
Compare the value computed in Step 3 to the rejection region constructed in Step 4 and make a decision. Formulate the decision in the context of the problem, if applicable.

The procedure that we have outlined in this section is called the “Critical Value Approach” to hypothesis testing to distinguish it from an alternative but equivalent approach that will be introduced at the end of Section 8.3.

Key Takeaway

A test of hypotheses is a statistical process for deciding between two competing assertions about a population parameter.
The testing procedure is formalized in a five-step procedure.

Hypothesis Testing

Hypothesis testing is a tool for making statistical inferences about the population data. It is an analysis tool that tests assumptions and determines how likely something is within a given standard of accuracy. Hypothesis testing provides a way to verify whether the results of an experiment are valid.

A null hypothesis and an alternative hypothesis are set up before performing the hypothesis testing. This helps to arrive at a conclusion regarding the sample obtained from the population. In this article, we will learn more about hypothesis testing, its types, steps to perform the testing, and associated examples.

What is Hypothesis Testing in Statistics?

Hypothesis testing uses sample data from the population to draw useful conclusions regarding the population probability distribution . It tests an assumption made about the data using different types of hypothesis testing methodologies. The hypothesis testing results in either rejecting or not rejecting the null hypothesis.

Hypothesis Testing Definition

Hypothesis testing can be defined as a statistical tool that is used to identify if the results of an experiment are meaningful or not. It involves setting up a null hypothesis and an alternative hypothesis. These two hypotheses will always be mutually exclusive. This means that if the null hypothesis is true then the alternative hypothesis is false and vice versa. An example of hypothesis testing is setting up a test to check if a new medicine works on a disease in a more efficient manner.

Null Hypothesis

The null hypothesis is a concise mathematical statement that is used to indicate that there is no difference between two possibilities. In other words, there is no difference between certain characteristics of data. This hypothesis assumes that the outcomes of an experiment are based on chance alone. It is denoted as $H_{0}$. Hypothesis testing is used to conclude if the null hypothesis can be rejected or not. Suppose an experiment is conducted to check if girls are shorter than boys at the age of 5. The null hypothesis will say that they are the same height.

Alternative Hypothesis

The alternative hypothesis is an alternative to the null hypothesis. It is used to show that the observations of an experiment are due to some real effect. It indicates that there is a statistical significance between two possible outcomes and can be denoted as $H_{1}$ or $H_{a}$. For the above-mentioned example, the alternative hypothesis would be that girls are shorter than boys at the age of 5.

Hypothesis Testing P Value

In hypothesis testing, the p value is used to indicate whether the results obtained after conducting a test are statistically significant or not. It also indicates the probability of making an error in rejecting or not rejecting the null hypothesis.This value is always a number between 0 and 1. The p value is compared to an alpha level, $\alpha$ or significance level. The alpha level can be defined as the acceptable risk of incorrectly rejecting the null hypothesis. The alpha level is usually chosen between 1% to 5%.

Hypothesis Testing Critical region

All sets of values that lead to rejecting the null hypothesis lie in the critical region. Furthermore, the value that separates the critical region from the non-critical region is known as the critical value.

Hypothesis Testing Formula

Depending upon the type of data available and the size, different types of hypothesis testing are used to determine whether the null hypothesis can be rejected or not. The hypothesis testing formula for some important test statistics are given below:

z = $\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}$. $\overline{x}$ is the sample mean, $\mu$ is the population mean, $\sigma$ is the population standard deviation and n is the size of the sample.
t = $\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}$. s is the sample standard deviation.
$\chi ^{2} = \sum \frac{(O_{i}-E_{i})^{2}}{E_{i}}$. $O_{i}$ is the observed value and $E_{i}$ is the expected value.

We will learn more about these test statistics in the upcoming section.

Types of Hypothesis Testing

Selecting the correct test for performing hypothesis testing can be confusing. These tests are used to determine a test statistic on the basis of which the null hypothesis can either be rejected or not rejected. Some of the important tests used for hypothesis testing are given below.

Hypothesis Testing Z Test

A z test is a way of hypothesis testing that is used for a large sample size (n ≥ 30). It is used to determine whether there is a difference between the population mean and the sample mean when the population standard deviation is known. It can also be used to compare the mean of two samples. It is used to compute the z test statistic. The formulas are given as follows:

One sample: z = $\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}$.
Two samples: z = $\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}$.

Hypothesis Testing t Test

The t test is another method of hypothesis testing that is used for a small sample size (n < 30). It is also used to compare the sample mean and population mean. However, the population standard deviation is not known. Instead, the sample standard deviation is known. The mean of two samples can also be compared using the t test.

One sample: t = $\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}$.
Two samples: t = $\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{s_{1}^{2}}{n_{1}}+\frac{s_{2}^{2}}{n_{2}}}}$.

Hypothesis Testing Chi Square

The Chi square test is a hypothesis testing method that is used to check whether the variables in a population are independent or not. It is used when the test statistic is chi-squared distributed.

One Tailed Hypothesis Testing

One tailed hypothesis testing is done when the rejection region is only in one direction. It can also be known as directional hypothesis testing because the effects can be tested in one direction only. This type of testing is further classified into the right tailed test and left tailed test.

Right Tailed Hypothesis Testing

The right tail test is also known as the upper tail test. This test is used to check whether the population parameter is greater than some value. The null and alternative hypotheses for this test are given as follows:

$H_{0}$: The population parameter is ≤ some value

$H_{1}$: The population parameter is > some value.

If the test statistic has a greater value than the critical value then the null hypothesis is rejected

Left Tailed Hypothesis Testing

The left tail test is also known as the lower tail test. It is used to check whether the population parameter is less than some value. The hypotheses for this hypothesis testing can be written as follows:

$H_{0}$: The population parameter is ≥ some value

$H_{1}$: The population parameter is < some value.

The null hypothesis is rejected if the test statistic has a value lesser than the critical value.

Two Tailed Hypothesis Testing

In this hypothesis testing method, the critical region lies on both sides of the sampling distribution. It is also known as a non - directional hypothesis testing method. The two-tailed test is used when it needs to be determined if the population parameter is assumed to be different than some value. The hypotheses can be set up as follows:

$H_{0}$: the population parameter = some value

$H_{1}$: the population parameter ≠ some value

The null hypothesis is rejected if the test statistic has a value that is not equal to the critical value.

Hypothesis Testing Steps

Hypothesis testing can be easily performed in five simple steps. The most important step is to correctly set up the hypotheses and identify the right method for hypothesis testing. The basic steps to perform hypothesis testing are as follows:

Step 1: Set up the null hypothesis by correctly identifying whether it is the left-tailed, right-tailed, or two-tailed hypothesis testing.
Step 2: Set up the alternative hypothesis.
Step 3: Choose the correct significance level, $\alpha$, and find the critical value.
Step 4: Calculate the correct test statistic (z, t or $\chi$) and p-value.
Step 5: Compare the test statistic with the critical value or compare the p-value with $\alpha$ to arrive at a conclusion. In other words, decide if the null hypothesis is to be rejected or not.

Hypothesis Testing Example

The best way to solve a problem on hypothesis testing is by applying the 5 steps mentioned in the previous section. Suppose a researcher claims that the mean average weight of men is greater than 100kgs with a standard deviation of 15kgs. 30 men are chosen with an average weight of 112.5 Kgs. Using hypothesis testing, check if there is enough evidence to support the researcher's claim. The confidence interval is given as 95%.

Step 1: This is an example of a right-tailed test. Set up the null hypothesis as $H_{0}$: $\mu$ = 100.

Step 2: The alternative hypothesis is given by $H_{1}$: $\mu$ > 100.

Step 3: As this is a one-tailed test, $\alpha$ = 100% - 95% = 5%. This can be used to determine the critical value.

1 - $\alpha$ = 1 - 0.05 = 0.95

0.95 gives the required area under the curve. Now using a normal distribution table, the area 0.95 is at z = 1.645. A similar process can be followed for a t-test. The only additional requirement is to calculate the degrees of freedom given by n - 1.

Step 4: Calculate the z test statistic. This is because the sample size is 30. Furthermore, the sample and population means are known along with the standard deviation.

z = $\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}$.

$\mu$ = 100, $\overline{x}$ = 112.5, n = 30, $\sigma$ = 15

z = $\frac{112.5-100}{\frac{15}{\sqrt{30}}}$ = 4.56

Step 5: Conclusion. As 4.56 > 1.645 thus, the null hypothesis can be rejected.

Hypothesis Testing and Confidence Intervals

Confidence intervals form an important part of hypothesis testing. This is because the alpha level can be determined from a given confidence interval. Suppose a confidence interval is given as 95%. Subtract the confidence interval from 100%. This gives 100 - 95 = 5% or 0.05. This is the alpha value of a one-tailed hypothesis testing. To obtain the alpha value for a two-tailed hypothesis testing, divide this value by 2. This gives 0.05 / 2 = 0.025.

Probability and Statistics
Data Handling

Important Notes on Hypothesis Testing

Hypothesis testing is a technique that is used to verify whether the results of an experiment are statistically significant.
It involves the setting up of a null hypothesis and an alternate hypothesis.
There are three types of tests that can be conducted under hypothesis testing - z test, t test, and chi square test.
Hypothesis testing can be classified as right tail, left tail, and two tail tests.

