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Microbiology Research Proposal

2021, Assessment of Microbial Contamination on a used and disposed facemask.

Micro-organisms are ubiquitous and are found in almost every area around human bodies. Some are specifically found in certain regions of the body as a normal flora where they live as commensals with man. This association is important in protecting the body against other infectious diseases. Each area of the body surface acquires a characteristic flora of organisms well adapted to growth at that particular environment. These residents (normal flora) tend to suppress the intruders either by competition for space and food supply or by production of metabolites that are antagonistic to the survival of the intruder. These residents could be dislodged from their environment when sneezing, coughing, belching, yawning or could be destroyed by regular use of antiseptic soaps or creams on the body surfaces. Facemasks commonly used to prevent infectious diseases from airborne pathogens, therefore constitute an abode for bacteria. Furthermore, bacteria found in Facemasks could differ from one individual to another as the bacteria found could be a reflective of the environment and pathological conditions of the individual using the facemask.For instance, individual with upper respiratory tract infection are likely to dislodge strains of pathogenic microbes along sides with the normal flora in these regions. Enumeration of bacteria on used facemasks can be done using microscopic cell count and viable cell counting. Microscopic counts can be done on either samples dried on slides or samples in liquid. A viable cell counting is the one that is able to divide and form offspring. Viable cell counting is also called plate count and there are at least two ways of performing plate count: the spread plate and pour plate method. In spread plate method, a volume of appropriately diluted culture is spread over the surface of an agar plate using a sterile glass spreader. The plate is then incubated until colonies appear, and the number of colonies formed are counted.

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Microorganisms exist in a large and mixed population and therefore isolation of microorganisms to pure cultures will be the initial step in the study of the characteristics and potential of microorganisms. In nature, microbial populations do not segregate themselves by species but exist with a mixture of many other cell types. In the laboratory, these populations can be separated into pure cultures. These cultures contain only one type of organism and are suitable for the study of their cultural, morphological, and biochemical properties. In this experiment, you will first use one of the techniques designed to produce discrete colonies. Colonies are individual, macroscopically visible masses of microbial growth on a solid medium surface, each representing the multiplication of a single organism. Once you have obtained these discrete colonies, you will make an aseptic transfer onto nutrient agar slants for the isolation of pure cultures. Streak Plate : Isolation of Discrete Colonies from a Mixed Culture PRINCIPLE: The techniques commonly used for isolation of discrete colonies initially require that the number of organisms in the inoculum be reduced. The resulting diminution of the population size ensures that, following inoculation, individual cells will be sufficiently far apart on the surface of the agar medium to effect a separation of the different species present. The following are techniques that can be used to accomplish this necessary dilution: 1. The streak-plate method is a rapid qualitative isolation method. It is essentially a dilution technique that involves spreading a loopful of culture over the surface of an agar plate.

Elisa Lapadula

Lola Hogekamp

Alemayehu Choramo

In this practical laboratory works, three points were focused. These are: Anti-microbial susceptibility test, colony counting, and motility test. Each points discussed in detail as follows. Anti-microbial susceptibility test performed by using disc diffusion method to determine susceptibility of salmonella .In this test, kanamycin formed 24.26 mm ,ciprofloxacin formed 34.23 mm, and chloramphenicol formed 28.46 mmzone of inhibition, while sulphonamides formed no zone of inhibition. Colony counting was done by plate count method. Two medias were used. On VRB (violet red blue agar) media, with dilution of 10-2 was CFU/ml=3.5*103, with dilution of 10-3, CFU/ml=4.1*104 and with dilution of 10-4, CFU=1.5*105. But in this case also it is reported as "TFTC= too few to count". While on PCA (plate count agar) media, with dilution of 10-3 colony counted was 100, and CFU/ml=1.00*105,with dilution of 10-4 colony counted was 171, and CFU/ml=1.71*106 , with dilution of 10-5colony counted was 58, and CFU/ ml=5.8*106,with dilution of 10-6colony counted was 19, and CFU/ml=1.9*107,but in this case it is reported as "TFTC= to few to count". On the other hand, motility test was done in SIM (sulphure-indole-motility) and results obtained were: Staphylococcus aureus was non-motile; grow only on the line of stab, Escherichia. Coli spp. were motile, grow and move throughout the medium and Salmonella was both motile and produced gas.

