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  • How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

  • An independent variable is something the researcher changes or controls.
  • A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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how to make a hypothesis in biology

Step 1. Ask a question

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

  • The relevant variables
  • The specific group being studied
  • The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

  • H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
  • H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.
Research question Hypothesis Null hypothesis
What are the health benefits of eating an apple a day? Increasing apple consumption in over-60s will result in decreasing frequency of doctor’s visits. Increasing apple consumption in over-60s will have no effect on frequency of doctor’s visits.
Which airlines have the most delays? Low-cost airlines are more likely to have delays than premium airlines. Low-cost and premium airlines are equally likely to have delays.
Can flexible work arrangements improve job satisfaction? Employees who have flexible working hours will report greater job satisfaction than employees who work fixed hours. There is no relationship between working hour flexibility and job satisfaction.
How effective is high school sex education at reducing teen pregnancies? Teenagers who received sex education lessons throughout high school will have lower rates of unplanned pregnancy teenagers who did not receive any sex education. High school sex education has no effect on teen pregnancy rates.
What effect does daily use of social media have on the attention span of under-16s? There is a negative between time spent on social media and attention span in under-16s. There is no relationship between social media use and attention span in under-16s.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

  • Sampling methods
  • Simple random sampling
  • Stratified sampling
  • Cluster sampling
  • Likert scales
  • Reproducibility

 Statistics

  • Null hypothesis
  • Statistical power
  • Probability distribution
  • Effect size
  • Poisson distribution

Research bias

  • Optimism bias
  • Cognitive bias
  • Implicit bias
  • Hawthorne effect
  • Anchoring bias
  • Explicit bias

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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How to Write a Hypothesis in 6 Steps, With Examples

Matt Ellis

A hypothesis is a statement that explains the predictions and reasoning of your research—an “educated guess” about how your scientific experiments will end. As a fundamental part of the scientific method, a good hypothesis is carefully written, but even the simplest ones can be difficult to put into words. 

Want to know how to write a hypothesis for your academic paper ? Below we explain the different types of hypotheses, what a good hypothesis requires, the steps to write your own, and plenty of examples.

Write with confidence Grammarly helps you polish your academic writing Write with Grammarly  

What is a hypothesis? 

One of our 10 essential words for university success , a hypothesis is one of the earliest stages of the scientific method. It’s essentially an educated guess—based on observations—of what the results of your experiment or research will be. 

Some hypothesis examples include:

  • If I water plants daily they will grow faster.
  • Adults can more accurately guess the temperature than children can. 
  • Butterflies prefer white flowers to orange ones.

If you’ve noticed that watering your plants every day makes them grow faster, your hypothesis might be “plants grow better with regular watering.” From there, you can begin experiments to test your hypothesis; in this example, you might set aside two plants, water one but not the other, and then record the results to see the differences. 

The language of hypotheses always discusses variables , or the elements that you’re testing. Variables can be objects, events, concepts, etc.—whatever is observable. 

There are two types of variables: independent and dependent. Independent variables are the ones that you change for your experiment, whereas dependent variables are the ones that you can only observe. In the above example, our independent variable is how often we water the plants and the dependent variable is how well they grow. 

Hypotheses determine the direction and organization of your subsequent research methods, and that makes them a big part of writing a research paper . Ultimately the reader wants to know whether your hypothesis was proven true or false, so it must be written clearly in the introduction and/or abstract of your paper. 

7 examples of hypotheses

Depending on the nature of your research and what you expect to find, your hypothesis will fall into one or more of the seven main categories. Keep in mind that these categories are not exclusive, so the same hypothesis might qualify as several different types. 

1 Simple hypothesis

A simple hypothesis suggests only the relationship between two variables: one independent and one dependent. 

  • If you stay up late, then you feel tired the next day. 
  • Turning off your phone makes it charge faster. 

2 Complex hypothesis

A complex hypothesis suggests the relationship between more than two variables, for example, two independents and one dependent, or vice versa. 

  • People who both (1) eat a lot of fatty foods and (2) have a family history of health problems are more likely to develop heart diseases. 
  • Older people who live in rural areas are happier than younger people who live in rural areas. 

3 Null hypothesis

A null hypothesis, abbreviated as H 0 , suggests that there is no relationship between variables. 

  • There is no difference in plant growth when using either bottled water or tap water. 
  • Professional psychics do not win the lottery more than other people. 

4 Alternative hypothesis

An alternative hypothesis, abbreviated as H 1 or H A , is used in conjunction with a null hypothesis. It states the opposite of the null hypothesis, so that one and only one must be true. 

  • Plants grow better with bottled water than tap water. 
  • Professional psychics win the lottery more than other people. 

5 Logical hypothesis

A logical hypothesis suggests a relationship between variables without actual evidence. Claims are instead based on reasoning or deduction, but lack actual data.  

  • An alien raised on Venus would have trouble breathing in Earth’s atmosphere. 
  • Dinosaurs with sharp, pointed teeth were probably carnivores. 

6 Empirical hypothesis

An empirical hypothesis, also known as a “working hypothesis,” is one that is currently being tested. Unlike logical hypotheses, empirical hypotheses rely on concrete data. 

  • Customers at restaurants will tip the same even if the wait staff’s base salary is raised. 
  • Washing your hands every hour can reduce the frequency of illness. 

7 Statistical hypothesis

A statistical hypothesis is when you test only a sample of a population and then apply statistical evidence to the results to draw a conclusion about the entire population. Instead of testing everything , you test only a portion and generalize the rest based on preexisting data. 

  • In humans, the birth-gender ratio of males to females is 1.05 to 1.00.  
  • Approximately 2% of the world population has natural red hair. 

What makes a good hypothesis?

No matter what you’re testing, a good hypothesis is written according to the same guidelines. In particular, keep these five characteristics in mind: 

Cause and effect

Hypotheses always include a cause-and-effect relationship where one variable causes another to change (or not change if you’re using a null hypothesis). This can best be reflected as an if-then statement: If one variable occurs, then another variable changes. 

Testable prediction

Most hypotheses are designed to be tested (with the exception of logical hypotheses). Before committing to a hypothesis, make sure you’re actually able to conduct experiments on it. Choose a testable hypothesis with an independent variable that you have absolute control over. 

Independent and dependent variables

Define your variables in your hypothesis so your readers understand the big picture. You don’t have to specifically say which ones are independent and dependent variables, but you definitely want to mention them all. 

Candid language

Writing can easily get convoluted, so make sure your hypothesis remains as simple and clear as possible. Readers use your hypothesis as a contextual pillar to unify your entire paper, so there should be no confusion or ambiguity. If you’re unsure about your phrasing, try reading your hypothesis to a friend to see if they understand. 

Adherence to ethics

It’s not always about what you can test, but what you should test. Avoid hypotheses that require questionable or taboo experiments to keep ethics (and therefore, credibility) intact.

How to write a hypothesis in 6 steps

1 ask a question.

Curiosity has inspired some of history’s greatest scientific achievements, so a good place to start is to ask yourself questions about the world around you. Why are things the way they are? What causes the factors you see around you? If you can, choose a research topic that you’re interested in so your curiosity comes naturally. 

2 Conduct preliminary research

Next, collect some background information on your topic. How much background information you need depends on what you’re attempting. It could require reading several books, or it could be as simple as performing a web search for a quick answer. You don’t necessarily have to prove or disprove your hypothesis at this stage; rather, collect only what you need to prove or disprove it yourself. 

3 Define your variables

Once you have an idea of what your hypothesis will be, select which variables are independent and which are dependent. Remember that independent variables can only be factors that you have absolute control over, so consider the limits of your experiment before finalizing your hypothesis. 

4 Phrase it as an if-then statement

When writing a hypothesis, it helps to phrase it using an if-then format, such as, “ If I water a plant every day, then it will grow better.” This format can get tricky when dealing with multiple variables, but in general, it’s a reliable method for expressing the cause-and-effect relationship you’re testing. 

5  Collect data to support your hypothesis

A hypothesis is merely a means to an end. The priority of any scientific research is the conclusion. Once you have your hypothesis laid out and your variables chosen, you can then begin your experiments. Ideally, you’ll collect data to support your hypothesis, but don’t worry if your research ends up proving it wrong—that’s all part of the scientific method. 

6 Write with confidence

Last, you’ll want to record your findings in a research paper for others to see. This requires a bit of writing know-how, quite a different skill set than conducting experiments. 

That’s where Grammarly can be a major help; our writing suggestions point out not only grammar and spelling mistakes , but also new word choices and better phrasing. While you write, Grammarly automatically recommends optimal language and highlights areas where readers might get confused, ensuring that your hypothesis—and your final paper—are clear and polished.

how to make a hypothesis in biology

Module 1: Introduction to Biology

Experiments and hypotheses, learning outcomes.

  • Form a hypothesis and use it to design a scientific experiment

Now we’ll focus on the methods of scientific inquiry. Science often involves making observations and developing hypotheses. Experiments and further observations are often used to test the hypotheses.

A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always. For example, a scientist studying the mating behaviors of ladybugs might begin with detailed observations of ladybugs mating in their natural habitats. While this research may not be experimental, it is scientific: it involves careful and verifiable observation of the natural world. The same scientist might then treat some of the ladybugs with a hormone hypothesized to trigger mating and observe whether these ladybugs mated sooner or more often than untreated ones. This would qualify as an experiment because the scientist is now making a change in the system and observing the effects.

Forming a Hypothesis

When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis through observations and research, and it must be possible to prove your hypothesis false.

For example, Michael observes that maple trees lose their leaves in the fall. He might then propose a possible explanation for this observation: “cold weather causes maple trees to lose their leaves in the fall.” This statement is testable. He could grow maple trees in a warm enclosed environment such as a greenhouse and see if their leaves still dropped in the fall. The hypothesis is also falsifiable. If the leaves still dropped in the warm environment, then clearly temperature was not the main factor in causing maple leaves to drop in autumn.

In the Try It below, you can practice recognizing scientific hypotheses. As you consider each statement, try to think as a scientist would: can I test this hypothesis with observations or experiments? Is the statement falsifiable? If the answer to either of these questions is “no,” the statement is not a valid scientific hypothesis.

Practice Questions

Determine whether each following statement is a scientific hypothesis.

Air pollution from automobile exhaust can trigger symptoms in people with asthma.

  • No. This statement is not testable or falsifiable.
  • No. This statement is not testable.
  • No. This statement is not falsifiable.
  • Yes. This statement is testable and falsifiable.

Natural disasters, such as tornadoes, are punishments for bad thoughts and behaviors.

a: No. This statement is not testable or falsifiable. “Bad thoughts and behaviors” are excessively vague and subjective variables that would be impossible to measure or agree upon in a reliable way. The statement might be “falsifiable” if you came up with a counterexample: a “wicked” place that was not punished by a natural disaster. But some would question whether the people in that place were really wicked, and others would continue to predict that a natural disaster was bound to strike that place at some point. There is no reason to suspect that people’s immoral behavior affects the weather unless you bring up the intervention of a supernatural being, making this idea even harder to test.

Testing a Vaccine

Let’s examine the scientific process by discussing an actual scientific experiment conducted by researchers at the University of Washington. These researchers investigated whether a vaccine may reduce the incidence of the human papillomavirus (HPV). The experimental process and results were published in an article titled, “ A controlled trial of a human papillomavirus type 16 vaccine .”

Preliminary observations made by the researchers who conducted the HPV experiment are listed below:

  • Human papillomavirus (HPV) is the most common sexually transmitted virus in the United States.
  • There are about 40 different types of HPV. A significant number of people that have HPV are unaware of it because many of these viruses cause no symptoms.
  • Some types of HPV can cause cervical cancer.
  • About 4,000 women a year die of cervical cancer in the United States.

Practice Question

Researchers have developed a potential vaccine against HPV and want to test it. What is the first testable hypothesis that the researchers should study?

  • HPV causes cervical cancer.
  • People should not have unprotected sex with many partners.
  • People who get the vaccine will not get HPV.
  • The HPV vaccine will protect people against cancer.

Experimental Design

You’ve successfully identified a hypothesis for the University of Washington’s study on HPV: People who get the HPV vaccine will not get HPV.

The next step is to design an experiment that will test this hypothesis. There are several important factors to consider when designing a scientific experiment. First, scientific experiments must have an experimental group. This is the group that receives the experimental treatment necessary to address the hypothesis.

The experimental group receives the vaccine, but how can we know if the vaccine made a difference? Many things may change HPV infection rates in a group of people over time. To clearly show that the vaccine was effective in helping the experimental group, we need to include in our study an otherwise similar control group that does not get the treatment. We can then compare the two groups and determine if the vaccine made a difference. The control group shows us what happens in the absence of the factor under study.

However, the control group cannot get “nothing.” Instead, the control group often receives a placebo. A placebo is a procedure that has no expected therapeutic effect—such as giving a person a sugar pill or a shot containing only plain saline solution with no drug. Scientific studies have shown that the “placebo effect” can alter experimental results because when individuals are told that they are or are not being treated, this knowledge can alter their actions or their emotions, which can then alter the results of the experiment.

Moreover, if the doctor knows which group a patient is in, this can also influence the results of the experiment. Without saying so directly, the doctor may show—through body language or other subtle cues—their views about whether the patient is likely to get well. These errors can then alter the patient’s experience and change the results of the experiment. Therefore, many clinical studies are “double blind.” In these studies, neither the doctor nor the patient knows which group the patient is in until all experimental results have been collected.

Both placebo treatments and double-blind procedures are designed to prevent bias. Bias is any systematic error that makes a particular experimental outcome more or less likely. Errors can happen in any experiment: people make mistakes in measurement, instruments fail, computer glitches can alter data. But most such errors are random and don’t favor one outcome over another. Patients’ belief in a treatment can make it more likely to appear to “work.” Placebos and double-blind procedures are used to level the playing field so that both groups of study subjects are treated equally and share similar beliefs about their treatment.