Examples on Hypothesis Testing

Example 1: The average weight of a dumbbell in a gym is 90lbs. However, a physical trainer believes that the average weight might be higher. A random sample of 5 dumbbells with an average weight of 110lbs and a standard deviation of 18lbs. Using hypothesis testing check if the physical trainer's claim can be supported for a 95% confidence level. Solution: As the sample size is lesser than 30, the t-test is used. $H_{0}$: $\mu$ = 90, $H_{1}$: $\mu$ > 90 $\overline{x}$ = 110, $\mu$ = 90, n = 5, s = 18. $\alpha$ = 0.05 Using the t-distribution table, the critical value is 2.132 t = $\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}$ t = 2.484 As 2.484 > 2.132, the null hypothesis is rejected. Answer: The average weight of the dumbbells may be greater than 90lbs
Example 2: The average score on a test is 80 with a standard deviation of 10. With a new teaching curriculum introduced it is believed that this score will change. On random testing, the score of 38 students, the mean was found to be 88. With a 0.05 significance level, is there any evidence to support this claim? Solution: This is an example of two-tail hypothesis testing. The z test will be used. $H_{0}$: $\mu$ = 80, $H_{1}$: $\mu$ ≠ 80 $\overline{x}$ = 88, $\mu$ = 80, n = 36, $\sigma$ = 10. $\alpha$ = 0.05 / 2 = 0.025 The critical value using the normal distribution table is 1.96 z = $\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}$ z = $\frac{88-80}{\frac{10}{\sqrt{36}}}$ = 4.8 As 4.8 > 1.96, the null hypothesis is rejected. Answer: There is a difference in the scores after the new curriculum was introduced.
Example 3: The average score of a class is 90. However, a teacher believes that the average score might be lower. The scores of 6 students were randomly measured. The mean was 82 with a standard deviation of 18. With a 0.05 significance level use hypothesis testing to check if this claim is true. Solution: The t test will be used. $H_{0}$: $\mu$ = 90, $H_{1}$: $\mu$ < 90 $\overline{x}$ = 110, $\mu$ = 90, n = 6, s = 18 The critical value from the t table is -2.015 t = $\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}$ t = $\frac{82-90}{\frac{18}{\sqrt{6}}}$ t = -1.088 As -1.088 > -2.015, we fail to reject the null hypothesis. Answer: There is not enough evidence to support the claim.

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FAQs on Hypothesis Testing

What is hypothesis testing.

Hypothesis testing in statistics is a tool that is used to make inferences about the population data. It is also used to check if the results of an experiment are valid.

What is the z Test in Hypothesis Testing?

The z test in hypothesis testing is used to find the z test statistic for normally distributed data . The z test is used when the standard deviation of the population is known and the sample size is greater than or equal to 30.

What is the t Test in Hypothesis Testing?

The t test in hypothesis testing is used when the data follows a student t distribution . It is used when the sample size is less than 30 and standard deviation of the population is not known.

What is the formula for z test in Hypothesis Testing?

The formula for a one sample z test in hypothesis testing is z = $\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}$ and for two samples is z = $\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}$.

What is the p Value in Hypothesis Testing?

The p value helps to determine if the test results are statistically significant or not. In hypothesis testing, the null hypothesis can either be rejected or not rejected based on the comparison between the p value and the alpha level.

What is One Tail Hypothesis Testing?

When the rejection region is only on one side of the distribution curve then it is known as one tail hypothesis testing. The right tail test and the left tail test are two types of directional hypothesis testing.

What is the Alpha Level in Two Tail Hypothesis Testing?

To get the alpha level in a two tail hypothesis testing divide $\alpha$ by 2. This is done as there are two rejection regions in the curve.

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A Complete Guide on Hypothesis Testing in Statistics

In today’s data-driven world , decisions are based on data all the time. Hypothesis plays a crucial role in that process, whether it may be making business decisions, in the health sector, academia, or in quality improvement. Without hypothesis & hypothesis tests, you risk drawing the wrong conclusions and making bad decisions. In this tutorial, you will look at Hypothesis Testing in Statistics.

What Is Hypothesis Testing in Statistics?

Hypothesis Testing is a type of statistical analysis in which you put your assumptions about a population parameter to the test. It is used to estimate the relationship between 2 statistical variables.

Let's discuss few examples of statistical hypothesis from real-life -

A teacher assumes that 60% of his college's students come from lower-middle-class families.
A doctor believes that 3D (Diet, Dose, and Discipline) is 90% effective for diabetic patients.

Now that you know about hypothesis testing, look at the two types of hypothesis testing in statistics.

Hypothesis Testing Formula

Z = ( x̅ – μ0 ) / (σ /√n)

Here, x̅ is the sample mean,
μ0 is the population mean,
σ is the standard deviation,
n is the sample size.

How Hypothesis Testing Works?

An analyst performs hypothesis testing on a statistical sample to present evidence of the plausibility of the null hypothesis. Measurements and analyses are conducted on a random sample of the population to test a theory. Analysts use a random population sample to test two hypotheses: the null and alternative hypotheses.

The null hypothesis is typically an equality hypothesis between population parameters; for example, a null hypothesis may claim that the population means return equals zero. The alternate hypothesis is essentially the inverse of the null hypothesis (e.g., the population means the return is not equal to zero). As a result, they are mutually exclusive, and only one can be correct. One of the two possibilities, however, will always be correct.

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Null Hypothesis and Alternate Hypothesis

The Null Hypothesis is the assumption that the event will not occur. A null hypothesis has no bearing on the study's outcome unless it is rejected.

H0 is the symbol for it, and it is pronounced H-naught.

The Alternate Hypothesis is the logical opposite of the null hypothesis. The acceptance of the alternative hypothesis follows the rejection of the null hypothesis. H1 is the symbol for it.

Let's understand this with an example.

A sanitizer manufacturer claims that its product kills 95 percent of germs on average.

To put this company's claim to the test, create a null and alternate hypothesis.

H0 (Null Hypothesis): Average = 95%.

Alternative Hypothesis (H1): The average is less than 95%.

Another straightforward example to understand this concept is determining whether or not a coin is fair and balanced. The null hypothesis states that the probability of a show of heads is equal to the likelihood of a show of tails. In contrast, the alternate theory states that the probability of a show of heads and tails would be very different.

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Hypothesis Testing Calculation With Examples

Let's consider a hypothesis test for the average height of women in the United States. Suppose our null hypothesis is that the average height is 5'4". We gather a sample of 100 women and determine that their average height is 5'5". The standard deviation of population is 2.

To calculate the z-score, we would use the following formula:

z = ( x̅ – μ0 ) / (σ /√n)

z = (5'5" - 5'4") / (2" / √100)

z = 0.5 / (0.045)

We will reject the null hypothesis as the z-score of 11.11 is very large and conclude that there is evidence to suggest that the average height of women in the US is greater than 5'4".

Steps of Hypothesis Testing

Step 1: specify your null and alternate hypotheses.

It is critical to rephrase your original research hypothesis (the prediction that you wish to study) as a null (Ho) and alternative (Ha) hypothesis so that you can test it quantitatively. Your first hypothesis, which predicts a link between variables, is generally your alternate hypothesis. The null hypothesis predicts no link between the variables of interest.

Step 2: Gather Data

For a statistical test to be legitimate, sampling and data collection must be done in a way that is meant to test your hypothesis. You cannot draw statistical conclusions about the population you are interested in if your data is not representative.

Step 3: Conduct a Statistical Test

Other statistical tests are available, but they all compare within-group variance (how to spread out the data inside a category) against between-group variance (how different the categories are from one another). If the between-group variation is big enough that there is little or no overlap between groups, your statistical test will display a low p-value to represent this. This suggests that the disparities between these groups are unlikely to have occurred by accident. Alternatively, if there is a large within-group variance and a low between-group variance, your statistical test will show a high p-value. Any difference you find across groups is most likely attributable to chance. The variety of variables and the level of measurement of your obtained data will influence your statistical test selection.

Step 4: Determine Rejection Of Your Null Hypothesis

Your statistical test results must determine whether your null hypothesis should be rejected or not. In most circumstances, you will base your judgment on the p-value provided by the statistical test. In most circumstances, your preset level of significance for rejecting the null hypothesis will be 0.05 - that is, when there is less than a 5% likelihood that these data would be seen if the null hypothesis were true. In other circumstances, researchers use a lower level of significance, such as 0.01 (1%). This reduces the possibility of wrongly rejecting the null hypothesis.

Step 5: Present Your Results

The findings of hypothesis testing will be discussed in the results and discussion portions of your research paper, dissertation, or thesis. You should include a concise overview of the data and a summary of the findings of your statistical test in the results section. You can talk about whether your results confirmed your initial hypothesis or not in the conversation. Rejecting or failing to reject the null hypothesis is a formal term used in hypothesis testing. This is likely a must for your statistics assignments.

Types of Hypothesis Testing

To determine whether a discovery or relationship is statistically significant, hypothesis testing uses a z-test. It usually checks to see if two means are the same (the null hypothesis). Only when the population standard deviation is known and the sample size is 30 data points or more, can a z-test be applied.

A statistical test called a t-test is employed to compare the means of two groups. To determine whether two groups differ or if a procedure or treatment affects the population of interest, it is frequently used in hypothesis testing.

Chi-Square

You utilize a Chi-square test for hypothesis testing concerning whether your data is as predicted. To determine if the expected and observed results are well-fitted, the Chi-square test analyzes the differences between categorical variables from a random sample. The test's fundamental premise is that the observed values in your data should be compared to the predicted values that would be present if the null hypothesis were true.

Hypothesis Testing and Confidence Intervals

Both confidence intervals and hypothesis tests are inferential techniques that depend on approximating the sample distribution. Data from a sample is used to estimate a population parameter using confidence intervals. Data from a sample is used in hypothesis testing to examine a given hypothesis. We must have a postulated parameter to conduct hypothesis testing.

Bootstrap distributions and randomization distributions are created using comparable simulation techniques. The observed sample statistic is the focal point of a bootstrap distribution, whereas the null hypothesis value is the focal point of a randomization distribution.

A variety of feasible population parameter estimates are included in confidence ranges. In this lesson, we created just two-tailed confidence intervals. There is a direct connection between these two-tail confidence intervals and these two-tail hypothesis tests. The results of a two-tailed hypothesis test and two-tailed confidence intervals typically provide the same results. In other words, a hypothesis test at the 0.05 level will virtually always fail to reject the null hypothesis if the 95% confidence interval contains the predicted value. A hypothesis test at the 0.05 level will nearly certainly reject the null hypothesis if the 95% confidence interval does not include the hypothesized parameter.

Simple and Composite Hypothesis Testing

Depending on the population distribution, you can classify the statistical hypothesis into two types.

Simple Hypothesis: A simple hypothesis specifies an exact value for the parameter.

Composite Hypothesis: A composite hypothesis specifies a range of values.

A company is claiming that their average sales for this quarter are 1000 units. This is an example of a simple hypothesis.

