example of hypothesis with two independent variables

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8.2 Multiple Independent Variables

Learning objectives.

• Explain why researchers often include multiple independent variables in their studies.
• Define factorial design, and use a factorial design table to represent and interpret simple factorial designs.
• Distinguish between main effects and interactions, and recognize and give examples of each.
• Sketch and interpret bar graphs and line graphs showing the results of studies with simple factorial designs.

Just as it is common for studies in psychology to include multiple dependent variables, it is also common for them to include multiple independent variables. Schnall and her colleagues studied the effect of both disgust and private body consciousness in the same study. Researchers’ inclusion of multiple independent variables in one experiment is further illustrated by the following actual titles from various professional journals:

• The Effects of Temporal Delay and Orientation on Haptic Object Recognition
• Opening Closed Minds: The Combined Effects of Intergroup Contact and Need for Closure on Prejudice
• Effects of Expectancies and Coping on Pain-Induced Intentions to Smoke
• The Effect of Age and Divided Attention on Spontaneous Recognition
• The Effects of Reduced Food Size and Package Size on the Consumption Behavior of Restrained and Unrestrained Eaters

Just as including multiple dependent variables in the same experiment allows one to answer more research questions, so too does including multiple independent variables in the same experiment. For example, instead of conducting one study on the effect of disgust on moral judgment and another on the effect of private body consciousness on moral judgment, Schnall and colleagues were able to conduct one study that addressed both questions. But including multiple independent variables also allows the researcher to answer questions about whether the effect of one independent variable depends on the level of another. This is referred to as an interaction between the independent variables. Schnall and her colleagues, for example, observed an interaction between disgust and private body consciousness because the effect of disgust depended on whether participants were high or low in private body consciousness. As we will see, interactions are often among the most interesting results in psychological research.

Factorial Designs

By far the most common approach to including multiple independent variables in an experiment is the factorial design. In a factorial design , each level of one independent variable (which can also be called a factor ) is combined with each level of the others to produce all possible combinations. Each combination, then, becomes a condition in the experiment. Imagine, for example, an experiment on the effect of cell phone use (yes vs. no) and time of day (day vs. night) on driving ability. This is shown in the factorial design table in Figure 8.2 “Factorial Design Table Representing a 2 × 2 Factorial Design” . The columns of the table represent cell phone use, and the rows represent time of day. The four cells of the table represent the four possible combinations or conditions: using a cell phone during the day, not using a cell phone during the day, using a cell phone at night, and not using a cell phone at night. This particular design is a 2 × 2 (read “two-by-two”) factorial design because it combines two variables, each of which has two levels. If one of the independent variables had a third level (e.g., using a handheld cell phone, using a hands-free cell phone, and not using a cell phone), then it would be a 3 × 2 factorial design, and there would be six distinct conditions. Notice that the number of possible conditions is the product of the numbers of levels. A 2 × 2 factorial design has four conditions, a 3 × 2 factorial design has six conditions, a 4 × 5 factorial design would have 20 conditions, and so on.

Figure 8.2 Factorial Design Table Representing a 2 × 2 Factorial Design

In principle, factorial designs can include any number of independent variables with any number of levels. For example, an experiment could include the type of psychotherapy (cognitive vs. behavioral), the length of the psychotherapy (2 weeks vs. 2 months), and the sex of the psychotherapist (female vs. male). This would be a 2 × 2 × 2 factorial design and would have eight conditions. Figure 8.3 “Factorial Design Table Representing a 2 × 2 × 2 Factorial Design” shows one way to represent this design. In practice, it is unusual for there to be more than three independent variables with more than two or three levels each because the number of conditions can quickly become unmanageable. For example, adding a fourth independent variable with three levels (e.g., therapist experience: low vs. medium vs. high) to the current example would make it a 2 × 2 × 2 × 3 factorial design with 24 distinct conditions. In the rest of this section, we will focus on designs with two independent variables. The general principles discussed here extend in a straightforward way to more complex factorial designs.

Figure 8.3 Factorial Design Table Representing a 2 × 2 × 2 Factorial Design

Assigning Participants to Conditions

Recall that in a simple between-subjects design, each participant is tested in only one condition. In a simple within-subjects design, each participant is tested in all conditions. In a factorial experiment, the decision to take the between-subjects or within-subjects approach must be made separately for each independent variable. In a between-subjects factorial design , all of the independent variables are manipulated between subjects. For example, all participants could be tested either while using a cell phone or while not using a cell phone and either during the day or during the night. This would mean that each participant was tested in one and only one condition. In a within-subjects factorial design , all of the independent variables are manipulated within subjects. All participants could be tested both while using a cell phone and while not using a cell phone and both during the day and during the night. This would mean that each participant was tested in all conditions. The advantages and disadvantages of these two approaches are the same as those discussed in Chapter 6 “Experimental Research” . The between-subjects design is conceptually simpler, avoids carryover effects, and minimizes the time and effort of each participant. The within-subjects design is more efficient for the researcher and controls extraneous participant variables.

It is also possible to manipulate one independent variable between subjects and another within subjects. This is called a mixed factorial design . For example, a researcher might choose to treat cell phone use as a within-subjects factor by testing the same participants both while using a cell phone and while not using a cell phone (while counterbalancing the order of these two conditions). But he or she might choose to treat time of day as a between-subjects factor by testing each participant either during the day or during the night (perhaps because this only requires them to come in for testing once). Thus each participant in this mixed design would be tested in two of the four conditions.

Regardless of whether the design is between subjects, within subjects, or mixed, the actual assignment of participants to conditions or orders of conditions is typically done randomly.

Nonmanipulated Independent Variables

In many factorial designs, one of the independent variables is a nonmanipulated independent variable . The researcher measures it but does not manipulate it. The study by Schnall and colleagues is a good example. One independent variable was disgust, which the researchers manipulated by testing participants in a clean room or a messy room. The other was private body consciousness, which the researchers simply measured. Another example is a study by Halle Brown and colleagues in which participants were exposed to several words that they were later asked to recall (Brown, Kosslyn, Delamater, Fama, & Barsky, 1999). The manipulated independent variable was the type of word. Some were negative health-related words (e.g., tumor , coronary ), and others were not health related (e.g., election , geometry ). The nonmanipulated independent variable was whether participants were high or low in hypochondriasis (excessive concern with ordinary bodily symptoms). The result of this study was that the participants high in hypochondriasis were better than those low in hypochondriasis at recalling the health-related words, but they were no better at recalling the non-health-related words.

Such studies are extremely common, and there are several points worth making about them. First, nonmanipulated independent variables are usually participant variables (private body consciousness, hypochondriasis, self-esteem, and so on), and as such they are by definition between-subjects factors. For example, people are either low in hypochondriasis or high in hypochondriasis; they cannot be tested in both of these conditions. Second, such studies are generally considered to be experiments as long as at least one independent variable is manipulated, regardless of how many nonmanipulated independent variables are included. Third, it is important to remember that causal conclusions can only be drawn about the manipulated independent variable. For example, Schnall and her colleagues were justified in concluding that disgust affected the harshness of their participants’ moral judgments because they manipulated that variable and randomly assigned participants to the clean or messy room. But they would not have been justified in concluding that participants’ private body consciousness affected the harshness of their participants’ moral judgments because they did not manipulate that variable. It could be, for example, that having a strict moral code and a heightened awareness of one’s body are both caused by some third variable (e.g., neuroticism). Thus it is important to be aware of which variables in a study are manipulated and which are not.

Graphing the Results of Factorial Experiments

The results of factorial experiments with two independent variables can be graphed by representing one independent variable on the x- axis and representing the other by using different kinds of bars or lines. (The y- axis is always reserved for the dependent variable.) Figure 8.4 “Two Ways to Plot the Results of a Factorial Experiment With Two Independent Variables” shows results for two hypothetical factorial experiments. The top panel shows the results of a 2 × 2 design. Time of day (day vs. night) is represented by different locations on the x- axis, and cell phone use (no vs. yes) is represented by different-colored bars. (It would also be possible to represent cell phone use on the x- axis and time of day as different-colored bars. The choice comes down to which way seems to communicate the results most clearly.) The bottom panel of Figure 8.4 “Two Ways to Plot the Results of a Factorial Experiment With Two Independent Variables” shows the results of a 4 × 2 design in which one of the variables is quantitative. This variable, psychotherapy length, is represented along the x- axis, and the other variable (psychotherapy type) is represented by differently formatted lines. This is a line graph rather than a bar graph because the variable on the x- axis is quantitative with a small number of distinct levels.

Figure 8.4 Two Ways to Plot the Results of a Factorial Experiment With Two Independent Variables

Main Effects and Interactions

In factorial designs, there are two kinds of results that are of interest: main effects and interaction effects (which are also called just “interactions”). A main effect is the statistical relationship between one independent variable and a dependent variable—averaging across the levels of the other independent variable. Thus there is one main effect to consider for each independent variable in the study. The top panel of Figure 8.4 “Two Ways to Plot the Results of a Factorial Experiment With Two Independent Variables” shows a main effect of cell phone use because driving performance was better, on average, when participants were not using cell phones than when they were. The blue bars are, on average, higher than the red bars. It also shows a main effect of time of day because driving performance was better during the day than during the night—both when participants were using cell phones and when they were not. Main effects are independent of each other in the sense that whether or not there is a main effect of one independent variable says nothing about whether or not there is a main effect of the other. The bottom panel of Figure 8.4 “Two Ways to Plot the Results of a Factorial Experiment With Two Independent Variables” , for example, shows a clear main effect of psychotherapy length. The longer the psychotherapy, the better it worked. But it also shows no overall advantage of one type of psychotherapy over the other.

There is an interaction effect (or just “interaction”) when the effect of one independent variable depends on the level of another. Although this might seem complicated, you have an intuitive understanding of interactions already. It probably would not surprise you, for example, to hear that the effect of receiving psychotherapy is stronger among people who are highly motivated to change than among people who are not motivated to change. This is an interaction because the effect of one independent variable (whether or not one receives psychotherapy) depends on the level of another (motivation to change). Schnall and her colleagues also demonstrated an interaction because the effect of whether the room was clean or messy on participants’ moral judgments depended on whether the participants were low or high in private body consciousness. If they were high in private body consciousness, then those in the messy room made harsher judgments. If they were low in private body consciousness, then whether the room was clean or messy did not matter.

The effect of one independent variable can depend on the level of the other in different ways. This is shown in Figure 8.5 “Bar Graphs Showing Three Types of Interactions” . In the top panel, one independent variable has an effect at one level of the second independent variable but no effect at the others. (This is much like the study of Schnall and her colleagues where there was an effect of disgust for those high in private body consciousness but not for those low in private body consciousness.) In the middle panel, one independent variable has a stronger effect at one level of the second independent variable than at the other level. This is like the hypothetical driving example where there was a stronger effect of using a cell phone at night than during the day. In the bottom panel, one independent variable again has an effect at both levels of the second independent variable, but the effects are in opposite directions. Figure 8.5 “Bar Graphs Showing Three Types of Interactions” shows the strongest form of this kind of interaction, called a crossover interaction . One example of a crossover interaction comes from a study by Kathy Gilliland on the effect of caffeine on the verbal test scores of introverts and extroverts (Gilliland, 1980). Introverts perform better than extroverts when they have not ingested any caffeine. But extroverts perform better than introverts when they have ingested 4 mg of caffeine per kilogram of body weight. Figure 8.6 “Line Graphs Showing Three Types of Interactions” shows examples of these same kinds of interactions when one of the independent variables is quantitative and the results are plotted in a line graph. Note that in a crossover interaction, the two lines literally “cross over” each other.

Figure 8.5 Bar Graphs Showing Three Types of Interactions

In the top panel, one independent variable has an effect at one level of the second independent variable but not at the other. In the middle panel, one independent variable has a stronger effect at one level of the second independent variable than at the other. In the bottom panel, one independent variable has the opposite effect at one level of the second independent variable than at the other.

Figure 8.6 Line Graphs Showing Three Types of Interactions

In many studies, the primary research question is about an interaction. The study by Brown and her colleagues was inspired by the idea that people with hypochondriasis are especially attentive to any negative health-related information. This led to the hypothesis that people high in hypochondriasis would recall negative health-related words more accurately than people low in hypochondriasis but recall non-health-related words about the same as people low in hypochondriasis. And of course this is exactly what happened in this study.

Key Takeaways

• Researchers often include multiple independent variables in their experiments. The most common approach is the factorial design, in which each level of one independent variable is combined with each level of the others to create all possible conditions.
• In a factorial design, the main effect of an independent variable is its overall effect averaged across all other independent variables. There is one main effect for each independent variable.
• There is an interaction between two independent variables when the effect of one depends on the level of the other. Some of the most interesting research questions and results in psychology are specifically about interactions.
• Practice: Return to the five article titles presented at the beginning of this section. For each one, identify the independent variables and the dependent variable.
• Practice: Create a factorial design table for an experiment on the effects of room temperature and noise level on performance on the SAT. Be sure to indicate whether each independent variable will be manipulated between subjects or within subjects and explain why.

Brown, H. D., Kosslyn, S. M., Delamater, B., Fama, A., & Barsky, A. J. (1999). Perceptual and memory biases for health-related information in hypochondriacal individuals. Journal of Psychosomatic Research , 47 , 67–78.

Gilliland, K. (1980). The interactive effect of introversion-extroversion with caffeine induced arousal on verbal performance. Journal of Research in Personality , 14 , 482–492.

Research Methods in Psychology Copyright © 2016 by University of Minnesota is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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Chapter 8: Complex Research Designs

Multiple Independent Variables

Learning Objectives

• Explain why researchers often include multiple independent variables in their studies.
• Define factorial design, and use a factorial design table to represent and interpret simple factorial designs.
• Distinguish between main effects and interactions, and recognize and give examples of each.
• Sketch and interpret bar graphs and line graphs showing the results of studies with simple factorial designs.

Just as it is common for studies in psychology to include multiple dependent variables, it is also common for them to include multiple independent variables. Schnall and her colleagues studied the effect of both disgust and private body consciousness in the same study. Researchers’ inclusion of multiple independent variables in one experiment is further illustrated by the following actual titles from various professional journals:

• The Effects of Temporal Delay and Orientation on Haptic Object Recognition
• Opening Closed Minds: The Combined Effects of Intergroup Contact and Need for Closure on Prejudice
• Effects of Expectancies and Coping on Pain-Induced Intentions to Smoke
• The Effect of Age and Divided Attention on Spontaneous Recognition
• The Effects of Reduced Food Size and Package Size on the Consumption Behaviour of Restrained and Unrestrained Eaters

Just as including multiple dependent variables in the same experiment allows one to answer more research questions, so too does including multiple independent variables in the same experiment. For example, instead of conducting one study on the effect of disgust on moral judgment and another on the effect of private body consciousness on moral judgment, Schnall and colleagues were able to conduct one study that addressed both questions. But including multiple independent variables also allows the researcher to answer questions about whether the effect of one independent variable depends on the level of another. This is referred to as an interaction between the independent variables. Schnall and her colleagues, for example, observed an interaction between disgust and private body consciousness because the effect of disgust depended on whether participants were high or low in private body consciousness. As we will see, interactions are often among the most interesting results in psychological research.