The scientists who are researching the effectiveness of the HPV vaccine will test their hypothesis by separating 2,392 young women into two groups: the control group and the experimental group. Answer the following questions about these two groups.

  • This group is given a placebo.
  • This group is deliberately infected with HPV.
  • This group is given nothing.
  • This group is given the HPV vaccine.
  • a: This group is given a placebo. A placebo will be a shot, just like the HPV vaccine, but it will have no active ingredient. It may change peoples’ thinking or behavior to have such a shot given to them, but it will not stimulate the immune systems of the subjects in the same way as predicted for the vaccine itself.
  • d: This group is given the HPV vaccine. The experimental group will receive the HPV vaccine and researchers will then be able to see if it works, when compared to the control group.

Experimental Variables

A variable is a characteristic of a subject (in this case, of a person in the study) that can vary over time or among individuals. Sometimes a variable takes the form of a category, such as male or female; often a variable can be measured precisely, such as body height. Ideally, only one variable is different between the control group and the experimental group in a scientific experiment. Otherwise, the researchers will not be able to determine which variable caused any differences seen in the results. For example, imagine that the people in the control group were, on average, much more sexually active than the people in the experimental group. If, at the end of the experiment, the control group had a higher rate of HPV infection, could you confidently determine why? Maybe the experimental subjects were protected by the vaccine, but maybe they were protected by their low level of sexual contact.

To avoid this situation, experimenters make sure that their subject groups are as similar as possible in all variables except for the variable that is being tested in the experiment. This variable, or factor, will be deliberately changed in the experimental group. The one variable that is different between the two groups is called the independent variable. An independent variable is known or hypothesized to cause some outcome. Imagine an educational researcher investigating the effectiveness of a new teaching strategy in a classroom. The experimental group receives the new teaching strategy, while the control group receives the traditional strategy. It is the teaching strategy that is the independent variable in this scenario. In an experiment, the independent variable is the variable that the scientist deliberately changes or imposes on the subjects.

Dependent variables are known or hypothesized consequences; they are the effects that result from changes or differences in an independent variable. In an experiment, the dependent variables are those that the scientist measures before, during, and particularly at the end of the experiment to see if they have changed as expected. The dependent variable must be stated so that it is clear how it will be observed or measured. Rather than comparing “learning” among students (which is a vague and difficult to measure concept), an educational researcher might choose to compare test scores, which are very specific and easy to measure.

In any real-world example, many, many variables MIGHT affect the outcome of an experiment, yet only one or a few independent variables can be tested. Other variables must be kept as similar as possible between the study groups and are called control variables . For our educational research example, if the control group consisted only of people between the ages of 18 and 20 and the experimental group contained people between the ages of 30 and 35, we would not know if it was the teaching strategy or the students’ ages that played a larger role in the results. To avoid this problem, a good study will be set up so that each group contains students with a similar age profile. In a well-designed educational research study, student age will be a controlled variable, along with other possibly important factors like gender, past educational achievement, and pre-existing knowledge of the subject area.

What is the independent variable in this experiment?

  • Sex (all of the subjects will be female)
  • Presence or absence of the HPV vaccine
  • Presence or absence of HPV (the virus)

List three control variables other than age.

What is the dependent variable in this experiment?

  • Sex (male or female)
  • Rates of HPV infection
  • Age (years)
  • Revision and adaptation. Authored by : Shelli Carter and Lumen Learning. Provided by : Lumen Learning. License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike
  • Scientific Inquiry. Provided by : Open Learning Initiative. Located at : https://oli.cmu.edu/jcourse/workbook/activity/page?context=434a5c2680020ca6017c03488572e0f8 . Project : Introduction to Biology (Open + Free). License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike

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Scientific Method: Step 3: HYPOTHESIS

  • Step 1: QUESTION
  • Step 2: RESEARCH
  • Step 3: HYPOTHESIS
  • Step 4: EXPERIMENT
  • Step 5: DATA
  • Step 6: CONCLUSION

Step 3: State your hypothesis

Now it's time to state your hypothesis . The hypothesis is an educated guess as to what will happen during your experiment. 

The hypothesis is often written using the words "IF" and "THEN." For example, " If I do not study, then I will fail the test." The "if' and "then" statements reflect your independent and dependent variables . 

The hypothesis should relate back to your original question and must be testable .

A word about variables...

Your experiment will include variables to measure and to explain any cause and effect. Below you will find some useful links describing the different types of variables.

  • "What are independent and dependent variables" NCES
  • [VIDEO] Biology: Independent vs. Dependent Variables (Nucleus Medical Media) Video explaining independent and dependent variables, with examples.

Resource Links

  • What is and How to Write a Good Hypothesis in Research? (Elsevier)
  • Hypothesis brochure from Penn State/Berks

  • << Previous: Step 2: RESEARCH
  • Next: Step 4: EXPERIMENT >>
  • Last Updated: Aug 26, 2024 10:04 AM
  • URL: https://harford.libguides.com/scientific_method
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Step-by-Step Guide: How to Craft a Strong Research Hypothesis

  • 4 minute read

Table of Contents

A research hypothesis is a concise statement about the expected result of an experiment or project. In many ways, a research hypothesis represents the starting point for a scientific endeavor, as it establishes a tentative assumption that is eventually substantiated or falsified, ultimately improving our certainty about the subject investigated.   

To help you with this and ease the process, in this article, we discuss the purpose of research hypotheses and list the most essential qualities of a compelling hypothesis. Let’s find out!  

How to Craft a Research Hypothesis  

Crafting a research hypothesis begins with a comprehensive literature review to identify a knowledge gap in your field. Once you find a question or problem, come up with a possible answer or explanation, which becomes your hypothesis. Now think about the specific methods of experimentation that can prove or disprove the hypothesis, which ultimately lead to the results of the study.   

Enlisted below are some standard formats in which you can formulate a hypothesis¹ :  

  • A hypothesis can use the if/then format when it seeks to explore the correlation between two variables in a study primarily.  

Example: If administered drug X, then patients will experience reduced fatigue from cancer treatment.  

  • A hypothesis can adopt when X/then Y format when it primarily aims to expose a connection between two variables  

Example: When workers spend a significant portion of their waking hours in sedentary work , then they experience a greater frequency of digestive problems.  

  • A hypothesis can also take the form of a direct statement.  

Example: Drug X and drug Y reduce the risk of cognitive decline through the same chemical pathways  

What are the Features of an Effective Hypothesis?  

Hypotheses in research need to satisfy specific criteria to be considered scientifically rigorous. Here are the most notable qualities of a strong hypothesis:  

  • Testability: Ensure the hypothesis allows you to work towards observable and testable results.  
  • Brevity and objectivity: Present your hypothesis as a brief statement and avoid wordiness.  
  • Clarity and Relevance: The hypothesis should reflect a clear idea of what we know and what we expect to find out about a phenomenon and address the significant knowledge gap relevant to a field of study.   

Understanding Null and Alternative Hypotheses in Research  

There are two types of hypotheses used commonly in research that aid statistical analyses. These are known as the null hypothesis and the alternative hypothesis . A null hypothesis is a statement assumed to be factual in the initial phase of the study.   

For example, if a researcher is testing the efficacy of a new drug, then the null hypothesis will posit that the drug has no benefits compared to an inactive control or placebo . Suppose the data collected through a drug trial leads a researcher to reject the null hypothesis. In that case, it is considered to substantiate the alternative hypothesis in the above example, that the new drug provides benefits compared to the placebo.  

Let’s take a closer look at the null hypothesis and alternative hypothesis with two more examples:  

Null Hypothesis:  

The rate of decline in the number of species in habitat X in the last year is the same as in the last 100 years when controlled for all factors except the recent wildfires.  

In the next experiment, the researcher will experimentally reject this null hypothesis in order to confirm the following alternative hypothesis :  

The rate of decline in the number of species in habitat X in the last year is different from the rate of decline in the last 100 years when controlled for all factors other than the recent wildfires.  

In the pair of null and alternative hypotheses stated above, a statistical comparison of the rate of species decline over a century and the preceding year will help the research experimentally test the null hypothesis, helping to draw scientifically valid conclusions about two factors—wildfires and species decline.   

We also recommend that researchers pay attention to contextual echoes and connections when writing research hypotheses. Research hypotheses are often closely linked to the introduction ² , such as the context of the study, and can similarly influence the reader’s judgment of the relevance and validity of the research hypothesis.  

Seasoned experts, such as professionals at Elsevier Language Services, guide authors on how to best embed a hypothesis within an article so that it communicates relevance and credibility. Contact us if you want help in ensuring readers find your hypothesis robust and unbiased.  

References  

  • Hypotheses – The University Writing Center. (n.d.). https://writingcenter.tamu.edu/writing-speaking-guides/hypotheses  
  • Shaping the research question and hypothesis. (n.d.). Students. https://students.unimelb.edu.au/academic-skills/graduate-research-services/writing-thesis-sections-part-2/shaping-the-research-question-and-hypothesis  

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How to Write a Strong Hypothesis | Guide & Examples

Published on 6 May 2022 by Shona McCombes .

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more variables . An independent variable is something the researcher changes or controls. A dependent variable is something the researcher observes and measures.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

Prevent plagiarism, run a free check.

Step 1: ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2: Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalise more complex constructs.

Step 3: Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

Step 4: Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

  • The relevant variables
  • The specific group being studied
  • The predicted outcome of the experiment or analysis

Step 5: Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

Step 6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis. The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

Research question Hypothesis Null hypothesis
What are the health benefits of eating an apple a day? Increasing apple consumption in over-60s will result in decreasing frequency of doctor’s visits. Increasing apple consumption in over-60s will have no effect on frequency of doctor’s visits.
Which airlines have the most delays? Low-cost airlines are more likely to have delays than premium airlines. Low-cost and premium airlines are equally likely to have delays.
Can flexible work arrangements improve job satisfaction? Employees who have flexible working hours will report greater job satisfaction than employees who work fixed hours. There is no relationship between working hour flexibility and job satisfaction.
How effective is secondary school sex education at reducing teen pregnancies? Teenagers who received sex education lessons throughout secondary school will have lower rates of unplanned pregnancy than teenagers who did not receive any sex education. Secondary school sex education has no effect on teen pregnancy rates.
What effect does daily use of social media have on the attention span of under-16s? There is a negative correlation between time spent on social media and attention span in under-16s. There is no relationship between social media use and attention span in under-16s.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis is not just a guess. It should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Formulating Hypotheses for Different Study Designs

Durga prasanna misra.

1 Department of Clinical Immunology and Rheumatology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.

Armen Yuri Gasparyan

2 Departments of Rheumatology and Research and Development, Dudley Group NHS Foundation Trust (Teaching Trust of the University of Birmingham, UK), Russells Hall Hospital, Dudley, UK.

Olena Zimba

3 Department of Internal Medicine #2, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine.

Marlen Yessirkepov

4 Department of Biology and Biochemistry, South Kazakhstan Medical Academy, Shymkent, Kazakhstan.

Vikas Agarwal

George d. kitas.

5 Centre for Epidemiology versus Arthritis, University of Manchester, Manchester, UK.

Generating a testable working hypothesis is the first step towards conducting original research. Such research may prove or disprove the proposed hypothesis. Case reports, case series, online surveys and other observational studies, clinical trials, and narrative reviews help to generate hypotheses. Observational and interventional studies help to test hypotheses. A good hypothesis is usually based on previous evidence-based reports. Hypotheses without evidence-based justification and a priori ideas are not received favourably by the scientific community. Original research to test a hypothesis should be carefully planned to ensure appropriate methodology and adequate statistical power. While hypotheses can challenge conventional thinking and may be controversial, they should not be destructive. A hypothesis should be tested by ethically sound experiments with meaningful ethical and clinical implications. The coronavirus disease 2019 pandemic has brought into sharp focus numerous hypotheses, some of which were proven (e.g. effectiveness of corticosteroids in those with hypoxia) while others were disproven (e.g. ineffectiveness of hydroxychloroquine and ivermectin).

Graphical Abstract

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DEFINING WORKING AND STANDALONE SCIENTIFIC HYPOTHESES

Science is the systematized description of natural truths and facts. Routine observations of existing life phenomena lead to the creative thinking and generation of ideas about mechanisms of such phenomena and related human interventions. Such ideas presented in a structured format can be viewed as hypotheses. After generating a hypothesis, it is necessary to test it to prove its validity. Thus, hypothesis can be defined as a proposed mechanism of a naturally occurring event or a proposed outcome of an intervention. 1 , 2

Hypothesis testing requires choosing the most appropriate methodology and adequately powering statistically the study to be able to “prove” or “disprove” it within predetermined and widely accepted levels of certainty. This entails sample size calculation that often takes into account previously published observations and pilot studies. 2 , 3 In the era of digitization, hypothesis generation and testing may benefit from the availability of numerous platforms for data dissemination, social networking, and expert validation. Related expert evaluations may reveal strengths and limitations of proposed ideas at early stages of post-publication promotion, preventing the implementation of unsupported controversial points. 4

Thus, hypothesis generation is an important initial step in the research workflow, reflecting accumulating evidence and experts' stance. In this article, we overview the genesis and importance of scientific hypotheses and their relevance in the era of the coronavirus disease 2019 (COVID-19) pandemic.

DO WE NEED HYPOTHESES FOR ALL STUDY DESIGNS?