Suppose the company claims that the sales are in the range of 900 to 1000 units. Then this is a case of a composite hypothesis.

One-Tailed and Two-Tailed Hypothesis Testing

The One-Tailed test, also called a directional test, considers a critical region of data that would result in the null hypothesis being rejected if the test sample falls into it, inevitably meaning the acceptance of the alternate hypothesis.

In a one-tailed test, the critical distribution area is one-sided, meaning the test sample is either greater or lesser than a specific value.

In two tails, the test sample is checked to be greater or less than a range of values in a Two-Tailed test, implying that the critical distribution area is two-sided.

If the sample falls within this range, the alternate hypothesis will be accepted, and the null hypothesis will be rejected.

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Right Tailed Hypothesis Testing

If the larger than (>) sign appears in your hypothesis statement, you are using a right-tailed test, also known as an upper test. Or, to put it another way, the disparity is to the right. For instance, you can contrast the battery life before and after a change in production. Your hypothesis statements can be the following if you want to know if the battery life is longer than the original (let's say 90 hours):

The null hypothesis is (H0 <= 90) or less change.
A possibility is that battery life has risen (H1) > 90.

The crucial point in this situation is that the alternate hypothesis (H1), not the null hypothesis, decides whether you get a right-tailed test.

Left Tailed Hypothesis Testing

Alternative hypotheses that assert the true value of a parameter is lower than the null hypothesis are tested with a left-tailed test; they are indicated by the asterisk "<".

Suppose H0: mean = 50 and H1: mean not equal to 50

According to the H1, the mean can be greater than or less than 50. This is an example of a Two-tailed test.

In a similar manner, if H0: mean >=50, then H1: mean <50

Here the mean is less than 50. It is called a One-tailed test.

Type 1 and Type 2 Error

A hypothesis test can result in two types of errors.

Type 1 Error: A Type-I error occurs when sample results reject the null hypothesis despite being true.

Type 2 Error: A Type-II error occurs when the null hypothesis is not rejected when it is false, unlike a Type-I error.

Suppose a teacher evaluates the examination paper to decide whether a student passes or fails.

H0: Student has passed

H1: Student has failed

Type I error will be the teacher failing the student [rejects H0] although the student scored the passing marks [H0 was true].

Type II error will be the case where the teacher passes the student [do not reject H0] although the student did not score the passing marks [H1 is true].

Level of Significance

The alpha value is a criterion for determining whether a test statistic is statistically significant. In a statistical test, Alpha represents an acceptable probability of a Type I error. Because alpha is a probability, it can be anywhere between 0 and 1. In practice, the most commonly used alpha values are 0.01, 0.05, and 0.1, which represent a 1%, 5%, and 10% chance of a Type I error, respectively (i.e. rejecting the null hypothesis when it is in fact correct).

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A p-value is a metric that expresses the likelihood that an observed difference could have occurred by chance. As the p-value decreases the statistical significance of the observed difference increases. If the p-value is too low, you reject the null hypothesis.

Here you have taken an example in which you are trying to test whether the new advertising campaign has increased the product's sales. The p-value is the likelihood that the null hypothesis, which states that there is no change in the sales due to the new advertising campaign, is true. If the p-value is .30, then there is a 30% chance that there is no increase or decrease in the product's sales. If the p-value is 0.03, then there is a 3% probability that there is no increase or decrease in the sales value due to the new advertising campaign. As you can see, the lower the p-value, the chances of the alternate hypothesis being true increases, which means that the new advertising campaign causes an increase or decrease in sales.

Why is Hypothesis Testing Important in Research Methodology?

Hypothesis testing is crucial in research methodology for several reasons:

Provides evidence-based conclusions: It allows researchers to make objective conclusions based on empirical data, providing evidence to support or refute their research hypotheses.
Supports decision-making: It helps make informed decisions, such as accepting or rejecting a new treatment, implementing policy changes, or adopting new practices.
Adds rigor and validity: It adds scientific rigor to research using statistical methods to analyze data, ensuring that conclusions are based on sound statistical evidence.
Contributes to the advancement of knowledge: By testing hypotheses, researchers contribute to the growth of knowledge in their respective fields by confirming existing theories or discovering new patterns and relationships.

Limitations of Hypothesis Testing

Hypothesis testing has some limitations that researchers should be aware of:

It cannot prove or establish the truth: Hypothesis testing provides evidence to support or reject a hypothesis, but it cannot confirm the absolute truth of the research question.
Results are sample-specific: Hypothesis testing is based on analyzing a sample from a population, and the conclusions drawn are specific to that particular sample.
Possible errors: During hypothesis testing, there is a chance of committing type I error (rejecting a true null hypothesis) or type II error (failing to reject a false null hypothesis).
Assumptions and requirements: Different tests have specific assumptions and requirements that must be met to accurately interpret results.

After reading this tutorial, you would have a much better understanding of hypothesis testing, one of the most important concepts in the field of Data Science . The majority of hypotheses are based on speculation about observed behavior, natural phenomena, or established theories.

If you are interested in statistics of data science and skills needed for such a career, you ought to explore Simplilearn’s Post Graduate Program in Data Science.

If you have any questions regarding this ‘Hypothesis Testing In Statistics’ tutorial, do share them in the comment section. Our subject matter expert will respond to your queries. Happy learning!

1. What is hypothesis testing in statistics with example?

Hypothesis testing is a statistical method used to determine if there is enough evidence in a sample data to draw conclusions about a population. It involves formulating two competing hypotheses, the null hypothesis (H0) and the alternative hypothesis (Ha), and then collecting data to assess the evidence. An example: testing if a new drug improves patient recovery (Ha) compared to the standard treatment (H0) based on collected patient data.

2. What is hypothesis testing and its types?

Hypothesis testing is a statistical method used to make inferences about a population based on sample data. It involves formulating two hypotheses: the null hypothesis (H0), which represents the default assumption, and the alternative hypothesis (Ha), which contradicts H0. The goal is to assess the evidence and determine whether there is enough statistical significance to reject the null hypothesis in favor of the alternative hypothesis.

Types of hypothesis testing:

One-sample test: Used to compare a sample to a known value or a hypothesized value.
Two-sample test: Compares two independent samples to assess if there is a significant difference between their means or distributions.
Paired-sample test: Compares two related samples, such as pre-test and post-test data, to evaluate changes within the same subjects over time or under different conditions.
Chi-square test: Used to analyze categorical data and determine if there is a significant association between variables.
ANOVA (Analysis of Variance): Compares means across multiple groups to check if there is a significant difference between them.

3. What are the steps of hypothesis testing?

The steps of hypothesis testing are as follows:

Formulate the hypotheses: State the null hypothesis (H0) and the alternative hypothesis (Ha) based on the research question.
Set the significance level: Determine the acceptable level of error (alpha) for making a decision.
Collect and analyze data: Gather and process the sample data.
Compute test statistic: Calculate the appropriate statistical test to assess the evidence.
Make a decision: Compare the test statistic with critical values or p-values and determine whether to reject H0 in favor of Ha or not.
Draw conclusions: Interpret the results and communicate the findings in the context of the research question.

4. What are the 2 types of hypothesis testing?

One-tailed (or one-sided) test: Tests for the significance of an effect in only one direction, either positive or negative.
Two-tailed (or two-sided) test: Tests for the significance of an effect in both directions, allowing for the possibility of a positive or negative effect.

The choice between one-tailed and two-tailed tests depends on the specific research question and the directionality of the expected effect.

5. What are the 3 major types of hypothesis?

The three major types of hypotheses are:

Null Hypothesis (H0): Represents the default assumption, stating that there is no significant effect or relationship in the data.
Alternative Hypothesis (Ha): Contradicts the null hypothesis and proposes a specific effect or relationship that researchers want to investigate.
Nondirectional Hypothesis: An alternative hypothesis that doesn't specify the direction of the effect, leaving it open for both positive and negative possibilities.

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Avijeet is a Senior Research Analyst at Simplilearn. Passionate about Data Analytics, Machine Learning, and Deep Learning, Avijeet is also interested in politics, cricket, and football.

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1.4: Basic Concepts of Hypothesis Testing

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Page ID 1715

John H. McDonald
University of Delaware

Learning Objectives

One of the main goals of statistical hypothesis testing is to estimate the $P$ value, which is the probability of obtaining the observed results, or something more extreme, if the null hypothesis were true. If the observed results are unlikely under the null hypothesis, reject the null hypothesis.
Alternatives to this "frequentist" approach to statistics include Bayesian statistics and estimation of effect sizes and confidence intervals.

Introduction

There are different ways of doing statistics. The technique used by the vast majority of biologists, and the technique that most of this handbook describes, is sometimes called "frequentist" or "classical" statistics. It involves testing a null hypothesis by comparing the data you observe in your experiment with the predictions of a null hypothesis. You estimate what the probability would be of obtaining the observed results, or something more extreme, if the null hypothesis were true. If this estimated probability (the $P$ value) is small enough (below the significance value), then you conclude that it is unlikely that the null hypothesis is true; you reject the null hypothesis and accept an alternative hypothesis.

Many statisticians harshly criticize frequentist statistics, but their criticisms haven't had much effect on the way most biologists do statistics. Here I will outline some of the key concepts used in frequentist statistics, then briefly describe some of the alternatives.

Null Hypothesis

The null hypothesis is a statement that you want to test. In general, the null hypothesis is that things are the same as each other, or the same as a theoretical expectation. For example, if you measure the size of the feet of male and female chickens, the null hypothesis could be that the average foot size in male chickens is the same as the average foot size in female chickens. If you count the number of male and female chickens born to a set of hens, the null hypothesis could be that the ratio of males to females is equal to a theoretical expectation of a $1:1$ ratio.

The alternative hypothesis is that things are different from each other, or different from a theoretical expectation.

For example, one alternative hypothesis would be that male chickens have a different average foot size than female chickens; another would be that the sex ratio is different from $1:1$.