Factorial Designs

By far the most common approach to including multiple independent variables in an experiment is the factorial design. In a  factorial design , each level of one independent variable (which can also be called a  factor ) is combined with each level of the others to produce all possible combinations. Each combination, then, becomes a condition in the experiment. Imagine, for example, an experiment on the effect of cell phone use (yes vs. no) and time of day (day vs. night) on driving ability. This is shown in the  factorial design table  in Figure 8.1. The columns of the table represent cell phone use, and the rows represent time of day. The four cells of the table represent the four possible combinations or conditions: using a cell phone during the day, not using a cell phone during the day, using a cell phone at night, and not using a cell phone at night. This particular design is referred to as a 2 × 2 (read “two-by-two”) factorial design because it combines two variables, each of which has two levels. If one of the independent variables had a third level (e.g., using a handheld cell phone, using a hands-free cell phone, and not using a cell phone), then it would be a 3 × 2 factorial design, and there would be six distinct conditions. Notice that the number of possible conditions is the product of the numbers of levels. A 2 × 2 factorial design has four conditions, a 3 × 2 factorial design has six conditions, a 4 × 5 factorial design would have 20 conditions, and so on.

In principle, factorial designs can include any number of independent variables with any number of levels. For example, an experiment could include the type of psychotherapy (cognitive vs. behavioural), the length of the psychotherapy (2 weeks vs. 2 months), and the sex of the psychotherapist (female vs. male). This would be a 2 × 2 × 2 factorial design and would have eight conditions. Figure 8.2 shows one way to represent this design. In practice, it is unusual for there to be more than three independent variables with more than two or three levels each. This is for at least two reasons: For one, the number of conditions can quickly become unmanageable. For example, adding a fourth independent variable with three levels (e.g., therapist experience: low vs. medium vs. high) to the current example would make it a 2 × 2 × 2 × 3 factorial design with 24 distinct conditions. Second, the number of participants required to populate all of these conditions (while maintaining a reasonable ability to detect a real underlying effect) can render the design unfeasible (for more information, see the discussion about the importance of adequate statistical power in Chapter 13). As a result, in the remainder of this section we will focus on designs with two independent variables. The general principles discussed here extend in a straightforward way to more complex factorial designs.

Assigning Participants to Conditions

Recall that in a simple between-subjects design, each participant is tested in only one condition. In a simple within-subjects design, each participant is tested in all conditions. In a factorial experiment, the decision to take the between-subjects or within-subjects approach must be made separately for each independent variable. In a  between-subjects factorial design , all of the independent variables are manipulated between subjects. For example, all participants could be tested either while using a cell phone  or  while not using a cell phone and either during the day  or  during the night. This would mean that each participant was tested in one and only one condition. In a within-subjects factorial design, all of the independent variables are manipulated within subjects. All participants could be tested both while using a cell phone and  while not using a cell phone and both during the day  and  during the night. This would mean that each participant was tested in all conditions. The advantages and disadvantages of these two approaches are the same as those discussed in  Chapter 6 . The between-subjects design is conceptually simpler, avoids carryover effects, and minimizes the time and effort of each participant. The within-subjects design is more efficient for the researcher and controls extraneous participant variables.

It is also possible to manipulate one independent variable between subjects and another within subjects. This is called a  mixed factorial design . For example, a researcher might choose to treat cell phone use as a within-subjects factor by testing the same participants both while using a cell phone and while not using a cell phone (while counterbalancing the order of these two conditions). But he or she might choose to treat time of day as a between-subjects factor by testing each participant either during the day or during the night (perhaps because this only requires them to come in for testing once). Thus each participant in this mixed design would be tested in two of the four conditions.

Regardless of whether the design is between subjects, within subjects, or mixed, the actual assignment of participants to conditions or orders of conditions is typically done randomly.

Nonmanipulated Independent Variables

In many factorial designs, one of the independent variables is a nonmanipulated independent variable . The researcher measures it but does not manipulate it. The study by Schnall and colleagues is a good example. One independent variable was disgust, which the researchers manipulated by testing participants in a clean room or a messy room. The other was private body consciousness, a participant variable which the researchers simply measured. Another example is a study by Halle Brown and colleagues in which participants were exposed to several words that they were later asked to recall (Brown, Kosslyn, Delamater, Fama, & Barsky, 1999) [1] . The manipulated independent variable was the type of word. Some were negative health-related words (e.g.,  tumor, coronary ), and others were not health related (e.g.,  election, geometry ). The nonmanipulated independent variable was whether participants were high or low in hypochondriasis (excessive concern with ordinary bodily symptoms). The result of this study was that the participants high in hypochondriasis were better than those low in hypochondriasis at recalling the health-related words, but they were no better at recalling the non-health-related words.

Such studies are extremely common, and there are several points worth making about them. First, nonmanipulated independent variables are usually participant variables (private body consciousness, hypochondriasis, self-esteem, and so on), and as such they are by definition between-subjects factors. For example, people are either low in hypochondriasis or high in hypochondriasis; they cannot be tested in both of these conditions. Second, such studies are generally considered to be experiments as long as at least one independent variable is manipulated, regardless of how many nonmanipulated independent variables are included. Third, it is important to remember that causal conclusions can only be drawn about the manipulated independent variable. For example, Schnall and her colleagues were justified in concluding that disgust affected the harshness of their participants’ moral judgments because they manipulated that variable and randomly assigned participants to the clean or messy room. But they would not have been justified in concluding that participants’ private body consciousness affected the harshness of their participants’ moral judgments because they did not manipulate that variable. It could be, for example, that having a strict moral code and a heightened awareness of one’s body are both caused by some third variable (e.g., neuroticism). Thus it is important to be aware of which variables in a study are manipulated and which are not.

Graphing the Results of Factorial Experiments

The results of factorial experiments with two independent variables can be graphed by representing one independent variable on the  x -axis and representing the other by using different kinds of bars or lines. (The  y -axis is always reserved for the dependent variable.) Figure 8.3 shows results for two hypothetical factorial experiments. The top panel shows the results of a 2 × 2 design. Time of day (day vs. night) is represented by different locations on the  x -axis, and cell phone use (no vs. yes) is represented by different-coloured bars. (It would also be possible to represent cell phone use on the  x -axis and time of day as different-coloured bars. The choice comes down to which way seems to communicate the results most clearly.) The bottom panel of Figure 8.3 shows the results of a 4 × 2 design in which one of the variables is quantitative. This variable, psychotherapy length, is represented along the  x -axis, and the other variable (psychotherapy type) is represented by differently formatted lines. This is a line graph rather than a bar graph because the variable on the x-axis is quantitative with a small number of distinct levels. Line graphs are also appropriate when representing measurements made over a time interval (also referred to as time series information) on the x -axis.

Main Effects and Interactions

In factorial designs, there are two kinds of results that are of interest: main effects and interaction effects (which are also just called “interactions”). A main effect  is the statistical relationship between one independent variable and a dependent variable—averaging across the levels of the other independent variable. Thus there is one main effect to consider for each independent variable in the study. The top panel of Figure 8.3 shows a main effect of cell phone use because driving performance was better, on average, when participants were not using cell phones than when they were. The blue bars are, on average, higher than the red bars. It also shows a main effect of time of day because driving performance was better during the day than during the night—both when participants were using cell phones and when they were not. Main effects are independent of each other in the sense that whether or not there is a main effect of one independent variable says nothing about whether or not there is a main effect of the other. The bottom panel of Figure 8.3 , for example, shows a clear main effect of psychotherapy length. The longer the psychotherapy, the better it worked.

There is an  interaction  effect (or just “interaction”) when the effect of one independent variable depends on the level of another. Although this might seem complicated, you already have an intuitive understanding of interactions. It probably would not surprise you, for example, to hear that the effect of receiving psychotherapy is stronger among people who are highly motivated to change than among people who are not motivated to change. This is an interaction because the effect of one independent variable (whether or not one receives psychotherapy) depends on the level of another (motivation to change). Schnall and her colleagues also demonstrated an interaction because the effect of whether the room was clean or messy on participants’ moral judgments depended on whether the participants were low or high in private body consciousness. If they were high in private body consciousness, then those in the messy room made harsher judgments. If they were low in private body consciousness, then whether the room was clean or messy did not matter.

The effect of one independent variable can depend on the level of the other in several different ways. This is shown in Figure 8.4 . In the top panel, independent variable “B” has an effect at level 1 of independent variable “A” but no effect at level 2 of independent variable “A.” (This is much like the study of Schnall and her colleagues where there was an effect of disgust for those high in private body consciousness but not for those low in private body consciousness.) In the middle panel, independent variable “B” has a stronger effect at level 1 of independent variable “A” than at level 2. This is like the hypothetical driving example where there was a stronger effect of using a cell phone at night than during the day. In the bottom panel, independent variable “B” again has an effect at both levels of independent variable “A,” but the effects are in opposite directions. Figure 8.4 shows the strongest form of this kind of interaction, called a crossover interaction. One example of a crossover interaction comes from a study by Kathy Gilliland on the effect of caffeine on the verbal test scores of introverts and extraverts (Gilliland, 1980) [2] . Introverts perform better than extraverts when they have not ingested any caffeine. But extraverts perform better than introverts when they have ingested 4 mg of caffeine per kilogram of body weight.

Figure 8.5 shows examples of these same kinds of interactions when one of the independent variables is quantitative and the results are plotted in a line graph. Note that in a crossover interaction, the two lines literally “cross over” each other.

In many studies, the primary research question is about an interaction. The study by Brown and her colleagues was inspired by the idea that people with hypochondriasis are especially attentive to any negative health-related information. This led to the hypothesis that people high in hypochondriasis would recall negative health-related words more accurately than people low in hypochondriasis but recall non-health-related words about the same as people low in hypochondriasis. And of course this is exactly what happened in this study.

Key Takeaways

• Researchers often include multiple independent variables in their experiments. The most common approach is the factorial design, in which each level of one independent variable is combined with each level of the others to create all possible conditions.
• In a factorial design, the main effect of an independent variable is its overall effect averaged across all other independent variables. There is one main effect for each independent variable.
• There is an interaction between two independent variables when the effect of one depends on the level of the other. Some of the most interesting research questions and results in psychology are specifically about interactions.
• Practice: Return to the five article titles presented at the beginning of this section. For each one, identify the independent variables and the dependent variable.
• Practice: Create a factorial design table for an experiment on the effects of room temperature and noise level on performance on the MCAT. Be sure to indicate whether each independent variable will be manipulated between-subjects or within-subjects and explain why.
• No main effect of A; no main effect of B; no interaction
• Main effect of A; no main effect of B; no interaction
• No main effect of A; main effect of B; no interaction
• Main effect of A; main effect of B; no interaction
• Main effect of A; main effect of B; interaction
• Main effect of A; no main effect of B; interaction
• No main effect of A; main effect of B; interaction
• No main effect of A; no main effect of B; interaction

Image Descriptions

Figure 8.5 image description: Three panels, each showing a different line graph pattern. In the top panel, one line remains constant while the other goes up. In the middle panel, both lines go up but at different rates. In the bottom panel, one line goes down and the other goes up so they cross. [Return to Figure 8.5]

• Brown, H. D., Kosslyn, S. M., Delamater, B., Fama, A., & Barsky, A. J. (1999). Perceptual and memory biases for health-related information in hypochondriacal individuals. Journal of Psychosomatic Research, 47 , 67–78. ↵
• Gilliland, K. (1980). The interactive effect of introversion-extroversion with caffeine induced arousal on verbal performance. Journal of Research in Personality, 14 , 482–492. ↵

An approach to including multiple independent variables in an experiment where each level of one independent variable is combined with each level of the others to produce all possible combinations.

A table showing each condition produced by the combinations of variables.

All of the independent variables are manipulated between subjects.

When one independent variable is manipulated between subjects and another is manipulated within subjects.

In a factorial design, the researcher measures an independent variable but does not manipulate it.

In factorial design, the statistical relationship between one independent variable and a dependent variable--averaging across the levels of the other independent variable.

When the effect of one independent variable depends on the level of another.

Research Methods in Psychology - 2nd Canadian Edition Copyright © 2015 by Paul C. Price, Rajiv Jhangiani, & I-Chant A. Chiang is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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Research Hypothesis In Psychology: Types, & Examples

Saul McLeod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul McLeod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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On This Page:

A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

• A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
• It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
• A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
• Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
• For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
• Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.

Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

• Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.

• Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
• However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

• Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
• Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
• Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
• Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
• Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

• The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
• The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

• Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
• Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
• Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
• Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
• Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
• Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
• Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
• Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Educational resources and simple solutions for your research journey

What is a Research Hypothesis: How to Write it, Types, and Examples

Any research begins with a research question and a research hypothesis . A research question alone may not suffice to design the experiment(s) needed to answer it. A hypothesis is central to the scientific method. But what is a hypothesis ? A hypothesis is a testable statement that proposes a possible explanation to a phenomenon, and it may include a prediction. Next, you may ask what is a research hypothesis ? Simply put, a research hypothesis is a prediction or educated guess about the relationship between the variables that you want to investigate.  

It is important to be thorough when developing your research hypothesis. Shortcomings in the framing of a hypothesis can affect the study design and the results. A better understanding of the research hypothesis definition and characteristics of a good hypothesis will make it easier for you to develop your own hypothesis for your research. Let’s dive in to know more about the types of research hypothesis , how to write a research hypothesis , and some research hypothesis examples .  

Table of Contents

What is a hypothesis ?  

A hypothesis is based on the existing body of knowledge in a study area. Framed before the data are collected, a hypothesis states the tentative relationship between independent and dependent variables, along with a prediction of the outcome.  

What is a research hypothesis ?  

Young researchers starting out their journey are usually brimming with questions like “ What is a hypothesis ?” “ What is a research hypothesis ?” “How can I write a good research hypothesis ?”   

A research hypothesis is a statement that proposes a possible explanation for an observable phenomenon or pattern. It guides the direction of a study and predicts the outcome of the investigation. A research hypothesis is testable, i.e., it can be supported or disproven through experimentation or observation.     

Characteristics of a good hypothesis  

Here are the characteristics of a good hypothesis :  

• Clearly formulated and free of language errors and ambiguity  
• Concise and not unnecessarily verbose  
• Has clearly defined variables  
• Testable and stated in a way that allows for it to be disproven  
• Can be tested using a research design that is feasible, ethical, and practical   
• Specific and relevant to the research problem  
• Rooted in a thorough literature search  
• Can generate new knowledge or understanding.  

How to create an effective research hypothesis  

A study begins with the formulation of a research question. A researcher then performs background research. This background information forms the basis for building a good research hypothesis . The researcher then performs experiments, collects, and analyzes the data, interprets the findings, and ultimately, determines if the findings support or negate the original hypothesis.  

Let’s look at each step for creating an effective, testable, and good research hypothesis :  

• Identify a research problem or question: Start by identifying a specific research problem.   
• Review the literature: Conduct an in-depth review of the existing literature related to the research problem to grasp the current knowledge and gaps in the field.   
• Formulate a clear and testable hypothesis : Based on the research question, use existing knowledge to form a clear and testable hypothesis . The hypothesis should state a predicted relationship between two or more variables that can be measured and manipulated. Improve the original draft till it is clear and meaningful.  
• State the null hypothesis: The null hypothesis is a statement that there is no relationship between the variables you are studying.   
• Define the population and sample: Clearly define the population you are studying and the sample you will be using for your research.  
• Select appropriate methods for testing the hypothesis: Select appropriate research methods, such as experiments, surveys, or observational studies, which will allow you to test your research hypothesis .  