Broadly, research can be categorized as primary or secondary. In the context of medicine, primary research may include real-life observations of disease presentations and outcomes. Single case descriptions, which often lead to new ideas and hypotheses, serve as important starting points or justifications for case series and cohort studies. The importance of case descriptions is particularly evident in the context of the COVID-19 pandemic when unique, educational case reports have heralded a new era in clinical medicine. 5

Case series serve similar purpose to single case reports, but are based on a slightly larger quantum of information. Observational studies, including online surveys, describe the existing phenomena at a larger scale, often involving various control groups. Observational studies include variable-scale epidemiological investigations at different time points. Interventional studies detail the results of therapeutic interventions.

Secondary research is based on already published literature and does not directly involve human or animal subjects. Review articles are generated by secondary research. These could be systematic reviews which follow methods akin to primary research but with the unit of study being published papers rather than humans or animals. Systematic reviews have a rigid structure with a mandatory search strategy encompassing multiple databases, systematic screening of search results against pre-defined inclusion and exclusion criteria, critical appraisal of study quality and an optional component of collating results across studies quantitatively to derive summary estimates (meta-analysis). 6 Narrative reviews, on the other hand, have a more flexible structure. Systematic literature searches to minimise bias in selection of articles are highly recommended but not mandatory. 7 Narrative reviews are influenced by the authors' viewpoint who may preferentially analyse selected sets of articles. 8

In relation to primary research, case studies and case series are generally not driven by a working hypothesis. Rather, they serve as a basis to generate a hypothesis. Observational or interventional studies should have a hypothesis for choosing research design and sample size. The results of observational and interventional studies further lead to the generation of new hypotheses, testing of which forms the basis of future studies. Review articles, on the other hand, may not be hypothesis-driven, but form fertile ground to generate future hypotheses for evaluation. Fig. 1 summarizes which type of studies are hypothesis-driven and which lead on to hypothesis generation.

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STANDARDS OF WORKING AND SCIENTIFIC HYPOTHESES

A review of the published literature did not enable the identification of clearly defined standards for working and scientific hypotheses. It is essential to distinguish influential versus not influential hypotheses, evidence-based hypotheses versus a priori statements and ideas, ethical versus unethical, or potentially harmful ideas. The following points are proposed for consideration while generating working and scientific hypotheses. 1 , 2 Table 1 summarizes these points.

Points to be considered while evaluating the validity of hypotheses
Backed by evidence-based data
Testable by relevant study designs
Supported by preliminary (pilot) studies
Testable by ethical studies
Maintaining a balance between scientific temper and controversy

Evidence-based data

A scientific hypothesis should have a sound basis on previously published literature as well as the scientist's observations. Randomly generated (a priori) hypotheses are unlikely to be proven. A thorough literature search should form the basis of a hypothesis based on published evidence. 7

Unless a scientific hypothesis can be tested, it can neither be proven nor be disproven. Therefore, a scientific hypothesis should be amenable to testing with the available technologies and the present understanding of science.

Supported by pilot studies

If a hypothesis is based purely on a novel observation by the scientist in question, it should be grounded on some preliminary studies to support it. For example, if a drug that targets a specific cell population is hypothesized to be useful in a particular disease setting, then there must be some preliminary evidence that the specific cell population plays a role in driving that disease process.

Testable by ethical studies

The hypothesis should be testable by experiments that are ethically acceptable. 9 For example, a hypothesis that parachutes reduce mortality from falls from an airplane cannot be tested using a randomized controlled trial. 10 This is because it is obvious that all those jumping from a flying plane without a parachute would likely die. Similarly, the hypothesis that smoking tobacco causes lung cancer cannot be tested by a clinical trial that makes people take up smoking (since there is considerable evidence for the health hazards associated with smoking). Instead, long-term observational studies comparing outcomes in those who smoke and those who do not, as was performed in the landmark epidemiological case control study by Doll and Hill, 11 are more ethical and practical.

Balance between scientific temper and controversy

Novel findings, including novel hypotheses, particularly those that challenge established norms, are bound to face resistance for their wider acceptance. Such resistance is inevitable until the time such findings are proven with appropriate scientific rigor. However, hypotheses that generate controversy are generally unwelcome. For example, at the time the pandemic of human immunodeficiency virus (HIV) and AIDS was taking foot, there were numerous deniers that refused to believe that HIV caused AIDS. 12 , 13 Similarly, at a time when climate change is causing catastrophic changes to weather patterns worldwide, denial that climate change is occurring and consequent attempts to block climate change are certainly unwelcome. 14 The denialism and misinformation during the COVID-19 pandemic, including unfortunate examples of vaccine hesitancy, are more recent examples of controversial hypotheses not backed by science. 15 , 16 An example of a controversial hypothesis that was a revolutionary scientific breakthrough was the hypothesis put forth by Warren and Marshall that Helicobacter pylori causes peptic ulcers. Initially, the hypothesis that a microorganism could cause gastritis and gastric ulcers faced immense resistance. When the scientists that proposed the hypothesis themselves ingested H. pylori to induce gastritis in themselves, only then could they convince the wider world about their hypothesis. Such was the impact of the hypothesis was that Barry Marshall and Robin Warren were awarded the Nobel Prize in Physiology or Medicine in 2005 for this discovery. 17 , 18

DISTINGUISHING THE MOST INFLUENTIAL HYPOTHESES

Influential hypotheses are those that have stood the test of time. An archetype of an influential hypothesis is that proposed by Edward Jenner in the eighteenth century that cowpox infection protects against smallpox. While this observation had been reported for nearly a century before this time, it had not been suitably tested and publicised until Jenner conducted his experiments on a young boy by demonstrating protection against smallpox after inoculation with cowpox. 19 These experiments were the basis for widespread smallpox immunization strategies worldwide in the 20th century which resulted in the elimination of smallpox as a human disease today. 20

Other influential hypotheses are those which have been read and cited widely. An example of this is the hygiene hypothesis proposing an inverse relationship between infections in early life and allergies or autoimmunity in adulthood. An analysis reported that this hypothesis had been cited more than 3,000 times on Scopus. 1

LESSONS LEARNED FROM HYPOTHESES AMIDST THE COVID-19 PANDEMIC

The COVID-19 pandemic devastated the world like no other in recent memory. During this period, various hypotheses emerged, understandably so considering the public health emergency situation with innumerable deaths and suffering for humanity. Within weeks of the first reports of COVID-19, aberrant immune system activation was identified as a key driver of organ dysfunction and mortality in this disease. 21 Consequently, numerous drugs that suppress the immune system or abrogate the activation of the immune system were hypothesized to have a role in COVID-19. 22 One of the earliest drugs hypothesized to have a benefit was hydroxychloroquine. Hydroxychloroquine was proposed to interfere with Toll-like receptor activation and consequently ameliorate the aberrant immune system activation leading to pathology in COVID-19. 22 The drug was also hypothesized to have a prophylactic role in preventing infection or disease severity in COVID-19. It was also touted as a wonder drug for the disease by many prominent international figures. However, later studies which were well-designed randomized controlled trials failed to demonstrate any benefit of hydroxychloroquine in COVID-19. 23 , 24 , 25 , 26 Subsequently, azithromycin 27 , 28 and ivermectin 29 were hypothesized as potential therapies for COVID-19, but were not supported by evidence from randomized controlled trials. The role of vitamin D in preventing disease severity was also proposed, but has not been proven definitively until now. 30 , 31 On the other hand, randomized controlled trials identified the evidence supporting dexamethasone 32 and interleukin-6 pathway blockade with tocilizumab as effective therapies for COVID-19 in specific situations such as at the onset of hypoxia. 33 , 34 Clues towards the apparent effectiveness of various drugs against severe acute respiratory syndrome coronavirus 2 in vitro but their ineffectiveness in vivo have recently been identified. Many of these drugs are weak, lipophilic bases and some others induce phospholipidosis which results in apparent in vitro effectiveness due to non-specific off-target effects that are not replicated inside living systems. 35 , 36

Another hypothesis proposed was the association of the routine policy of vaccination with Bacillus Calmette-Guerin (BCG) with lower deaths due to COVID-19. This hypothesis emerged in the middle of 2020 when COVID-19 was still taking foot in many parts of the world. 37 , 38 Subsequently, many countries which had lower deaths at that time point went on to have higher numbers of mortality, comparable to other areas of the world. Furthermore, the hypothesis that BCG vaccination reduced COVID-19 mortality was a classic example of ecological fallacy. Associations between population level events (ecological studies; in this case, BCG vaccination and COVID-19 mortality) cannot be directly extrapolated to the individual level. Furthermore, such associations cannot per se be attributed as causal in nature, and can only serve to generate hypotheses that need to be tested at the individual level. 39

IS TRADITIONAL PEER REVIEW EFFICIENT FOR EVALUATION OF WORKING AND SCIENTIFIC HYPOTHESES?

Traditionally, publication after peer review has been considered the gold standard before any new idea finds acceptability amongst the scientific community. Getting a work (including a working or scientific hypothesis) reviewed by experts in the field before experiments are conducted to prove or disprove it helps to refine the idea further as well as improve the experiments planned to test the hypothesis. 40 A route towards this has been the emergence of journals dedicated to publishing hypotheses such as the Central Asian Journal of Medical Hypotheses and Ethics. 41 Another means of publishing hypotheses is through registered research protocols detailing the background, hypothesis, and methodology of a particular study. If such protocols are published after peer review, then the journal commits to publishing the completed study irrespective of whether the study hypothesis is proven or disproven. 42 In the post-pandemic world, online research methods such as online surveys powered via social media channels such as Twitter and Instagram might serve as critical tools to generate as well as to preliminarily test the appropriateness of hypotheses for further evaluation. 43 , 44

Some radical hypotheses might be difficult to publish after traditional peer review. These hypotheses might only be acceptable by the scientific community after they are tested in research studies. Preprints might be a way to disseminate such controversial and ground-breaking hypotheses. 45 However, scientists might prefer to keep their hypotheses confidential for the fear of plagiarism of ideas, avoiding online posting and publishing until they have tested the hypotheses.

SUGGESTIONS ON GENERATING AND PUBLISHING HYPOTHESES

Publication of hypotheses is important, however, a balance is required between scientific temper and controversy. Journal editors and reviewers might keep in mind these specific points, summarized in Table 2 and detailed hereafter, while judging the merit of hypotheses for publication. Keeping in mind the ethical principle of primum non nocere, a hypothesis should be published only if it is testable in a manner that is ethically appropriate. 46 Such hypotheses should be grounded in reality and lend themselves to further testing to either prove or disprove them. It must be considered that subsequent experiments to prove or disprove a hypothesis have an equal chance of failing or succeeding, akin to tossing a coin. A pre-conceived belief that a hypothesis is unlikely to be proven correct should not form the basis of rejection of such a hypothesis for publication. In this context, hypotheses generated after a thorough literature search to identify knowledge gaps or based on concrete clinical observations on a considerable number of patients (as opposed to random observations on a few patients) are more likely to be acceptable for publication by peer-reviewed journals. Also, hypotheses should be considered for publication or rejection based on their implications for science at large rather than whether the subsequent experiments to test them end up with results in favour of or against the original hypothesis.

Points to be considered before a hypothesis is acceptable for publication
Experiments required to test hypotheses should be ethically acceptable as per the World Medical Association declaration on ethics and related statements
Pilot studies support hypotheses
Single clinical observations and expert opinion surveys may support hypotheses
Testing hypotheses requires robust methodology and statistical power
Hypotheses that challenge established views and concepts require proper evidence-based justification

Hypotheses form an important part of the scientific literature. The COVID-19 pandemic has reiterated the importance and relevance of hypotheses for dealing with public health emergencies and highlighted the need for evidence-based and ethical hypotheses. A good hypothesis is testable in a relevant study design, backed by preliminary evidence, and has positive ethical and clinical implications. General medical journals might consider publishing hypotheses as a specific article type to enable more rapid advancement of science.

Disclosure: The authors have no potential conflicts of interest to disclose.

Author Contributions:

  • Data curation: Gasparyan AY, Misra DP, Zimba O, Yessirkepov M, Agarwal V, Kitas GD.

Hypothesis n., plural: hypotheses [/haɪˈpɑːθəsɪs/] Definition: Testable scientific prediction

Table of Contents

What Is Hypothesis?

A scientific hypothesis is a foundational element of the scientific method . It’s a testable statement proposing a potential explanation for natural phenomena. The term hypothesis means “little theory” . A hypothesis is a short statement that can be tested and gives a possible reason for a phenomenon or a possible link between two variables . In the setting of scientific research, a hypothesis is a tentative explanation or statement that can be proven wrong and is used to guide experiments and empirical research.

It is an important part of the scientific method because it gives a basis for planning tests, gathering data, and judging evidence to see if it is true and could help us understand how natural things work. Several hypotheses can be tested in the real world, and the results of careful and systematic observation and analysis can be used to support, reject, or improve them.

Researchers and scientists often use the word hypothesis to refer to this educated guess . These hypotheses are firmly established based on scientific principles and the rigorous testing of new technology and experiments .

For example, in astrophysics, the Big Bang Theory is a working hypothesis that explains the origins of the universe and considers it as a natural phenomenon. It is among the most prominent scientific hypotheses in the field.

“The scientific method: steps, terms, and examples” by Scishow:

Biology definition: A hypothesis  is a supposition or tentative explanation for (a group of) phenomena, (a set of) facts, or a scientific inquiry that may be tested, verified or answered by further investigation or methodological experiment. It is like a scientific guess . It’s an idea or prediction that scientists make before they do experiments. They use it to guess what might happen and then test it to see if they were right. It’s like a smart guess that helps them learn new things. A scientific hypothesis that has been verified through scientific experiment and research may well be considered a scientific theory .