Usually, the null hypothesis is boring and the alternative hypothesis is interesting. For example, let's say you feed chocolate to a bunch of chickens, then look at the sex ratio in their offspring. If you get more females than males, it would be a tremendously exciting discovery: it would be a fundamental discovery about the mechanism of sex determination, female chickens are more valuable than male chickens in egg-laying breeds, and you'd be able to publish your result in Science or Nature . Lots of people have spent a lot of time and money trying to change the sex ratio in chickens, and if you're successful, you'll be rich and famous. But if the chocolate doesn't change the sex ratio, it would be an extremely boring result, and you'd have a hard time getting it published in the Eastern Delaware Journal of Chickenology . It's therefore tempting to look for patterns in your data that support the exciting alternative hypothesis. For example, you might look at $48$ offspring of chocolate-fed chickens and see $31$ females and only $17$ males. This looks promising, but before you get all happy and start buying formal wear for the Nobel Prize ceremony, you need to ask "What's the probability of getting a deviation from the null expectation that large, just by chance, if the boring null hypothesis is really true?" Only when that probability is low can you reject the null hypothesis. The goal of statistical hypothesis testing is to estimate the probability of getting your observed results under the null hypothesis.

Biological vs. Statistical Null Hypotheses

It is important to distinguish between biological null and alternative hypotheses and statistical null and alternative hypotheses. "Sexual selection by females has caused male chickens to evolve bigger feet than females" is a biological alternative hypothesis; it says something about biological processes, in this case sexual selection. "Male chickens have a different average foot size than females" is a statistical alternative hypothesis; it says something about the numbers, but nothing about what caused those numbers to be different. The biological null and alternative hypotheses are the first that you should think of, as they describe something interesting about biology; they are two possible answers to the biological question you are interested in ("What affects foot size in chickens?"). The statistical null and alternative hypotheses are statements about the data that should follow from the biological hypotheses: if sexual selection favors bigger feet in male chickens (a biological hypothesis), then the average foot size in male chickens should be larger than the average in females (a statistical hypothesis). If you reject the statistical null hypothesis, you then have to decide whether that's enough evidence that you can reject your biological null hypothesis. For example, if you don't find a significant difference in foot size between male and female chickens, you could conclude "There is no significant evidence that sexual selection has caused male chickens to have bigger feet." If you do find a statistically significant difference in foot size, that might not be enough for you to conclude that sexual selection caused the bigger feet; it might be that males eat more, or that the bigger feet are a developmental byproduct of the roosters' combs, or that males run around more and the exercise makes their feet bigger. When there are multiple biological interpretations of a statistical result, you need to think of additional experiments to test the different possibilities.

Testing the Null Hypothesis

The primary goal of a statistical test is to determine whether an observed data set is so different from what you would expect under the null hypothesis that you should reject the null hypothesis. For example, let's say you are studying sex determination in chickens. For breeds of chickens that are bred to lay lots of eggs, female chicks are more valuable than male chicks, so if you could figure out a way to manipulate the sex ratio, you could make a lot of chicken farmers very happy. You've fed chocolate to a bunch of female chickens (in birds, unlike mammals, the female parent determines the sex of the offspring), and you get $25$ female chicks and $23$ male chicks. Anyone would look at those numbers and see that they could easily result from chance; there would be no reason to reject the null hypothesis of a $1:1$ ratio of females to males. If you got $47$ females and $1$ male, most people would look at those numbers and see that they would be extremely unlikely to happen due to luck, if the null hypothesis were true; you would reject the null hypothesis and conclude that chocolate really changed the sex ratio. However, what if you had $31$ females and $17$ males? That's definitely more females than males, but is it really so unlikely to occur due to chance that you can reject the null hypothesis? To answer that, you need more than common sense, you need to calculate the probability of getting a deviation that large due to chance.

In the figure above, I used the BINOMDIST function of Excel to calculate the probability of getting each possible number of males, from $0$ to $48$, under the null hypothesis that $0.5$ are male. As you can see, the probability of getting $17$ males out of $48$ total chickens is about $0.015$. That seems like a pretty small probability, doesn't it? However, that's the probability of getting exactly $17$ males. What you want to know is the probability of getting $17$ or fewer males. If you were going to accept $17$ males as evidence that the sex ratio was biased, you would also have accepted $16$, or $15$, or $14$,… males as evidence for a biased sex ratio. You therefore need to add together the probabilities of all these outcomes. The probability of getting $17$ or fewer males out of $48$, under the null hypothesis, is $0.030$. That means that if you had an infinite number of chickens, half males and half females, and you took a bunch of random samples of $48$ chickens, $3.0\%$ of the samples would have $17$ or fewer males.

This number, $0.030$, is the $P$ value. It is defined as the probability of getting the observed result, or a more extreme result, if the null hypothesis is true. So "$P=0.030$" is a shorthand way of saying "The probability of getting $17$ or fewer male chickens out of $48$ total chickens, IF the null hypothesis is true that $50\%$ of chickens are male, is $0.030$."

False Positives vs. False Negatives

After you do a statistical test, you are either going to reject or accept the null hypothesis. Rejecting the null hypothesis means that you conclude that the null hypothesis is not true; in our chicken sex example, you would conclude that the true proportion of male chicks, if you gave chocolate to an infinite number of chicken mothers, would be less than $50\%$.

When you reject a null hypothesis, there's a chance that you're making a mistake. The null hypothesis might really be true, and it may be that your experimental results deviate from the null hypothesis purely as a result of chance. In a sample of $48$ chickens, it's possible to get $17$ male chickens purely by chance; it's even possible (although extremely unlikely) to get $0$ male and $48$ female chickens purely by chance, even though the true proportion is $50\%$ males. This is why we never say we "prove" something in science; there's always a chance, however miniscule, that our data are fooling us and deviate from the null hypothesis purely due to chance. When your data fool you into rejecting the null hypothesis even though it's true, it's called a "false positive," or a "Type I error." So another way of defining the $P$ value is the probability of getting a false positive like the one you've observed, if the null hypothesis is true.

Another way your data can fool you is when you don't reject the null hypothesis, even though it's not true. If the true proportion of female chicks is $51\%$, the null hypothesis of a $50\%$ proportion is not true, but you're unlikely to get a significant difference from the null hypothesis unless you have a huge sample size. Failing to reject the null hypothesis, even though it's not true, is a "false negative" or "Type II error." This is why we never say that our data shows the null hypothesis to be true; all we can say is that we haven't rejected the null hypothesis.

Significance Levels

Does a probability of $0.030$ mean that you should reject the null hypothesis, and conclude that chocolate really caused a change in the sex ratio? The convention in most biological research is to use a significance level of $0.05$. This means that if the $P$ value is less than $0.05$, you reject the null hypothesis; if $P$ is greater than or equal to $0.05$, you don't reject the null hypothesis. There is nothing mathematically magic about $0.05$, it was chosen rather arbitrarily during the early days of statistics; people could have agreed upon $0.04$, or $0.025$, or $0.071$ as the conventional significance level.

The significance level (also known as the "critical value" or "alpha") you should use depends on the costs of different kinds of errors. With a significance level of $0.05$, you have a $5\%$ chance of rejecting the null hypothesis, even if it is true. If you try $100$ different treatments on your chickens, and none of them really change the sex ratio, $5\%$ of your experiments will give you data that are significantly different from a $1:1$ sex ratio, just by chance. In other words, $5\%$ of your experiments will give you a false positive. If you use a higher significance level than the conventional $0.05$, such as $0.10$, you will increase your chance of a false positive to $0.10$ (therefore increasing your chance of an embarrassingly wrong conclusion), but you will also decrease your chance of a false negative (increasing your chance of detecting a subtle effect). If you use a lower significance level than the conventional $0.05$, such as $0.01$, you decrease your chance of an embarrassing false positive, but you also make it less likely that you'll detect a real deviation from the null hypothesis if there is one.

The relative costs of false positives and false negatives, and thus the best $P$ value to use, will be different for different experiments. If you are screening a bunch of potential sex-ratio-changing treatments and get a false positive, it wouldn't be a big deal; you'd just run a few more tests on that treatment until you were convinced the initial result was a false positive. The cost of a false negative, however, would be that you would miss out on a tremendously valuable discovery. You might therefore set your significance value to $0.10$ or more for your initial tests. On the other hand, once your sex-ratio-changing treatment is undergoing final trials before being sold to farmers, a false positive could be very expensive; you'd want to be very confident that it really worked. Otherwise, if you sell the chicken farmers a sex-ratio treatment that turns out to not really work (it was a false positive), they'll sue the pants off of you. Therefore, you might want to set your significance level to $0.01$, or even lower, for your final tests.

The significance level you choose should also depend on how likely you think it is that your alternative hypothesis will be true, a prediction that you make before you do the experiment. This is the foundation of Bayesian statistics, as explained below.

You must choose your significance level before you collect the data, of course. If you choose to use a different significance level than the conventional $0.05$, people will be skeptical; you must be able to justify your choice. Throughout this handbook, I will always use $P< 0.05$ as the significance level. If you are doing an experiment where the cost of a false positive is a lot greater or smaller than the cost of a false negative, or an experiment where you think it is unlikely that the alternative hypothesis will be true, you should consider using a different significance level.

One-tailed vs. Two-tailed Probabilities

The probability that was calculated above, $0.030$, is the probability of getting $17$ or fewer males out of $48$. It would be significant, using the conventional $P< 0.05$criterion. However, what about the probability of getting $17$ or fewer females? If your null hypothesis is "The proportion of males is $17$ or more" and your alternative hypothesis is "The proportion of males is less than $0.5$," then you would use the $P=0.03$ value found by adding the probabilities of getting $17$ or fewer males. This is called a one-tailed probability, because you are adding the probabilities in only one tail of the distribution shown in the figure. However, if your null hypothesis is "The proportion of males is $0.5$", then your alternative hypothesis is "The proportion of males is different from $0.5$." In that case, you should add the probability of getting $17$ or fewer females to the probability of getting $17$ or fewer males. This is called a two-tailed probability. If you do that with the chicken result, you get $P=0.06$, which is not quite significant.