Remember that creating a research hypothesis is an iterative process, i.e., you might have to revise it based on the data you collect. You may need to test and reject several hypotheses before answering the research problem.  

How to write a research hypothesis  

When you start writing a research hypothesis , you use an “if–then” statement format, which states the predicted relationship between two or more variables. Clearly identify the independent variables (the variables being changed) and the dependent variables (the variables being measured), as well as the population you are studying. Review and revise your hypothesis as needed.  

An example of a research hypothesis in this format is as follows:  

“ If [athletes] follow [cold water showers daily], then their [endurance] increases.”  

Population: athletes  

Independent variable: daily cold water showers  

Dependent variable: endurance  

You may have understood the characteristics of a good hypothesis . But note that a research hypothesis is not always confirmed; a researcher should be prepared to accept or reject the hypothesis based on the study findings.  

Research hypothesis checklist  

Following from above, here is a 10-point checklist for a good research hypothesis :  

• Testable: A research hypothesis should be able to be tested via experimentation or observation.  
• Specific: A research hypothesis should clearly state the relationship between the variables being studied.  
• Based on prior research: A research hypothesis should be based on existing knowledge and previous research in the field.  
• Falsifiable: A research hypothesis should be able to be disproven through testing.  
• Clear and concise: A research hypothesis should be stated in a clear and concise manner.  
• Logical: A research hypothesis should be logical and consistent with current understanding of the subject.  
• Relevant: A research hypothesis should be relevant to the research question and objectives.  
• Feasible: A research hypothesis should be feasible to test within the scope of the study.  
• Reflects the population: A research hypothesis should consider the population or sample being studied.  
• Uncomplicated: A good research hypothesis is written in a way that is easy for the target audience to understand.  

By following this research hypothesis checklist , you will be able to create a research hypothesis that is strong, well-constructed, and more likely to yield meaningful results.  

Types of research hypothesis  

Different types of research hypothesis are used in scientific research:  

1. Null hypothesis:

A null hypothesis states that there is no change in the dependent variable due to changes to the independent variable. This means that the results are due to chance and are not significant. A null hypothesis is denoted as H0 and is stated as the opposite of what the alternative hypothesis states.   

Example: “ The newly identified virus is not zoonotic .”  

2. Alternative hypothesis:

This states that there is a significant difference or relationship between the variables being studied. It is denoted as H1 or Ha and is usually accepted or rejected in favor of the null hypothesis.  

Example: “ The newly identified virus is zoonotic .”  

3. Directional hypothesis :

This specifies the direction of the relationship or difference between variables; therefore, it tends to use terms like increase, decrease, positive, negative, more, or less.   

Example: “ The inclusion of intervention X decreases infant mortality compared to the original treatment .”   

4. Non-directional hypothesis:

While it does not predict the exact direction or nature of the relationship between the two variables, a non-directional hypothesis states the existence of a relationship or difference between variables but not the direction, nature, or magnitude of the relationship. A non-directional hypothesis may be used when there is no underlying theory or when findings contradict previous research.  

Example, “ Cats and dogs differ in the amount of affection they express .”  

5. Simple hypothesis :

A simple hypothesis only predicts the relationship between one independent and another independent variable.  

Example: “ Applying sunscreen every day slows skin aging .”  

6 . Complex hypothesis :

A complex hypothesis states the relationship or difference between two or more independent and dependent variables.   

Example: “ Applying sunscreen every day slows skin aging, reduces sun burn, and reduces the chances of skin cancer .” (Here, the three dependent variables are slowing skin aging, reducing sun burn, and reducing the chances of skin cancer.)  

7. Associative hypothesis:  

An associative hypothesis states that a change in one variable results in the change of the other variable. The associative hypothesis defines interdependency between variables.  

Example: “ There is a positive association between physical activity levels and overall health .”  

8 . Causal hypothesis:

A causal hypothesis proposes a cause-and-effect interaction between variables.  

Example: “ Long-term alcohol use causes liver damage .”  

Note that some of the types of research hypothesis mentioned above might overlap. The types of hypothesis chosen will depend on the research question and the objective of the study.  

Research hypothesis examples  

Here are some good research hypothesis examples :  

“The use of a specific type of therapy will lead to a reduction in symptoms of depression in individuals with a history of major depressive disorder.”  

“Providing educational interventions on healthy eating habits will result in weight loss in overweight individuals.”  

“Plants that are exposed to certain types of music will grow taller than those that are not exposed to music.”  

“The use of the plant growth regulator X will lead to an increase in the number of flowers produced by plants.”  

Characteristics that make a research hypothesis weak are unclear variables, unoriginality, being too general or too vague, and being untestable. A weak hypothesis leads to weak research and improper methods.   

Some bad research hypothesis examples (and the reasons why they are “bad”) are as follows:  

“This study will show that treatment X is better than any other treatment . ” (This statement is not testable, too broad, and does not consider other treatments that may be effective.)  

“This study will prove that this type of therapy is effective for all mental disorders . ” (This statement is too broad and not testable as mental disorders are complex and different disorders may respond differently to different types of therapy.)  

“Plants can communicate with each other through telepathy . ” (This statement is not testable and lacks a scientific basis.)  

Importance of testable hypothesis  

If a research hypothesis is not testable, the results will not prove or disprove anything meaningful. The conclusions will be vague at best. A testable hypothesis helps a researcher focus on the study outcome and understand the implication of the question and the different variables involved. A testable hypothesis helps a researcher make precise predictions based on prior research.  

To be considered testable, there must be a way to prove that the hypothesis is true or false; further, the results of the hypothesis must be reproducible.  

Frequently Asked Questions (FAQs) on research hypothesis  

1. What is the difference between research question and research hypothesis ?  

A research question defines the problem and helps outline the study objective(s). It is an open-ended statement that is exploratory or probing in nature. Therefore, it does not make predictions or assumptions. It helps a researcher identify what information to collect. A research hypothesis , however, is a specific, testable prediction about the relationship between variables. Accordingly, it guides the study design and data analysis approach.

2. When to reject null hypothesis ?

A null hypothesis should be rejected when the evidence from a statistical test shows that it is unlikely to be true. This happens when the test statistic (e.g., p -value) is less than the defined significance level (e.g., 0.05). Rejecting the null hypothesis does not necessarily mean that the alternative hypothesis is true; it simply means that the evidence found is not compatible with the null hypothesis.  

3. How can I be sure my hypothesis is testable?  

A testable hypothesis should be specific and measurable, and it should state a clear relationship between variables that can be tested with data. To ensure that your hypothesis is testable, consider the following:  

• Clearly define the key variables in your hypothesis. You should be able to measure and manipulate these variables in a way that allows you to test the hypothesis.  
• The hypothesis should predict a specific outcome or relationship between variables that can be measured or quantified.   
• You should be able to collect the necessary data within the constraints of your study.  
• It should be possible for other researchers to replicate your study, using the same methods and variables.   
• Your hypothesis should be testable by using appropriate statistical analysis techniques, so you can draw conclusions, and make inferences about the population from the sample data.  
• The hypothesis should be able to be disproven or rejected through the collection of data.  

4. How do I revise my research hypothesis if my data does not support it?  

If your data does not support your research hypothesis , you will need to revise it or develop a new one. You should examine your data carefully and identify any patterns or anomalies, re-examine your research question, and/or revisit your theory to look for any alternative explanations for your results. Based on your review of the data, literature, and theories, modify your research hypothesis to better align it with the results you obtained. Use your revised hypothesis to guide your research design and data collection. It is important to remain objective throughout the process.  

5. I am performing exploratory research. Do I need to formulate a research hypothesis?  

As opposed to “confirmatory” research, where a researcher has some idea about the relationship between the variables under investigation, exploratory research (or hypothesis-generating research) looks into a completely new topic about which limited information is available. Therefore, the researcher will not have any prior hypotheses. In such cases, a researcher will need to develop a post-hoc hypothesis. A post-hoc research hypothesis is generated after these results are known.  

6. How is a research hypothesis different from a research question?

A research question is an inquiry about a specific topic or phenomenon, typically expressed as a question. It seeks to explore and understand a particular aspect of the research subject. In contrast, a research hypothesis is a specific statement or prediction that suggests an expected relationship between variables. It is formulated based on existing knowledge or theories and guides the research design and data analysis.

7. Can a research hypothesis change during the research process?

Yes, research hypotheses can change during the research process. As researchers collect and analyze data, new insights and information may emerge that require modification or refinement of the initial hypotheses. This can be due to unexpected findings, limitations in the original hypotheses, or the need to explore additional dimensions of the research topic. Flexibility is crucial in research, allowing for adaptation and adjustment of hypotheses to align with the evolving understanding of the subject matter.

8. How many hypotheses should be included in a research study?

The number of research hypotheses in a research study varies depending on the nature and scope of the research. It is not necessary to have multiple hypotheses in every study. Some studies may have only one primary hypothesis, while others may have several related hypotheses. The number of hypotheses should be determined based on the research objectives, research questions, and the complexity of the research topic. It is important to ensure that the hypotheses are focused, testable, and directly related to the research aims.

9. Can research hypotheses be used in qualitative research?

Yes, research hypotheses can be used in qualitative research, although they are more commonly associated with quantitative research. In qualitative research, hypotheses may be formulated as tentative or exploratory statements that guide the investigation. Instead of testing hypotheses through statistical analysis, qualitative researchers may use the hypotheses to guide data collection and analysis, seeking to uncover patterns, themes, or relationships within the qualitative data. The emphasis in qualitative research is often on generating insights and understanding rather than confirming or rejecting specific research hypotheses through statistical testing.

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How to Write a Hypothesis in 6 Steps, With Examples

A hypothesis is a statement that explains the predictions and reasoning of your research—an “educated guess” about how your scientific experiments will end. As a fundamental part of the scientific method, a good hypothesis is carefully written, but even the simplest ones can be difficult to put into words. 

Want to know how to write a hypothesis for your academic paper ? Below we explain the different types of hypotheses, what a good hypothesis requires, the steps to write your own, and plenty of examples.

Write with confidence Grammarly helps you polish your academic writing Write with Grammarly  

What is a hypothesis? 

One of our 10 essential words for university success , a hypothesis is one of the earliest stages of the scientific method. It’s essentially an educated guess—based on observations—of what the results of your experiment or research will be. 

Some hypothesis examples include:

• If I water plants daily they will grow faster.
• Adults can more accurately guess the temperature than children can. 
• Butterflies prefer white flowers to orange ones.

If you’ve noticed that watering your plants every day makes them grow faster, your hypothesis might be “plants grow better with regular watering.” From there, you can begin experiments to test your hypothesis; in this example, you might set aside two plants, water one but not the other, and then record the results to see the differences. 

The language of hypotheses always discusses variables , or the elements that you’re testing. Variables can be objects, events, concepts, etc.—whatever is observable. 

There are two types of variables: independent and dependent. Independent variables are the ones that you change for your experiment, whereas dependent variables are the ones that you can only observe. In the above example, our independent variable is how often we water the plants and the dependent variable is how well they grow. 

Hypotheses determine the direction and organization of your subsequent research methods, and that makes them a big part of writing a research paper . Ultimately the reader wants to know whether your hypothesis was proven true or false, so it must be written clearly in the introduction and/or abstract of your paper. 

7 examples of hypotheses

Depending on the nature of your research and what you expect to find, your hypothesis will fall into one or more of the seven main categories. Keep in mind that these categories are not exclusive, so the same hypothesis might qualify as several different types. 

1 Simple hypothesis

A simple hypothesis suggests only the relationship between two variables: one independent and one dependent. 

• If you stay up late, then you feel tired the next day. 
• Turning off your phone makes it charge faster. 

2 Complex hypothesis

A complex hypothesis suggests the relationship between more than two variables, for example, two independents and one dependent, or vice versa. 

• People who both (1) eat a lot of fatty foods and (2) have a family history of health problems are more likely to develop heart diseases. 
• Older people who live in rural areas are happier than younger people who live in rural areas. 

3 Null hypothesis

A null hypothesis, abbreviated as H 0 , suggests that there is no relationship between variables. 

• There is no difference in plant growth when using either bottled water or tap water. 
• Professional psychics do not win the lottery more than other people. 

4 Alternative hypothesis

An alternative hypothesis, abbreviated as H 1 or H A , is used in conjunction with a null hypothesis. It states the opposite of the null hypothesis, so that one and only one must be true. 

• Plants grow better with bottled water than tap water. 
• Professional psychics win the lottery more than other people. 

5 Logical hypothesis

A logical hypothesis suggests a relationship between variables without actual evidence. Claims are instead based on reasoning or deduction, but lack actual data.  

• An alien raised on Venus would have trouble breathing in Earth’s atmosphere. 
• Dinosaurs with sharp, pointed teeth were probably carnivores. 

6 Empirical hypothesis

An empirical hypothesis, also known as a “working hypothesis,” is one that is currently being tested. Unlike logical hypotheses, empirical hypotheses rely on concrete data. 

• Customers at restaurants will tip the same even if the wait staff’s base salary is raised. 
• Washing your hands every hour can reduce the frequency of illness. 

7 Statistical hypothesis

A statistical hypothesis is when you test only a sample of a population and then apply statistical evidence to the results to draw a conclusion about the entire population. Instead of testing everything , you test only a portion and generalize the rest based on preexisting data. 

• In humans, the birth-gender ratio of males to females is 1.05 to 1.00.  
• Approximately 2% of the world population has natural red hair. 

What makes a good hypothesis?

No matter what you’re testing, a good hypothesis is written according to the same guidelines. In particular, keep these five characteristics in mind: 

Cause and effect

Hypotheses always include a cause-and-effect relationship where one variable causes another to change (or not change if you’re using a null hypothesis). This can best be reflected as an if-then statement: If one variable occurs, then another variable changes. 

Testable prediction

Most hypotheses are designed to be tested (with the exception of logical hypotheses). Before committing to a hypothesis, make sure you’re actually able to conduct experiments on it. Choose a testable hypothesis with an independent variable that you have absolute control over. 

Independent and dependent variables

Define your variables in your hypothesis so your readers understand the big picture. You don’t have to specifically say which ones are independent and dependent variables, but you definitely want to mention them all. 

Candid language

Writing can easily get convoluted, so make sure your hypothesis remains as simple and clear as possible. Readers use your hypothesis as a contextual pillar to unify your entire paper, so there should be no confusion or ambiguity. If you’re unsure about your phrasing, try reading your hypothesis to a friend to see if they understand. 

Adherence to ethics

It’s not always about what you can test, but what you should test. Avoid hypotheses that require questionable or taboo experiments to keep ethics (and therefore, credibility) intact.

How to write a hypothesis in 6 steps

1 ask a question.

Curiosity has inspired some of history’s greatest scientific achievements, so a good place to start is to ask yourself questions about the world around you. Why are things the way they are? What causes the factors you see around you? If you can, choose a research topic that you’re interested in so your curiosity comes naturally. 

2 Conduct preliminary research

Next, collect some background information on your topic. How much background information you need depends on what you’re attempting. It could require reading several books, or it could be as simple as performing a web search for a quick answer. You don’t necessarily have to prove or disprove your hypothesis at this stage; rather, collect only what you need to prove or disprove it yourself. 

3 Define your variables

Once you have an idea of what your hypothesis will be, select which variables are independent and which are dependent. Remember that independent variables can only be factors that you have absolute control over, so consider the limits of your experiment before finalizing your hypothesis. 

4 Phrase it as an if-then statement

When writing a hypothesis, it helps to phrase it using an if-then format, such as, “ If I water a plant every day, then it will grow better.” This format can get tricky when dealing with multiple variables, but in general, it’s a reliable method for expressing the cause-and-effect relationship you’re testing. 

5  Collect data to support your hypothesis

A hypothesis is merely a means to an end. The priority of any scientific research is the conclusion. Once you have your hypothesis laid out and your variables chosen, you can then begin your experiments. Ideally, you’ll collect data to support your hypothesis, but don’t worry if your research ends up proving it wrong—that’s all part of the scientific method. 

6 Write with confidence

Last, you’ll want to record your findings in a research paper for others to see. This requires a bit of writing know-how, quite a different skill set than conducting experiments. 

That’s where Grammarly can be a major help; our writing suggestions point out not only grammar and spelling mistakes , but also new word choices and better phrasing. While you write, Grammarly automatically recommends optimal language and highlights areas where readers might get confused, ensuring that your hypothesis—and your final paper—are clear and polished.

Research Variables 101

Independent variables, dependent variables, control variables and more

By: Derek Jansen (MBA) | Expert Reviewed By: Kerryn Warren (PhD) | January 2023

If you’re new to the world of research, especially scientific research, you’re bound to run into the concept of variables , sooner or later. If you’re feeling a little confused, don’t worry – you’re not the only one! Independent variables, dependent variables, confounding variables – it’s a lot of jargon. In this post, we’ll unpack the terminology surrounding research variables using straightforward language and loads of examples .

Overview: Variables In Research

What (exactly) is a variable?

The simplest way to understand a variable is as any characteristic or attribute that can experience change or vary over time or context – hence the name “variable”. For example, the dosage of a particular medicine could be classified as a variable, as the amount can vary (i.e., a higher dose or a lower dose). Similarly, gender, age or ethnicity could be considered demographic variables, because each person varies in these respects.

Within research, especially scientific research, variables form the foundation of studies, as researchers are often interested in how one variable impacts another, and the relationships between different variables. For example:

• How someone’s age impacts their sleep quality
• How different teaching methods impact learning outcomes
• How diet impacts weight (gain or loss)

As you can see, variables are often used to explain relationships between different elements and phenomena. In scientific studies, especially experimental studies, the objective is often to understand the causal relationships between variables. In other words, the role of cause and effect between variables. This is achieved by manipulating certain variables while controlling others – and then observing the outcome. But, we’ll get into that a little later…

The “Big 3” Variables

Variables can be a little intimidating for new researchers because there are a wide variety of variables, and oftentimes, there are multiple labels for the same thing. To lay a firm foundation, we’ll first look at the three main types of variables, namely:

• Independent variables (IV)
• Dependant variables (DV)
• Control variables

What is an independent variable?

Simply put, the independent variable is the “ cause ” in the relationship between two (or more) variables. In other words, when the independent variable changes, it has an impact on another variable.

For example:

• Increasing the dosage of a medication (Variable A) could result in better (or worse) health outcomes for a patient (Variable B)
• Changing a teaching method (Variable A) could impact the test scores that students earn in a standardised test (Variable B)
• Varying one’s diet (Variable A) could result in weight loss or gain (Variable B).

It’s useful to know that independent variables can go by a few different names, including, explanatory variables (because they explain an event or outcome) and predictor variables (because they predict the value of another variable). Terminology aside though, the most important takeaway is that independent variables are assumed to be the “cause” in any cause-effect relationship. As you can imagine, these types of variables are of major interest to researchers, as many studies seek to understand the causal factors behind a phenomenon.

Need a helping hand?

What is a dependent variable?

While the independent variable is the “ cause ”, the dependent variable is the “ effect ” – or rather, the affected variable . In other words, the dependent variable is the variable that is assumed to change as a result of a change in the independent variable.

Keeping with the previous example, let’s look at some dependent variables in action:

• Health outcomes (DV) could be impacted by dosage changes of a medication (IV)
• Students’ scores (DV) could be impacted by teaching methods (IV)
• Weight gain or loss (DV) could be impacted by diet (IV)

In scientific studies, researchers will typically pay very close attention to the dependent variable (or variables), carefully measuring any changes in response to hypothesised independent variables. This can be tricky in practice, as it’s not always easy to reliably measure specific phenomena or outcomes – or to be certain that the actual cause of the change is in fact the independent variable.

As the adage goes, correlation is not causation . In other words, just because two variables have a relationship doesn’t mean that it’s a causal relationship – they may just happen to vary together. For example, you could find a correlation between the number of people who own a certain brand of car and the number of people who have a certain type of job. Just because the number of people who own that brand of car and the number of people who have that type of job is correlated, it doesn’t mean that owning that brand of car causes someone to have that type of job or vice versa. The correlation could, for example, be caused by another factor such as income level or age group, which would affect both car ownership and job type.

To confidently establish a causal relationship between an independent variable and a dependent variable (i.e., X causes Y), you’ll typically need an experimental design , where you have complete control over the environmen t and the variables of interest. But even so, this doesn’t always translate into the “real world”. Simply put, what happens in the lab sometimes stays in the lab!

As an alternative to pure experimental research, correlational or “ quasi-experimental ” research (where the researcher cannot manipulate or change variables) can be done on a much larger scale more easily, allowing one to understand specific relationships in the real world. These types of studies also assume some causality between independent and dependent variables, but it’s not always clear. So, if you go this route, you need to be cautious in terms of how you describe the impact and causality between variables and be sure to acknowledge any limitations in your own research.

What is a control variable?

In an experimental design, a control variable (or controlled variable) is a variable that is intentionally held constant to ensure it doesn’t have an influence on any other variables. As a result, this variable remains unchanged throughout the course of the study. In other words, it’s a variable that’s not allowed to vary – tough life 🙂

As we mentioned earlier, one of the major challenges in identifying and measuring causal relationships is that it’s difficult to isolate the impact of variables other than the independent variable. Simply put, there’s always a risk that there are factors beyond the ones you’re specifically looking at that might be impacting the results of your study. So, to minimise the risk of this, researchers will attempt (as best possible) to hold other variables constant . These factors are then considered control variables.

Some examples of variables that you may need to control include:

• Temperature
• Time of day
• Noise or distractions

Which specific variables need to be controlled for will vary tremendously depending on the research project at hand, so there’s no generic list of control variables to consult. As a researcher, you’ll need to think carefully about all the factors that could vary within your research context and then consider how you’ll go about controlling them. A good starting point is to look at previous studies similar to yours and pay close attention to which variables they controlled for.

Of course, you won’t always be able to control every possible variable, and so, in many cases, you’ll just have to acknowledge their potential impact and account for them in the conclusions you draw. Every study has its limitations , so don’t get fixated or discouraged by troublesome variables. Nevertheless, always think carefully about the factors beyond what you’re focusing on – don’t make assumptions!

Other types of variables

As we mentioned, independent, dependent and control variables are the most common variables you’ll come across in your research, but they’re certainly not the only ones you need to be aware of. Next, we’ll look at a few “secondary” variables that you need to keep in mind as you design your research.

• Moderating variables
• Mediating variables
• Confounding variables
• Latent variables

Let’s jump into it…

What is a moderating variable?

A moderating variable is a variable that influences the strength or direction of the relationship between an independent variable and a dependent variable. In other words, moderating variables affect how much (or how little) the IV affects the DV, or whether the IV has a positive or negative relationship with the DV (i.e., moves in the same or opposite direction).

For example, in a study about the effects of sleep deprivation on academic performance, gender could be used as a moderating variable to see if there are any differences in how men and women respond to a lack of sleep. In such a case, one may find that gender has an influence on how much students’ scores suffer when they’re deprived of sleep.

It’s important to note that while moderators can have an influence on outcomes , they don’t necessarily cause them ; rather they modify or “moderate” existing relationships between other variables. This means that it’s possible for two different groups with similar characteristics, but different levels of moderation, to experience very different results from the same experiment or study design.

What is a mediating variable?

Mediating variables are often used to explain the relationship between the independent and dependent variable (s). For example, if you were researching the effects of age on job satisfaction, then education level could be considered a mediating variable, as it may explain why older people have higher job satisfaction than younger people – they may have more experience or better qualifications, which lead to greater job satisfaction.

Mediating variables also help researchers understand how different factors interact with each other to influence outcomes. For instance, if you wanted to study the effect of stress on academic performance, then coping strategies might act as a mediating factor by influencing both stress levels and academic performance simultaneously. For example, students who use effective coping strategies might be less stressed but also perform better academically due to their improved mental state.

In addition, mediating variables can provide insight into causal relationships between two variables by helping researchers determine whether changes in one factor directly cause changes in another – or whether there is an indirect relationship between them mediated by some third factor(s). For instance, if you wanted to investigate the impact of parental involvement on student achievement, you would need to consider family dynamics as a potential mediator, since it could influence both parental involvement and student achievement simultaneously.

What is a confounding variable?

A confounding variable (also known as a third variable or lurking variable ) is an extraneous factor that can influence the relationship between two variables being studied. Specifically, for a variable to be considered a confounding variable, it needs to meet two criteria:

• It must be correlated with the independent variable (this can be causal or not)
• It must have a causal impact on the dependent variable (i.e., influence the DV)

Some common examples of confounding variables include demographic factors such as gender, ethnicity, socioeconomic status, age, education level, and health status. In addition to these, there are also environmental factors to consider. For example, air pollution could confound the impact of the variables of interest in a study investigating health outcomes.

Naturally, it’s important to identify as many confounding variables as possible when conducting your research, as they can heavily distort the results and lead you to draw incorrect conclusions . So, always think carefully about what factors may have a confounding effect on your variables of interest and try to manage these as best you can.

What is a latent variable?

Latent variables are unobservable factors that can influence the behaviour of individuals and explain certain outcomes within a study. They’re also known as hidden or underlying variables , and what makes them rather tricky is that they can’t be directly observed or measured . Instead, latent variables must be inferred from other observable data points such as responses to surveys or experiments.

For example, in a study of mental health, the variable “resilience” could be considered a latent variable. It can’t be directly measured , but it can be inferred from measures of mental health symptoms, stress, and coping mechanisms. The same applies to a lot of concepts we encounter every day – for example:

• Emotional intelligence
• Quality of life
• Business confidence
• Ease of use

One way in which we overcome the challenge of measuring the immeasurable is latent variable models (LVMs). An LVM is a type of statistical model that describes a relationship between observed variables and one or more unobserved (latent) variables. These models allow researchers to uncover patterns in their data which may not have been visible before, thanks to their complexity and interrelatedness with other variables. Those patterns can then inform hypotheses about cause-and-effect relationships among those same variables which were previously unknown prior to running the LVM. Powerful stuff, we say!

Let’s recap

In the world of scientific research, there’s no shortage of variable types, some of which have multiple names and some of which overlap with each other. In this post, we’ve covered some of the popular ones, but remember that this is not an exhaustive list .

To recap, we’ve explored:

• Independent variables (the “cause”)
• Dependent variables (the “effect”)
• Control variables (the variable that’s not allowed to vary)

If you’re still feeling a bit lost and need a helping hand with your research project, check out our 1-on-1 coaching service , where we guide you through each step of the research journey. Also, be sure to check out our free dissertation writing course and our collection of free, fully-editable chapter templates .

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Hypothesis Testing - Chi Squared Test

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Tests for Two or More Independent Samples, Discrete Outcome

Chi-squared tests in r.

Chi-Squared Table

All Modules

Here we extend that application of the chi-square test to the case with two or more independent comparison groups. Specifically, the outcome of interest is discrete with two or more responses and the responses can be ordered or unordered (i.e., the outcome can be dichotomous, ordinal or categorical). We now consider the situation where there are two or more independent comparison groups and the goal of the analysis is to compare the distribution of responses to the discrete outcome variable among several independent comparison groups.  

The test is called the χ 2 test of independence and the null hypothesis is that there is no difference in the distribution of responses to the outcome across comparison groups. This is often stated as follows: The outcome variable and the grouping variable (e.g., the comparison treatments or comparison groups) are independent (hence the name of the test). Independence here implies homogeneity in the distribution of the outcome among comparison groups.    

The null hypothesis in the χ 2 test of independence is often stated in words as: H 0 : The distribution of the outcome is independent of the groups. The alternative or research hypothesis is that there is a difference in the distribution of responses to the outcome variable among the comparison groups (i.e., that the distribution of responses "depends" on the group). In order to test the hypothesis, we measure the discrete outcome variable in each participant in each comparison group. The data of interest are the observed frequencies (or number of participants in each response category in each group). The formula for the test statistic for the χ 2 test of independence is given below.

Test Statistic for Testing H 0 : Distribution of outcome is independent of groups

and we find the critical value in a table of probabilities for the chi-square distribution with df=(r-1)*(c-1).

Here O = observed frequency, E=expected frequency in each of the response categories in each group, r = the number of rows in the two-way table and c = the number of columns in the two-way table.   r and c correspond to the number of comparison groups and the number of response options in the outcome (see below for more details). The observed frequencies are the sample data and the expected frequencies are computed as described below. The test statistic is appropriate for large samples, defined as expected frequencies of at least 5 in each of the response categories in each group.  

The data for the χ 2 test of independence are organized in a two-way table. The outcome and grouping variable are shown in the rows and columns of the table. The sample table below illustrates the data layout. The table entries (blank below) are the numbers of participants in each group responding to each response category of the outcome variable.

Table - Possible outcomes are are listed in the columns; The groups being compared are listed in rows.

In the table above, the grouping variable is shown in the rows of the table; r denotes the number of independent groups. The outcome variable is shown in the columns of the table; c denotes the number of response options in the outcome variable. Each combination of a row (group) and column (response) is called a cell of the table. The table has r*c cells and is sometimes called an r x c ("r by c") table. For example, if there are 4 groups and 5 categories in the outcome variable, the data are organized in a 4 X 5 table. The row and column totals are shown along the right-hand margin and the bottom of the table, respectively. The total sample size, N, can be computed by summing the row totals or the column totals. Similar to ANOVA, N does not refer to a population size here but rather to the total sample size in the analysis. The sample data can be organized into a table like the above. The numbers of participants within each group who select each response option are shown in the cells of the table and these are the observed frequencies used in the test statistic.

The test statistic for the χ 2 test of independence involves comparing observed (sample data) and expected frequencies in each cell of the table. The expected frequencies are computed assuming that the null hypothesis is true. The null hypothesis states that the two variables (the grouping variable and the outcome) are independent. The definition of independence is as follows:

 Two events, A and B, are independent if P(A|B) = P(A), or equivalently, if P(A and B) = P(A) P(B).

The second statement indicates that if two events, A and B, are independent then the probability of their intersection can be computed by multiplying the probability of each individual event. To conduct the χ 2 test of independence, we need to compute expected frequencies in each cell of the table. Expected frequencies are computed by assuming that the grouping variable and outcome are independent (i.e., under the null hypothesis). Thus, if the null hypothesis is true, using the definition of independence:

P(Group 1 and Response Option 1) = P(Group 1) P(Response Option 1).

 The above states that the probability that an individual is in Group 1 and their outcome is Response Option 1 is computed by multiplying the probability that person is in Group 1 by the probability that a person is in Response Option 1. To conduct the χ 2 test of independence, we need expected frequencies and not expected probabilities . To convert the above probability to a frequency, we multiply by N. Consider the following small example.

The data shown above are measured in a sample of size N=150. The frequencies in the cells of the table are the observed frequencies. If Group and Response are independent, then we can compute the probability that a person in the sample is in Group 1 and Response category 1 using:

P(Group 1 and Response 1) = P(Group 1) P(Response 1),

P(Group 1 and Response 1) = (25/150) (62/150) = 0.069.

Thus if Group and Response are independent we would expect 6.9% of the sample to be in the top left cell of the table (Group 1 and Response 1). The expected frequency is 150(0.069) = 10.4.   We could do the same for Group 2 and Response 1:

P(Group 2 and Response 1) = P(Group 2) P(Response 1),

P(Group 2 and Response 1) = (50/150) (62/150) = 0.138.

The expected frequency in Group 2 and Response 1 is 150(0.138) = 20.7.

Thus, the formula for determining the expected cell frequencies in the χ 2 test of independence is as follows:

Expected Cell Frequency = (Row Total * Column Total)/N.

The above computes the expected frequency in one step rather than computing the expected probability first and then converting to a frequency.  

In a prior example we evaluated data from a survey of university graduates which assessed, among other things, how frequently they exercised. The survey was completed by 470 graduates. In the prior example we used the χ 2 goodness-of-fit test to assess whether there was a shift in the distribution of responses to the exercise question following the implementation of a health promotion campaign on campus. We specifically considered one sample (all students) and compared the observed distribution to the distribution of responses the prior year (a historical control). Suppose we now wish to assess whether there is a relationship between exercise on campus and students' living arrangements. As part of the same survey, graduates were asked where they lived their senior year. The response options were dormitory, on-campus apartment, off-campus apartment, and at home (i.e., commuted to and from the university). The data are shown below.

Based on the data, is there a relationship between exercise and student's living arrangement? Do you think where a person lives affect their exercise status? Here we have four independent comparison groups (living arrangement) and a discrete (ordinal) outcome variable with three response options. We specifically want to test whether living arrangement and exercise are independent. We will run the test using the five-step approach.  

• Step 1. Set up hypotheses and determine level of significance.

H 0 : Living arrangement and exercise are independent

H 1 : H 0 is false.                α=0.05

The null and research hypotheses are written in words rather than in symbols. The research hypothesis is that the grouping variable (living arrangement) and the outcome variable (exercise) are dependent or related.   

• Step 2.  Select the appropriate test statistic.  

The formula for the test statistic is:

The condition for appropriate use of the above test statistic is that each expected frequency is at least 5. In Step 4 we will compute the expected frequencies and we will ensure that the condition is met.

• Step 3. Set up decision rule.  

The decision rule depends on the level of significance and the degrees of freedom, defined as df = (r-1)(c-1), where r and c are the numbers of rows and columns in the two-way data table.   The row variable is the living arrangement and there are 4 arrangements considered, thus r=4. The column variable is exercise and 3 responses are considered, thus c=3. For this test, df=(4-1)(3-1)=3(2)=6. Again, with χ 2 tests there are no upper, lower or two-tailed tests. If the null hypothesis is true, the observed and expected frequencies will be close in value and the χ 2 statistic will be close to zero. If the null hypothesis is false, then the χ 2 statistic will be large. The rejection region for the χ 2 test of independence is always in the upper (right-hand) tail of the distribution. For df=6 and a 5% level of significance, the appropriate critical value is 12.59 and the decision rule is as follows: Reject H 0 if c 2 > 12.59.

• Step 4. Compute the test statistic.  

We now compute the expected frequencies using the formula,

Expected Frequency = (Row Total * Column Total)/N.

The computations can be organized in a two-way table. The top number in each cell of the table is the observed frequency and the bottom number is the expected frequency.   The expected frequencies are shown in parentheses.

Notice that the expected frequencies are taken to one decimal place and that the sums of the observed frequencies are equal to the sums of the expected frequencies in each row and column of the table.  

Recall in Step 2 a condition for the appropriate use of the test statistic was that each expected frequency is at least 5. This is true for this sample (the smallest expected frequency is 9.6) and therefore it is appropriate to use the test statistic.

The test statistic is computed as follows:

• Step 5. Conclusion.  

We reject H 0 because 60.5 > 12.59. We have statistically significant evidence at a =0.05 to show that H 0 is false or that living arrangement and exercise are not independent (i.e., they are dependent or related), p < 0.005.  

Again, the χ 2 test of independence is used to test whether the distribution of the outcome variable is similar across the comparison groups. Here we rejected H 0 and concluded that the distribution of exercise is not independent of living arrangement, or that there is a relationship between living arrangement and exercise. The test provides an overall assessment of statistical significance. When the null hypothesis is rejected, it is important to review the sample data to understand the nature of the relationship. Consider again the sample data. 

Because there are different numbers of students in each living situation, it makes the comparisons of exercise patterns difficult on the basis of the frequencies alone. The following table displays the percentages of students in each exercise category by living arrangement. The percentages sum to 100% in each row of the table. For comparison purposes, percentages are also shown for the total sample along the bottom row of the table.

From the above, it is clear that higher percentages of students living in dormitories and in on-campus apartments reported regular exercise (31% and 23%) as compared to students living in off-campus apartments and at home (10% each).  

Test Yourself

( J Gastrointest Surgery, 2012, 16 275-281)', CAPTION, ' 

Question: What would be an appropriate statistical test to examine whether there is an association between Surgical Apgar Score and patient outcome? Using 14.13 as the value of the test statistic for these data, carry out the appropriate test at a 5% level of significance. Show all parts of your test.

In the module on hypothesis testing for means and proportions , we discussed hypothesis testing applications with a dichotomous outcome variable and two independent comparison groups. We presented a test using a test statistic Z to test for equality of independent proportions. The chi-square test of independence can also be used with a dichotomous outcome and the results are mathematically equivalent.  

In the prior module, we considered the following example. Here we show the equivalence to the chi-square test of independence.

A randomized trial is designed to evaluate the effectiveness of a newly developed pain reliever designed to reduce pain in patients following joint replacement surgery. The trial compares the new pain reliever to the pain reliever currently in use (called the standard of care). A total of 100 patients undergoing joint replacement surgery agreed to participate in the trial. Patients were randomly assigned to receive either the new pain reliever or the standard pain reliever following surgery and were blind to the treatment assignment. Before receiving the assigned treatment, patients were asked to rate their pain on a scale of 0-10 with higher scores indicative of more pain. Each patient was then given the assigned treatment and after 30 minutes was again asked to rate their pain on the same scale. The primary outcome was a reduction in pain of 3 or more scale points (defined by clinicians as a clinically meaningful reduction). The following data were observed in the trial.

We tested whether there was a significant difference in the proportions of patients reporting a meaningful reduction (i.e., a reduction of 3 or more scale points) using a Z statistic, as follows. 

• Step 1. Set up hypotheses and determine level of significance

H 0 : p 1 = p 2    

H 1 : p 1 ≠ p 2                             α=0.05

Here the new or experimental pain reliever is group 1 and the standard pain reliever is group 2.

• Step 2. Select the appropriate test statistic.  

We must first check that the sample size is adequate. Specifically, we need to ensure that we have at least 5 successes and 5 failures in each comparison group or that:

In this example, we have

Therefore, the sample size is adequate, so the following formula can be used:

Reject H 0 if Z < -1.960 or if Z > 1.960.

We now substitute the sample data into the formula for the test statistic identified in Step 2. We first compute the overall proportion of successes:

We now substitute to compute the test statistic.

• Step 5.  Conclusion.  

We now conduct the same test using the chi-square test of independence.  

H 0 : Treatment and outcome (meaningful reduction in pain) are independent

H 1 :   H 0 is false.         α=0.05

The formula for the test statistic is:  

For this test, df=(2-1)(2-1)=1. At a 5% level of significance, the appropriate critical value is 3.84 and the decision rule is as follows: Reject H0 if χ 2 > 3.84. (Note that 1.96 2 = 3.84, where 1.96 was the critical value used in the Z test for proportions shown above.)

We now compute the expected frequencies using:

The computations can be organized in a two-way table. The top number in each cell of the table is the observed frequency and the bottom number is the expected frequency. The expected frequencies are shown in parentheses.

A condition for the appropriate use of the test statistic was that each expected frequency is at least 5. This is true for this sample (the smallest expected frequency is 22.0) and therefore it is appropriate to use the test statistic.

(Note that (2.53) 2 = 6.4, where 2.53 was the value of the Z statistic in the test for proportions shown above.)

The video below by Mike Marin demonstrates how to perform chi-squared tests in the R programming language.

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Content ©2016. All Rights Reserved. Date last modified: September 1, 2016. Wayne W. LaMorte, MD, PhD, MPH

Independent Variables (Definition + 43 Examples)

Have you ever wondered how scientists make discoveries and how researchers come to understand the world around us? A crucial tool in their kit is the concept of the independent variable, which helps them delve into the mysteries of science and everyday life.

An independent variable is a condition or factor that researchers manipulate to observe its effect on another variable, known as the dependent variable. In simpler terms, it’s like adjusting the dials and watching what happens! By changing the independent variable, scientists can see if and how it causes changes in what they are measuring or observing, helping them make connections and draw conclusions.

In this article, we’ll explore the fascinating world of independent variables, journey through their history, examine theories, and look at a variety of examples from different fields.

History of the Independent Variable

Once upon a time, in a world thirsty for understanding, people observed the stars, the seas, and everything in between, seeking to unlock the mysteries of the universe.

The story of the independent variable begins with a quest for knowledge, a journey taken by thinkers and tinkerers who wanted to explain the wonders and strangeness of the world.

Origins of the Concept

The seeds of the idea of independent variables were sown by Sir Francis Galton , an English polymath, in the 19th century. Galton wore many hats—he was a psychologist, anthropologist, meteorologist, and a statistician!

It was his diverse interests that led him to explore the relationships between different factors and their effects. Galton was curious—how did one thing lead to another, and what could be learned from these connections?

As Galton delved into the world of statistical theories , the concept of independent variables started taking shape.

He was interested in understanding how characteristics, like height and intelligence, were passed down through generations.

Galton’s work laid the foundation for later thinkers to refine and expand the concept, turning it into an invaluable tool for scientific research.

Evolution over Time

After Galton’s pioneering work, the concept of the independent variable continued to evolve and grow. Scientists and researchers from various fields adopted and adapted it, finding new ways to use it to make sense of the world.

They discovered that by manipulating one factor (the independent variable), they could observe changes in another (the dependent variable), leading to groundbreaking insights and discoveries.

Through the years, the independent variable became a cornerstone in experimental design . Researchers in fields like physics, biology, psychology, and sociology used it to test hypotheses, develop theories, and uncover the laws that govern our universe.

The idea that originated from Galton’s curiosity had bloomed into a universal key, unlocking doors to knowledge across disciplines.

Importance in Scientific Research

Today, the independent variable stands tall as a pillar of scientific research. It helps scientists and researchers ask critical questions, test their ideas, and find answers. Without independent variables, we wouldn’t have many of the advancements and understandings that we take for granted today.

The independent variable plays a starring role in experiments, helping us learn about everything from the smallest particles to the vastness of space. It helps researchers create vaccines, understand social behaviors, explore ecological systems, and even develop new technologies.

In the upcoming sections, we’ll dive deeper into what independent variables are, how they work, and how they’re used in various fields.

Together, we’ll uncover the magic of this scientific concept and see how it continues to shape our understanding of the world around us.

What is an Independent Variable?

Embarking on the captivating journey of scientific exploration requires us to grasp the essential terms and ideas. It's akin to a treasure hunter mastering the use of a map and compass.

In our adventure through the realm of independent variables, we’ll delve deeper into some fundamental concepts and definitions to help us navigate this exciting world.

Variables in Research

In the grand tapestry of research, variables are the gems that researchers seek. They’re elements, characteristics, or behaviors that can shift or vary in different circumstances.

Picture them as the myriad of ingredients in a chef’s kitchen—each variable can be adjusted or modified to create a myriad of dishes, each with a unique flavor!

Understanding variables is essential as they form the core of every scientific experiment and observational study.

Types of Variables

Independent Variable The star of our story, the independent variable, is the one that researchers change or control to study its effects. It’s like a chef experimenting with different spices to see how each one alters the taste of the soup. The independent variable is the catalyst, the initial spark that sets the wheels of research in motion.

Dependent Variable The dependent variable is the outcome we observe and measure . It’s the altered flavor of the soup that results from the chef’s culinary experiments. This variable depends on the changes made to the independent variable, hence the name!

Observing how the dependent variable reacts to changes helps scientists draw conclusions and make discoveries.

Control Variable Control variables are the unsung heroes of scientific research. They’re the constants, the elements that researchers keep the same to ensure the integrity of the experiment.

Imagine if our chef used a different type of broth each time he experimented with spices—the results would be all over the place! Control variables keep the experiment grounded and help researchers be confident in their findings.

Confounding Variables Imagine a hidden rock in a stream, changing the water’s flow in unexpected ways. Confounding variables are similar—they are external factors that can sneak into experiments and influence the outcome , adding twists to our scientific story.

These variables can blur the relationship between the independent and dependent variables, making the results of the study a bit puzzly. Detecting and controlling these hidden elements helps researchers ensure the accuracy of their findings and reach true conclusions.

There are of course other types of variables, and different ways to manipulate them called " schedules of reinforcement ," but we won't get into that too much here.

Role of the Independent Variable

Manipulation When researchers manipulate the independent variable, they are orchestrating a symphony of cause and effect. They’re adjusting the strings, the brass, the percussion, observing how each change influences the melody—the dependent variable.

This manipulation is at the heart of experimental research. It allows scientists to explore relationships, unravel patterns, and unearth the secrets hidden within the fabric of our universe.

Observation With every tweak and adjustment made to the independent variable, researchers are like seasoned detectives, observing the dependent variable for changes, collecting clues, and piecing together the puzzle.

Observing the effects and changes that occur helps them deduce relationships, formulate theories, and expand our understanding of the world. Every observation is a step towards solving the mysteries of nature and human behavior.

Identifying Independent Variables

Characteristics Identifying an independent variable in the vast landscape of research can seem daunting, but fear not! Independent variables have distinctive characteristics that make them stand out.

They’re the elements that are deliberately changed or controlled in an experiment to study their effects on the dependent variable. Recognizing these characteristics is like learning to spot footprints in the sand—it leads us to the heart of the discovery!

In Different Types of Research The world of research is diverse and varied, and the independent variable dons many guises! In the field of medicine, it might manifest as the dosage of a drug administered to patients.

In psychology, it could take the form of different learning methods applied to study memory retention. In each field, identifying the independent variable correctly is the golden key that unlocks the treasure trove of knowledge and insights.

As we forge ahead on our enlightening journey, equipped with a deeper understanding of independent variables and their roles, we’re ready to delve into the intricate theories and diverse examples that underscore their significance.

Independent Variables in Research

Now that we’re acquainted with the basic concepts and have the tools to identify independent variables, let’s dive into the fascinating ocean of theories and frameworks.

These theories are like ancient scrolls, providing guidelines and blueprints that help scientists use independent variables to uncover the secrets of the universe.

Scientific Method

What is it and How Does it Work? The scientific method is like a super-helpful treasure map that scientists use to make discoveries. It has steps we follow: asking a question, researching, guessing what will happen (that's a hypothesis!), experimenting, checking the results, figuring out what they mean, and telling everyone about it.

Our hero, the independent variable, is the compass that helps this adventure go the right way!

How Independent Variables Lead the Way In the scientific method, the independent variable is like the captain of a ship, leading everyone through unknown waters.

Scientists change this variable to see what happens and to learn new things. It’s like having a compass that points us towards uncharted lands full of knowledge!

Experimental Design

The Basics of Building Constructing an experiment is like building a castle, and the independent variable is the cornerstone. It’s carefully chosen and manipulated to see how it affects the dependent variable. Researchers also identify control and confounding variables, ensuring the castle stands strong, and the results are reliable.

Keeping Everything in Check In every experiment, maintaining control is key to finding the treasure. Scientists use control variables to keep the conditions consistent, ensuring that any changes observed are truly due to the independent variable. It’s like ensuring the castle’s foundation is solid, supporting the structure as it reaches for the sky.

Hypothesis Testing

Making Educated Guesses Before they start experimenting, scientists make educated guesses called hypotheses . It’s like predicting which X marks the spot of the treasure! It often includes the independent variable and the expected effect on the dependent variable, guiding researchers as they navigate through the experiment.

Independent Variables in the Spotlight When testing these guesses, the independent variable is the star of the show! Scientists change and watch this variable to see if their guesses were right. It helps them figure out new stuff and learn more about the world around us!

Statistical Analysis

Figuring Out Relationships After the experimenting is done, it’s time for scientists to crack the code! They use statistics to understand how the independent and dependent variables are related and to uncover the hidden stories in the data.

Experimenters have to be careful about how they determine the validity of their findings, which is why they use statistics. Something called "experimenter bias" can get in the way of having true (valid) results, because it's basically when the experimenter influences the outcome based on what they believe to be true (or what they want to be true!).

How Important are the Discoveries? Through statistical analysis, scientists determine the significance of their findings. It’s like discovering if the treasure found is made of gold or just shiny rocks. The analysis helps researchers know if the independent variable truly had an effect, contributing to the rich tapestry of scientific knowledge.

As we uncover more about how theories and frameworks use independent variables, we start to see how awesome they are in helping us learn more about the world. But we’re not done yet!

Up next, we’ll look at tons of examples to see how independent variables work their magic in different areas.

Examples of Independent Variables

Independent variables take on many forms, showcasing their versatility in a range of experiments and studies. Let’s uncover how they act as the protagonists in numerous investigations and learning quests!

Science Experiments

1) plant growth.

Consider an experiment aiming to observe the effect of varying water amounts on plant height. In this scenario, the amount of water given to the plants is the independent variable!

2) Freezing Water

Suppose we are curious about the time it takes for water to freeze at different temperatures. The temperature of the freezer becomes the independent variable as we adjust it to observe the results!

3) Light and Shadow

Have you ever observed how shadows change? In an experiment, adjusting the light angle to observe its effect on an object’s shadow makes the angle of light the independent variable!

4) Medicine Dosage

In medical studies, determining how varying medicine dosages influence a patient’s recovery is essential. Here, the dosage of the medicine administered is the independent variable!

5) Exercise and Health

Researchers might examine the impact of different exercise forms on individuals’ health. The various exercise forms constitute the independent variable in this study!

6) Sleep and Wellness

Have you pondered how the sleep duration affects your well-being the following day? In such research, the hours of sleep serve as the independent variable!

7) Learning Methods

Psychologists might investigate how diverse study methods influence test outcomes. Here, the different study methods adopted by students are the independent variable!

8) Mood and Music

Have you experienced varied emotions with different music genres? The genre of music played becomes the independent variable when researching its influence on emotions!

9) Color and Feelings

Suppose researchers are exploring how room colors affect individuals’ emotions. In this case, the room colors act as the independent variable!

Environment

10) rainfall and plant life.

Environmental scientists may study the influence of varying rainfall levels on vegetation. In this instance, the amount of rainfall is the independent variable!

11) Temperature and Animal Behavior

Examining how temperature variations affect animal behavior is fascinating. Here, the varying temperatures serve as the independent variable!

12) Pollution and Air Quality

Investigating the effects of different pollution levels on air quality is crucial. In such studies, the pollution level is the independent variable!

13) Internet Speed and Productivity

Researchers might explore how varying internet speeds impact work productivity. In this exploration, the internet speed is the independent variable!

14) Device Type and User Experience

Examining how different devices affect user experience is interesting. Here, the type of device used is the independent variable!

15) Software Version and Performance

Suppose a study aims to determine how different software versions influence system performance. The software version becomes the independent variable!

16) Teaching Style and Student Engagement

Educators might investigate the effect of varied teaching styles on student engagement. In such a study, the teaching style is the independent variable!

17) Class Size and Learning Outcome

Researchers could explore how different class sizes influence students’ learning. Here, the class size is the independent variable!

18) Homework Frequency and Academic Achievement

Examining the relationship between the frequency of homework assignments and academic success is essential. The frequency of homework becomes the independent variable!

19) Telescope Type and Celestial Observation

Astronomers might study how different telescopes affect celestial observation. In this scenario, the telescope type is the independent variable!

20) Light Pollution and Star Visibility

Investigating the influence of varying light pollution levels on star visibility is intriguing. Here, the level of light pollution is the independent variable!

21) Observation Time and Astronomical Detail

Suppose a study explores how observation duration affects the detail captured in astronomical images. The duration of observation serves as the independent variable!

22) Community Size and Social Interaction

Sociologists may examine how the size of a community influences social interactions. In this research, the community size is the independent variable!

23) Cultural Exposure and Social Tolerance

Investigating the effect of diverse cultural exposure on social tolerance is vital. Here, the level of cultural exposure is the independent variable!

24) Economic Status and Educational Attainment

Researchers could explore how different economic statuses impact educational achievements. In such studies, economic status is the independent variable!

25) Training Intensity and Athletic Performance

Sports scientists might study how varying training intensities affect athletes’ performance. In this case, the training intensity is the independent variable!

26) Equipment Type and Player Safety

Examining the relationship between different sports equipment and player safety is crucial. Here, the type of equipment used is the independent variable!

27) Team Size and Game Strategy

Suppose researchers are investigating how the size of a sports team influences game strategy. The team size becomes the independent variable!

28) Diet Type and Health Outcome

Nutritionists may explore the impact of various diets on individuals’ health. In this exploration, the type of diet followed is the independent variable!

29) Caloric Intake and Weight Change

Investigating how different caloric intakes influence weight change is essential. In such a study, the caloric intake is the independent variable!

30) Food Variety and Nutrient Absorption

Researchers could examine how consuming a variety of foods affects nutrient absorption. Here, the variety of foods consumed is the independent variable!

Real-World Examples of Independent Variables

Isn't it fantastic how independent variables play such an essential part in so many studies? But the excitement doesn't stop there!

Now, let’s explore how findings from these studies, led by independent variables, make a big splash in the real world and improve our daily lives!

Healthcare Advancements

31) treatment optimization.

By studying different medicine dosages and treatment methods as independent variables, doctors can figure out the best ways to help patients recover quicker and feel better. This leads to more effective medicines and treatment plans!

32) Lifestyle Recommendations

Researching the effects of sleep, exercise, and diet helps health experts give us advice on living healthier lives. By changing these independent variables, scientists uncover the secrets to feeling good and staying well!

Technological Innovations

33) speeding up the internet.

When scientists explore how different internet speeds affect our online activities, they’re able to develop technologies to make the internet faster and more reliable. This means smoother video calls and quicker downloads!

34) Improving User Experience

By examining how we interact with various devices and software, researchers can design technology that’s easier and more enjoyable to use. This leads to cooler gadgets and more user-friendly apps!

Educational Strategies

35) enhancing learning.

Investigating different teaching styles, class sizes, and study methods helps educators discover what makes learning fun and effective. This research shapes classrooms, teaching methods, and even homework!

36) Tailoring Student Support

By studying how students with diverse needs respond to different support strategies, educators can create personalized learning experiences. This means every student gets the help they need to succeed!

Environmental Protection

37) conserving nature.

Researching how rainfall, temperature, and pollution affect the environment helps scientists suggest ways to protect our planet. By studying these independent variables, we learn how to keep nature healthy and thriving!

38) Combating Climate Change

Scientists studying the effects of pollution and human activities on climate change are leading the way in finding solutions. By exploring these independent variables, we can develop strategies to combat climate change and protect the Earth!

Social Development

39) building stronger communities.

Sociologists studying community size, cultural exposure, and economic status help us understand what makes communities happy and united. This knowledge guides the development of policies and programs for stronger societies!

40) Promoting Equality and Tolerance

By exploring how exposure to diverse cultures affects social tolerance, researchers contribute to fostering more inclusive and harmonious societies. This helps build a world where everyone is respected and valued!

Enhancing Sports Performance

41) optimizing athlete training.

Sports scientists studying training intensity, equipment type, and team size help athletes reach their full potential. This research leads to better training programs, safer equipment, and more exciting games!

42) Innovating Sports Strategies

By investigating how different game strategies are influenced by various team compositions, researchers contribute to the evolution of sports. This means more thrilling competitions and matches for us to enjoy!

Nutritional Well-Being

43) guiding healthy eating.

Nutritionists researching diet types, caloric intake, and food variety help us understand what foods are best for our bodies. This knowledge shapes dietary guidelines and helps us make tasty, yet nutritious, meal choices!

44) Promoting Nutritional Awareness

By studying the effects of different nutrients and diets, researchers educate us on maintaining a balanced diet. This fosters a greater awareness of nutritional well-being and encourages healthier eating habits!

As we journey through these real-world applications, we witness the incredible impact of studies featuring independent variables. The exploration doesn’t end here, though!

Let’s continue our adventure and see how we can identify independent variables in our own observations and inquiries! Keep your curiosity alive, and let’s delve deeper into the exciting realm of independent variables!

Identifying Independent Variables in Everyday Scenarios

So, we’ve seen how independent variables star in many studies, but how about spotting them in our everyday life?

Recognizing independent variables can be like a treasure hunt – you never know where you might find one! Let’s uncover some tips and tricks to identify these hidden gems in various situations.

1) Asking Questions

One of the best ways to spot an independent variable is by asking questions! If you’re curious about something, ask yourself, “What am I changing or manipulating in this situation?” The thing you’re changing is likely the independent variable!

For example, if you’re wondering whether the amount of sunlight affects how quickly your laundry dries, the sunlight amount is your independent variable!

2) Making Observations

Keep your eyes peeled and observe the world around you! By watching how changes in one thing (like the amount of rain) affect something else (like the height of grass), you can identify the independent variable.

In this case, the amount of rain is the independent variable because it’s what’s changing!

3) Conducting Experiments

Get hands-on and conduct your own experiments! By changing one thing and observing the results, you’re identifying the independent variable.

If you’re growing plants and decide to water each one differently to see the effects, the amount of water is your independent variable!

4) Everyday Scenarios

In everyday scenarios, independent variables are all around!

When you adjust the temperature of your oven to bake cookies, the oven temperature is the independent variable.

Or if you’re deciding how much time to spend studying for a test, the study time is your independent variable!

5) Being Curious

Keep being curious and asking “What if?” questions! By exploring different possibilities and wondering how changing one thing could affect another, you’re on your way to identifying independent variables.

If you’re curious about how the color of a room affects your mood, the room color is the independent variable!

6) Reviewing Past Studies

Don’t forget about the treasure trove of past studies and experiments! By reviewing what scientists and researchers have done before, you can learn how they identified independent variables in their work.

This can give you ideas and help you recognize independent variables in your own explorations!

Exercises for Identifying Independent Variables

Ready for some practice? Let’s put on our thinking caps and try to identify the independent variables in a few scenarios.

Remember, the independent variable is what’s being changed or manipulated to observe the effect on something else! (You can see the answers below)

Scenario One: Cooking Time

You’re cooking pasta for dinner and want to find out how the cooking time affects its texture. What is the independent variable?

Scenario Two: Exercise Routine

You decide to try different exercise routines each week to see which one makes you feel the most energetic. What is the independent variable?

Scenario Three: Plant Fertilizer

You’re growing tomatoes in your garden and decide to use different types of fertilizer to see which one helps them grow the best. What is the independent variable?

Scenario Four: Study Environment

You’re preparing for an important test and try studying in different environments (quiet room, coffee shop, library) to see where you concentrate best. What is the independent variable?

Scenario Five: Sleep Duration

You’re curious to see how the number of hours you sleep each night affects your mood the next day. What is the independent variable?

By practicing identifying independent variables in different scenarios, you’re becoming a true independent variable detective. Keep practicing, stay curious, and you’ll soon be spotting independent variables everywhere you go.

Independent Variable: The cooking time is the independent variable. You are changing the cooking time to observe its effect on the texture of the pasta.

Independent Variable: The type of exercise routine is the independent variable. You are trying out different exercise routines each week to see which one makes you feel the most energetic.

Independent Variable: The type of fertilizer is the independent variable. You are using different types of fertilizer to observe their effects on the growth of the tomatoes.

Independent Variable: The study environment is the independent variable. You are studying in different environments to see where you concentrate best.

Independent Variable: The number of hours you sleep is the independent variable. You are changing your sleep duration to see how it affects your mood the next day.

Whew, what a journey we’ve had exploring the world of independent variables! From understanding their definition and role to diving into a myriad of examples and real-world impacts, we’ve uncovered the treasures hidden in the realm of independent variables.

The beauty of independent variables lies in their ability to unlock new knowledge and insights, guiding us to discoveries that improve our lives and the world around us.

By identifying and studying these variables, we embark on exciting learning adventures, solving mysteries and answering questions about the universe we live in.

Remember, the joy of discovery doesn’t end here. The world is brimming with questions waiting to be answered and mysteries waiting to be solved.

Keep your curiosity alive, continue exploring, and who knows what incredible discoveries lie ahead.

Related posts:

• Confounding Variable in Psychology (Examples + Definition)
• 19+ Experimental Design Examples (Methods + Types)
• Variable Interval Reinforcement Schedule (Examples)
• Variable Ratio Reinforcement Schedule (Examples)
• State Dependent Memory + Learning (Definition and Examples)

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• Knowledge Base
• Methodology
• Independent vs Dependent Variables | Definition & Examples

Independent vs Dependent Variables | Definition & Examples

Published on 4 May 2022 by Pritha Bhandari . Revised on 17 October 2022.

In research, variables are any characteristics that can take on different values, such as height, age, temperature, or test scores.

Researchers often manipulate or measure independent and dependent variables in studies to test cause-and-effect relationships.

• The independent variable is the cause. Its value is independent of other variables in your study.
• The dependent variable is the effect. Its value depends on changes in the independent variable.

Your independent variable is the temperature of the room. You vary the room temperature by making it cooler for half the participants, and warmer for the other half.

Table of contents

What is an independent variable, types of independent variables, what is a dependent variable, identifying independent vs dependent variables, independent and dependent variables in research, visualising independent and dependent variables, frequently asked questions about independent and dependent variables.

An independent variable is the variable you manipulate or vary in an experimental study to explore its effects. It’s called ‘independent’ because it’s not influenced by any other variables in the study.

Independent variables are also called:

• Explanatory variables (they explain an event or outcome)
• Predictor variables (they can be used to predict the value of a dependent variable)
• Right-hand-side variables (they appear on the right-hand side of a regression equation).

These terms are especially used in statistics , where you estimate the extent to which an independent variable change can explain or predict changes in the dependent variable.

Prevent plagiarism, run a free check.

There are two main types of independent variables.

• Experimental independent variables can be directly manipulated by researchers.
• Subject variables cannot be manipulated by researchers, but they can be used to group research subjects categorically.

Experimental variables

In experiments, you manipulate independent variables directly to see how they affect your dependent variable. The independent variable is usually applied at different levels to see how the outcomes differ.

You can apply just two levels in order to find out if an independent variable has an effect at all.

You can also apply multiple levels to find out how the independent variable affects the dependent variable.

You have three independent variable levels, and each group gets a different level of treatment.

You randomly assign your patients to one of the three groups:

• A low-dose experimental group
• A high-dose experimental group
• A placebo group

A true experiment requires you to randomly assign different levels of an independent variable to your participants.

Random assignment helps you control participant characteristics, so that they don’t affect your experimental results. This helps you to have confidence that your dependent variable results come solely from the independent variable manipulation.

Subject variables

Subject variables are characteristics that vary across participants, and they can’t be manipulated by researchers. For example, gender identity, ethnicity, race, income, and education are all important subject variables that social researchers treat as independent variables.

It’s not possible to randomly assign these to participants, since these are characteristics of already existing groups. Instead, you can create a research design where you compare the outcomes of groups of participants with characteristics. This is a quasi-experimental design because there’s no random assignment.

Your independent variable is a subject variable, namely the gender identity of the participants. You have three groups: men, women, and other.

Your dependent variable is the brain activity response to hearing infant cries. You record brain activity with fMRI scans when participants hear infant cries without their awareness.

A dependent variable is the variable that changes as a result of the independent variable manipulation. It’s the outcome you’re interested in measuring, and it ‘depends’ on your independent variable.

In statistics , dependent variables are also called:

• Response variables (they respond to a change in another variable)
• Outcome variables (they represent the outcome you want to measure)
• Left-hand-side variables (they appear on the left-hand side of a regression equation)

The dependent variable is what you record after you’ve manipulated the independent variable. You use this measurement data to check whether and to what extent your independent variable influences the dependent variable by conducting statistical analyses.

Based on your findings, you can estimate the degree to which your independent variable variation drives changes in your dependent variable. You can also predict how much your dependent variable will change as a result of variation in the independent variable.

Distinguishing between independent and dependent variables can be tricky when designing a complex study or reading an academic paper.

A dependent variable from one study can be the independent variable in another study, so it’s important to pay attention to research design.

Here are some tips for identifying each variable type.

Recognising independent variables

Use this list of questions to check whether you’re dealing with an independent variable:

• Is the variable manipulated, controlled, or used as a subject grouping method by the researcher?
• Does this variable come before the other variable in time?
• Is the researcher trying to understand whether or how this variable affects another variable?

Recognising dependent variables

Check whether you’re dealing with a dependent variable:

• Is this variable measured as an outcome of the study?
• Is this variable dependent on another variable in the study?
• Does this variable get measured only after other variables are altered?

Independent and dependent variables are generally used in experimental and quasi-experimental research.

Here are some examples of research questions and corresponding independent and dependent variables.

For experimental data, you analyse your results by generating descriptive statistics and visualising your findings. Then, you select an appropriate statistical test to test your hypothesis .

The type of test is determined by:

• Your variable types
• Level of measurement
• Number of independent variable levels

You’ll often use t tests or ANOVAs to analyse your data and answer your research questions.

In quantitative research , it’s good practice to use charts or graphs to visualise the results of studies. Generally, the independent variable goes on the x -axis (horizontal) and the dependent variable on the y -axis (vertical).

The type of visualisation you use depends on the variable types in your research questions:

• A bar chart is ideal when you have a categorical independent variable.
• A scatterplot or line graph is best when your independent and dependent variables are both quantitative.

To inspect your data, you place your independent variable of treatment level on the x -axis and the dependent variable of blood pressure on the y -axis.

You plot bars for each treatment group before and after the treatment to show the difference in blood pressure.

An independent variable is the variable you manipulate, control, or vary in an experimental study to explore its effects. It’s called ‘independent’ because it’s not influenced by any other variables in the study.

• Right-hand-side variables (they appear on the right-hand side of a regression equation)

A dependent variable is what changes as a result of the independent variable manipulation in experiments . It’s what you’re interested in measuring, and it ‘depends’ on your independent variable.

In statistics, dependent variables are also called:

Determining cause and effect is one of the most important parts of scientific research. It’s essential to know which is the cause – the independent variable – and which is the effect – the dependent variable.

You want to find out how blood sugar levels are affected by drinking diet cola and regular cola, so you conduct an experiment .

• The type of cola – diet or regular – is the independent variable .
• The level of blood sugar that you measure is the dependent variable – it changes depending on the type of cola.

Yes, but including more than one of either type requires multiple research questions .

For example, if you are interested in the effect of a diet on health, you can use multiple measures of health: blood sugar, blood pressure, weight, pulse, and many more. Each of these is its own dependent variable with its own research question.

You could also choose to look at the effect of exercise levels as well as diet, or even the additional effect of the two combined. Each of these is a separate independent variable .

To ensure the internal validity of an experiment , you should only change one independent variable at a time.

No. The value of a dependent variable depends on an independent variable, so a variable cannot be both independent and dependent at the same time. It must be either the cause or the effect, not both.

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Statistics By Jim

Making statistics intuitive

Independent and Dependent Samples in Statistics

By Jim Frost 14 Comments

When comparing groups in your data, you can have either independent or dependent samples. The type of samples in your experimental design impacts sample size requirements, statistical power, the proper analysis, and even your study’s costs. Understanding the implications of each type of sample can help you design a better experiment.

In this post, I’ll define independent and dependent samples, explain their pros and cons, highlight the appropriate analyses for each type, and illustrate how dependent groups can increase your statistical power.

A quick note about terminology. In experiments, you measure an outcome variable for people or objects. I’ll refer to subjects throughout this post to refer to both cases. Additionally, I also use samples and groups synonymously. For example, the term “dependent samples” means the same thing as dependent groups.

Independent Samples vs. Dependent Samples

Hypothesis tests and statistical modeling that compare groups have assumptions about the nature of those groups. Choosing the correct test or model depends on knowing which type of groups your experiment has. Additionally, when designing your study, selecting the best type can help you tailor the design to meet your needs.

Independent samples

In independent samples, subjects in one group do not provide information about subjects in other groups. Each group contains different subjects and there is no meaningful way to pair them. Independent groups are more common in hypothesis testing.

For example, the following experiments use independent samples:

• A medication trial has a control group and a treatment group that contain different subjects.
• A study assesses the strength of a part made from different alloys. Each alloy sample contains different parts.

Studies that use independent samples estimate between-subject effects. These effects are the differences between groups, such as the mean difference. For example, in the medication study, the effect is the mean difference between the treatment and control groups. The focus is on comparing group properties rather than individuals. The sample size for this type of study is the total number of subjects in all groups.

Related post : Independent Samples T Test

Dependent samples

Groups are frequently dependent because they contain the same subjects—that’s the most common example. However, that’s not always the case. Groups with different subjects can be dependent samples if the subjects in one group provide information about the subjects in the other group. For example, statisticians often consider different samples that include pairs of siblings to be dependent because one sibling can provide information about another sibling for some measurements. Other studies use matched pairs. In these studies, the researchers deliberately pair subjects with very similar characteristics. While matched pairs are different people, the statistical analysis treats them as the same person because they are intentionally very similar.

For example, the following experiments use dependent samples:

• A training program assessment takes pretest and posttest scores from the same group of people.
• A paint durability study applies different types of paint to portions of the same wooden boards. All paint types on the same board are considered paired.

Studies that use dependent samples estimate within-subject effects. These effects are the differences between paired subjects, such as the subjects’ mean change. For example, the training program assessment estimates the mean change for subjects from the pretest to the posttest. The emphasis is on the differences between paired subjects. The sample size for this type of study is the number of pairs.

Terms such as paired, repeated measurements, within-subject effects, matched pairs, and pretest/posttest indicate that the groups are dependent.

Related post : Paired T Test

Groups in Datasets

Understanding how researchers record the data can also provide hints about the types of groups. For example, the data look similar in the two worksheets below.

For dependent groups, the focus is on the differences between measurements for each subject. Consequently, if you can meaningfully subtract values in a row, that’s a sure sign of dependency. For example, each row represents one individual in the paired dataset, so assessing the difference between values makes sense.

Conversely, for the independent samples dataset, each group contains a different set of individuals that the researchers chose randomly. Each row in this dataset does not pertain to a single subject. Consequently, it does not make sense to subtract the values between pairs of random people.

Pros and Cons of Independent and Dependent Samples

When thinking about comparing groups, you frequently picture independent groups. For instance, when you imagine comparing a treatment group to a control group, you’re probably assuming these groups contain different subjects. However, by understanding the pros and cons of independent and dependent samples, you can design a study to meet your needs more effectively. The best choice depends on the subject matter and requirements of your experiment. Consider the following while deciding your approach.

Advantages of Independent Samples

When your study uses independent samples, you test each subject once. When you’re working with human subjects, a single test can be advantageous for several reasons. With a single assessment per person, you don’t need to worry about subjects learning how to perform better, getting bored with multiple tests, and how the passage of time affects each person. By testing subjects once, you can rule out various time and order effects that can influence how scores change.

When you are testing physical items, you only need to test each item once. If the testing damages or alters the items, it’s not possible to test them multiple times.

Disadvantages of Independent Samples

Because each group contains different subjects, there can be a wide variety of subject specific factors that influence how they respond to the test. While random assignment to groups can reduce systematic differences between groups, these subject specific factors are not controlled.

Differences between participants in the groups can affect the results. Statisticians refer to these differences as participant variables and they include age, gender, and social background, among many other possibilities.

The additional variability that participant variables create reduces statistical power. You generally need larger sample sizes with independent samples.

Advantages of Dependent Samples

The primary advantage of dependent samples is that you measure the same subjects across different conditions, which allows them to be their own controls. They have the same unique mix of participant variables during all measurements, removing them as sources of variation. Keep this lower variability in mind during my practical demonstration later in this post!

For example, in a pretest/posttest analysis, you will see how each subject reacts to both tests. This method allows the study to focus on the changes within individuals rather than differences between groups of different people.

The net effect is a gain in statistical power. You generally need smaller sample sizes with dependent groups. Additionally, reducing the sample size can decrease a study’s costs, which is particularly helpful when it is difficult or expensive to obtain subjects.

Disadvantages of Dependent Samples

When working with human subjects, you will need to test them multiple times with dependent samples. During repeated testing, subjects can learn more about the tests and figure out how to improve their scores; they might get bored with being tested multiple times; or their test scores might change as a natural result of time passing. In other words, the multiple testing and the passage of time become factors than can influence the measurement, potentially making it challenging to isolate the treatment’s effect.

For example, if the test scores for the training program increase from the pretest to the posttest, the training program might not cause the change. Instead, participants might be learning how to take the test better!

Researchers can mitigate some of these problems. For example, they can include control groups for comparison and change the order of tests for subsets of subjects. However, in general, designs that use dependent groups make it easier for alternatives to explain the changes.

In some cases, using dependent samples is not possible. For example, with destructive testing of material objects, you can only test them once!

As a researcher, weigh the benefits and drawbacks of both types of samples. Some types of research will lend themselves to one approach or the other.

Types of Statistical Analyses for Independent and Dependent Groups

After choosing the type of samples and conducting the experiment, you need to use the correct statistical analysis. The table displays pairs of related analyses for independent and dependent samples.

While analyses for dependent groups typically focus on individual changes, McNemar’s test is an exception. That test compares the overall proportions of two dependent groups.

Regression and ANOVA can model both independent and dependent samples. It’s just a matter of specifying the correct model.

Related posts : Repeated Measures ANOVA and How to do t-tests

Example of Dependent Groups and their Extra Statistical Power

I’m closing with an example that illustrates the extra statistical power that dependent samples can provide. Imagine two studies that, by an amazing coincidence, obtain the same measurements exactly. The only difference is that one has independent groups, while the other has dependent groups.

It should go without saying, but I’ll say it anyway—you will never run a 2-sample t-test and a paired t-test on the same dataset in practice. The two designs are entirely incompatible. However, I’m going to do just that to illustrate the difference in power.

For this experiment, we’re assessing a fictional drug that supposedly increases IQ scores. One experiment uses a control group and a treatment group that have different subjects. The other uses the same set of subjects for a pretest and a posttest. You can download the CSV dataset to try it yourself: IndDepSamples .

First, let’s analyze the dataset as a 2-sample t-test.

Ok, now let’s use the paired t-test.

The data are the same for both analyses and the differences between samples are the same (-11.62). The 2-sample t-test uses a sample size of 30 (two groups with 15 per group), while the paired t-test has only 15 subjects, but the researchers test them twice. Why is the paired t-test with the dependent samples statistically significant while the 2-sample t-test with independent samples is not significant?

Understanding the Different Results

The analyses make different assumptions about the nature of the samples. For the 2-sample t-test, the two groups contain entirely different individuals. While the treatment group has a higher mean IQ score than the control group, we don’t know each subject’s starting score because there was no pretest. Perhaps the treatment group started with higher scores by chance? We don’t know for sure if anyone’s scores increased after taking the drug. This uncertainty reduces the test’s power.

On the other hand, the paired t-test assumes that the pretest and posttest scores are from the same people. From the data, we know all 15 participants saw their scores increase from the pretest to the posttest by an average of 11.63 points. That’s a pretty powerful contrast to the independent samples where we don’t know if any IQ scores increased during the study. While we can be reasonably confident that their scores increased, we’re not sure why. It’s possible that their experience taking the pretest helped them do better on the posttest. Tradeoffs! Maybe next time we’ll include a control group and perform repeated measures ANOVA.

For a more statistical explanation, think back to what I said about dependent samples eliminating participant variables as a source of variability. You can see the reduced variability in the statistical output. The 2-sample t-test uses the pooled standard deviation for both groups, which the output indicates is about 19. However, the paired t-test uses the standard deviation of the differences , and that is much lower at only 6.81. In t-tests, variability is noise that can obscure the signal. Consequently, higher variability reduces statistical power. For more information on this aspect, read my post about how t-tests work .

If you’re planning your next study, consider whether you should use independent or dependent samples. Throughout this post, you learned that each approach has its own benefits and drawbacks. Determine which one works best for your study.

Read more about the related topic of independent and identically distributed (IID) data .

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Reader Interactions

May 26, 2021 at 5:07 am

Hello,Jim, thank you for posting this article. After reading this article, I am thinking that maybe you can help to answer my question. My question is how to determine the correct sample size for dependent sampling. looking forward to your reply. Thanks again~

May 26, 2021 at 3:52 pm

You need to perform a power and sample size analysis . Click the link to learn more. This process helps you determine the correct sample size. In your statistical software, you’ll need to specify an analysis appropriate for dependent samples, such as a paired t-test.

I hope that helps!

February 20, 2021 at 8:16 am

Thanks for this post. I am trying to figure out what my sample numbers should be. I am testing team input/process variables to see which correlate with team cohesion. My questionnaire is to multicultural teams. I originally had in mind 30 like that of good grounded theory. My goal is to see what variables cohesive multicultural teams have. I want to make a meaningful contribution with the research. I am surveying missionary teams as they are commonly multicultural and I have access. I am using these teams from multiple organizations in order to get a good representation in my sample. Please give me a little guidance, which I think will help others who read this. I’ve had several others who are looking to do this type of stats/research, and we are all too statistically novice to know what to do. Thanks, in advance.

October 13, 2020 at 11:52 pm

Here’s some questions;

Statistics are used especially in psychology, sociology and economics. Why? Consider, in psychology, why it is paired with experimental method

October 14, 2020 at 8:57 pm

For your question, I’m going to assume you’re referring to inferential statistics because those methods really extend the usefulness of experiments.

Inferential statistics are a set of analyses that allow you to use sample data to draw conclusions about an entire population. These procedures are very important for scientific studies, including in the areas you mention. Imagine a psychology study that is looking at a treatment for a psychological condition. For this study, the scientists will gather a sample of study participants. The scientists don’t want to know whether the treatment works for only this relatively small group of people in the study. That’s not very helpful for everyone else! Instead, these scientists want to understand how the treatment will work in a larger population. They can use inferential statistics to take the results from their sample and generalize them to an entire population. That makes their study much more useful!

By pairing these statistical procedures with experiments, it allows the researchers to draw conclusions about how effective the treatment is for an entire population, not just the small group of subjects in the experiment.

Thanks for writing with the great question!

October 13, 2020 at 11:51 pm

Statistics are often seen as untrustworthy, and used to prove whatever a person may want to prove. What are some of the common suspicions about the use of statistics?

October 14, 2020 at 8:27 pm

Whenever I read about someone’s statistical analysis, my first concern is about how they collected their data. Did they collect their data from a group of friends who already share their opinion? Or, did they randomly sample people? Data collecting can completely change the results of the analysis.

After data collection, I’d want to understand the specifics about how they analyzed their data. Many analyses can be twisted or misused to give whatever answer the person wants. However, if you collect data properly and use analyses properly, statistics tend to give the correct answers. But, it’s important to understand all the details about how they arrive at their conclusions.

Finally, the best way to protect yourself from someone else misusing statistics is to become knowledgeable in statistics yourself. By understanding statistics, you’ll be able to know what to look for to see through someone else’s statistical trickery!

Thanks for writing and best wishes!

September 7, 2020 at 11:42 am

Thank you Jim. I have benefited from your article

September 2, 2020 at 8:06 pm

Thanks for that Jim. I will give the article a read with that in mind. Tony

September 2, 2020 at 11:49 am

Thank you, Jim. This is an excellent refresher on t-tests and introduces new terminology, i.e., dependent and independent samples. It’s always good to look “under the hood” and see how things work.

September 2, 2020 at 6:12 am

Hi Jim, before I try to wade through your lengthy article, right away I feel this is not the way my study of statistics is going.

My direction, post college statistics, is now data science, machine learning supervised learning alogorithms, as with logistic regression. What little I’ve learned about unsupervised learning is a single project using k-means clustering.

What I know about data sets are pre-processing, data wrangling, modeling and cross validation.

Part of the data wrangling process includes choosing among variables a dependent target variable.

When I saw your title Dependent Samples, I need to understand what is the value here for me.

Let me guess you write for purposes of medical research, not analytics in business.

Thank you for your time.

September 2, 2020 at 2:51 pm

Thanks for your thoughts. Every time I write a blog post, I have no doubt that it will be more helpful for some and less helpful for others. Everyone has their own unique needs. Such is life. I’m sorry this post didn’t help you specifically, but I’m sure it helped others.

I write about all sorts of topics that will be helpful for people learning statistics in a broad range of contexts, including business and machine learning. In this post, some of the content focuses on issues for designing experiments. While that seems to be relevant to scientific fields, many businesses also design experiments. Additionally, I’m sure businesses collect multiple measurements on subjects. This post addresses how that affects the analyses you must use, how to interpret the results, as well as the benefits and risks in terms of explaining the results. This information should be useful in many contexts, such as businesses.

At some point, I plan to write about analyses such as k-means clustering.

Finally, every time I write a post, I include what it’ll be about and the benefits of reading the post right at the beginning. This information allows everyone to decide for themselves if they should read it.

September 2, 2020 at 4:22 am

Thank You for such a great effort in the area of statistics. Well done! Keep it up! Best Wishes!

September 2, 2020 at 1:58 am

Thank you very much Mr. Jim for your effort of sharing your knowledge about the concepts of statistics area.

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• Knowledge Base
• Two-Way ANOVA | Examples & When To Use It

Two-Way ANOVA | Examples & When To Use It

Published on March 20, 2020 by Rebecca Bevans . Revised on June 22, 2023.

ANOVA (Analysis of Variance) is a statistical test used to analyze the difference between the means of more than two groups.

A two-way ANOVA is used to estimate how the mean of a quantitative variable changes according to the levels of two categorical variables. Use a two-way ANOVA when you want to know how two independent variables, in combination, affect a dependent variable.

Table of contents

When to use a two-way anova, how does the anova test work, assumptions of the two-way anova, how to perform a two-way anova, interpreting the results of a two-way anova, how to present the results of a a two-way anova, other interesting articles, frequently asked questions about two-way anova.

You can use a two-way ANOVA when you have collected data on a quantitative dependent variable at multiple levels of two categorical independent variables.

A quantitative variable represents amounts or counts of things. It can be divided to find a group mean.

A categorical variable represents types or categories of things. A level is an individual category within the categorical variable.

You should have enough observations in your data set to be able to find the mean of the quantitative dependent variable at each combination of levels of the independent variables.

Both of your independent variables should be categorical. If one of your independent variables is categorical and one is quantitative, use an ANCOVA instead.

Prevent plagiarism. Run a free check.

ANOVA tests for significance using the F test for statistical significance . The F test is a groupwise comparison test, which means it compares the variance in each group mean to the overall variance in the dependent variable.

If the variance within groups is smaller than the variance between groups, the F test will find a higher F value, and therefore a higher likelihood that the difference observed is real and not due to chance.

A two-way ANOVA with interaction tests three null hypotheses at the same time:

• There is no difference in group means at any level of the first independent variable.
• There is no difference in group means at any level of the second independent variable.
• The effect of one independent variable does not depend on the effect of the other independent variable (a.k.a. no interaction effect).

A two-way ANOVA without interaction (a.k.a. an additive two-way ANOVA) only tests the first two of these hypotheses.

To use a two-way ANOVA your data should meet certain assumptions.Two-way ANOVA makes all of the normal assumptions of a parametric test of difference:

• Homogeneity of variance (a.k.a. homoscedasticity )

The variation around the mean for each group being compared should be similar among all groups. If your data don’t meet this assumption, you may be able to use a non-parametric alternative , like the Kruskal-Wallis test.

• Independence of observations

Your independent variables should not be dependent on one another (i.e. one should not cause the other). This is impossible to test with categorical variables – it can only be ensured by good experimental design .

In addition, your dependent variable should represent unique observations – that is, your observations should not be grouped within locations or individuals.

If your data don’t meet this assumption (i.e. if you set up experimental treatments within blocks), you can include a blocking variable and/or use a repeated-measures ANOVA.

• Normally-distributed dependent variable

The values of the dependent variable should follow a bell curve (they should be normally distributed ). If your data don’t meet this assumption, you can try a data transformation.

The dataset from our imaginary crop yield experiment includes observations of:

• Final crop yield (bushels per acre)
• Type of fertilizer used (fertilizer type 1, 2, or 3)
• Planting density (1=low density, 2=high density)
• Block in the field (1, 2, 3, 4).

The two-way ANOVA will test whether the independent variables (fertilizer type and planting density) have an effect on the dependent variable (average crop yield). But there are some other possible sources of variation in the data that we want to take into account.

We applied our experimental treatment in blocks, so we want to know if planting block makes a difference to average crop yield. We also want to check if there is an interaction effect between two independent variables – for example, it’s possible that planting density affects the plants’ ability to take up fertilizer.

Because we have a few different possible relationships between our variables, we will compare three models:

• A two-way ANOVA without any interaction or blocking variable (a.k.a an additive two-way ANOVA).
• A two-way ANOVA with interaction but with no blocking variable.
• A two-way ANOVA with interaction and with the blocking variable.

Model 1 assumes there is no interaction between the two independent variables. Model 2 assumes that there is an interaction between the two independent variables. Model 3 assumes there is an interaction between the variables, and that the blocking variable is an important source of variation in the data.

By running all three versions of the two-way ANOVA with our data and then comparing the models, we can efficiently test which variables, and in which combinations, are important for describing the data, and see whether the planting block matters for average crop yield.

This is not the only way to do your analysis, but it is a good method for efficiently comparing models based on what you think are reasonable combinations of variables.

Running a two-way ANOVA in R

We will run our analysis in R. To try it yourself, download the sample dataset.

Sample dataset for a two-way ANOVA

After loading the data into the R environment, we will create each of the three models using the aov() command, and then compare them using the aictab() command. For a full walkthrough, see our guide to ANOVA in R .

This first model does not predict any interaction between the independent variables, so we put them together with a ‘+’.

In the second model, to test whether the interaction of fertilizer type and planting density influences the final yield, use a ‘ * ‘ to specify that you also want to know the interaction effect.

Because our crop treatments were randomized within blocks, we add this variable as a blocking factor in the third model. We can then compare our two-way ANOVAs with and without the blocking variable to see whether the planting location matters.

Model comparison

Now we can find out which model is the best fit for our data using AIC ( Akaike information criterion ) model selection.

AIC calculates the best-fit model by finding the model that explains the largest amount of variation in the response variable while using the fewest parameters. We can perform a model comparison in R using the aictab() function.

The output looks like this:

The AIC model with the best fit will be listed first, with the second-best listed next, and so on. This comparison reveals that the two-way ANOVA without any interaction or blocking effects is the best fit for the data.

You can view the summary of the two-way model in R using the summary() command. We will take a look at the results of the first model, which we found was the best fit for our data.

The model summary first lists the independent variables being tested (‘fertilizer’ and ‘density’). Next is the residual variance (‘Residuals’), which is the variation in the dependent variable that isn’t explained by the independent variables.

The following columns provide all of the information needed to interpret the model:

• Df shows the degrees of freedom for each variable (number of levels in the variable minus 1).
• Sum sq is the sum of squares (a.k.a. the variation between the group means created by the levels of the independent variable and the overall mean).
• Mean sq shows the mean sum of squares (the sum of squares divided by the degrees of freedom).
• F value is the test statistic from the F test (the mean square of the variable divided by the mean square of each parameter).
• Pr(>F) is the p value of the F statistic, and shows how likely it is that the F value calculated from the F test would have occurred if the null hypothesis of no difference was true.

From this output we can see that both fertilizer type and planting density explain a significant amount of variation in average crop yield ( p values < 0.001).

Post-hoc testing

ANOVA will tell you which parameters are significant, but not which levels are actually different from one another. To test this we can use a post-hoc test. The Tukey’s Honestly-Significant-Difference (TukeyHSD) test lets us see which groups are different from one another.

This output shows the pairwise differences between the three types of fertilizer ($fertilizer) and between the two levels of planting density ($density), with the average difference (‘diff’), the lower and upper bounds of the 95% confidence interval (‘lwr’ and ‘upr’) and the p value of the difference (‘p-adj’).

From the post-hoc test results, we see that there are significant differences ( p < 0.05) between:

• fertilizer groups 3 and 1,
• fertilizer types 3 and 2,
• the two levels of planting density,

but no difference between fertilizer groups 2 and 1.

Once you have your model output, you can report the results in the results section of your thesis , dissertation or research paper .

When reporting the results you should include the F statistic, degrees of freedom, and p value from your model output.

You can discuss what these findings mean in the discussion section of your paper.

You may also want to make a graph of your results to illustrate your findings.

Your graph should include the groupwise comparisons tested in the ANOVA, with the raw data points, summary statistics (represented here as means and standard error bars), and letters or significance values above the groups to show which groups are significantly different from the others.

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Chi square test of independence
• Statistical power
• Descriptive statistics
• Degrees of freedom
• Pearson correlation
• Null hypothesis

Methodology

• Double-blind study
• Case-control study
• Research ethics
• Data collection
• Hypothesis testing
• Structured interviews

Research bias

• Hawthorne effect
• Unconscious bias
• Recall bias
• Halo effect
• Self-serving bias
• Information bias

The only difference between one-way and two-way ANOVA is the number of independent variables . A one-way ANOVA has one independent variable, while a two-way ANOVA has two.

• One-way ANOVA : Testing the relationship between shoe brand (Nike, Adidas, Saucony, Hoka) and race finish times in a marathon.
• Two-way ANOVA : Testing the relationship between shoe brand (Nike, Adidas, Saucony, Hoka), runner age group (junior, senior, master’s), and race finishing times in a marathon.

All ANOVAs are designed to test for differences among three or more groups. If you are only testing for a difference between two groups, use a t-test instead.

In ANOVA, the null hypothesis is that there is no difference among group means. If any group differs significantly from the overall group mean, then the ANOVA will report a statistically significant result.

Significant differences among group means are calculated using the F statistic, which is the ratio of the mean sum of squares (the variance explained by the independent variable) to the mean square error (the variance left over).

If the F statistic is higher than the critical value (the value of F that corresponds with your alpha value, usually 0.05), then the difference among groups is deemed statistically significant.

A factorial ANOVA is any ANOVA that uses more than one categorical independent variable . A two-way ANOVA is a type of factorial ANOVA.

Some examples of factorial ANOVAs include:

• Testing the combined effects of vaccination (vaccinated or not vaccinated) and health status (healthy or pre-existing condition) on the rate of flu infection in a population.
• Testing the effects of marital status (married, single, divorced, widowed), job status (employed, self-employed, unemployed, retired), and family history (no family history, some family history) on the incidence of depression in a population.
• Testing the effects of feed type (type A, B, or C) and barn crowding (not crowded, somewhat crowded, very crowded) on the final weight of chickens in a commercial farming operation.

Quantitative variables are any variables where the data represent amounts (e.g. height, weight, or age).

Categorical variables are any variables where the data represent groups. This includes rankings (e.g. finishing places in a race), classifications (e.g. brands of cereal), and binary outcomes (e.g. coin flips).

You need to know what type of variables you are working with to choose the right statistical test for your data and interpret your results .

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