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How to Write a Microbiology Research Proposal

How to Get a Ph.D. in Biology

Microbiology is the study of small organisms such as pathogens and bacteria. You can not see these organisms with your naked eye, you must use a microscope. Obtaining a degree in microbiology will prepare you for careers in pharmaceuticals, chemistry and quality control. A microbiology research proposal is a paper in which you choose a topic of interest and need, research it and present to a panel of reviewers as evidence of your knowledge of microbiology. This proposal is needed in completion in order to receive your graduate level degree in microbiology.

Clarify your topic. Make sure you provide enough information and background on your subject so anyone can follow and understand your proposal. The review panels may not be professionals in microbiology. Do not include unnecessary information away from the topic.

Write your proposal as if it were your final version. This will allow you to get constructive criticism that will help your final paper be the best possible.

Talk about your proposal with other colleagues. This will allow you to gather enough information to come up with two preproposals. Preproposals are one to two pages in length. One of your preproposals will become your chosen proposal topic.

Make sure you have all of the necessary pages of your proposal. Begin with the abstract. The abstract summarizes the points of your proposal in a short overview. Discuss the problem and how you will address it.

Discuss specific aims. Questions that your paper will answer are written in this section. Write it in outline format. It sets the outline of the experimental design portion of your proposal. Set realistic goals you can attain in two to three years in this section.

Discuss the background and significance. This is a lengthy section made up of several pages. This section makes the following sections clear to the panel. Cite former information about your profession that has already been published, here. You should also get across to your review panel that your proposal is important.

Discuss previous results. This shows your panel you are working on your proposal by providing them with statistics and information you have gathered thus far.

Write the experimental design section. This section lets your panel know you understand your proposal and you will have readable results. Here you will defend your work in detail. Make your goals and priorities apparent here.

Create a timetable. In this brief section let the panel know when you anticipate your proposal will be finished.

Create a list of literature cited. This is a standard reference page in which you list your sources.

Make sure your microbiology research proposal meets standard National Institute of Health format guidelines.

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What’s Included: Research Proposal Template

Our free dissertation/thesis proposal template covers the core essential ingredients for a strong research proposal. It includes clear explanations of what you need to address in each section, as well as straightforward examples and links to further resources.

The research proposal template covers the following core elements:

• Introduction & background (including the research problem)
• Literature review
• Research design / methodology
• Project plan , resource requirements and risk management

The cleanly-formatted Google Doc can be downloaded as a fully editable MS Word Document (DOCX format), so you can use it as-is or convert it to LaTeX.

PS – if you’d like a high-level template for the entire thesis, you can we’ve got that too .

Research Proposal Template FAQS

What types of research proposals can this template be used for.

The proposal template follows the standard format for academic research projects, which means it will be suitable for the vast majority of dissertations and theses (especially those within the sciences), whether they are qualitative or quantitative in terms of design.

Keep in mind that the exact requirements for the introduction chapter/section will vary between universities and degree programs. These are typically minor, but it’s always a good idea to double-check your university’s requirements before you finalise your structure.

Is this template for an undergrad, Master or PhD-level proposal?

This template can be used for a research project at any level of study. Doctoral-level projects typically require the research proposal to be more extensive/comprehensive, but the structure will typically remain the same.

How long should my research proposal be?

The length of a research proposal varies by institution and subject, but as a ballpark, it’s usually between 1,500 and 3,000 words.

To be safe, it’s best to check with your university if they have any preferences or requirements in terms of minimum and maximum word count for the research propsal.

How detailed should the methodology of the proposal be?

You don’t need to go into the fine details of your methodology, but this section should be detailed enough to demonstrate that your research approach is feasible and will address your research questions effectively. Be sure to include your intended methods for data collection and analysis.

Can I include preliminary data or pilot study results in my proposal?

Generally, yes. This can strengthen your proposal by demonstrating the feasibility of your research. However, make sure that your pilot study is approved by your university before collecting any data.

Can I share this template with my friends/colleagues?

Yes, you’re welcome to share this template in its original format (no editing allowed). If you want to post about it on your blog or social media, we kindly request that you reference this page as your source.