You should decide whether to use the one-tailed or two-tailed probability before you collect your data, of course. A one-tailed probability is more powerful, in the sense of having a lower chance of false negatives, but you should only use a one-tailed probability if you really, truly have a firm prediction about which direction of deviation you would consider interesting. In the chicken example, you might be tempted to use a one-tailed probability, because you're only looking for treatments that decrease the proportion of worthless male chickens. But if you accidentally found a treatment that produced $87\%$ male chickens, would you really publish the result as "The treatment did not cause a significant decrease in the proportion of male chickens"? I hope not. You'd realize that this unexpected result, even though it wasn't what you and your farmer friends wanted, would be very interesting to other people; by leading to discoveries about the fundamental biology of sex-determination in chickens, in might even help you produce more female chickens someday. Any time a deviation in either direction would be interesting, you should use the two-tailed probability. In addition, people are skeptical of one-tailed probabilities, especially if a one-tailed probability is significant and a two-tailed probability would not be significant (as in our chocolate-eating chicken example). Unless you provide a very convincing explanation, people may think you decided to use the one-tailed probability after you saw that the two-tailed probability wasn't quite significant, which would be cheating. It may be easier to always use two-tailed probabilities. For this handbook, I will always use two-tailed probabilities, unless I make it very clear that only one direction of deviation from the null hypothesis would be interesting.

Reporting your results

In the olden days, when people looked up $P$ values in printed tables, they would report the results of a statistical test as "$P< 0.05$", "$P< 0.01$", "$P>0.10$", etc. Nowadays, almost all computer statistics programs give the exact $P$ value resulting from a statistical test, such as $P=0.029$, and that's what you should report in your publications. You will conclude that the results are either significant or they're not significant; they either reject the null hypothesis (if $P$ is below your pre-determined significance level) or don't reject the null hypothesis (if $P$ is above your significance level). But other people will want to know if your results are "strongly" significant ($P$ much less than $0.05$), which will give them more confidence in your results than if they were "barely" significant ($P=0.043$, for example). In addition, other researchers will need the exact $P$ value if they want to combine your results with others into a meta-analysis.

Computer statistics programs can give somewhat inaccurate $P$ values when they are very small. Once your $P$ values get very small, you can just say "$P< 0.00001$" or some other impressively small number. You should also give either your raw data, or the test statistic and degrees of freedom, in case anyone wants to calculate your exact $P$ value.

Effect Sizes and Confidence Intervals

A fairly common criticism of the hypothesis-testing approach to statistics is that the null hypothesis will always be false, if you have a big enough sample size. In the chicken-feet example, critics would argue that if you had an infinite sample size, it is impossible that male chickens would have exactly the same average foot size as female chickens. Therefore, since you know before doing the experiment that the null hypothesis is false, there's no point in testing it.

This criticism only applies to two-tailed tests, where the null hypothesis is "Things are exactly the same" and the alternative is "Things are different." Presumably these critics think it would be okay to do a one-tailed test with a null hypothesis like "Foot length of male chickens is the same as, or less than, that of females," because the null hypothesis that male chickens have smaller feet than females could be true. So if you're worried about this issue, you could think of a two-tailed test, where the null hypothesis is that things are the same, as shorthand for doing two one-tailed tests. A significant rejection of the null hypothesis in a two-tailed test would then be the equivalent of rejecting one of the two one-tailed null hypotheses.

A related criticism is that a significant rejection of a null hypothesis might not be biologically meaningful, if the difference is too small to matter. For example, in the chicken-sex experiment, having a treatment that produced $49.9\%$ male chicks might be significantly different from $50\%$, but it wouldn't be enough to make farmers want to buy your treatment. These critics say you should estimate the effect size and put a confidence interval on it, not estimate a $P$ value. So the goal of your chicken-sex experiment should not be to say "Chocolate gives a proportion of males that is significantly less than $50\%$ (($P=0.015$)" but to say "Chocolate produced $36.1\%$ males with a $95\%$ confidence interval of $25.9\%$ to $47.4\%$." For the chicken-feet experiment, you would say something like "The difference between males and females in mean foot size is $2.45mm$, with a confidence interval on the difference of $\pm 1.98mm$."

Estimating effect sizes and confidence intervals is a useful way to summarize your results, and it should usually be part of your data analysis; you'll often want to include confidence intervals in a graph. However, there are a lot of experiments where the goal is to decide a yes/no question, not estimate a number. In the initial tests of chocolate on chicken sex ratio, the goal would be to decide between "It changed the sex ratio" and "It didn't seem to change the sex ratio." Any change in sex ratio that is large enough that you could detect it would be interesting and worth follow-up experiments. While it's true that the difference between $49.9\%$ and $50\%$ might not be worth pursuing, you wouldn't do an experiment on enough chickens to detect a difference that small.

Often, the people who claim to avoid hypothesis testing will say something like "the $95\%$ confidence interval of $25.9\%$ to $47.4\%$ does not include $50\%$, so we conclude that the plant extract significantly changed the sex ratio." This is a clumsy and roundabout form of hypothesis testing, and they might as well admit it and report the $P$ value.

Bayesian statistics

Another alternative to frequentist statistics is Bayesian statistics. A key difference is that Bayesian statistics requires specifying your best guess of the probability of each possible value of the parameter to be estimated, before the experiment is done. This is known as the "prior probability." So for your chicken-sex experiment, you're trying to estimate the "true" proportion of male chickens that would be born, if you had an infinite number of chickens. You would have to specify how likely you thought it was that the true proportion of male chickens was $50\%$, or $51\%$, or $52\%$, or $47.3\%$, etc. You would then look at the results of your experiment and use the information to calculate new probabilities that the true proportion of male chickens was $50\%$, or $51\%$, or $52\%$, or $47.3\%$, etc. (the posterior distribution).

I'll confess that I don't really understand Bayesian statistics, and I apologize for not explaining it well. In particular, I don't understand how people are supposed to come up with a prior distribution for the kinds of experiments that most biologists do. With the exception of systematics, where Bayesian estimation of phylogenies is quite popular and seems to make sense, I haven't seen many research biologists using Bayesian statistics for routine data analysis of simple laboratory experiments. This means that even if the cult-like adherents of Bayesian statistics convinced you that they were right, you would have a difficult time explaining your results to your biologist peers. Statistics is a method of conveying information, and if you're speaking a different language than the people you're talking to, you won't convey much information. So I'll stick with traditional frequentist statistics for this handbook.

Having said that, there's one key concept from Bayesian statistics that is important for all users of statistics to understand. To illustrate it, imagine that you are testing extracts from $1000$ different tropical plants, trying to find something that will kill beetle larvae. The reality (which you don't know) is that $500$ of the extracts kill beetle larvae, and $500$ don't. You do the $1000$ experiments and do the $1000$ frequentist statistical tests, and you use the traditional significance level of $P< 0.05$. The $500$ plant extracts that really work all give you $P< 0.05$; these are the true positives. Of the $500$ extracts that don't work, $5\%$ of them give you $P< 0.05$ by chance (this is the meaning of the $P$ value, after all), so you have $25$ false positives. So you end up with $525$ plant extracts that gave you a $P$ value less than $0.05$. You'll have to do further experiments to figure out which are the $25$ false positives and which are the $500$ true positives, but that's not so bad, since you know that most of them will turn out to be true positives.

Now imagine that you are testing those extracts from $1000$ different tropical plants to try to find one that will make hair grow. The reality (which you don't know) is that one of the extracts makes hair grow, and the other $999$ don't. You do the $1000$ experiments and do the $1000$ frequentist statistical tests, and you use the traditional significance level of $P< 0.05$. The one plant extract that really works gives you P <0.05; this is the true positive. But of the $999$ extracts that don't work, $5\%$ of them give you $P< 0.05$ by chance, so you have about $50$ false positives. You end up with $51$ $P$ values less than $0.05$, but almost all of them are false positives.

Now instead of testing $1000$ plant extracts, imagine that you are testing just one. If you are testing it to see if it kills beetle larvae, you know (based on everything you know about plant and beetle biology) there's a pretty good chance it will work, so you can be pretty sure that a $P$ value less than $0.05$ is a true positive. But if you are testing that one plant extract to see if it grows hair, which you know is very unlikely (based on everything you know about plants and hair), a $P$ value less than $0.05$ is almost certainly a false positive. In other words, if you expect that the null hypothesis is probably true, a statistically significant result is probably a false positive. This is sad; the most exciting, amazing, unexpected results in your experiments are probably just your data trying to make you jump to ridiculous conclusions. You should require a much lower $P$ value to reject a null hypothesis that you think is probably true.

A Bayesian would insist that you put in numbers just how likely you think the null hypothesis and various values of the alternative hypothesis are, before you do the experiment, and I'm not sure how that is supposed to work in practice for most experimental biology. But the general concept is a valuable one: as Carl Sagan summarized it, "Extraordinary claims require extraordinary evidence."

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Here are three experiments to illustrate when the different approaches to statistics are appropriate. In the first experiment, you are testing a plant extract on rabbits to see if it will lower their blood pressure. You already know that the plant extract is a diuretic (makes the rabbits pee more) and you already know that diuretics tend to lower blood pressure, so you think there's a good chance it will work. If it does work, you'll do more low-cost animal tests on it before you do expensive, potentially risky human trials. Your prior expectation is that the null hypothesis (that the plant extract has no effect) has a good chance of being false, and the cost of a false positive is fairly low. So you should do frequentist hypothesis testing, with a significance level of $0.05$.

In the second experiment, you are going to put human volunteers with high blood pressure on a strict low-salt diet and see how much their blood pressure goes down. Everyone will be confined to a hospital for a month and fed either a normal diet, or the same foods with half as much salt. For this experiment, you wouldn't be very interested in the $P$ value, as based on prior research in animals and humans, you are already quite certain that reducing salt intake will lower blood pressure; you're pretty sure that the null hypothesis that "Salt intake has no effect on blood pressure" is false. Instead, you are very interested to know how much the blood pressure goes down. Reducing salt intake in half is a big deal, and if it only reduces blood pressure by $1mm$ Hg, the tiny gain in life expectancy wouldn't be worth a lifetime of bland food and obsessive label-reading. If it reduces blood pressure by $20mm$ with a confidence interval of $\pm 5mm$, it might be worth it. So you should estimate the effect size (the difference in blood pressure between the diets) and the confidence interval on the difference.