Etymology: The word “hypothesis” comes from the Greek word “hupothesis,” which means “a basis” or “a supposition.” It combines “hupo” (under) and “thesis” (placing). Synonym:   proposition; assumption; conjecture; postulate Compare:   theory See also: null hypothesis

Characteristics Of Hypothesis

A useful hypothesis must have the following qualities:

  • It should never be written as a question.
  • You should be able to test it in the real world to see if it’s right or wrong.
  • It needs to be clear and exact.
  • It should list the factors that will be used to figure out the relationship.
  • It should only talk about one thing. You can make a theory in either a descriptive or form of relationship.
  • It shouldn’t go against any natural rule that everyone knows is true. Verification will be done well with the tools and methods that are available.
  • It should be written in as simple a way as possible so that everyone can understand it.
  • It must explain what happened to make an answer necessary.
  • It should be testable in a fair amount of time.
  • It shouldn’t say different things.

Sources Of Hypothesis

Sources of hypothesis are:

  • Patterns of similarity between the phenomenon under investigation and existing hypotheses.
  • Insights derived from prior research, concurrent observations, and insights from opposing perspectives.
  • The formulations are derived from accepted scientific theories and proposed by researchers.
  • In research, it’s essential to consider hypothesis as different subject areas may require various hypotheses (plural form of hypothesis). Researchers also establish a significance level to determine the strength of evidence supporting a hypothesis.
  • Individual cognitive processes also contribute to the formation of hypotheses.

One hypothesis is a tentative explanation for an observation or phenomenon. It is based on prior knowledge and understanding of the world, and it can be tested by gathering and analyzing data. Observed facts are the data that are collected to test a hypothesis. They can support or refute the hypothesis.

For example, the hypothesis that “eating more fruits and vegetables will improve your health” can be tested by gathering data on the health of people who eat different amounts of fruits and vegetables. If the people who eat more fruits and vegetables are healthier than those who eat less fruits and vegetables, then the hypothesis is supported.

Hypotheses are essential for scientific inquiry. They help scientists to focus their research, to design experiments, and to interpret their results. They are also essential for the development of scientific theories.

Types Of Hypothesis

In research, you typically encounter two types of hypothesis: the alternative hypothesis (which proposes a relationship between variables) and the null hypothesis (which suggests no relationship).

Simple Hypothesis

It illustrates the association between one dependent variable and one independent variable. For instance, if you consume more vegetables, you will lose weight more quickly. Here, increasing vegetable consumption is the independent variable, while weight loss is the dependent variable.

Complex Hypothesis

It exhibits the relationship between at least two dependent variables and at least two independent variables. Eating more vegetables and fruits results in weight loss, radiant skin, and a decreased risk of numerous diseases, including heart disease.

Directional Hypothesis

It shows that a researcher wants to reach a certain goal. The way the factors are related can also tell us about their nature. For example, four-year-old children who eat well over a time of five years have a higher IQ than children who don’t eat well. This shows what happened and how it happened.

Non-directional Hypothesis

When there is no theory involved, it is used. It is a statement that there is a connection between two variables, but it doesn’t say what that relationship is or which way it goes.

Null Hypothesis

It says something that goes against the theory. It’s a statement that says something is not true, and there is no link between the independent and dependent factors. “H 0 ” represents the null hypothesis.

Associative and Causal Hypothesis

When a change in one variable causes a change in the other variable, this is called the associative hypothesis . The causal hypothesis, on the other hand, says that there is a cause-and-effect relationship between two or more factors.

Examples Of Hypothesis

Examples of simple hypotheses:

  • Students who consume breakfast before taking a math test will have a better overall performance than students who do not consume breakfast.
  • Students who experience test anxiety before an English examination will get lower scores than students who do not experience test anxiety.
  • Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone, is a statement that suggests that drivers who talk on the phone while driving are more likely to make mistakes.

Examples of a complex hypothesis:

  • Individuals who consume a lot of sugar and don’t get much exercise are at an increased risk of developing depression.
  • Younger people who are routinely exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces, according to a new study.
  • Increased levels of air pollution led to higher rates of respiratory illnesses, which in turn resulted in increased costs for healthcare for the affected communities.

Examples of Directional Hypothesis:

  • The crop yield will go up a lot if the amount of fertilizer is increased.
  • Patients who have surgery and are exposed to more stress will need more time to get better.
  • Increasing the frequency of brand advertising on social media will lead to a significant increase in brand awareness among the target audience.

Examples of Non-Directional Hypothesis (or Two-Tailed Hypothesis):

  • The test scores of two groups of students are very different from each other.
  • There is a link between gender and being happy at work.
  • There is a correlation between the amount of caffeine an individual consumes and the speed with which they react.

Examples of a null hypothesis:

  • Children who receive a new reading intervention will have scores that are different than students who do not receive the intervention.
  • The results of a memory recall test will not reveal any significant gap in performance between children and adults.
  • There is not a significant relationship between the number of hours spent playing video games and academic performance.

Examples of Associative Hypothesis:

  • There is a link between how many hours you spend studying and how well you do in school.
  • Drinking sugary drinks is bad for your health as a whole.
  • There is an association between socioeconomic status and access to quality healthcare services in urban neighborhoods.

Functions Of Hypothesis

The research issue can be understood better with the help of a hypothesis, which is why developing one is crucial. The following are some of the specific roles that a hypothesis plays: (Rashid, Apr 20, 2022)

  • A hypothesis gives a study a point of concentration. It enlightens us as to the specific characteristics of a study subject we need to look into.
  • It instructs us on what data to acquire as well as what data we should not collect, giving the study a focal point .
  • The development of a hypothesis improves objectivity since it enables the establishment of a focal point.
  • A hypothesis makes it possible for us to contribute to the development of the theory. Because of this, we are in a position to definitively determine what is true and what is untrue .

How will Hypothesis help in the Scientific Method?

  • The scientific method begins with observation and inquiry about the natural world when formulating research questions. Researchers can refine their observations and queries into specific, testable research questions with the aid of hypothesis. They provide an investigation with a focused starting point.
  • Hypothesis generate specific predictions regarding the expected outcomes of experiments or observations. These forecasts are founded on the researcher’s current knowledge of the subject. They elucidate what researchers anticipate observing if the hypothesis is true.
  • Hypothesis direct the design of experiments and data collection techniques. Researchers can use them to determine which variables to measure or manipulate, which data to obtain, and how to conduct systematic and controlled research.
  • Following the formulation of a hypothesis and the design of an experiment, researchers collect data through observation, measurement, or experimentation. The collected data is used to verify the hypothesis’s predictions.
  • Hypothesis establish the criteria for evaluating experiment results. The observed data are compared to the predictions generated by the hypothesis. This analysis helps determine whether empirical evidence supports or refutes the hypothesis.
  • The results of experiments or observations are used to derive conclusions regarding the hypothesis. If the data support the predictions, then the hypothesis is supported. If this is not the case, the hypothesis may be revised or rejected, leading to the formulation of new queries and hypothesis.
  • The scientific approach is iterative, resulting in new hypothesis and research issues from previous trials. This cycle of hypothesis generation, testing, and refining drives scientific progress.

Importance Of Hypothesis

  • Hypothesis are testable statements that enable scientists to determine if their predictions are accurate. This assessment is essential to the scientific method, which is based on empirical evidence.
  • Hypothesis serve as the foundation for designing experiments or data collection techniques. They can be used by researchers to develop protocols and procedures that will produce meaningful results.
  • Hypothesis hold scientists accountable for their assertions. They establish expectations for what the research should reveal and enable others to assess the validity of the findings.
  • Hypothesis aid in identifying the most important variables of a study. The variables can then be measured, manipulated, or analyzed to determine their relationships.
  • Hypothesis assist researchers in allocating their resources efficiently. They ensure that time, money, and effort are spent investigating specific concerns, as opposed to exploring random concepts.
  • Testing hypothesis contribute to the scientific body of knowledge. Whether or not a hypothesis is supported, the results contribute to our understanding of a phenomenon.
  • Hypothesis can result in the creation of theories. When supported by substantive evidence, hypothesis can serve as the foundation for larger theoretical frameworks that explain complex phenomena.
  • Beyond scientific research, hypothesis play a role in the solution of problems in a variety of domains. They enable professionals to make educated assumptions about the causes of problems and to devise solutions.

Research Hypotheses: Did you know that a hypothesis refers to an educated guess or prediction about the outcome of a research study?

It’s like a roadmap guiding researchers towards their destination of knowledge. Just like a compass points north, a well-crafted hypothesis points the way to valuable discoveries in the world of science and inquiry.

Choose the best answer. 

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Further reading.

  • RNA-DNA World Hypothesis
  • BYJU’S. (2023). Hypothesis. Retrieved 01 Septermber 2023, from https://byjus.com/physics/hypothesis/#sources-of-hypothesis
  • Collegedunia. (2023). Hypothesis. Retrieved 1 September 2023, from https://collegedunia.com/exams/hypothesis-science-articleid-7026#d
  • Hussain, D. J. (2022). Hypothesis. Retrieved 01 September 2023, from https://mmhapu.ac.in/doc/eContent/Management/JamesHusain/Research%20Hypothesis%20-Meaning,%20Nature%20&%20Importance-Characteristics%20of%20Good%20%20Hypothesis%20Sem2.pdf
  • Media, D. (2023). Hypothesis in the Scientific Method. Retrieved 01 September 2023, from https://www.verywellmind.com/what-is-a-hypothesis-2795239#toc-hypotheses-examples
  • Rashid, M. H. A. (Apr 20, 2022). Research Methodology. Retrieved 01 September 2023, from https://limbd.org/hypothesis-definitions-functions-characteristics-types-errors-the-process-of-testing-a-hypothesis-hypotheses-in-qualitative-research/#:~:text=Functions%20of%20a%20Hypothesis%3A&text=Specifically%2C%20a%20hypothesis%20serves%20the,providing%20focus%20to%20the%20study.

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Last updated on September 8th, 2023

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Verywell / Alex Dos Diaz

  • The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

  • Operationalization

Hypothesis Types

Hypotheses examples.

  • Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

  • Forming a question
  • Performing background research
  • Creating a hypothesis
  • Designing an experiment
  • Collecting data
  • Analyzing the results
  • Drawing conclusions
  • Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

  • Is your hypothesis based on your research on a topic?
  • Can your hypothesis be tested?
  • Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

  • Collect as many observations about a topic or problem as you can.
  • Evaluate these observations and look for possible causes of the problem.
  • Create a list of possible explanations that you might want to explore.
  • After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

  • Does your hypothesis focus on something that you can actually test?
  • Does your hypothesis include both an independent and dependent variable?
  • Can you manipulate the variables?
  • Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

  • Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
  • Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
  • Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
  • Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
  • Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
  • Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

  • "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
  • "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
  • "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
  • "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

  • "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
  • "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

  • "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
  • "There is no difference in scores on a memory recall task between children and adults."
  • "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

  • "People who take St. John's wort supplements will have less anxiety than those who do not."
  • "Adults will perform better on a memory task than children."
  • "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Educational resources and simple solutions for your research journey

Research hypothesis: What it is, how to write it, types, and examples

What is a Research Hypothesis: How to Write it, Types, and Examples

how to make a hypothesis in biology

Any research begins with a research question and a research hypothesis . A research question alone may not suffice to design the experiment(s) needed to answer it. A hypothesis is central to the scientific method. But what is a hypothesis ? A hypothesis is a testable statement that proposes a possible explanation to a phenomenon, and it may include a prediction. Next, you may ask what is a research hypothesis ? Simply put, a research hypothesis is a prediction or educated guess about the relationship between the variables that you want to investigate.  

It is important to be thorough when developing your research hypothesis. Shortcomings in the framing of a hypothesis can affect the study design and the results. A better understanding of the research hypothesis definition and characteristics of a good hypothesis will make it easier for you to develop your own hypothesis for your research. Let’s dive in to know more about the types of research hypothesis , how to write a research hypothesis , and some research hypothesis examples .  

Table of Contents

What is a hypothesis ?  

A hypothesis is based on the existing body of knowledge in a study area. Framed before the data are collected, a hypothesis states the tentative relationship between independent and dependent variables, along with a prediction of the outcome.  

What is a research hypothesis ?  

Young researchers starting out their journey are usually brimming with questions like “ What is a hypothesis ?” “ What is a research hypothesis ?” “How can I write a good research hypothesis ?”   

A research hypothesis is a statement that proposes a possible explanation for an observable phenomenon or pattern. It guides the direction of a study and predicts the outcome of the investigation. A research hypothesis is testable, i.e., it can be supported or disproven through experimentation or observation.     

how to make a hypothesis in biology

Characteristics of a good hypothesis  

Here are the characteristics of a good hypothesis :  

  • Clearly formulated and free of language errors and ambiguity  
  • Concise and not unnecessarily verbose  
  • Has clearly defined variables  
  • Testable and stated in a way that allows for it to be disproven  
  • Can be tested using a research design that is feasible, ethical, and practical   
  • Specific and relevant to the research problem  
  • Rooted in a thorough literature search  
  • Can generate new knowledge or understanding.  

How to create an effective research hypothesis  

A study begins with the formulation of a research question. A researcher then performs background research. This background information forms the basis for building a good research hypothesis . The researcher then performs experiments, collects, and analyzes the data, interprets the findings, and ultimately, determines if the findings support or negate the original hypothesis.  

Let’s look at each step for creating an effective, testable, and good research hypothesis :  

  • Identify a research problem or question: Start by identifying a specific research problem.   
  • Review the literature: Conduct an in-depth review of the existing literature related to the research problem to grasp the current knowledge and gaps in the field.   
  • Formulate a clear and testable hypothesis : Based on the research question, use existing knowledge to form a clear and testable hypothesis . The hypothesis should state a predicted relationship between two or more variables that can be measured and manipulated. Improve the original draft till it is clear and meaningful.  
  • State the null hypothesis: The null hypothesis is a statement that there is no relationship between the variables you are studying.   
  • Define the population and sample: Clearly define the population you are studying and the sample you will be using for your research.  
  • Select appropriate methods for testing the hypothesis: Select appropriate research methods, such as experiments, surveys, or observational studies, which will allow you to test your research hypothesis .  

Remember that creating a research hypothesis is an iterative process, i.e., you might have to revise it based on the data you collect. You may need to test and reject several hypotheses before answering the research problem.  

How to write a research hypothesis  

When you start writing a research hypothesis , you use an “if–then” statement format, which states the predicted relationship between two or more variables. Clearly identify the independent variables (the variables being changed) and the dependent variables (the variables being measured), as well as the population you are studying. Review and revise your hypothesis as needed.  

An example of a research hypothesis in this format is as follows:  

“ If [athletes] follow [cold water showers daily], then their [endurance] increases.”  

Population: athletes  

Independent variable: daily cold water showers  

Dependent variable: endurance  

You may have understood the characteristics of a good hypothesis . But note that a research hypothesis is not always confirmed; a researcher should be prepared to accept or reject the hypothesis based on the study findings.  

how to make a hypothesis in biology

Research hypothesis checklist  

Following from above, here is a 10-point checklist for a good research hypothesis :  

  • Testable: A research hypothesis should be able to be tested via experimentation or observation.  
  • Specific: A research hypothesis should clearly state the relationship between the variables being studied.  
  • Based on prior research: A research hypothesis should be based on existing knowledge and previous research in the field.  
  • Falsifiable: A research hypothesis should be able to be disproven through testing.  
  • Clear and concise: A research hypothesis should be stated in a clear and concise manner.  
  • Logical: A research hypothesis should be logical and consistent with current understanding of the subject.  
  • Relevant: A research hypothesis should be relevant to the research question and objectives.  
  • Feasible: A research hypothesis should be feasible to test within the scope of the study.  
  • Reflects the population: A research hypothesis should consider the population or sample being studied.  
  • Uncomplicated: A good research hypothesis is written in a way that is easy for the target audience to understand.  

By following this research hypothesis checklist , you will be able to create a research hypothesis that is strong, well-constructed, and more likely to yield meaningful results.  

Research hypothesis: What it is, how to write it, types, and examples

Types of research hypothesis  

Different types of research hypothesis are used in scientific research:  

1. Null hypothesis:

A null hypothesis states that there is no change in the dependent variable due to changes to the independent variable. This means that the results are due to chance and are not significant. A null hypothesis is denoted as H0 and is stated as the opposite of what the alternative hypothesis states.   

Example: “ The newly identified virus is not zoonotic .”  

2. Alternative hypothesis:

This states that there is a significant difference or relationship between the variables being studied. It is denoted as H1 or Ha and is usually accepted or rejected in favor of the null hypothesis.  

Example: “ The newly identified virus is zoonotic .”  

3. Directional hypothesis :

This specifies the direction of the relationship or difference between variables; therefore, it tends to use terms like increase, decrease, positive, negative, more, or less.   

Example: “ The inclusion of intervention X decreases infant mortality compared to the original treatment .”   

4. Non-directional hypothesis:

While it does not predict the exact direction or nature of the relationship between the two variables, a non-directional hypothesis states the existence of a relationship or difference between variables but not the direction, nature, or magnitude of the relationship. A non-directional hypothesis may be used when there is no underlying theory or when findings contradict previous research.  

Example, “ Cats and dogs differ in the amount of affection they express .”  

5. Simple hypothesis :

A simple hypothesis only predicts the relationship between one independent and another independent variable.  

Example: “ Applying sunscreen every day slows skin aging .”  

6 . Complex hypothesis :

A complex hypothesis states the relationship or difference between two or more independent and dependent variables.   

Example: “ Applying sunscreen every day slows skin aging, reduces sun burn, and reduces the chances of skin cancer .” (Here, the three dependent variables are slowing skin aging, reducing sun burn, and reducing the chances of skin cancer.)  

7. Associative hypothesis:  

An associative hypothesis states that a change in one variable results in the change of the other variable. The associative hypothesis defines interdependency between variables.  

Example: “ There is a positive association between physical activity levels and overall health .”  

8 . Causal hypothesis:

A causal hypothesis proposes a cause-and-effect interaction between variables.  

Example: “ Long-term alcohol use causes liver damage .”  

Note that some of the types of research hypothesis mentioned above might overlap. The types of hypothesis chosen will depend on the research question and the objective of the study.  

how to make a hypothesis in biology

Research hypothesis examples  

Here are some good research hypothesis examples :  

“The use of a specific type of therapy will lead to a reduction in symptoms of depression in individuals with a history of major depressive disorder.”  

“Providing educational interventions on healthy eating habits will result in weight loss in overweight individuals.”  

“Plants that are exposed to certain types of music will grow taller than those that are not exposed to music.”  

“The use of the plant growth regulator X will lead to an increase in the number of flowers produced by plants.”  

Characteristics that make a research hypothesis weak are unclear variables, unoriginality, being too general or too vague, and being untestable. A weak hypothesis leads to weak research and improper methods.   

Some bad research hypothesis examples (and the reasons why they are “bad”) are as follows:  

“This study will show that treatment X is better than any other treatment . ” (This statement is not testable, too broad, and does not consider other treatments that may be effective.)  

“This study will prove that this type of therapy is effective for all mental disorders . ” (This statement is too broad and not testable as mental disorders are complex and different disorders may respond differently to different types of therapy.)  

“Plants can communicate with each other through telepathy . ” (This statement is not testable and lacks a scientific basis.)  

Importance of testable hypothesis  

If a research hypothesis is not testable, the results will not prove or disprove anything meaningful. The conclusions will be vague at best. A testable hypothesis helps a researcher focus on the study outcome and understand the implication of the question and the different variables involved. A testable hypothesis helps a researcher make precise predictions based on prior research.  

To be considered testable, there must be a way to prove that the hypothesis is true or false; further, the results of the hypothesis must be reproducible.  

Research hypothesis: What it is, how to write it, types, and examples

Frequently Asked Questions (FAQs) on research hypothesis  

1. What is the difference between research question and research hypothesis ?  

A research question defines the problem and helps outline the study objective(s). It is an open-ended statement that is exploratory or probing in nature. Therefore, it does not make predictions or assumptions. It helps a researcher identify what information to collect. A research hypothesis , however, is a specific, testable prediction about the relationship between variables. Accordingly, it guides the study design and data analysis approach.

2. When to reject null hypothesis ?

A null hypothesis should be rejected when the evidence from a statistical test shows that it is unlikely to be true. This happens when the test statistic (e.g., p -value) is less than the defined significance level (e.g., 0.05). Rejecting the null hypothesis does not necessarily mean that the alternative hypothesis is true; it simply means that the evidence found is not compatible with the null hypothesis.  

3. How can I be sure my hypothesis is testable?  

A testable hypothesis should be specific and measurable, and it should state a clear relationship between variables that can be tested with data. To ensure that your hypothesis is testable, consider the following:  

  • Clearly define the key variables in your hypothesis. You should be able to measure and manipulate these variables in a way that allows you to test the hypothesis.  
  • The hypothesis should predict a specific outcome or relationship between variables that can be measured or quantified.   
  • You should be able to collect the necessary data within the constraints of your study.  
  • It should be possible for other researchers to replicate your study, using the same methods and variables.   
  • Your hypothesis should be testable by using appropriate statistical analysis techniques, so you can draw conclusions, and make inferences about the population from the sample data.  
  • The hypothesis should be able to be disproven or rejected through the collection of data.  

4. How do I revise my research hypothesis if my data does not support it?  

If your data does not support your research hypothesis , you will need to revise it or develop a new one. You should examine your data carefully and identify any patterns or anomalies, re-examine your research question, and/or revisit your theory to look for any alternative explanations for your results. Based on your review of the data, literature, and theories, modify your research hypothesis to better align it with the results you obtained. Use your revised hypothesis to guide your research design and data collection. It is important to remain objective throughout the process.  

5. I am performing exploratory research. Do I need to formulate a research hypothesis?  

As opposed to “confirmatory” research, where a researcher has some idea about the relationship between the variables under investigation, exploratory research (or hypothesis-generating research) looks into a completely new topic about which limited information is available. Therefore, the researcher will not have any prior hypotheses. In such cases, a researcher will need to develop a post-hoc hypothesis. A post-hoc research hypothesis is generated after these results are known.  

6. How is a research hypothesis different from a research question?

A research question is an inquiry about a specific topic or phenomenon, typically expressed as a question. It seeks to explore and understand a particular aspect of the research subject. In contrast, a research hypothesis is a specific statement or prediction that suggests an expected relationship between variables. It is formulated based on existing knowledge or theories and guides the research design and data analysis.

7. Can a research hypothesis change during the research process?

Yes, research hypotheses can change during the research process. As researchers collect and analyze data, new insights and information may emerge that require modification or refinement of the initial hypotheses. This can be due to unexpected findings, limitations in the original hypotheses, or the need to explore additional dimensions of the research topic. Flexibility is crucial in research, allowing for adaptation and adjustment of hypotheses to align with the evolving understanding of the subject matter.

8. How many hypotheses should be included in a research study?

The number of research hypotheses in a research study varies depending on the nature and scope of the research. It is not necessary to have multiple hypotheses in every study. Some studies may have only one primary hypothesis, while others may have several related hypotheses. The number of hypotheses should be determined based on the research objectives, research questions, and the complexity of the research topic. It is important to ensure that the hypotheses are focused, testable, and directly related to the research aims.

9. Can research hypotheses be used in qualitative research?

Yes, research hypotheses can be used in qualitative research, although they are more commonly associated with quantitative research. In qualitative research, hypotheses may be formulated as tentative or exploratory statements that guide the investigation. Instead of testing hypotheses through statistical analysis, qualitative researchers may use the hypotheses to guide data collection and analysis, seeking to uncover patterns, themes, or relationships within the qualitative data. The emphasis in qualitative research is often on generating insights and understanding rather than confirming or rejecting specific research hypotheses through statistical testing.

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Original Research: Creating a Hypothesis

  • Initial Steps

Creating a Hypothesis

  • Research Designs and Methods
  • Submitting a Research Plan for Review
  • Performing the Research
  • Analyzing the Data
  • Writing the Research Paper

how to make a hypothesis in biology

After following the initial steps, the researcher should be able to create a hypothesis that can be tested. A hypothesis is a proposed statement that is intended to explain a theory for why something happens. To create a solid hypothesis, make sure it is not listed as a question, but as a prediction statement. To create a research hypothesis there has to be both a dependent and independent variable, and an expected outcome. Independent variables are what may be changed in the experiment to create an outcome. The dependent variable is what the experiment is intended to measure based on changes made to the independent variable. Defining the expected outcome creates the predictive component of the hypothesis that can be tested. Incorporating these elements into a simple predictive statement ensures that you can determine an outcome from the experiment. Ensure that any variables are taken into consideration, and that the results from the hypothesis are measurable.

Types of Hypotheses

There are many types of hypotheses, but the seven most common are the following:

  • Simple Hypothesis - Questions the relationship between the dependent and independent variables.
  • Complex Hypothesis - Questions the effect of multiple dependent and independent variables.
  • Empirical Hypothesis - Often called a working hypothesis, this question is applied to a specific field when looking for empirical evidence.
  • Null Hypothesis - This is used to contradict the expected effect of dependent and independent variables. 
  • Alternative Hypothesis - Several hypotheses are given, but as the experiment proceeds, the alternative hypothesis is introduced to reflect the conditions of the experiment. 
  • Logical Hypothesis - These hypotheses are able to be verified using logic.
  • Statistical Hypothesis ​ - A hypothesis of this type is one that can be proven using statistical analysis.

For more information about how to create a hypothesis, have a look at the  Fundamentals of Research Methodology  by Engwa Godwill. 

Based on the hypothesis created, the researcher will need to determine the best research design for the experiment. 

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How to Write a Research Hypothesis: Good & Bad Examples

how to make a hypothesis in biology

What is a research hypothesis?

A research hypothesis is an attempt at explaining a phenomenon or the relationships between phenomena/variables in the real world. Hypotheses are sometimes called “educated guesses”, but they are in fact (or let’s say they should be) based on previous observations, existing theories, scientific evidence, and logic. A research hypothesis is also not a prediction—rather, predictions are ( should be) based on clearly formulated hypotheses. For example, “We tested the hypothesis that KLF2 knockout mice would show deficiencies in heart development” is an assumption or prediction, not a hypothesis. 

The research hypothesis at the basis of this prediction is “the product of the KLF2 gene is involved in the development of the cardiovascular system in mice”—and this hypothesis is probably (hopefully) based on a clear observation, such as that mice with low levels of Kruppel-like factor 2 (which KLF2 codes for) seem to have heart problems. From this hypothesis, you can derive the idea that a mouse in which this particular gene does not function cannot develop a normal cardiovascular system, and then make the prediction that we started with. 

What is the difference between a hypothesis and a prediction?

You might think that these are very subtle differences, and you will certainly come across many publications that do not contain an actual hypothesis or do not make these distinctions correctly. But considering that the formulation and testing of hypotheses is an integral part of the scientific method, it is good to be aware of the concepts underlying this approach. The two hallmarks of a scientific hypothesis are falsifiability (an evaluation standard that was introduced by the philosopher of science Karl Popper in 1934) and testability —if you cannot use experiments or data to decide whether an idea is true or false, then it is not a hypothesis (or at least a very bad one).

So, in a nutshell, you (1) look at existing evidence/theories, (2) come up with a hypothesis, (3) make a prediction that allows you to (4) design an experiment or data analysis to test it, and (5) come to a conclusion. Of course, not all studies have hypotheses (there is also exploratory or hypothesis-generating research), and you do not necessarily have to state your hypothesis as such in your paper. 