What format is the template (DOC, PDF, PPT, etc.)?

The research proposal template is provided as a Google Doc. You can download it in MS Word format or make a copy to your Google Drive. You’re also welcome to convert it to whatever format works best for you, such as LaTeX or PDF.

Do you have templates for the other chapters?

Yes, we do. We are constantly developing our collection of free resources to help students complete their dissertations and theses. You can view all of our template resources here .

Can Grad Coach help me with my dissertation/thesis?

Yes, you’re welcome to get in touch with us to discuss our private coaching services .

Further Resources: Proposal Writing

The template provides step-by-step guidance for each section of your research proposal, but if you’d like to learn more about how to write up a high-quality research proposal, check out the rest of our free proposal-related resources:

• Research Proposal 101
• Examples of research proposals
• How To Find A Research Topic
• How To Find A Research Gap
• Developing Your Golden Thread
• How To Write A Research Proposal
• 8 Common Proposal Writing Mistakes

You can also visit the Grad Coach blog for more proposal-related resources.

If you’d prefer 1-on-1 support with your research proposal, have a look at our private coaching service , where we hold your hand through the research process, step by step.

BYU ScholarsArchive

Home > Life Sciences > Microbiology and Molecular Biology > Theses and Dissertations

Microbiology and Molecular Biology Theses and Dissertations

Theses/dissertations from 2024 2024.

Characterization of Cellular Metabolism Regulation by the Transcription Factor Centromere Binding Factor 1 (Cbf1) , Spencer Ellsworth

Theses/Dissertations from 2023 2023

Elucidating the Architecture of the TclIJN Complex that Converts Cysteine to Thiazoles in the Biosynthesis of Micrococcin , Diana G. Calvopina Chavez

Manipulating and Assaying Chromatin Architecture Around Enhancer Elements in vivo , John Lawrence Carter

Halophilic Genes that Impact Plant Growth in Saline Soils , Mckay A. Meinzer

Characterizing Stress Granule Regulation by PAS Kinase, Ataxin-2 and Ptc6 and Investigating the Lifespan of Covid-19 Virus on Currency , Colleen R. Newey

Changes in RNA Expression of HuT78 Cells Resulting From the HIV-1 Viral Protein R R77Q Mutation , Joshua S. Ramsey

Theses/Dissertations from 2022 2022

Biofilm Characterization and the Potential Role of eDNA in Horizontal Gene Transfer in Hospital and Meat Isolates of Staphylococcus aureus and Their Biofilms , Ashley Lynne Ball

Novel Patterns for Nucleosome Positioning: From in vitro to in vivo , David Andrew Bates

The Effects of Polymorphisms of Viral Protein R of HIV-1 on the Induction of Apoptosis in Primary Cells and the Characterization of Twelve Novel Bacillus anthracis Bacteriophage , Jacob D. Fairholm

Analysis of the Cytopathogenic Effect of Different HIV-1 Vpr Isoforms on Primary Human CD4+ T Cells and a Model Cell Line , Jonatan Josue Fierro Nieves

The Role of Chitinase A in Mastitis-Associated Escherichia coli Pathogenesis , Weston D. Hutchison

Big Data Meta-Analyses of Transcriptional Responses of Human Samples to Orthohantavirus Infection and Shotgun Metagenomics From Crohn's Disease Patients. , John L. Krapohl

An Exploration of Factors that Impact Uptake of Human Papillomavirus Vaccines , David Samuel Redd

Genomic Analysis and Therapeutic Development of Bacteriophages to Treat Bacterial Infections and Parasitic Infestations , Daniel W. Thompson

The Use of Nucleotide Salvage Pathway Enzymes as Suitable Tumor Targets for Antibody-Based and Adoptive Cell Therapies , Edwin J. Velazquez

Comparative Sequence Analysis Elucidates the Evolutionary Patterns of Yersinia pestis in New Mexico over Thirty-Two Years , M. Elizabeth Warren

Regulation of T Cell Activation by the CD5 Co-Receptor and Altered Peptides, Characterization of Thymidine Kinase-Specific Antibodies, and Integrating Genomics Education in Society , Kiara Vaden Whitley