In the third experiment, you are going to put magnetic hats on guinea pigs and see if their blood pressure goes down (relative to guinea pigs wearing the kind of non-magnetic hats that guinea pigs usually wear). This is a really goofy experiment, and you know that it is very unlikely that the magnets will have any effect (it's not impossible—magnets affect the sense of direction of homing pigeons, and maybe guinea pigs have something similar in their brains and maybe it will somehow affect their blood pressure—it just seems really unlikely). You might analyze your results using Bayesian statistics, which will require specifying in numerical terms just how unlikely you think it is that the magnetic hats will work. Or you might use frequentist statistics, but require a $P$ value much, much lower than $0.05$ to convince yourself that the effect is real.

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Hypothesis Testing

What is Hypothesis Testing?

Hypothesis testing in statistics refers to analyzing an assumption about a population parameter. It is used to make an educated guess about an assumption using statistics. With the use of sample data, hypothesis testing makes an assumption about how true the assumption is for the entire population from where the sample is being taken.

Any hypothetical statement we make may or may not be valid, and it is then our responsibility to provide evidence for its possibility. To approach any hypothesis, we follow these four simple steps that test its validity.

First, we formulate two hypothetical statements such that only one of them is true. By doing so, we can check the validity of our own hypothesis.

The next step is to formulate the statistical analysis to be followed based upon the data points.

Then we analyze the given data using our methodology.

The final step is to analyze the result and judge whether the null hypothesis will be rejected or is true.

Let’s look at several hypothesis testing examples:

It is observed that the average recovery time for a knee-surgery patient is 8 weeks. A physician believes that after successful knee surgery if the patient goes for physical therapy twice a week rather than thrice a week, the recovery period will be longer. Conduct hypothesis for this statement.

David is a ten-year-old who finishes a 25-yard freestyle in the meantime of 16.43 seconds. David’s father bought goggles for his son, believing that it would help him to reduce his time. He then recorded a total of fifteen 25-yard freestyle for David, and the average time came out to be 16 seconds. Conduct a hypothesis.

A tire company claims their A-segment of tires have a running life of 50,000 miles before they need to be replaced, and previous studies show a standard deviation of 8,000 miles. After surveying a total of 28 tires, the mean run time came to be 46,500 miles with a standard deviation of 9800 miles. Is the claim made by the tire company consistent with the given data? Conduct hypothesis testing.

All of the hypothesis testing examples are from real-life situations, which leads us to believe that hypothesis testing is a very practical topic indeed. It is an integral part of a researcher's study and is used in every research methodology in one way or another.

Inferential statistics majorly deals with hypothesis testing. The research hypothesis states there is a relationship between the independent variable and dependent variable. Whereas the null hypothesis rejects this claim of any relationship between the two, our job as researchers or students is to check whether there is any relation between the two.

Hypothesis Testing in Research Methodology

Now that we are clear about what hypothesis testing is? Let's look at the use of hypothesis testing in research methodology. Hypothesis testing is at the centre of research projects.

What is Hypothesis Testing and Why is it Important in Research Methodology?

Often after formulating research statements, the validity of those statements need to be verified. Hypothesis testing offers a statistical approach to the researcher about the theoretical assumptions he/she made. It can be understood as quantitative results for a qualitative problem.

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Hypothesis testing provides various techniques to test the hypothesis statement depending upon the variable and the data points. It finds its use in almost every field of research while answering statements such as whether this new medicine will work, a new testing method is appropriate, or if the outcomes of a random experiment are probable or not.

Procedure of Hypothesis Testing

To find the validity of any statement, we have to strictly follow the stepwise procedure of hypothesis testing. After stating the initial hypothesis, we have to re-write them in the form of a null and alternate hypothesis. The alternate hypothesis predicts a relationship between the variables, whereas the null hypothesis predicts no relationship between the variables.

After writing them as H 0 (null hypothesis) and H a (Alternate hypothesis), only one of the statements can be true. For example, taking the hypothesis that, on average, men are taller than women, we write the statements as:

H 0 : On average, men are not taller than women.

H a : On average, men are taller than women.

Our next aim is to collect sample data, what we call sampling, in a way so that we can test our hypothesis. Your data should come from the concerned population for which you want to make a hypothesis.

What is the p value in hypothesis testing? P-value gives us information about the probability of occurrence of results as extreme as observed results.

You will obtain your p-value after choosing the hypothesis testing method, which will be the guiding factor in rejecting the hypothesis. Usually, the p-value cutoff for rejecting the null hypothesis is 0.05. So anything below that, you will reject the null hypothesis.

A low p-value means that the between-group variance is large enough that there is almost no overlapping, and it is unlikely that these came about by chance. A high p-value suggests there is a high within-group variance and low between-group variance, and any difference in the measure is due to chance only.

What is statistical hypothesis testing?

When forming conclusions through research, two sorts of errors are common: A hypothesis must be set and defined in statistics during a statistical survey or research. A statistical hypothesis is what it is called. It is, in fact, a population parameter assumption. However, it is unmistakable that this idea is always proven correct. Hypothesis testing refers to the predetermined formal procedures used by statisticians to determine whether hypotheses should be accepted or rejected. The process of selecting hypotheses for a given probability distribution based on observable data is known as hypothesis testing. Hypothesis testing is a fundamental and crucial issue in statistics.

Why do I Need to Test it? Why not just prove an alternate one?

The quick answer is that you must as a scientist; it is part of the scientific process. Science employs a variety of methods to test or reject theories, ensuring that any new hypothesis is free of errors. One protection to ensure your research is not incorrect is to include both a null and an alternate hypothesis. The scientific community considers not incorporating the null hypothesis in your research to be poor practice. You are almost certainly setting yourself up for failure if you set out to prove another theory without first examining it. At the very least, your experiment will not be considered seriously.

Types of Hypothesis Testing

There are several types of hypothesis testing, and they are used based on the data provided. Depending on the sample size and the data given, we choose among different hypothesis testing methodologies. Here starts the use of hypothesis testing tools in research methodology.

Normality- This type of testing is used for normal distribution in a population sample. If the data points are grouped around the mean, the probability of them being above or below the mean is equally likely. Its shape resembles a bell curve that is equally distributed on either side of the mean.

T-test- This test is used when the sample size in a normally distributed population is comparatively small, and the standard deviation is unknown. Usually, if the sample size drops below 30, we use a T-test to find the confidence intervals of the population.

Chi-Square Test- The Chi-Square test is used to test the population variance against the known or assumed value of the population variance. It is also a better choice to test the goodness of fit of a distribution of data. The two most common Chi-Square tests are the Chi-Square test of independence and the chi-square test of variance.

ANOVA- Analysis of Variance or ANOVA compares the data sets of two different populations or samples. It is similar in its use to the t-test or the Z-test, but it allows us to compare more than two sample means. ANOVA allows us to test the significance between an independent variable and a dependent variable, namely X and Y, respectively.

Z-test- It is a statistical measure to test that the means of two population samples are different when their variance is known. For a Z-test, the population is assumed to be normally distributed. A z-test is better suited in the case of large sample sizes greater than 30. This is due to the central limit theorem that as the sample size increases, the samples are considered to be distributed normally.

FAQs on Hypothesis Testing

1. Mention the types of hypothesis Tests.

There are two types of a hypothesis tests:

Null Hypothesis: It is denoted as H₀.

Alternative Hypothesis: IT is denoted as H₁ or Hₐ.

2. What are the two errors that can be found while performing the null Hypothesis test?

While performing the null hypothesis test there is a possibility of occurring two types of errors,

Type-1: The type-1 error is denoted by (α), it is also known as the significance level. It is the rejection of the true null hypothesis. It is the error of commission.

Type-2: The type-2 error is denoted by (β). (1 - β) is known as the power test. The false null hypothesis is not rejected. It is the error of the omission.

3. What is the p-value in hypothesis testing?

During hypothetical testing in statistics, the p-value indicates the probability of obtaining the result as extreme as observed results. A smaller p-value provides evidence to accept the alternate hypothesis. The p-value is used as a rejection point that provides the smallest level of significance at which the null hypothesis is rejected. Often p-value is calculated using the p-value tables by calculating the deviation between the observed value and the chosen reference value.

It may also be calculated mathematically by performing integrals on all the values that fall under the curve and areas far from the reference value as the observed value relative to the total area of the curve. The p-value determines the evidence to reject the null hypothesis in hypothesis testing.

4. What is a null hypothesis?

The null hypothesis in statistics says that there is no certain difference between the population. It serves as a conjecture proposing no difference, whereas the alternate hypothesis says there is a difference. When we perform hypothesis testing, we have to state the null hypothesis and alternative hypotheses such that only one of them is ever true.

By determining the p-value, we calculate whether the null hypothesis is to be rejected or not. If the difference between groups is low, it is merely by chance, and the null hypothesis, which states that there is no difference among groups, is true. Therefore, we have no evidence to reject the null hypothesis.

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Hypothesis testing involves formulating assumptions about population parameters based on sample statistics and rigorously evaluating these assumptions against empirical evidence. This article sheds light on the significance of hypothesis testing and the critical steps involved in the process.

What is Hypothesis Testing?

Hypothesis testing is a statistical method that is used to make a statistical decision using experimental data. Hypothesis testing is basically an assumption that we make about a population parameter. It evaluates two mutually exclusive statements about a population to determine which statement is best supported by the sample data.

Example: You say an average height in the class is 30 or a boy is taller than a girl. All of these is an assumption that we are assuming, and we need some statistical way to prove these. We need some mathematical conclusion whatever we are assuming is true.

Defining Hypotheses

$\mu$

Key Terms of Hypothesis Testing

$\alpha$

P-value: The P value , or calculated probability, is the probability of finding the observed/extreme results when the null hypothesis(H0) of a study-given problem is true. If your P-value is less than the chosen significance level then you reject the null hypothesis i.e. accept that your sample claims to support the alternative hypothesis.
Test Statistic: The test statistic is a numerical value calculated from sample data during a hypothesis test, used to determine whether to reject the null hypothesis. It is compared to a critical value or p-value to make decisions about the statistical significance of the observed results.
Critical value : The critical value in statistics is a threshold or cutoff point used to determine whether to reject the null hypothesis in a hypothesis test.
Degrees of freedom: Degrees of freedom are associated with the variability or freedom one has in estimating a parameter. The degrees of freedom are related to the sample size and determine the shape.