But for the sake of understanding the principles of the scientific method, let’s first take a closer look at the different types of hypotheses that research articles refer to and then give you a step-by-step guide for how to formulate a strong hypothesis for your own paper.

Types of Research Hypotheses

Hypotheses can be simple , which means they describe the relationship between one single independent variable (the one you observe variations in or plan to manipulate) and one single dependent variable (the one you expect to be affected by the variations/manipulation). If there are more variables on either side, you are dealing with a complex hypothesis. You can also distinguish hypotheses according to the kind of relationship between the variables you are interested in (e.g., causal or associative ). But apart from these variations, we are usually interested in what is called the “alternative hypothesis” and, in contrast to that, the “null hypothesis”. If you think these two should be listed the other way round, then you are right, logically speaking—the alternative should surely come second. However, since this is the hypothesis we (as researchers) are usually interested in, let’s start from there.

Alternative Hypothesis

If you predict a relationship between two variables in your study, then the research hypothesis that you formulate to describe that relationship is your alternative hypothesis (usually H1 in statistical terms). The goal of your hypothesis testing is thus to demonstrate that there is sufficient evidence that supports the alternative hypothesis, rather than evidence for the possibility that there is no such relationship. The alternative hypothesis is usually the research hypothesis of a study and is based on the literature, previous observations, and widely known theories. 

Null Hypothesis

The hypothesis that describes the other possible outcome, that is, that your variables are not related, is the null hypothesis ( H0 ). Based on your findings, you choose between the two hypotheses—usually that means that if your prediction was correct, you reject the null hypothesis and accept the alternative. Make sure, however, that you are not getting lost at this step of the thinking process: If your prediction is that there will be no difference or change, then you are trying to find support for the null hypothesis and reject H1. 

Directional Hypothesis

While the null hypothesis is obviously “static”, the alternative hypothesis can specify a direction for the observed relationship between variables—for example, that mice with higher expression levels of a certain protein are more active than those with lower levels. This is then called a one-tailed hypothesis. 

Another example for a directional one-tailed alternative hypothesis would be that 

H1: Attending private classes before important exams has a positive effect on performance. 

Your null hypothesis would then be that

H0: Attending private classes before important exams has no/a negative effect on performance.

Nondirectional Hypothesis

A nondirectional hypothesis does not specify the direction of the potentially observed effect, only that there is a relationship between the studied variables—this is called a two-tailed hypothesis. For instance, if you are studying a new drug that has shown some effects on pathways involved in a certain condition (e.g., anxiety) in vitro in the lab, but you can’t say for sure whether it will have the same effects in an animal model or maybe induce other/side effects that you can’t predict and potentially increase anxiety levels instead, you could state the two hypotheses like this:

H1: The only lab-tested drug (somehow) affects anxiety levels in an anxiety mouse model.

You then test this nondirectional alternative hypothesis against the null hypothesis:

H0: The only lab-tested drug has no effect on anxiety levels in an anxiety mouse model.

hypothesis in a research paper

How to Write a Hypothesis for a Research Paper

Now that we understand the important distinctions between different kinds of research hypotheses, let’s look at a simple process of how to write a hypothesis.

Writing a Hypothesis Step:1

Ask a question, based on earlier research. Research always starts with a question, but one that takes into account what is already known about a topic or phenomenon. For example, if you are interested in whether people who have pets are happier than those who don’t, do a literature search and find out what has already been demonstrated. You will probably realize that yes, there is quite a bit of research that shows a relationship between happiness and owning a pet—and even studies that show that owning a dog is more beneficial than owning a cat ! Let’s say you are so intrigued by this finding that you wonder: 

What is it that makes dog owners even happier than cat owners? 

Let’s move on to Step 2 and find an answer to that question.

Writing a Hypothesis Step 2:

Formulate a strong hypothesis by answering your own question. Again, you don’t want to make things up, take unicorns into account, or repeat/ignore what has already been done. Looking at the dog-vs-cat papers your literature search returned, you see that most studies are based on self-report questionnaires on personality traits, mental health, and life satisfaction. What you don’t find is any data on actual (mental or physical) health measures, and no experiments. You therefore decide to make a bold claim come up with the carefully thought-through hypothesis that it’s maybe the lifestyle of the dog owners, which includes walking their dog several times per day, engaging in fun and healthy activities such as agility competitions, and taking them on trips, that gives them that extra boost in happiness. You could therefore answer your question in the following way:

Dog owners are happier than cat owners because of the dog-related activities they engage in.

Now you have to verify that your hypothesis fulfills the two requirements we introduced at the beginning of this resource article: falsifiability and testability . If it can’t be wrong and can’t be tested, it’s not a hypothesis. We are lucky, however, because yes, we can test whether owning a dog but not engaging in any of those activities leads to lower levels of happiness or well-being than owning a dog and playing and running around with them or taking them on trips.  

Writing a Hypothesis Step 3:

Make your predictions and define your variables. We have verified that we can test our hypothesis, but now we have to define all the relevant variables, design our experiment or data analysis, and make precise predictions. You could, for example, decide to study dog owners (not surprising at this point), let them fill in questionnaires about their lifestyle as well as their life satisfaction (as other studies did), and then compare two groups of active and inactive dog owners. Alternatively, if you want to go beyond the data that earlier studies produced and analyzed and directly manipulate the activity level of your dog owners to study the effect of that manipulation, you could invite them to your lab, select groups of participants with similar lifestyles, make them change their lifestyle (e.g., couch potato dog owners start agility classes, very active ones have to refrain from any fun activities for a certain period of time) and assess their happiness levels before and after the intervention. In both cases, your independent variable would be “ level of engagement in fun activities with dog” and your dependent variable would be happiness or well-being . 

Examples of a Good and Bad Hypothesis

Let’s look at a few examples of good and bad hypotheses to get you started.

Good Hypothesis Examples

Working from home improves job satisfaction.Employees who are allowed to work from home are less likely to quit within 2 years than those who need to come to the office.
Sleep deprivation affects cognition.Students who sleep <5 hours/night don’t perform as well on exams as those who sleep >7 hours/night. 
Animals adapt to their environment.Birds of the same species living on different islands have differently shaped beaks depending on the available food source.
Social media use causes anxiety.Do teenagers who refrain from using social media for 4 weeks show improvements in anxiety symptoms?

Bad Hypothesis Examples

Garlic repels vampires.Participants who eat garlic daily will not be harmed by vampires.Nobody gets harmed by vampires— .
Chocolate is better than vanilla.           No clearly defined variables— .

Tips for Writing a Research Hypothesis

If you understood the distinction between a hypothesis and a prediction we made at the beginning of this article, then you will have no problem formulating your hypotheses and predictions correctly. To refresh your memory: We have to (1) look at existing evidence, (2) come up with a hypothesis, (3) make a prediction, and (4) design an experiment. For example, you could summarize your dog/happiness study like this:

(1) While research suggests that dog owners are happier than cat owners, there are no reports on what factors drive this difference. (2) We hypothesized that it is the fun activities that many dog owners (but very few cat owners) engage in with their pets that increases their happiness levels. (3) We thus predicted that preventing very active dog owners from engaging in such activities for some time and making very inactive dog owners take up such activities would lead to an increase and decrease in their overall self-ratings of happiness, respectively. (4) To test this, we invited dog owners into our lab, assessed their mental and emotional well-being through questionnaires, and then assigned them to an “active” and an “inactive” group, depending on… 

Note that you use “we hypothesize” only for your hypothesis, not for your experimental prediction, and “would” or “if – then” only for your prediction, not your hypothesis. A hypothesis that states that something “would” affect something else sounds as if you don’t have enough confidence to make a clear statement—in which case you can’t expect your readers to believe in your research either. Write in the present tense, don’t use modal verbs that express varying degrees of certainty (such as may, might, or could ), and remember that you are not drawing a conclusion while trying not to exaggerate but making a clear statement that you then, in a way, try to disprove . And if that happens, that is not something to fear but an important part of the scientific process.

Similarly, don’t use “we hypothesize” when you explain the implications of your research or make predictions in the conclusion section of your manuscript, since these are clearly not hypotheses in the true sense of the word. As we said earlier, you will find that many authors of academic articles do not seem to care too much about these rather subtle distinctions, but thinking very clearly about your own research will not only help you write better but also ensure that even that infamous Reviewer 2 will find fewer reasons to nitpick about your manuscript. 

Perfect Your Manuscript With Professional Editing

Now that you know how to write a strong research hypothesis for your research paper, you might be interested in our free AI Proofreader , Wordvice AI, which finds and fixes errors in grammar, punctuation, and word choice in academic texts. Or if you are interested in human proofreading , check out our English editing services , including research paper editing and manuscript editing .

On the Wordvice academic resources website , you can also find many more articles and other resources that can help you with writing the other parts of your research paper , with making a research paper outline before you put everything together, or with writing an effective cover letter once you are ready to submit.

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How to Develop a Good Research Hypothesis

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The story of a research study begins by asking a question. Researchers all around the globe are asking curious questions and formulating research hypothesis. However, whether the research study provides an effective conclusion depends on how well one develops a good research hypothesis. Research hypothesis examples could help researchers get an idea as to how to write a good research hypothesis.

This blog will help you understand what is a research hypothesis, its characteristics and, how to formulate a research hypothesis

Table of Contents

What is Hypothesis?

Hypothesis is an assumption or an idea proposed for the sake of argument so that it can be tested. It is a precise, testable statement of what the researchers predict will be outcome of the study.  Hypothesis usually involves proposing a relationship between two variables: the independent variable (what the researchers change) and the dependent variable (what the research measures).

What is a Research Hypothesis?

Research hypothesis is a statement that introduces a research question and proposes an expected result. It is an integral part of the scientific method that forms the basis of scientific experiments. Therefore, you need to be careful and thorough when building your research hypothesis. A minor flaw in the construction of your hypothesis could have an adverse effect on your experiment. In research, there is a convention that the hypothesis is written in two forms, the null hypothesis, and the alternative hypothesis (called the experimental hypothesis when the method of investigation is an experiment).

Characteristics of a Good Research Hypothesis

As the hypothesis is specific, there is a testable prediction about what you expect to happen in a study. You may consider drawing hypothesis from previously published research based on the theory.

A good research hypothesis involves more effort than just a guess. In particular, your hypothesis may begin with a question that could be further explored through background research.

To help you formulate a promising research hypothesis, you should ask yourself the following questions:

  • Is the language clear and focused?
  • What is the relationship between your hypothesis and your research topic?
  • Is your hypothesis testable? If yes, then how?
  • What are the possible explanations that you might want to explore?
  • Does your hypothesis include both an independent and dependent variable?
  • Can you manipulate your variables without hampering the ethical standards?
  • Does your research predict the relationship and outcome?
  • Is your research simple and concise (avoids wordiness)?
  • Is it clear with no ambiguity or assumptions about the readers’ knowledge
  • Is your research observable and testable results?
  • Is it relevant and specific to the research question or problem?

research hypothesis example

The questions listed above can be used as a checklist to make sure your hypothesis is based on a solid foundation. Furthermore, it can help you identify weaknesses in your hypothesis and revise it if necessary.

Source: Educational Hub

How to formulate a research hypothesis.

A testable hypothesis is not a simple statement. It is rather an intricate statement that needs to offer a clear introduction to a scientific experiment, its intentions, and the possible outcomes. However, there are some important things to consider when building a compelling hypothesis.

1. State the problem that you are trying to solve.

Make sure that the hypothesis clearly defines the topic and the focus of the experiment.

2. Try to write the hypothesis as an if-then statement.

Follow this template: If a specific action is taken, then a certain outcome is expected.

3. Define the variables

Independent variables are the ones that are manipulated, controlled, or changed. Independent variables are isolated from other factors of the study.

Dependent variables , as the name suggests are dependent on other factors of the study. They are influenced by the change in independent variable.

4. Scrutinize the hypothesis

Evaluate assumptions, predictions, and evidence rigorously to refine your understanding.

Types of Research Hypothesis

The types of research hypothesis are stated below:

1. Simple Hypothesis

It predicts the relationship between a single dependent variable and a single independent variable.

2. Complex Hypothesis

It predicts the relationship between two or more independent and dependent variables.

3. Directional Hypothesis

It specifies the expected direction to be followed to determine the relationship between variables and is derived from theory. Furthermore, it implies the researcher’s intellectual commitment to a particular outcome.

4. Non-directional Hypothesis

It does not predict the exact direction or nature of the relationship between the two variables. The non-directional hypothesis is used when there is no theory involved or when findings contradict previous research.

5. Associative and Causal Hypothesis

The associative hypothesis defines interdependency between variables. A change in one variable results in the change of the other variable. On the other hand, the causal hypothesis proposes an effect on the dependent due to manipulation of the independent variable.

6. Null Hypothesis

Null hypothesis states a negative statement to support the researcher’s findings that there is no relationship between two variables. There will be no changes in the dependent variable due the manipulation of the independent variable. Furthermore, it states results are due to chance and are not significant in terms of supporting the idea being investigated.

7. Alternative Hypothesis

It states that there is a relationship between the two variables of the study and that the results are significant to the research topic. An experimental hypothesis predicts what changes will take place in the dependent variable when the independent variable is manipulated. Also, it states that the results are not due to chance and that they are significant in terms of supporting the theory being investigated.

Research Hypothesis Examples of Independent and Dependent Variables

Research Hypothesis Example 1 The greater number of coal plants in a region (independent variable) increases water pollution (dependent variable). If you change the independent variable (building more coal factories), it will change the dependent variable (amount of water pollution).
Research Hypothesis Example 2 What is the effect of diet or regular soda (independent variable) on blood sugar levels (dependent variable)? If you change the independent variable (the type of soda you consume), it will change the dependent variable (blood sugar levels)

You should not ignore the importance of the above steps. The validity of your experiment and its results rely on a robust testable hypothesis. Developing a strong testable hypothesis has few advantages, it compels us to think intensely and specifically about the outcomes of a study. Consequently, it enables us to understand the implication of the question and the different variables involved in the study. Furthermore, it helps us to make precise predictions based on prior research. Hence, forming a hypothesis would be of great value to the research. Here are some good examples of testable hypotheses.