Theses/Dissertations from 2021 2021

Evolution and Selection: From Suppression of Metabolic Deficiencies to Bacteriophage Host Range and Resistance , Daniel Kurt Arens

Identifying Sinorhizobium meliloti Genes that Determine Fitness Outcomes , Alexander B. Benedict

Pushing the Limits of SARS-CoV-2 Survival: How SARS-CoV-2 Responds to Quaternary Ammonium Compounds and Wastewater , Benjamin Hawthorne Ogilvie

Mutations in HIV-1 Vpr Affect Pathogenesis in T-Lymphocytes and Novel Strategies to Contain the Current COVID-19 Pandemic , Antonio Solis Leal

Theses/Dissertations from 2020 2020

Staphylococcus aureus Metal Acquisition in Milk and Mammary Gland Tissue , Shalee Killpack Carlson

Antimicrobial Peptide Development: From Massively Parallel Peptide Sequencing to Bioinformatic Motif Identification , Alexander K. Erikson

A Comparison of Chikungunya Virus Infection, Dissemination, and Cytokine Induction in Human and Murine Macrophages and Characterization of RAG2-/-γc-/- Mice as an Animal Model to Study Neurotropic Chikungunya Disease , Israel Guerrero

The Effects of Immune Regulation and Dysregulation: Helper T Cell Receptor Affinity, Systemic Lupus Erythematosus and Cancer Risk, and Vaccine Hesitancy , Deborah K. Johnson

Identification of Genes that Determine Fitness, Virulence, and Disease Outcomes in Mastitis Associated Eschericia coli , Michael Andrew Olson

Theses/Dissertations from 2019 2019

Investigation of Thymidine Kinase 1 in Cancer Progression , Eliza Esther King Bitter

Ribosomally Synthesized and Post-Translationally Modified Peptides as Potential Scaffolds for Peptide Engineering , Devan Bursey

Bioaerosols Associated with Evaporative Cooler Use in Low-Income Homes in Semi-Arid Climates , Ashlin Elaine Cowger

PAS Kinase and TOR, Controllers of Cell Growth and Proliferation , Brooke Jasmyn Cozzens

Regulation of Immune Cell Activation and Functionby the nBMPp2 Protein andthe CD5 Co-Receptor , Claudia Mercedes Freitas

Characterizing Novel Pathways for Regulation and Function of Ataxin-2 , Elise Spencer Melhado

Interactions Between the Organellar Pol1A, Pol1B, and Twinkle DNA Replication Proteins and Their Role in Plant Organelle DNA Replication , Stewart Anthony Morley

SNFing Glucose to PASs Mitochondrial Dysfunction: The Role of Two Sensory Protein Kinases in Metabolic Diseases , Kai Li Ong

Characterizing the Function of PAS kinase in Cellular Metabolism and Neurodegenerative Disease , Jenny Adele Pape

Isolation, Characterization, and Genomic Comparison of Bacteriophages of Enterobacteriales Order , Ruchira Sharma

Isolation, Genetic Characterization and Clinical Application of Bacteriophages of Pathogenic Bacterial Species , Trever Leon Thurgood

Investigation of Therapeutic Immune Cell Metabolism , Josephine Anna Tueller

Theses/Dissertations from 2018 2018

Innate Immune Cell Phenotypes Are Dictated by Distinct Epigenetic Reprogramming , Kevin Douglas Adams

Bacteriophages for Treating American Foulbrood and the Neutralization of Paenibacillus larvae Spores , Thomas Scott Brady

Methods for Detection of and Therapy for Carbapenem-Resistant Enterobacteriaceae , Olivia Tateoka Brown

The Diversity Found Among Carbapenem-Resistant Bacteria , Galen Edward Card

Exploration of Antimicrobial Activity in Natural Peptides and High-Throughput Discovery of Synthetic Peptides , Emma Kay Dallon

Gut Microbiota Regulates the Interplay Between Diet and Genetics to Influence Insulin Resistance , Jeralyn Jones Franson

The Antimicrobial Properties of Honey and Their Effect on Pathogenic Bacteria , Shreena Himanshu Mody

The Ability of Novel Phage to Infect Virulent Bacillus anthracis Isolates , Hyrum Smith Shumway