Why do we use Hypothesis Testing?

Hypothesis testing is an important procedure in statistics. Hypothesis testing evaluates two mutually exclusive population statements to determine which statement is most supported by sample data. When we say that the findings are statistically significant, thanks to hypothesis testing.

One-Tailed and Two-Tailed Test

One tailed test focuses on one direction, either greater than or less than a specified value. We use a one-tailed test when there is a clear directional expectation based on prior knowledge or theory. The critical region is located on only one side of the distribution curve. If the sample falls into this critical region, the null hypothesis is rejected in favor of the alternative hypothesis.

One-Tailed Test

There are two types of one-tailed test:

$\mu \geq 50$

Two-Tailed Test

A two-tailed test considers both directions, greater than and less than a specified value.We use a two-tailed test when there is no specific directional expectation, and want to detect any significant difference.

$\mu =$

What are Type 1 and Type 2 errors in Hypothesis Testing?

In hypothesis testing, Type I and Type II errors are two possible errors that researchers can make when drawing conclusions about a population based on a sample of data. These errors are associated with the decisions made regarding the null hypothesis and the alternative hypothesis.

$\alpha$

How does Hypothesis Testing work?

Step 1: define null and alternative hypothesis.

We first identify the problem about which we want to make an assumption keeping in mind that our assumption should be contradictory to one another, assuming Normally distributed data.

Step 2 – Choose significance level

$\alpha$

Step 3 – Collect and Analyze data.

Gather relevant data through observation or experimentation. Analyze the data using appropriate statistical methods to obtain a test statistic.

Step 4-Calculate Test Statistic

The data for the tests are evaluated in this step we look for various scores based on the characteristics of data. The choice of the test statistic depends on the type of hypothesis test being conducted.

There are various hypothesis tests, each appropriate for various goal to calculate our test. This could be a Z-test , Chi-square , T-test , and so on.

Z-test : If population means and standard deviations are known. Z-statistic is commonly used.
t-test : If population standard deviations are unknown. and sample size is small than t-test statistic is more appropriate.
Chi-square test : Chi-square test is used for categorical data or for testing independence in contingency tables
F-test : F-test is often used in analysis of variance (ANOVA) to compare variances or test the equality of means across multiple groups.

We have a smaller dataset, So, T-test is more appropriate to test our hypothesis.

T-statistic is a measure of the difference between the means of two groups relative to the variability within each group. It is calculated as the difference between the sample means divided by the standard error of the difference. It is also known as the t-value or t-score.

Step 5 – Comparing Test Statistic:

In this stage, we decide where we should accept the null hypothesis or reject the null hypothesis. There are two ways to decide where we should accept or reject the null hypothesis.

Method A: Using Crtical values

Comparing the test statistic and tabulated critical value we have,

If Test Statistic>Critical Value: Reject the null hypothesis.
If Test Statistic≤Critical Value: Fail to reject the null hypothesis.

Note: Critical values are predetermined threshold values that are used to make a decision in hypothesis testing. To determine critical values for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Method B: Using P-values

We can also come to an conclusion using the p-value,

$p\leq\alpha$

Note : The p-value is the probability of obtaining a test statistic as extreme as, or more extreme than, the one observed in the sample, assuming the null hypothesis is true. To determine p-value for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Step 7- Interpret the Results

At last, we can conclude our experiment using method A or B.

Calculating test statistic

To validate our hypothesis about a population parameter we use statistical functions . We use the z-score, p-value, and level of significance(alpha) to make evidence for our hypothesis for normally distributed data .

1. Z-statistics:

When population means and standard deviations are known.

$z = \frac{\bar{x} - \mu}{\frac{\sigma}{\sqrt{n}}}$

μ represents the population mean,
σ is the standard deviation
and n is the size of the sample.

2. T-Statistics

T test is used when n<30,

t-statistic calculation is given by:

$t=\frac{x̄-μ}{s/\sqrt{n}}$

t = t-score,
x̄ = sample mean
μ = population mean,
s = standard deviation of the sample,
n = sample size

3. Chi-Square Test

Chi-Square Test for Independence categorical Data (Non-normally distributed) using:

$\chi^2 = \sum \frac{(O_{ij} - E_{ij})^2}{E_{ij}}$

i,j are the rows and columns index respectively.

$E_{ij}$

Real life Hypothesis Testing example

Let’s examine hypothesis testing using two real life situations,

Case A: D oes a New Drug Affect Blood Pressure?

Imagine a pharmaceutical company has developed a new drug that they believe can effectively lower blood pressure in patients with hypertension. Before bringing the drug to market, they need to conduct a study to assess its impact on blood pressure.

Before Treatment: 120, 122, 118, 130, 125, 128, 115, 121, 123, 119
After Treatment: 115, 120, 112, 128, 122, 125, 110, 117, 119, 114

Step 1 : Define the Hypothesis

Null Hypothesis : (H 0 )The new drug has no effect on blood pressure.
Alternate Hypothesis : (H 1 )The new drug has an effect on blood pressure.

Step 2: Define the Significance level

Let’s consider the Significance level at 0.05, indicating rejection of the null hypothesis.

If the evidence suggests less than a 5% chance of observing the results due to random variation.

Step 3 : Compute the test statistic

Using paired T-test analyze the data to obtain a test statistic and a p-value.

The test statistic (e.g., T-statistic) is calculated based on the differences between blood pressure measurements before and after treatment.

t = m/(s/√n)

m = mean of the difference i.e X after, X before
s = standard deviation of the difference (d) i.e d i = X after, i − X before,
n = sample size,

then, m= -3.9, s= 1.8 and n= 10

we, calculate the , T-statistic = -9 based on the formula for paired t test

Step 4: Find the p-value

The calculated t-statistic is -9 and degrees of freedom df = 9, you can find the p-value using statistical software or a t-distribution table.

thus, p-value = 8.538051223166285e-06

Step 5: Result

If the p-value is less than or equal to 0.05, the researchers reject the null hypothesis.
If the p-value is greater than 0.05, they fail to reject the null hypothesis.

Conclusion: Since the p-value (8.538051223166285e-06) is less than the significance level (0.05), the researchers reject the null hypothesis. There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different.

Python Implementation of Hypothesis Testing

Let’s create hypothesis testing with python, where we are testing whether a new drug affects blood pressure. For this example, we will use a paired T-test. We’ll use the scipy.stats library for the T-test.

Scipy is a mathematical library in Python that is mostly used for mathematical equations and computations.

We will implement our first real life problem via python,

In the above example, given the T-statistic of approximately -9 and an extremely small p-value, the results indicate a strong case to reject the null hypothesis at a significance level of 0.05.

The results suggest that the new drug, treatment, or intervention has a significant effect on lowering blood pressure.
The negative T-statistic indicates that the mean blood pressure after treatment is significantly lower than the assumed population mean before treatment.

Case B : Cholesterol level in a population

Data: A sample of 25 individuals is taken, and their cholesterol levels are measured.

Cholesterol Levels (mg/dL): 205, 198, 210, 190, 215, 205, 200, 192, 198, 205, 198, 202, 208, 200, 205, 198, 205, 210, 192, 205, 198, 205, 210, 192, 205.

Populations Mean = 200

Population Standard Deviation (σ): 5 mg/dL(given for this problem)

Step 1: Define the Hypothesis

Null Hypothesis (H 0 ): The average cholesterol level in a population is 200 mg/dL.
Alternate Hypothesis (H 1 ): The average cholesterol level in a population is different from 200 mg/dL.

As the direction of deviation is not given , we assume a two-tailed test, and based on a normal distribution table, the critical values for a significance level of 0.05 (two-tailed) can be calculated through the z-table and are approximately -1.96 and 1.96.

$(203.8 - 200) / (5 \div \sqrt{25})$

Step 4: Result

Since the absolute value of the test statistic (2.04) is greater than the critical value (1.96), we reject the null hypothesis. And conclude that, there is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL

Limitations of Hypothesis Testing

Although a useful technique, hypothesis testing does not offer a comprehensive grasp of the topic being studied. Without fully reflecting the intricacy or whole context of the phenomena, it concentrates on certain hypotheses and statistical significance.
The accuracy of hypothesis testing results is contingent on the quality of available data and the appropriateness of statistical methods used. Inaccurate data or poorly formulated hypotheses can lead to incorrect conclusions.
Relying solely on hypothesis testing may cause analysts to overlook significant patterns or relationships in the data that are not captured by the specific hypotheses being tested. This limitation underscores the importance of complimenting hypothesis testing with other analytical approaches.

Hypothesis testing stands as a cornerstone in statistical analysis, enabling data scientists to navigate uncertainties and draw credible inferences from sample data. By systematically defining null and alternative hypotheses, choosing significance levels, and leveraging statistical tests, researchers can assess the validity of their assumptions. The article also elucidates the critical distinction between Type I and Type II errors, providing a comprehensive understanding of the nuanced decision-making process inherent in hypothesis testing. The real-life example of testing a new drug’s effect on blood pressure using a paired T-test showcases the practical application of these principles, underscoring the importance of statistical rigor in data-driven decision-making.

Frequently Asked Questions (FAQs)

1. what are the 3 types of hypothesis test.

There are three types of hypothesis tests: right-tailed, left-tailed, and two-tailed. Right-tailed tests assess if a parameter is greater, left-tailed if lesser. Two-tailed tests check for non-directional differences, greater or lesser.

2.What are the 4 components of hypothesis testing?

Null Hypothesis ( ): No effect or difference exists. Alternative Hypothesis ( ): An effect or difference exists. Significance Level ( ): Risk of rejecting null hypothesis when it’s true (Type I error). Test Statistic: Numerical value representing observed evidence against null hypothesis.

3.What is hypothesis testing in ML?

Statistical method to evaluate the performance and validity of machine learning models. Tests specific hypotheses about model behavior, like whether features influence predictions or if a model generalizes well to unseen data.

4.What is the difference between Pytest and hypothesis in Python?

Pytest purposes general testing framework for Python code while Hypothesis is a Property-based testing framework for Python, focusing on generating test cases based on specified properties of the code.