More importantly, you need to build a robust testable research hypothesis for your scientific experiments. A testable hypothesis is a hypothesis that can be proved or disproved as a result of experimentation.

Importance of a Testable Hypothesis

To devise and perform an experiment using scientific method, you need to make sure that your hypothesis is testable. To be considered testable, some essential criteria must be met:

  • There must be a possibility to prove that the hypothesis is true.
  • There must be a possibility to prove that the hypothesis is false.
  • The results of the hypothesis must be reproducible.

Without these criteria, the hypothesis and the results will be vague. As a result, the experiment will not prove or disprove anything significant.

What are your experiences with building hypotheses for scientific experiments? What challenges did you face? How did you overcome these challenges? Please share your thoughts with us in the comments section.

Frequently Asked Questions

The steps to write a research hypothesis are: 1. Stating the problem: Ensure that the hypothesis defines the research problem 2. Writing a hypothesis as an 'if-then' statement: Include the action and the expected outcome of your study by following a ‘if-then’ structure. 3. Defining the variables: Define the variables as Dependent or Independent based on their dependency to other factors. 4. Scrutinizing the hypothesis: Identify the type of your hypothesis

Hypothesis testing is a statistical tool which is used to make inferences about a population data to draw conclusions for a particular hypothesis.

Hypothesis in statistics is a formal statement about the nature of a population within a structured framework of a statistical model. It is used to test an existing hypothesis by studying a population.

Research hypothesis is a statement that introduces a research question and proposes an expected result. It forms the basis of scientific experiments.

The different types of hypothesis in research are: • Null hypothesis: Null hypothesis is a negative statement to support the researcher’s findings that there is no relationship between two variables. • Alternate hypothesis: Alternate hypothesis predicts the relationship between the two variables of the study. • Directional hypothesis: Directional hypothesis specifies the expected direction to be followed to determine the relationship between variables. • Non-directional hypothesis: Non-directional hypothesis does not predict the exact direction or nature of the relationship between the two variables. • Simple hypothesis: Simple hypothesis predicts the relationship between a single dependent variable and a single independent variable. • Complex hypothesis: Complex hypothesis predicts the relationship between two or more independent and dependent variables. • Associative and casual hypothesis: Associative and casual hypothesis predicts the relationship between two or more independent and dependent variables. • Empirical hypothesis: Empirical hypothesis can be tested via experiments and observation. • Statistical hypothesis: A statistical hypothesis utilizes statistical models to draw conclusions about broader populations.

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Wow! You really simplified your explanation that even dummies would find it easy to comprehend. Thank you so much.

Thanks a lot for your valuable guidance.

I enjoy reading the post. Hypotheses are actually an intrinsic part in a study. It bridges the research question and the methodology of the study.

Useful piece!

This is awesome.Wow.

It very interesting to read the topic, can you guide me any specific example of hypothesis process establish throw the Demand and supply of the specific product in market

Nicely explained

It is really a useful for me Kindly give some examples of hypothesis

It was a well explained content ,can you please give me an example with the null and alternative hypothesis illustrated

clear and concise. thanks.

So Good so Amazing

Good to learn

Thanks a lot for explaining to my level of understanding

Explained well and in simple terms. Quick read! Thank you

It awesome. It has really positioned me in my research project

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how to make a hypothesis in biology

  • Research Paper >

How to Write a Hypothesis

Often, one of the trickiest parts of designing and writing up any research paper is writing the hypothesis.

This article is a part of the guide:

  • Outline Examples
  • Example of a Paper
  • Introduction
  • Example of a Paper 2

Browse Full Outline

  • 1 Write a Research Paper
  • 2 Writing a Paper
  • 3.1 Write an Outline
  • 3.2 Outline Examples
  • 4.1 Thesis Statement
  • 4.2 Write a Hypothesis
  • 5.2 Abstract
  • 5.3 Introduction
  • 5.4 Methods
  • 5.5 Results
  • 5.6 Discussion
  • 5.7 Conclusion
  • 5.8 Bibliography
  • 6.1 Table of Contents
  • 6.2 Acknowledgements
  • 6.3 Appendix
  • 7.1 In Text Citations
  • 7.2 Footnotes
  • 7.3.1 Floating Blocks
  • 7.4 Example of a Paper
  • 7.5 Example of a Paper 2
  • 7.6.1 Citations
  • 7.7.1 Writing Style
  • 7.7.2 Citations
  • 8.1.1 Sham Peer Review
  • 8.1.2 Advantages
  • 8.1.3 Disadvantages
  • 8.2 Publication Bias
  • 8.3.1 Journal Rejection
  • 9.1 Article Writing
  • 9.2 Ideas for Topics

The entire experiment revolves around the research hypothesis (H 1 ) and the null hypothesis (H 0 ), so making a mistake here could ruin the whole design .

Needless to say, it can all be a little intimidating, and many students find this to be the most difficult stage of the scientific method .

In fact, it is not as difficult as it looks, and if you have followed the steps of the scientific process and found an area of research and potential research problem , then you may already have a few ideas.

It is just about making sure that you are asking the right questions and wording your hypothesis statements correctly.

Once you have nailed down a promising hypothesis, the rest of the process will flow a lot more easily.

how to make a hypothesis in biology

The Three-Step Process

It can quite difficult to isolate a testable hypothesis after all of the research and study. The best way is to adopt a three-step hypothesis; this will help you to narrow things down, and is the most foolproof guide to how to write a hypothesis.

Step one is to think of a general hypothesis, including everything that you have observed and reviewed during the information gathering stage of any research design . This stage is often called developing the research problem .

how to make a hypothesis in biology

An Example of How to Write a Hypothesis

A worker on a fish-farm notices that his trout seem to have more fish lice in the summer, when the water levels are low, and wants to find out why. His research leads him to believe that the amount of oxygen is the reason - fish that are oxygen stressed tend to be more susceptible to disease and parasites.

He proposes a general hypothesis.

“Water levels affect the amount of lice suffered by rainbow trout.”

This is a good general hypothesis, but it gives no guide to how to design the research or experiment . The hypothesis must be refined to give a little direction.

“Rainbow trout suffer more lice when water levels are low.”

Now there is some directionality, but the hypothesis is not really testable , so the final stage is to design an experiment around which research can be designed, i.e. a testable hypothesis.

“Rainbow trout suffer more lice in low water conditions because there is less oxygen in the water.”

This is a testable hypothesis - he has established variables , and by measuring the amount of oxygen in the water, eliminating other controlled variables , such as temperature, he can see if there is a correlation against the number of lice on the fish.

This is an example of how a gradual focusing of research helps to define how to write a hypothesis .

The Next Stage - What to Do with the Hypothesis

Once you have your hypothesis , the next stage is to design the experiment , allowing a statistical analysis of data, and allowing you to test your hypothesis .

The statistical analysis will allow you to reject either the null or the alternative hypothesis. If the alternative is rejected, then you need to go back and refine the initial hypothesis or design a completely new research program.

This is part of the scientific process, striving for greater accuracy and developing ever more refined hypotheses.

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Martyn Shuttleworth (Aug 1, 2009). How to Write a Hypothesis. Retrieved Aug 28, 2024 from Explorable.com: https://explorable.com/how-to-write-a-hypothesis

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Mr. Dr. Science Teacher

A high school science teacher with a Ph.D. in Ecology and Evolution reflects on teaching, science, and teaching science in the public schools.

Teaching the Hypothesis

Update: Much of the explanation below I’ve now published in a paper in The American Biology Teacher – Strode, P. K. (2015). Hypothesis Generation in Biology: A Science Teaching Challenge & Potential Solution. The American Biology Teacher 77:500-506 . In the paper I show, by way of eight years of data from the International Science and Engineering Fair, a textbook survey, and a teacher survey, how we are misleading our students by not helping them understand and practice this fundamental component of scientific thinking.

In science, there is no one scientific method . In fact, we practice scientific methodology in various ways. Sometimes we make observations about nature, ask questions, answer those questions with explanations or generalizations (hypotheses), and test our answers with predictions and experiments. However, while sometimes the way we do science is mostly linear, most of the time this approach to science is oversimplified. ‘Understanding Science’ at the University of California at Berkeley provides some excellent details on the nonlinear nature of the scientific process here and here .

Scientific methodologies are also not always hypothesis-driven. Some scientific pursuits are based in discovery science , in other words, looking for relationships in nature or simply measuring and describing things in nature. But discovery science can generate hypotheses. Other scientific methodology involves testing models . For example, we use the semipermeable model to describe the cell membrane. We can test this model by predicting that some kinds of substances can move across the cell membrane and while others cannot. Models can function as testable hypotheses–explanations for how this work or are put together–but that is not the focus of this particular post. Finally, we have engineering, computer science, and mathematics. Engineers, computer scientists, and mathematicians identify problems, formulate goals, and design solutions to the problems.

The most common way a hypothesis is used in scientific research is as a tentative, testable, and falsifiable statement that explains some observed phenomenon in nature. We more specifically call this kind of statement an explanatory hypothesis . However, a hypothesis can also be a statement that describes an observed pattern in nature. In this case we call the statement a generalizing hypothesis . Explanatory hypotheses are often used to explain the mechanisms behind the patterns that generalizing hypotheses describe. The hypothesis statement can be followed by the specific, measurable prediction you can make if the hypothesis is valid. Thus, we can think of the hypothesis in science as an explanation or generalization on trial. 

More on distinguishing the generalizing from the explanatory hypothesis can be found in this more recent post,  Generalizing vs Explanatory Hypotheses: How do we use them in Practice?

A prediction in science is a prophecy, a specific and measurable event that is likely to happen in the future as the result of an experiment if the hypothesis is valid.

This post focuses on science that is driven by explanatory hypotheses and aims to help teachers understand what an explanatory hypothesis is in science and how the hypothesis is different from the prediction .

Teaching the Hypothesis Incorrectly

Many teachers and even many textbooks never mention the generalizing hypothesis and teach the hypothesis in a way that makes it no different from a prediction. They teach students to write “If – then” statements for their hypotheses. This approach results in the incorrect form: If I do X, then Y will happen. There is no hypothesis here. This is simply a method ( if I do X ) followed by a prediction ( then Y will happen ). Some teachers and textbooks add “ …because… ” at the end of the “If…, then…” statement. The because statement is often close to the hypothesis that is being tested, but it still does not carefully delineate the hypothesis from the prediction. Indeed, even professional scientists can make mistakes. This misunderstanding that has been born out of science education is not new. In fact, science education specialist and the current editor of The American Biology teacher, William McComas, identified the hypothesis-as-prediction idea as one of the Ten Myths of Science  nearly 20 years ago.

See Table 1 for some authentic good and bad examples.

Table 1. Authentic examples of incorrectly and correctly written hypotheses from (i) students at the Intel International Science and Engineering Fair, (ii) science textbooks, (iii) teachers, and (iv) scientific papers.

Incorrect Correct
ISEFStudents If a plant receives fertilizer, then they will grow to be bigger than a plant that doesn’t receive fertilizer. It is hypothesized that the structural and functional integrity of the system as a whole is dependent on nerve activity.
I believe resin content of various pine species will affect its energy output. The foraging patterns of , as measured by directional response, are affected by electrical fields.
It is hypothesized that a forefoot strike pattern will correlate with lower ground reaction forces. Aspirin inhibits key oncogenic factors and/or activates pivotal tumor suppressor genes.
Textbooks If food is present in the aquarium, then snails will move with greater speed (toward the food) (Green 2004). Marsh grass growth is limited by available nitrogen (Miller and Levine 2010).
Teachers If the farmer burns the prairie then the next year will produce taller plants in his field then the previous year. The fire is replenishing the nutrients in the soil.
Scientists We aimed to test the hypothesis of whether young healthy women will increase muscle mass and lose fat mass after undergoing 12 wk of intense resistance training (Josse et al. 2010). Based on this observation, we hypothesized that natural selection may have influenced copy number in certain human populations (Perry et al. 2007).

How to Correctly Formulate an Explanatory Hypothesis

Let’s say an ecologist notices that in some spring seasons, the migrating birds arrive at their breeding grounds about the same time that their food resources are peaking in availability. The ecologist may explain this observed pattern with the hypothesis that the birds and their food resources, usually insects, are responding to the same environmental cues (warm spring temperatures). The ecologist could then predict that regardless of how early spring comes, the birds and their insect prey will always be somewhat synchronized in time. The ecologist could then test the hypothesis and prediction by carefully recording numbers of birds and their insect prey over several spring seasons. However, there are alternative hypotheses in this scenario. For example, the birds and insects may be using different environmental cues. Perhaps the birds use day length to time their movements north in the spring and the insects do in fact use temperature. If this hypothesis is valid, then the ecologist can predict that the birds and insects will become unsynchronized in early springs–the insects hatching early with the birds arriving the same time on the calendar as they always do.

Explanatory Hypothesis 1 : Birds and insects respond to the same environmental cues, mostly temperature.

Related Prediction : Birds and insects will remain synchronized regardless of the timing of spring.

Explanatory Hypothesis 2 : Birds use day length while insects respond to spring temperatures.

Related Prediction : In early or late springs, the birds will arrive at the same time as always, while the insects will appear either early or late, depending on the timing of spring.

The Research Hypothesis

Some scientists, in their experimental planning stages, write a large, sophisticated statement called the research hypothesis . Research hypotheses force scientists to be very clear to themselves and their colleagues about exactly what the explanation is that they are testing, the general method they are using to test it, and the prediction they can make if their explanation is reasonable. The research hypothesis follows the form: If X is a valid hypothesis (explanation), and I perform Y methods (experiment), then I can predict Z as a specific measurable outcome .

In the birds and their insect prey in spring example, the ecologist may state her research hypothesis the following way:

If birds use day length to time their movements north in the spring while their insect prey respond to warming spring temperatures, and I record the daily number of birds and insects in a forest for several spring seasons in a row, then in early springs the birds will arrive after the insects have already peaked in abundance while in late springs the birds will arrive before there are enough insects for them to eat.

While the above example is a sophisticated way to state a hypothesis, it may not be the best way to communicate a hypothesis and prediction in a scientific report or in a science fair setting.

Getting Students to Think About Hypotheses: Imagine this scenario…

A teacher was guiding her students through science inquiry. She had her students analyze several small tomato plants she had grown and record their observations. The students observed that roughly half of the plants were taller, were darker green, and had more leaves than the other plants. The students also noticed that on stickers on the pots with the better-looking plants was the word “nitrogen.” Following is a hypothetical conversation the teacher might have with one of her students to help him plan an experiment and work on his understanding on the role of the hypothesis in scientific methodology. Notice how the teacher reinforces to the student that the hypothesis is an explanation.

Teacher: “What did you observe about the tomato plants?”

Student: “I noticed that the plants labeled nitrogen were taller, were darker green, and had more leaves than the other plants.”

Teacher: “What hypothesis can you think of that might explain what you observed?”

Student: “I think that if I add nitrogen to some tomato plants, then they will grow taller than other plants.”

Teacher: “Okay, what you have given me is a prediction of what you think will happen in an experiment. Why are you able to make that prediction?”

Student: “Because it looks like the nitrogen helps the plants grow better.”

Teacher: “That’s more like it! What you’ve just given me is an explanation, an actual hypothesis. But I think you can make it even better. For example, w e call those materials that organisms take in from the environment and are helpful in growth and development nutrients. Knowing that, how can you improve that hypothesis?”

Student: “How about nitrogen is a nutrient and helps plants like tomatoes grow better.”

Teacher: “Sounds great! Now you’ve got a testable explanatory hypothesis. How could you design an experiment to test this hypothesis?”

Student: “I could grow tomato plants in ten separate pots of soil with no fertilizer and other tomato plants in ten separate pots of soil with nitrogen fertilizer.”

Teacher: “So, the only variable you would change is presence or absence of fertilizer and you would keep all other variables the same?”

Student: “Yes.”

Teacher: “What then is your prediction if your nitrogen nutrient hypothesis is a valid explanation? In other words, what outcome of your experiment can you measure that will provide support for your hypothesis?”

Student: “I think the tomato plants grown with nitrogen fertilizer will grow faster and maybe even taller than the tomato plants without fertilizer.”

The teacher then had students write their hypotheses and predictions in their lab notebooks as separate statements:

Explanatory Hypothesis : Plants need nitrogen as a nutrient for growth.

Prediction : Tomato plants with grown nitrogen fertilizer will grow faster and taller and have more leaves than tomato plants grown without nitrogen fertilizer.

In summary , here are three things to think about when you are guiding your students in science inquiry:

  • Have students think carefully about their projects and help them decide if a hypothesis is appropriate. They may in fact be doing discovery science, be testing a model, or pursuing an engineering goal. In these cases, asking students to write hypotheses results in students writing predictions, or just being really confused.
  • If the project is testing a hypothesis, help students avoid “If – then” statements because it becomes too likely they will write a method followed by a prediction and forget the hypothesis altogether. If a student does write a prediction and call it a hypothesis, ask them why they are able to make that particular prediction and emphasize that hypotheses are explanations or generalizations about nature .
  • Instruct students to carefully separate their hypotheses from their predictions in their planning so that they are able to articulate, “This is the overarching explanation or generalization I am testing and this is the prediction I can make if my hypothesis is valid.”

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35 Comments

The tone of this article makes it impossible for me to read it.

Hi Kim: I would love a little more detail about the ‘tone’ so I can improve communicating this issue to the science education community. I also encourage you to keep an eye out for a paper of mine on this topic that The American Biology Teacher will be publishing next month. In the journal article, in addition to presenting data on how pervasive the misunderstanding is and how to fix it, I talk about how I struggled with teaching the hypothesis for my first eight years of teaching science. It wasn’t until a graduate school committee member confronted me about my misunderstanding (and encouraged me to fix it) that I was really forced to rethink the concept. It was an incredibly humbling and difficult moment for me. I talk about more of these moment of mine in a later post on the impostor syndrome.

Unless the word “hypothesis” is stated or understood as such – most of your examples which are deemed the correct way of stating a hypothesis sounds as if you were stating a fact – which a hypothesis is not.

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I agree that hypothesis statements can sound like statements of fact, but what makes the statements hypotheses is that they are testable and falsifiable. For example, an evolutionary geneticist may hypothesize that the number of AMY-1 genes (the gene that codes for the saliva protein alpha amylase) a person has is related to the diet of the culture from which he or she has descended. So, the assumption being tested is: AMY-1 gene copy number is related to a person’s culture’s diet. That’s a falsifiable (thus tentative) and testable statement–it’s not a fact specifically. A fact is an actual measured occurrence. For example, the statement ‘species have evolved over time’ is an actual measured occurrence. Ideas for how a particular species evolved are hypotheses.

Excellent and relevant article. And very well written and understandable…something I can use in my science classroom to help students understand hypotheses and how science is done. Thank you for sharing your experiences and knowledge!

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I agree with Donna – this will help with challenging higher end 6th graders and those who are ready by the end of the year to push their hypothesis writing. Some may not quite be ready for this level of thought and stay on the “predication” hypothesis style longer as it is any easy statement.

Thanks for the comment, Alice, and Donna earlier. If you end up having younger students write predictions in place of hypotheses, make sure that they know they are writing predictions and not hypotheses. But I do think young science students can be encouraged to try to verbalized hypotheses by being ask why they are making their specific predictions. From my upcoming American Biology Teacher paper: “Indeed, it is human nature to formulate explanations for observed natural phenomena (Brewer et al., 1998; Lawson, 2004). Cognitive scientists sometimes argue that children are themselves “little scientists.” For example, children with little or no formal training in the process of science can propose func- tional hypotheses to explain a natural event (Vosniadou & Brewer, 1992) and causal hypotheses to explain how one event in nature may affect another (Samarapungavan & Wiers, 1997). Have we, the science educators, excised reasoning skill from our students?”

The formatting of this article makes it impossible to read. Font way too large, huge margins, loads of italics.

Nate: Thanks for this feedback, but I’m confused. the formatting looks fine on my PC and my Mac. What kind of device are you using to read the post?

I’ve tried to clean it up a little, Nate, but I’m at the mercy of WordPress and its defaults–margins, font size, etc.

FINALLY! Thank you for this article! We have been teaching students if…then for so long because it is so difficult to push students into developing a correct hypothesis. The way you model teaching students to develop a proper hypothesis is enlightening and I’m excited to try it; however, I am curious how 8th graders will respond to this way of thinking. Any suggestions on how to make this 8th grader friendly? On a different note, I agree with Nate about the super large font (20+) and italicized print. I’m using an Elitebook laptop and it is difficult to read, although not impossible. Thanks again!

Thanks, Kimberly! Look for my article on this topic in the next issue of The American Biology Teacher. Regarding 8th graders, just like I discuss in this post, I would let them make predictions and have them label them as predictions, then I would ask them WHY they are able to make their predictions. Have them label their answers to that question as their hypothesis and have them write it above the prediction. I am doing this with my high school freshman (I had them read this post!) and they are slowly making the shift from what they learned incorrectly in middle and elementary school.

Great article. What is the title of your paper so I can look for it?

Hypothesis Generation in Biology: A Science Teaching Challenge & Potential Solution

In many ways, your definition of a hypothesis is similar to that of a theory.How do you differentiate between a hypothesis and a theory, or are they the same for you?

Hi John: What is your definition of theory? I follow what the National Academy of Sciences says: a theory is “a well-substantiated explanation of some aspect of the natural world that can incorporate facts, laws, inferences, and tested hypotheses.”

http://www.nap.edu/read/6024/chapter/2#2

So theories are explanatory frameworks for natural phenomena that also have predictive power and that themselves can generate additional hypotheses.

I, too, wonder how theory differs from hypothesis in your presentation. The definitions you quote (hypothesis: “…a tentative, testable, and falsifiable statement that explains some observed phenomenon in nature” and theory: “…a well-substantiated explanation of some aspect of the natural world…”) seem to be gradations of the same thing– the transition from “tentative” to “well-substantiated.” Since the boundary between these terms is subjective, I’m not sure the distinction is meaningful.

My real concern is not the terminology, but rather the focus. If we dwell upon terms we can miss the lesson: all of science is about 3 steps: 1. Observe nature 2. Explain what we see 3. Find out if we’re right

Observations, explanations and experiments are easily grasped by young (or old!) minds, and provide meaningful distinctions in class.

Thanks for your comment, Erik. I think the distinction between the textbook definition of the explanatory hypothesis and the NAS definition of theory is the bit where theories “can incorporate facts, laws, inferences, and tested hypotheses.” At this level, theories, like Evolutionary Theory, become frameworks of understanding from which we can launch more hypotheses. Theories also have far more predictive power than hypotheses (immature theories). I do like your simplified model of scientific thinking! However, given that the term ‘hypothesis’ is so much a part of the language of science, and indeed is how scientists themselves talk and write about their ideas, it’s important that we help students distinguish between testing underlying explanations and patterns (explanatory and generalizing hypotheses) and generating predictions based on those hypotheses. This distinction is what I am trying to help clear up.

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I have a query about the article, where am i able to e-mail the writer?

You can query me here, or personal message me on Facebook or Twitter.

I’m a new science teacher and this confusion between hypothesis and prediction has been a major bugaboo for me for quite a while. I’ve been looking for articles that will back up the case that a hypothesis must be explanatory and does not take the form of “IF… THEN….” Thank you for taking some time to help spread the word!

I’m curious if you recommend any good lesson plans that make this distinction more clearly at the high school level? I’m always looking to beg, borrow, and steal lessons so I can avoid reinventing the wheel, but so many hypothesis lessons out there fall into the exact trap you write about. I even came across a worksheet that said “Make a prediction as to which group will have the fastest car, and therefore the shortest time from A to B. This prediction is your group’s hypothesis.” The rest of the activity is pretty good, but that sentence alone makes the whole lesson plan untenable without major revisions.

Thanks for your comment, Jared! My advise is for you to write your own versions of the activities you like so that you can be sure that your students are being prompted to write actual hypotheses (explanatory or generalizing) followed by predictions as separate and labeled statements. Have you looked at my more recent post on the distinction between generalizing and explanatory hypotheses? When I started approaching hypotheses this way with my students, it created a lot of freedom for them when they were trying to write testable hypotheses. https://mrdrscienceteacher.wordpress.com/2016/08/11/generalizing-vs-explanatory-hypotheses-how-do-we-use-them-in-practice/

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A good article about how, what and why of an hypothesis. An educator from India

Hi Paul, I really appreciate your distinction between the hypothesis, theory, and prediction. I also benefited from additional information found in reading all of the comments regarding your article. I am a college student ready to graduate this fall with my bachelors degree in Human Development with a minor in Anthropology. I will then further my education in obtaining my doctorate in research psychology. I recently experienced challenges in both of my current fields with the structure of my hypotheses. I was at a loss, as I have been working on my bachelors degree for TEN years on and off! I couldn’t figure out why I was having such difficulty at this point in constructing a simple hypothesis to begin my research! But then a professor of mine gave me some advice in an email. With additional confusion and desire to truly understand ALL of my methods to my research before graduation, I scanned the internet in hopes for some elaboration on my professor’s guidance. Your article has truly saved my education!! I now have a much better grasp on verbiage regarding the beginnings to my research. This is important to me as I am VERY particular when it comes to verbiage, meaning, and implications! Thank you so much for your aid in my education! I will be sure to read your article regarding explanatory hypotheses and distinguishing them from the generalized hypothesis! Thank you very much! I wish you the best and hope you continue your path in educating both children and adults! Best regards, Ashley

I really appreciate this feedback, Ashley!

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Hello Dr. Mr. Paul – This article is cited by this page: https://blog.citsci.org/2018/02/28/writing-hypotheses/comment-page-1/?unapproved=751&moderation-hash=a8f05975ec6e41782d07b7886c8bc6ea#comment-751

Have a look. I think you’ll be interested to find that they’re pushing the “if-then” format there. How do we stop this??

Best, – Dr. Mrs. Professor Hennessy

Thank you for letting me know, Dr. Mrs. Professor Hennessy! I’ve sent them an email pointing out the inconsistency in their explanation of how to write good hypotheses.

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COMMENTS

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  3. A Strong Hypothesis

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  7. Step-by-Step Guide: How to Craft a Strong Research Hypothesis

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  9. How to Write a Strong Hypothesis

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    Types of Hypotheses. Simple Hypothesis - Questions the relationship between the dependent and independent variables. Complex Hypothesis - Questions the effect of multiple dependent and independent variables. Empirical Hypothesis - Often called a working hypothesis, this question is applied to a specific field when looking for empirical evidence.

  15. How to Write a Hypothesis w/ Strong Examples

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  16. Writing a Hypothesis for Your Science Fair Project

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    It can quite difficult to isolate a testable hypothesis after all of the research and study. The best way is to adopt a three-step hypothesis; this will help you to narrow things down, and is the most foolproof guide to how to write a hypothesis. Step one is to think of a general hypothesis, including everything that you have observed and ...

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