Galleria Mellonella as an Alternate Infection Model for Burkholderia Species and a Comparison of Suspension and Surface Test Methods for Evaluating Sporicidal Efficacy , Joseph D. Thiriot

The Clinical Significance of HPRT as a Diagnostic and Therapeutic Biomarker for Hematological and Solid Malignancies , Michelle Hannah Townsend

Biomarker Analysis and Clinical Relevance of Thymidine Kinase 1 in Solid and Hematological Malignancies , Evita Giraldez Weagel

Hospital and meat associated Staphylococcus aureus and Their Biofilm Characteristics , Trevor Michael Wienclaw

Theses/Dissertations from 2017 2017

Comparison of Cytokine Expression and Bacterial Growth During Periparturient and Mid Lactation Mastitis in a Mouse Model , Rhonda Nicole Chronis

Influence of Epstein-Barr Virus on Systemic Lupus Erythematosus Disease Development and the Role of Depression on Disease Progression , Caleb Cornaby

The Effects of Nucleosome Positioning and Chromatin Architecture on Transgene Expression , Colton E. Kempton

Phosphate Signaling Through Alternate Conformations of the PstSCAB Phosphate Transporter , Ramesh Krishna Vuppada

Acetobacter fabarum Genes Influencing Drosophila melanogaster Phenotypes , Kylie MaKay White

Theses/Dissertations from 2016 2016

The Path to Understanding Salt Tolerance: Global Profiling of Genes Using Transcriptomics of the Halophyte Suaeda fruticosa , Joann Diray Arce

Genetic and Biochemical Analysis of the Micrococcin Biosynthetic Pathway , Philip Ross Bennallack

Characterizing Interaction Between PASK and PBP1/ ATXN2 to Regulate Cell Growth and Proliferation , Nidhi Rajan Choksi

The Activity of Alkaline Glutaraldehyde Against Bacterial Endospores and Select Non-Enveloped Viruses , Justen Thalmus Despain

The Role of Viral Interleukin-6 in Tumor Development of Kaposi's Sarcoma-Associated Herpesvirus Lymphomas , Rebecca A. Fullwood

The Role of the Transcriptional Antiterminator RfaH in Lipopolysaccharide Synthesis, Resistance to Antimicrobial Peptides, and Virulence of Yersinia pseudotuberculosis and Yersinia pestis , Jared Michael Hoffman

A CryAB Interactome Reveals Clientele Specificity and Dysfunction of Mutants Associated with Human Disease , Whitney Katherine Hoopes

The pmrHFIJKLM Operon in Yersinia pseudotuberculosis Enhances Resistance to CCL28 and Promotes Phagocytic Engulfment by Neutrophils , Lauren Elizabeth Johnson

Characterization of Five Brevibacillus Bacteriophages and Their Genomes , Michael Allen Sheflo

Analysis of Nucleosome Isolation and Recovery: From In Silico Invitrosomes to In Vivo Nucleosomes , Collin Brendan Skousen

Human Herpesvirus 6A Infection and Immunopathogenesis in Humanized Rag2 -/-γc-/- Mice and Relevance to HIV/AIDS and Autoimmunity , Anne Tanner

Theses/Dissertations from 2015 2015

Identifying and Characterizing Yeast PAS Kinase 1 Substrates Reveals Regulation of Mitochondrial and Cell Growth Pathways , Desiree DeMille

The Detection and Molecular Evolution of Francisella tularensis Subspecies , Mark K. Gunnell

Isolation and Host Range of Staphylococcus aureus Bacteriophages and Use for Decontamination of Fomites , Kyle C. Jensen

The Antioxidant and DNA Repair Capacities of Resveratrol, Piceatannol, and Pterostilbene , Justin Ryan Livingston

High Salinity Stabilizes Bacterial Community Composition and Activity Through Time , Tylan Wayne Magnusson

Advancing Phage Genomics and Honeybee Health Through Discovery and Characterization of Paenibacillaceae Bacteriophages , Bryan Douglas Merrill

Specialized Replication Operons Control Rhizobial Plasmid Copy Number in Developing Symbiotic Cells , Clarice Lorraine Perry

Gene Networks Involved in Competitive Root Colonization and Nodulation in the Sinorhizobium meliloti-Medicago truncatula Symbiosis , Ryan D. VanYperen

Theses/Dissertations from 2014 2014

Snf1 Mediated Phosphorylation and Activation of PAS Kinase , Bryan D. Badal

Studies of PhoU in Escherichia coli: Metal Binding, Dimerization,Protein/Protein Interactions, and a Signaling Complex Model , Stewart G. Gardner

Pharmacologic Immunomodulation of Macrophage Activation by Caffeine , Ryan Perry Steck

Analysis of Nucleosome Mobility, Fragility, and Recovery: From Embryonic Stem Cells to Invitrosomes , Ashley Nicolle Wright

Enhancing Protein and Enzyme Stability Through Rationally Engineered Site-Specific Immobilization Utilizing Non-Canonical Amino Acids , Jeffrey Chun Wu

Theses/Dissertations from 2013 2013

Thymidine Kinase 1: Diagnostic and Prognostic Significance in Malignancy , Melissa Marie Alegre

Promoter Polymorphisms in Interferon Regulatory Factor 5 , Daniel N. Clark

Modulators of Symbiotic Outcome in Sinorhizobium meliloti , Matthew B. Crook

Evidences for Protein-Protein Interactions Between PstB and PhoU in the Phosphate Signaling Complex of Escherichia coli , Kristine Dawn Johns

Identification of the Binding Partners for HspB2 and CryAB Reveals Myofibril and Mitochondrial Protein Interactions and Non-Redundant Roles for Small Heat Shock Proteins , Kelsey Murphey Langston

A Quadruplex Real-Time PCR Assay for the Rapid Detection and Differentiation of the Burkholderia pseudomallei Complex: B. mallei , B. pseudomallei , and B. thailandensis , Chinn-woan Lowe

The Role of Nuclear BMP2 in the Cell Cycle and Tumorigenesis , Brandt Alan Nichols

Nuclear BMP2 and the Immune Response , Daniel S. Olsen

Hypersaline Lake Environments Exhibit Reduced Microbial Dormancy , Joshua Christopher Vert

Theses/Dissertations from 2012 2012

Characterization of the Cellular and Organellar Dynamics that Occur with a Partial Depletion of Mitochondrial DNA when Arabidopsis Organellar DNA Polymerase IB is Mutated , John D. Cupp

Effect of Antioxidants and Oxidative Stress on Different Cancer Cell Types , Gaytri Devi Gupta Elera

Effects of Chemical Stimulation and Tumor Co-Incubation on Macrophage Activation and Aggressiveness, Measured Through Phagocytosis and Respiratory Burst , Bo Marcus Gustafsson

Loss of the Lipopolysaccharide Core Biosynthesis rfaD Gene Increases Antimicrobial Chemokine Binding and Bacterial Susceptibility to CCL28 and Polymyxin: A Model for Understanding the Interface of Antimicrobial Chemokines and Bacterial Host Defense Avoidance Mechanisms , Cynthia S. Lew

Partial Characterization of the Antimicrobial Activity of CCL28 , Bin Liu

Characterizing the Role of HspB2 in Cardiac Metabolism and Muscle Structure Using Yeast and Mammalian Systems , Jonathan Paul Neubert

Humanized Mice as a Model to Study Human Viral Pathogenesis and Novel Antiviral Drugs , Freddy Mauricio Sanchez Tumbaco

Transgene Delivery via Microelectromechanical Systems , Aubrey Marie Mueller Wilson

Theses/Dissertations from 2011 2011

Antioxidants in Cancer Research and Prevention: Assay Comparison, Structure-Function Analysis, and Food Product Analysis , Andrew Robert Garrett

Characterization of the Role Nuclear Bmp2 (nBmp2) Plays in Regulating Gene Expression , Fialka Grigorova

Theses/Dissertations from 2010 2010

Effects of Diabetic State and Gender on Pro-Inflammatory Cytokine Secretion by Human Macrophages Infected with Burkholderia pseudomallei , Annette J. Blam

Organellar DNA Polymerases Gamma I and II in Arabidopsis thaliana , Jeffrey M. Brammer

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