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Hypothesis Testing
Statistics Lecture 8.2: An Introduction to Hypothesis Testing
Concept of Statistical hypothesis

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Hypothesis Testing
Present the findings in your results and discussion section. Though the specific details might vary, the procedure you will use when testing a hypothesis will always follow some version of these steps. Table of contents. Step 1: State your null and alternate hypothesis. Step 2: Collect data. Step 3: Perform a statistical test.
7.1: Basics of Hypothesis Testing
Test Statistic: z = ¯ x − μo σ / √n since it is calculated as part of the testing of the hypothesis. Definition 7.1.4. p - value: probability that the test statistic will take on more extreme values than the observed test statistic, given that the null hypothesis is true.
Introduction to Hypothesis Testing
A hypothesis test consists of five steps: 1. State the hypotheses. State the null and alternative hypotheses. These two hypotheses need to be mutually exclusive, so if one is true then the other must be false. 2. Determine a significance level to use for the hypothesis. Decide on a significance level.
An Introduction to Statistics: Understanding Hypothesis Testing and
HYPOTHESIS TESTING. A clinical trial begins with an assumption or belief, and then proceeds to either prove or disprove this assumption. In statistical terms, this belief or assumption is known as a hypothesis. Counterintuitively, what the researcher believes in (or is trying to prove) is called the "alternate" hypothesis, and the opposite ...
9.1: Introduction to Hypothesis Testing
In hypothesis testing, the goal is to see if there is sufficient statistical evidence to reject a presumed null hypothesis in favor of a conjectured alternative hypothesis.The null hypothesis is usually denoted $H_0$ while the alternative hypothesis is usually denoted $H_1$. An hypothesis test is a statistical decision; the conclusion will either be to reject the null hypothesis in favor ...
Hypothesis to Be Tested: Definition and 4 Steps for Testing with Example
Hypothesis testing is an act in statistics whereby an analyst tests an assumption regarding a population parameter. The methodology employed by the analyst depends on the nature of the data used ...
3.1: The Fundamentals of Hypothesis Testing
Components of a Formal Hypothesis Test. The null hypothesis is a statement about the value of a population parameter, such as the population mean (µ) or the population proportion (p).It contains the condition of equality and is denoted as H 0 (H-naught).. H 0: µ = 157 or H0 : p = 0.37. The alternative hypothesis is the claim to be tested, the opposite of the null hypothesis.
Statistical Hypothesis Testing Overview
Hypothesis testing is a crucial procedure to perform when you want to make inferences about a population using a random sample. These inferences include estimating population properties such as the mean, differences between means, proportions, and the relationships between variables. This post provides an overview of statistical hypothesis testing.
S.3 Hypothesis Testing
S.3 Hypothesis Testing. In reviewing hypothesis tests, we start first with the general idea. Then, we keep returning to the basic procedures of hypothesis testing, each time adding a little more detail. The general idea of hypothesis testing involves: Making an initial assumption. Collecting evidence (data).
PDF Introduction to Hypothesis Testing
Hypothesis testing or significance testing is a method for testing a claim or hypothesis about a parameter in a population, using data measured in a sample. In this method, we test some hypothesis by determining the likelihood that a sample statistic could have been selected, if the hypothesis regarding the population parameter were true. The ...
Hypothesis Testing: Understanding the Basics, Types, and Importance
Hypothesis testing is a statistical method that helps to determine whether a hypothesis is true or not. It is a procedure that involves collecting and analyzing data to evaluate the probability of the null hypothesis being true. The null hypothesis is the hypothesis that there is no significant difference between a sample and the population.
Statistics
Hypothesis testing. Hypothesis testing is a form of statistical inference that uses data from a sample to draw conclusions about a population parameter or a population probability distribution.First, a tentative assumption is made about the parameter or distribution. This assumption is called the null hypothesis and is denoted by H 0.An alternative hypothesis (denoted H a), which is the ...
Hypothesis Testing: Definition, Uses, Limitations + Examples
Hypothesis Testing: Definition, Uses, Limitations + Examples; Hypothesis testing is as old as the scientific method and is at the heart of the research process. ... Also known as a basic hypothesis, a simple hypothesis suggests that an independent variable is responsible for a corresponding dependent variable. In other words, an occurrence of ...
Hypothesis Testing for 1 Sample: An Introduction
Here's one from a quick definition from the Stat Trek: A statistical hypothesis is an assumption about a population parameter. This assumption may or may not be true. Hypothesis testing refers to the formal procedures used by statisticians to accept or reject statistical hypotheses. ... The Basic Idea. Hypothesis tests show up in many areas ...
8.1.1: Introduction to Hypothesis Testing Part 1
The actual test begins by considering two hypotheses.They are called the null hypothesis and the alternative hypothesis.These hypotheses contain opposing viewpoints. $H_0$: The null hypothesis: It is a statement of no difference between the variables—they are not related. This can often be considered the status quo and as a result if you cannot accept the null it requires some action.
8.1: The Elements of Hypothesis Testing
Hypothesis testing is a statistical procedure in which a choice is made between a null hypothesis and an alternative hypothesis based on information in a sample. The end result of a hypotheses testing procedure is a choice of one of the following two possible conclusions: Reject H0 (and therefore accept Ha ), or.
Hypothesis Testing
Hypothesis Testing Definition. Hypothesis testing can be defined as a statistical tool that is used to identify if the results of an experiment are meaningful or not. It involves setting up a null hypothesis and an alternative hypothesis. ... The basic steps to perform hypothesis testing are as follows: Step 1: Set up the null hypothesis by ...
Understanding Hypothesis Testing
The process of hypothesis testing involves two hypotheses — a null hypothesis and an alternate hypothesis. The null hypothesis is a statement that assumes there is no relationship between two variables, no association between two groups or no change in the current situation — hence 'null'.
Significance tests (hypothesis testing)
Significance tests give us a formal process for using sample data to evaluate the likelihood of some claim about a population value. Learn how to conduct significance tests and calculate p-values to see how likely a sample result is to occur by random chance. You'll also see how we use p-values to make conclusions about hypotheses. Simple ...
What is Hypothesis Testing in Statistics? Types and Examples
Hypothesis testing is a statistical method used to determine if there is enough evidence in a sample data to draw conclusions about a population. It involves formulating two competing hypotheses, the null hypothesis (H0) and the alternative hypothesis (Ha), and then collecting data to assess the evidence.
1.4: Basic Concepts of Hypothesis Testing
One of the main goals of statistical hypothesis testing is to estimate the P P value, which is the probability of obtaining the observed results, or something more extreme, if the null hypothesis were true. If the observed results are unlikely under the null hypothesis, reject the null hypothesis. Alternatives to this "frequentist" approach to ...
Hypothesis Testing
Hypothesis testing in statistics refers to analyzing an assumption about a population parameter. It is used to make an educated guess about an assumption using statistics. With the use of sample data, hypothesis testing makes an assumption about how true the assumption is for the entire population from where the sample is being taken.
Understanding Hypothesis Testing
Hypothesis testing is a statistical method that is used to make a statistical decision using experimental data. Hypothesis testing is basically an assumption that we make about a population parameter. It evaluates two mutually exclusive statements about a population to determine which statement is best supported by the sample data.

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Hypothesis Testing | A Step-by-Step Guide with Easy Examples

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Rebecca Bevans

Introduction to Hypothesis Testing

The Two Types of Statistical Hypotheses

Hypothesis Tests

The Two Types of Decision Errors

One-Tailed and Two-Tailed Tests

Types of Hypothesis Tests

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An Introduction to Statistics: Understanding Hypothesis Testing and Statistical Errors

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SAMPLE VERSUS POPULATION

HYPOTHESIS TESTING

STATISTICAL ERRORS

Hypothesis to Be Tested: Definition and 4 Steps for Testing with Example

What Is Hypothesis Testing?

Key Takeaways

How Hypothesis Testing Works

4 Steps of Hypothesis Testing

Real-World Example of Hypothesis Testing

What is Hypothesis Testing?

What are the Four Key Steps Involved in Hypothesis Testing?

What are the Benefits of Hypothesis Testing?

What are the Limitations of Hypothesis Testing?

The Bottom Line

User Preferences

Keyboard Shortcuts

Example S.3.1

Example S.3.2

Errors in Hypothesis Testing Section

Making the Decision Section

In Practice

Hypothesis Testing: Understanding the Basics, Types, and Importance

What is a hypothesis?

Importance of Hypothesis Testing

Examples of Hypothesis Testing

Steps in Hypothesis Testing

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What is a Hypothesis?

What are the Types of Hypotheses?

2. Complex Hypothesis

3. Null Hypothesis

4. Alternative Hypothesis

5. Logical Hypothesis

6. Empirical Hypothesis

7. Statistical Hypothesis

What is Hypothesis Testing?

How Hypothesis Testing Works

What Are The Stages of Hypothesis Testing?

Applications of Hypothesis Testing in Research

What is an Example of Hypothesis Testing?

Importance/Benefits of Hypothesis Testing

Criticism and Limitations of Hypothesis Testing

Type I vs Type II Errors: Causes, Examples & Prevention

Internal Validity in Research: Definition, Threats, Examples

What is Pure or Basic Research? + [Examples & Method]

Alternative vs Null Hypothesis: Pros, Cons, Uses & Examples

Formplus - For Seamless Data Collection

What is a Hypothesis Test?

The Basic Idea

Some Examples of Hypothesis Tests

Example 2: Manual therapy in the treatment of carpal tunnel syndrome in diabetic patients: A randomized clinical trial

Example 3: Omega-3 fatty acids decreased irritability of patients with bipolar disorder in an add-on, open label study

Example 4: Evaluating the Efficacy of COVID-19 Vaccines

Example 5: Social Isolation During COVID-19 Pandemic. Perceived Stress and Containment Measures Compliance Among Polish and Italian Residents

Example 6: A Comparative Analysis of Student Performance in an Online vs. Face-to-Face Environmental Science Course From 2009 to 2016

But what does it all mean?

Key Terminology and Formulas

More About Hypotheses

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8.1: The Elements of Hypothesis Testing

Learning Objectives

Types of Hypotheses