BMC Microbiology

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BMC Microbiology is an open access, peer-reviewed journal that considers articles on all microorganisms - bacteria, archaea, algae and fungi, viruses, unicellular parasites and helminths. It considers studies on all aspects of the biology and biochemistry of microorganisms including but not limited to cell biology, genomics, signalling, the interaction of the microbes with the environment and host, mechanistic and functional insights into infection and disease, and biotechnological application in science and industry.

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Citation Impact 2023 Journal Impact Factor: 4.0 5-year Journal Impact Factor: 4.6 Source Normalized Impact per Paper (SNIP): 1.081 SCImago Journal Rank (SJR): 0.999 Speed 2023 Submission to first editorial decision (median days): 15 Submission to acceptance (median days): 135 Usage 2023 Downloads: 2,970,572 Altmetric mentions: 1,619

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ISSN: 1471-2180

Research in Microbiology

journal research in microbiology

Subject Area and Category

  • Molecular Biology
  • Microbiology
  • Medicine (miscellaneous)

Elsevier Masson s.r.l.

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09232508, 17697123

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journal research in microbiology

The set of journals have been ranked according to their SJR and divided into four equal groups, four quartiles. Q1 (green) comprises the quarter of the journals with the highest values, Q2 (yellow) the second highest values, Q3 (orange) the third highest values and Q4 (red) the lowest values.

CategoryYearQuartile
Medicine (miscellaneous)1999Q1
Medicine (miscellaneous)2000Q1
Medicine (miscellaneous)2001Q1
Medicine (miscellaneous)2002Q1
Medicine (miscellaneous)2003Q1
Medicine (miscellaneous)2004Q1
Medicine (miscellaneous)2005Q1
Medicine (miscellaneous)2006Q1
Medicine (miscellaneous)2007Q1
Medicine (miscellaneous)2008Q1
Medicine (miscellaneous)2009Q1
Medicine (miscellaneous)2010Q1
Medicine (miscellaneous)2011Q1
Medicine (miscellaneous)2012Q1
Medicine (miscellaneous)2013Q1
Medicine (miscellaneous)2014Q1
Medicine (miscellaneous)2015Q1
Medicine (miscellaneous)2016Q1
Medicine (miscellaneous)2017Q2
Medicine (miscellaneous)2018Q1
Medicine (miscellaneous)2019Q1
Medicine (miscellaneous)2020Q1
Medicine (miscellaneous)2021Q2
Medicine (miscellaneous)2022Q2
Medicine (miscellaneous)2023Q2
Microbiology1999Q2
Microbiology2000Q2
Microbiology2001Q2
Microbiology2002Q2
Microbiology2003Q2
Microbiology2004Q2
Microbiology2005Q2
Microbiology2006Q1
Microbiology2007Q2
Microbiology2008Q2
Microbiology2009Q2
Microbiology2010Q2
Microbiology2011Q1
Microbiology2012Q1
Microbiology2013Q2
Microbiology2014Q1
Microbiology2015Q2
Microbiology2016Q2
Microbiology2017Q3
Microbiology2018Q2
Microbiology2019Q2
Microbiology2020Q1
Microbiology2021Q2
Microbiology2022Q3
Microbiology2023Q3
Molecular Biology1999Q3
Molecular Biology2000Q3
Molecular Biology2001Q3
Molecular Biology2002Q2
Molecular Biology2003Q2
Molecular Biology2004Q2
Molecular Biology2005Q2
Molecular Biology2006Q2
Molecular Biology2007Q3
Molecular Biology2008Q3
Molecular Biology2009Q2
Molecular Biology2010Q2
Molecular Biology2011Q2
Molecular Biology2012Q2
Molecular Biology2013Q2
Molecular Biology2014Q2
Molecular Biology2015Q3
Molecular Biology2016Q3
Molecular Biology2017Q3
Molecular Biology2018Q2
Molecular Biology2019Q2
Molecular Biology2020Q2
Molecular Biology2021Q3
Molecular Biology2022Q3
Molecular Biology2023Q3

The SJR is a size-independent prestige indicator that ranks journals by their 'average prestige per article'. It is based on the idea that 'all citations are not created equal'. SJR is a measure of scientific influence of journals that accounts for both the number of citations received by a journal and the importance or prestige of the journals where such citations come from It measures the scientific influence of the average article in a journal, it expresses how central to the global scientific discussion an average article of the journal is.

YearSJR
19990.679
20000.727
20010.841
20021.103
20031.116
20041.319
20051.183
20061.418
20071.146
20081.018
20091.169
20101.333
20111.428
20121.373
20131.256
20141.508
20151.089
20161.010
20170.820
20181.045
20191.074
20201.329
20210.860
20220.677
20230.583

Evolution of the number of published documents. All types of documents are considered, including citable and non citable documents.

YearDocuments
199974
2000100
200193
200296
200394
2004112
2005129
2006124
2007102
2008102
2009109
2010113
2011120
201279
2013115
2014119
201589
201683
201787
201877
201950
202044
202149
202240
202394

This indicator counts the number of citations received by documents from a journal and divides them by the total number of documents published in that journal. The chart shows the evolution of the average number of times documents published in a journal in the past two, three and four years have been cited in the current year. The two years line is equivalent to journal impact factor ™ (Thomson Reuters) metric.

Cites per documentYearValue
Cites / Doc. (4 years)19991.249
Cites / Doc. (4 years)20001.524
Cites / Doc. (4 years)20011.532
Cites / Doc. (4 years)20022.090
Cites / Doc. (4 years)20032.267
Cites / Doc. (4 years)20042.606
Cites / Doc. (4 years)20052.853
Cites / Doc. (4 years)20062.826
Cites / Doc. (4 years)20072.863
Cites / Doc. (4 years)20082.636
Cites / Doc. (4 years)20092.794
Cites / Doc. (4 years)20102.929
Cites / Doc. (4 years)20113.063
Cites / Doc. (4 years)20123.392
Cites / Doc. (4 years)20133.413
Cites / Doc. (4 years)20143.403
Cites / Doc. (4 years)20152.718
Cites / Doc. (4 years)20162.647
Cites / Doc. (4 years)20172.419
Cites / Doc. (4 years)20182.836
Cites / Doc. (4 years)20193.286
Cites / Doc. (4 years)20203.929
Cites / Doc. (4 years)20214.616
Cites / Doc. (4 years)20223.677
Cites / Doc. (4 years)20233.355
Cites / Doc. (3 years)19991.249
Cites / Doc. (3 years)20001.572
Cites / Doc. (3 years)20011.616
Cites / Doc. (3 years)20022.232
Cites / Doc. (3 years)20032.304
Cites / Doc. (3 years)20042.777
Cites / Doc. (3 years)20052.550
Cites / Doc. (3 years)20063.015
Cites / Doc. (3 years)20072.704
Cites / Doc. (3 years)20082.465
Cites / Doc. (3 years)20092.649
Cites / Doc. (3 years)20102.693
Cites / Doc. (3 years)20113.105
Cites / Doc. (3 years)20123.389
Cites / Doc. (3 years)20133.420
Cites / Doc. (3 years)20143.318
Cites / Doc. (3 years)20152.358
Cites / Doc. (3 years)20162.502
Cites / Doc. (3 years)20172.275
Cites / Doc. (3 years)20183.131
Cites / Doc. (3 years)20193.150
Cites / Doc. (3 years)20204.411
Cites / Doc. (3 years)20214.164
Cites / Doc. (3 years)20223.238
Cites / Doc. (3 years)20232.677
Cites / Doc. (2 years)19991.135
Cites / Doc. (2 years)20001.662
Cites / Doc. (2 years)20011.586
Cites / Doc. (2 years)20022.228
Cites / Doc. (2 years)20032.423
Cites / Doc. (2 years)20042.447
Cites / Doc. (2 years)20052.602
Cites / Doc. (2 years)20062.776
Cites / Doc. (2 years)20072.419
Cites / Doc. (2 years)20082.252
Cites / Doc. (2 years)20092.181
Cites / Doc. (2 years)20102.578
Cites / Doc. (2 years)20112.968
Cites / Doc. (2 years)20123.210
Cites / Doc. (2 years)20133.171
Cites / Doc. (2 years)20142.871
Cites / Doc. (2 years)20152.051
Cites / Doc. (2 years)20162.418
Cites / Doc. (2 years)20172.535
Cites / Doc. (2 years)20182.906
Cites / Doc. (2 years)20193.530
Cites / Doc. (2 years)20203.795
Cites / Doc. (2 years)20213.766
Cites / Doc. (2 years)20222.462
Cites / Doc. (2 years)20232.618

Evolution of the total number of citations and journal's self-citations received by a journal's published documents during the three previous years. Journal Self-citation is defined as the number of citation from a journal citing article to articles published by the same journal.

CitesYearValue
Self Cites199915
Self Cites200016
Self Cites200116
Self Cites200212
Self Cites200312
Self Cites200414
Self Cites200522
Self Cites200628
Self Cites200716
Self Cites200816
Self Cites200923
Self Cites201018
Self Cites201113
Self Cites201213
Self Cites201315
Self Cites20148
Self Cites20155
Self Cites201622
Self Cites20179
Self Cites20185
Self Cites20196
Self Cites20203
Self Cites20213
Self Cites20223
Self Cites20234
Total Cites1999296
Total Cites2000360
Total Cites2001391
Total Cites2002596
Total Cites2003666
Total Cites2004786
Total Cites2005770
Total Cites20061010
Total Cites2007987
Total Cites2008875
Total Cites2009869
Total Cites2010843
Total Cites20111006
Total Cites20121159
Total Cites20131067
Total Cites20141042
Total Cites2015738
Total Cites2016808
Total Cites2017662
Total Cites2018811
Total Cites2019778
Total Cites2020944
Total Cites2021712
Total Cites2022463
Total Cites2023356

Evolution of the number of total citation per document and external citation per document (i.e. journal self-citations removed) received by a journal's published documents during the three previous years. External citations are calculated by subtracting the number of self-citations from the total number of citations received by the journal’s documents.

CitesYearValue
External Cites per document19991.186
External Cites per document20001.502
External Cites per document20011.550
External Cites per document20022.187
External Cites per document20032.263
External Cites per document20042.728
External Cites per document20052.477
External Cites per document20062.931
External Cites per document20072.660
External Cites per document20082.420
External Cites per document20092.579
External Cites per document20102.636
External Cites per document20113.065
External Cites per document20123.351
External Cites per document20133.372
External Cites per document20143.293
External Cites per document20152.342
External Cites per document20162.433
External Cites per document20172.244
External Cites per document20183.112
External Cites per document20193.126
External Cites per document20204.397
External Cites per document20214.146
External Cites per document20223.217
External Cites per document20232.647
Cites per document19991.249
Cites per document20001.572
Cites per document20011.616
Cites per document20022.232
Cites per document20032.304
Cites per document20042.777
Cites per document20052.550
Cites per document20063.015
Cites per document20072.704
Cites per document20082.465
Cites per document20092.649
Cites per document20102.693
Cites per document20113.105
Cites per document20123.389
Cites per document20133.420
Cites per document20143.318
Cites per document20152.358
Cites per document20162.502
Cites per document20172.275
Cites per document20183.131
Cites per document20193.150
Cites per document20204.411
Cites per document20214.164
Cites per document20223.238
Cites per document20232.677

International Collaboration accounts for the articles that have been produced by researchers from several countries. The chart shows the ratio of a journal's documents signed by researchers from more than one country; that is including more than one country address.

YearInternational Collaboration
199921.62
200019.00
200117.20
200222.92
200326.60
200427.68
200527.13
200621.77
200721.57
200822.55
200922.02
201027.43
201128.33
201226.58
201333.91
201435.29
201526.97
201632.53
201735.63
201828.57
201930.00
202015.91
202122.45
202212.50
202335.11

Not every article in a journal is considered primary research and therefore "citable", this chart shows the ratio of a journal's articles including substantial research (research articles, conference papers and reviews) in three year windows vs. those documents other than research articles, reviews and conference papers.

DocumentsYearValue
Non-citable documents19991
Non-citable documents20001
Non-citable documents20013
Non-citable documents20025
Non-citable documents20036
Non-citable documents20043
Non-citable documents20052
Non-citable documents20061
Non-citable documents20071
Non-citable documents20085
Non-citable documents200924
Non-citable documents201028
Non-citable documents201125
Non-citable documents20128
Non-citable documents201310
Non-citable documents201415
Non-citable documents201519
Non-citable documents201617
Non-citable documents201713
Non-citable documents20188
Non-citable documents20196
Non-citable documents20205
Non-citable documents20215
Non-citable documents20224
Non-citable documents20232
Citable documents1999236
Citable documents2000228
Citable documents2001239
Citable documents2002262
Citable documents2003283
Citable documents2004280
Citable documents2005300
Citable documents2006334
Citable documents2007364
Citable documents2008350
Citable documents2009304
Citable documents2010285
Citable documents2011299
Citable documents2012334
Citable documents2013302
Citable documents2014299
Citable documents2015294
Citable documents2016306
Citable documents2017278
Citable documents2018251
Citable documents2019241
Citable documents2020209
Citable documents2021166
Citable documents2022139
Citable documents2023131

Ratio of a journal's items, grouped in three years windows, that have been cited at least once vs. those not cited during the following year.

DocumentsYearValue
Uncited documents199993
Uncited documents200081
Uncited documents200180
Uncited documents200281
Uncited documents200388
Uncited documents200472
Uncited documents200585
Uncited documents200667
Uncited documents200793
Uncited documents200891
Uncited documents200977
Uncited documents201067
Uncited documents201175
Uncited documents201260
Uncited documents201364
Uncited documents201458
Uncited documents2015100
Uncited documents201696
Uncited documents201784
Uncited documents201852
Uncited documents201945
Uncited documents202030
Uncited documents202134
Uncited documents202230
Uncited documents202325
Cited documents1999144
Cited documents2000148
Cited documents2001162
Cited documents2002186
Cited documents2003201
Cited documents2004211
Cited documents2005217
Cited documents2006268
Cited documents2007272
Cited documents2008264
Cited documents2009251
Cited documents2010246
Cited documents2011249
Cited documents2012282
Cited documents2013248
Cited documents2014256
Cited documents2015213
Cited documents2016227
Cited documents2017207
Cited documents2018207
Cited documents2019202
Cited documents2020184
Cited documents2021137
Cited documents2022113
Cited documents2023108

Evolution of the percentage of female authors.

YearFemale Percent
199942.80
200035.83
200145.48
200242.28
200344.71
200446.30
200547.16
200647.96
200743.01
200838.42
200938.34
201046.94
201144.87
201243.48
201346.61
201442.80
201543.33
201644.81
201742.75
201843.00
201940.24
202050.70
202152.63
202248.83
202350.49

Evolution of the number of documents cited by public policy documents according to Overton database.

DocumentsYearValue
Overton19990
Overton20000
Overton20010
Overton20020
Overton20030
Overton200410
Overton200512
Overton200612
Overton200712
Overton20086
Overton200911
Overton201011
Overton201112
Overton20127
Overton20135
Overton20146
Overton20156
Overton20162
Overton20176
Overton20183
Overton20190
Overton20200
Overton20210
Overton20220
Overton20230

Evoution of the number of documents related to Sustainable Development Goals defined by United Nations. Available from 2018 onwards.

DocumentsYearValue
SDG201810
SDG201911
SDG20208
SDG202110
SDG20225
SDG202320

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Advancements in immunology and microbiology research: a comprehensive exploration of key areas.

journal research in microbiology

1. Introduction

2. unveiling the potential of bacterial proteins as antibody reagents and engineering chimeric proteins, 3. illuminating pathways in vaccine development and clinical studies, 3.1. an hiv experimental vaccine, 3.2. african swine fever virus vaccine update, 3.3. vaccine development faces challenges in inducing strong immune responses, 3.4. new developments in hiv vaccines and challenges, 3.5. tuberculosis vaccines, 3.6. messenger rna (mrna) vaccines, 4. microbiological insights and antimicrobial resistance surveillance, 4.1. extended-spectrum beta-lactamases (esbls): a global public health challenge, 4.2. types of esbl and mechanisms of resistance, 4.3. detection of esbls in medical institutions, 4.4. methicillin-resistant staphylococcus aureus (mrsa) in the caribbean and globally, 4.5. mechanisms of bacterial resistance, 5. evolution of immunological techniques and advancements in blood banking, 6. immunological techniques’ impact on global health and the support of quantitative data, 7. navigating clinical immunology: from bench to bedside management, 7.1. severe combined immunodeficiency disorders, 7.2. transient hypogammaglobulinemia of infancy, 7.3. chronic granulomatous disease (cgd), 7.4. neuropsychiatric systemic lupus erythematosus (npsle), 7.5. viral infections in children with scid, 8. advancements in cancer research: insights and innovations, 9. conclusions, author contributions, institutional review board statement, informed consent statement, data availability statement, acknowledgments, conflicts of interest.

  • Kraef, C.; Tusch, E.; Singh, S.; Østergaard, L.; Fätkenheuer, G.; Castagna, A.; Moreno, S.; Kusejko, K.; Szetela, B.; Kuznetsova, A.; et al. All-cause and AIDS-related mortality among people with HIV across Europe from 2001 to 2020: Impact of antiretroviral therapy, tuberculosis and regional differences in a multicentre cohort study. Lancet Reg. Health Eur. 2024 , 44 , 100989. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Kim, H.K.; Thammavongsa, V.; Schneewind, O.; Missiakas, D. Recurrent Infections and Immune Evasion Strategies of Staphylococcus Aureus. Curr. Opin. Microbiol. 2012 , 15 , 92–99. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Yazdani, R.; Fekrvand, S.; Shahkarami, S.; Azizi, G.; Moazzami, B.; Abolhassani, H.; Aghamohammadi, A. The Hyper IgM Syndromes: Epidemiology, Pathogenesis, Clinical Manifestations, Diagnosis and Management. Clin. Immunol. 2019 , 198 , 19–30. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Drikic, M.; Olsen, S.; De Buck, J. Detecting Total Immunoglobulins in Diverse Animal Species with a Novel Split Enzymatic Assay. BMC Vet. Res. 2019 , 15 , 374. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • de