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What is a Hypothesis – Types, Examples and Writing Guide

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Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

• Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
• Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
• Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
• Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
• Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
• Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

• Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
• Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
• Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
• Education : “Implementing a new teaching method will result in higher student achievement scores.”
• Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
• Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
• Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

• In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
• In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
• I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

• Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
• Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
• Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
• Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
• Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
• Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
• Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

• Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
• Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
• Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
• Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
• Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
• Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

• Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
• May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
• May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
• Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
• Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
• May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

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S.3 hypothesis testing.

In reviewing hypothesis tests, we start first with the general idea. Then, we keep returning to the basic procedures of hypothesis testing, each time adding a little more detail.

The general idea of hypothesis testing involves:

• Making an initial assumption.
• Collecting evidence (data).
• Based on the available evidence (data), deciding whether to reject or not reject the initial assumption.

Every hypothesis test — regardless of the population parameter involved — requires the above three steps.

Example S.3.1

Is normal body temperature really 98.6 degrees f section  .

Consider the population of many, many adults. A researcher hypothesized that the average adult body temperature is lower than the often-advertised 98.6 degrees F. That is, the researcher wants an answer to the question: "Is the average adult body temperature 98.6 degrees? Or is it lower?" To answer his research question, the researcher starts by assuming that the average adult body temperature was 98.6 degrees F.

Then, the researcher went out and tried to find evidence that refutes his initial assumption. In doing so, he selects a random sample of 130 adults. The average body temperature of the 130 sampled adults is 98.25 degrees.

Then, the researcher uses the data he collected to make a decision about his initial assumption. It is either likely or unlikely that the researcher would collect the evidence he did given his initial assumption that the average adult body temperature is 98.6 degrees:

• If it is likely , then the researcher does not reject his initial assumption that the average adult body temperature is 98.6 degrees. There is not enough evidence to do otherwise.
• either the researcher's initial assumption is correct and he experienced a very unusual event;
• or the researcher's initial assumption is incorrect.

In statistics, we generally don't make claims that require us to believe that a very unusual event happened. That is, in the practice of statistics, if the evidence (data) we collected is unlikely in light of the initial assumption, then we reject our initial assumption.

Example S.3.2

Criminal trial analogy section  .

One place where you can consistently see the general idea of hypothesis testing in action is in criminal trials held in the United States. Our criminal justice system assumes "the defendant is innocent until proven guilty." That is, our initial assumption is that the defendant is innocent.

In the practice of statistics, we make our initial assumption when we state our two competing hypotheses -- the null hypothesis ( H 0 ) and the alternative hypothesis ( H A ). Here, our hypotheses are:

• H 0 : Defendant is not guilty (innocent)
• H A : Defendant is guilty

In statistics, we always assume the null hypothesis is true . That is, the null hypothesis is always our initial assumption.

The prosecution team then collects evidence — such as finger prints, blood spots, hair samples, carpet fibers, shoe prints, ransom notes, and handwriting samples — with the hopes of finding "sufficient evidence" to make the assumption of innocence refutable.

In statistics, the data are the evidence.

The jury then makes a decision based on the available evidence:

• If the jury finds sufficient evidence — beyond a reasonable doubt — to make the assumption of innocence refutable, the jury rejects the null hypothesis and deems the defendant guilty. We behave as if the defendant is guilty.
• If there is insufficient evidence, then the jury does not reject the null hypothesis . We behave as if the defendant is innocent.

In statistics, we always make one of two decisions. We either "reject the null hypothesis" or we "fail to reject the null hypothesis."

Errors in Hypothesis Testing Section  

Did you notice the use of the phrase "behave as if" in the previous discussion? We "behave as if" the defendant is guilty; we do not "prove" that the defendant is guilty. And, we "behave as if" the defendant is innocent; we do not "prove" that the defendant is innocent.

This is a very important distinction! We make our decision based on evidence not on 100% guaranteed proof. Again:

• If we reject the null hypothesis, we do not prove that the alternative hypothesis is true.
• If we do not reject the null hypothesis, we do not prove that the null hypothesis is true.

We merely state that there is enough evidence to behave one way or the other. This is always true in statistics! Because of this, whatever the decision, there is always a chance that we made an error .

Let's review the two types of errors that can be made in criminal trials:

Table S.3.2 shows how this corresponds to the two types of errors in hypothesis testing.

Note that, in statistics, we call the two types of errors by two different  names -- one is called a "Type I error," and the other is called  a "Type II error." Here are the formal definitions of the two types of errors:

There is always a chance of making one of these errors. But, a good scientific study will minimize the chance of doing so!

Making the Decision Section  

Recall that it is either likely or unlikely that we would observe the evidence we did given our initial assumption. If it is likely , we do not reject the null hypothesis. If it is unlikely , then we reject the null hypothesis in favor of the alternative hypothesis. Effectively, then, making the decision reduces to determining "likely" or "unlikely."

In statistics, there are two ways to determine whether the evidence is likely or unlikely given the initial assumption:

• We could take the " critical value approach " (favored in many of the older textbooks).
• Or, we could take the " P -value approach " (what is used most often in research, journal articles, and statistical software).

In the next two sections, we review the procedures behind each of these two approaches. To make our review concrete, let's imagine that μ is the average grade point average of all American students who major in mathematics. We first review the critical value approach for conducting each of the following three hypothesis tests about the population mean $\mu$:

In Practice

• We would want to conduct the first hypothesis test if we were interested in concluding that the average grade point average of the group is more than 3.
• We would want to conduct the second hypothesis test if we were interested in concluding that the average grade point average of the group is less than 3.
• And, we would want to conduct the third hypothesis test if we were only interested in concluding that the average grade point average of the group differs from 3 (without caring whether it is more or less than 3).

Upon completing the review of the critical value approach, we review the P -value approach for conducting each of the above three hypothesis tests about the population mean \(\mu\). The procedures that we review here for both approaches easily extend to hypothesis tests about any other population parameter.

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

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• v.43(8); 2014 Dec

Hierarchy of hypotheses or cascade of predictions? A comment on Heger et al. (2013)

Alejandro g. farji-brener.

Laboratorio Ecotono, CRUB-Universidad Nacional del Comahue, INIBIOMA-CONICET. Pasaje Gutiérrez 1125, 8400 Bariloche, Argentina

Sabrina Amador-Vargas

Department of Integrative Biology, University of Texas at Austin, Austin, TX USA

The only way to test hypotheses is by evaluating their consequences. Since a hypothesis is an explanation of how nature works, it can be tested through the formulation of outcomes expected assuming the proposed hypothesis is true, and contrasting those predictions with the obtained results. Therefore, hypothesis and predictions are intrinsically different concepts. Hypotheses are ideas; predictions are expected results. Predictions are deduced from hypotheses, but it is unlikely to deduce a hypothesis from a prediction. Regardless of these conceptual differences, ecologists often formulate predictions but erroneously state them as hypothesis (Farji-Brener 2003 ). We believe that this is the case in the work of Heger et al. ( 2013 ). Here, we point out the confusion between hypotheses and predictions, highlight the importance of an adequate use of these terms, and propose the hierarchy-of-expected outcomes approach as an alternative to the hierarchy-of-hypotheses approach.

In the Heger et al. ( 2013 ) proposal, the authors highlight the potential of the hierarchy-of-hypotheses approach (hereafter, HoH) for testing the validity of biological hypotheses. Specifically, this method is used in the thorough study of Heger and Jeschke ( 2014 ) to evaluate the enemy release hypothesis (hereafter, ERH), a key idea in invasion ecology, which postulates that “ the absence of enemies in the exotic range is a cause of invasion success .” The HoH is a method that proposes partitioning a single major hypothesis in a hierarchy of sub-hypotheses. The general hypothesis is positioned at the top level, and it branches into more specific sub-hypotheses which also branch into even more specific sub-hypothesis. In this way, HoH integrates broad ideas as well as specific ones, which unify empirical tests under a common framework. The approach itself sounds promising, but its application facilitates the already common confusion between hypotheses and predictions.

The classification of sub-hypotheses in Heger et al. ( 2013 ) and Heger and Jeschke ( 2014 ) as it is presented ends up as a tool for organizing published results rather than a drawing-up of real hypotheses. To exemplify their method, the authors classified the sub-hypotheses of ERH according to three criteria: (a) indicator of enemy release, (b) type of comparison, and (c) type of enemies. However, these criteria are not real explanations derived from ERH of why invaders are successful. Criteria (a) and (c) focus on the response variable (e.g., leaf damage, performance of alien species, abundance or diversity of specialist or generalist natural enemies, etc.) and (b) focus on “treatments” (e.g., comparison between alien vs. native species, with or without enemies, etc.). In fact, the proposed sub-hypotheses are indeed predictions, because they are expected results rather than ideas. For example, the first subset of “hypotheses” (first branch level) is: “less damage by enemies,” “less infestation by enemies,” and “enhanced performance” (see Fig.  1 in Heger et al. 2013 , and Heger and Jeschke 2014 ); clearly, all those are expected results under the assumption that the ERH is true. The second-order level of “sub-hypotheses” specifies the treatments or units of comparison used in those predictions, but again, those are not potential explanations, as hypotheses should be. For instance, the second-level hypotheses for the first-level “leaf damage by enemies” specify whether the comparison was between alien vs. natives, alien in native vs. exotic range, etc. In other words, all these statements are specific predictions of the ERH (e.g., less damage by enemies in alien vs. natives, etc.), rather than sub-hypotheses.

Scheme of the hierarchy-of-expected outcomes approach (HoEOs). A hypothesis branches into different hierarchies of expected outcomes, which should be tested in order. If key assumptions are not supported by data, then testing outcomes at the lower level is worthless, and the original hypothesis could be rejected. Only one hypothesis and two assumptions are shown for simplicity

The confusion between hypotheses and predictions is more common than thought: about 30 % of ecology articles published in top journals used hypothesis and predictions as synonymous terms (Farji-Brener 2003 ). This confusion is not trivial, because it violates the principles of the hypothetic-deductive method. As discussed earlier, proposed explanations can only be tested by evaluating the expected results. Formulating predictions that lack explicit mention of the hypothesis they derived from impedes readers to assess the deductive capacity of the author. Specifically, omitting explicit hypotheses in the text (or replacing them with predictions) complicates its interpretation because readers ignore the tested explanation. Thus, it is impossible to critically assess whether relevant predictions were evaluated or whether non-mentioned alternative hypotheses are also valid. To avoid these complications, we propose the hierarchy-of-expected outcomes approach (HoEO) as an alternative to the hierarchy-of-hypotheses approach.

The HoEO method follows the general principles of the HoH but using expected outcomes (i.e., assumptions and predictions) instead of sub-hypotheses. Each hypothesis branches in expected results with different hierarchies based on their ability to undermine the proposed hypothesis (Fig.  1 .). If data do not support key assumptions (i.e., first-order expected outcomes), it is senseless to test specific predictions (i.e., second-order expected outcomes). Conversely, if assumptions are supported by data, we can proceed to test specific predictions, which may also branch in lower levels of hierarchy, relevance, or peculiarity depending on the topic. It is important to clarify that confirming assumptions does not necessarily verify the validity of the proposed hypothesis, but it is an essential step before continuing with the next level of expected outcomes. In other words, it is unreasonable to test specific predictions when key assumptions of the hypothesis are not supported by data. Returning to the ERH example, the first branch of assumptions could include: (a) a negative relationship between damage and fitness, and (b) the absence of natural enemies in the exotic range. Clearly, if damage by natural enemies does not reduce fitness or if natural enemies are present in the exotic range, the “absence” of enemies cannot be a cause of invasion success, and no further testing is needed. The second-level branch (i.e., predictions) may include those described as sub-hypothesis in Heger et al. ( 2013 ) and Heger and Jeschke ( 2014 ), for example, less damage in alien versus natives, in alien in native versus exotic ranges, etc. Specific predictions regarding type of damage, specificity of enemies, and proxies for fitness may be located at lower level branches.

This hierarchy-of-expected outcomes approach offers some conceptual and logistical advantages. First, it follows the hypothetic-deductive method, which postulates that ideas can only be proved through their consequences (but not vice versa) and that the strongest predictions are those with the best potential to discard the hypothesis they derived from. Second, authors must explicitly use their deductive abilities combined with the need for a deeper understanding of the natural history of the studied organism to formulate relevant predictions. Third, the hierarchy scheme avoids extra work: it is worthless to invest energy and time testing second-order expected outcomes when data did not support key assumptions. Fourth, this conceptual framework allows contrasting alternative hypotheses more clearly. A priori assignation of different weights to predictions within each hypothesis may help discriminate which hypothesis explains better a natural pattern. The system for weighting predictions and the decision of which hypothesis has more support may follow the method proposed by Heger and Jeschke ( 2014 ) to weight “sub-hypotheses”. We agree that general, broad hypotheses may be subdivided into more specific ones, as long as sub-hypotheses are not predictions. Also, it is clearer to contrast hypotheses by testing their assumptions and predictions to avoid conceptual confusions and to improve logistics of data collection. We propose that the hierarchy-of-outcomes approach could be a simple, practical, and useful tool to better understand how nature works.

Contributor Information

Alejandro G. Farji-Brener, Email: moc.oohay@ijrafela .

Sabrina Amador-Vargas, Email: ude.saxetu@vrodamas .

• Farji-Brener AG. Uso correcto, parcial e incorrecto de los términos hipótesis y predicciones en ecología. Ecología Austral. 2003; 13 :223–227. [ Google Scholar ]
• Heger T, Jeschke J. The enemy release hypothesis as a hierarchy of hypotheses. Oikos. 2014; 123 :741–750. doi: 10.1111/j.1600-0706.2013.01263.x. [ CrossRef ] [ Google Scholar ]
• Heger T, Botta-Dukát T, Gherardi P, Hoppe C, Hoste I, Jax K, Lindström L, Boets P, et al. Conceptual frameworks and methods for advancing invasion ecology. AMBIO. 2013; 42 :527–540. doi: 10.1007/s13280-012-0379-x. [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]

What Is a Hypothesis? (Science)

If...,Then...

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A hypothesis (plural hypotheses) is a proposed explanation for an observation. The definition depends on the subject.

In science, a hypothesis is part of the scientific method. It is a prediction or explanation that is tested by an experiment. Observations and experiments may disprove a scientific hypothesis, but can never entirely prove one.

In the study of logic, a hypothesis is an if-then proposition, typically written in the form, "If X , then Y ."

In common usage, a hypothesis is simply a proposed explanation or prediction, which may or may not be tested.

Writing a Hypothesis

Most scientific hypotheses are proposed in the if-then format because it's easy to design an experiment to see whether or not a cause and effect relationship exists between the independent variable and the dependent variable . The hypothesis is written as a prediction of the outcome of the experiment.

• Null Hypothesis and Alternative Hypothesis

Statistically, it's easier to show there is no relationship between two variables than to support their connection. So, scientists often propose the null hypothesis . The null hypothesis assumes changing the independent variable will have no effect on the dependent variable.

In contrast, the alternative hypothesis suggests changing the independent variable will have an effect on the dependent variable. Designing an experiment to test this hypothesis can be trickier because there are many ways to state an alternative hypothesis.

For example, consider a possible relationship between getting a good night's sleep and getting good grades. The null hypothesis might be stated: "The number of hours of sleep students get is unrelated to their grades" or "There is no correlation between hours of sleep and grades."

An experiment to test this hypothesis might involve collecting data, recording average hours of sleep for each student and grades. If a student who gets eight hours of sleep generally does better than students who get four hours of sleep or 10 hours of sleep, the hypothesis might be rejected.

But the alternative hypothesis is harder to propose and test. The most general statement would be: "The amount of sleep students get affects their grades." The hypothesis might also be stated as "If you get more sleep, your grades will improve" or "Students who get nine hours of sleep have better grades than those who get more or less sleep."

In an experiment, you can collect the same data, but the statistical analysis is less likely to give you a high confidence limit.

Usually, a scientist starts out with the null hypothesis. From there, it may be possible to propose and test an alternative hypothesis, to narrow down the relationship between the variables.

Example of a Hypothesis

Examples of a hypothesis include:

• If you drop a rock and a feather, (then) they will fall at the same rate.
• Plants need sunlight in order to live. (if sunlight, then life)
• Eating sugar gives you energy. (if sugar, then energy)
• White, Jay D.  Research in Public Administration . Conn., 1998.
• Schick, Theodore, and Lewis Vaughn.  How to Think about Weird Things: Critical Thinking for a New Age . McGraw-Hill Higher Education, 2002.
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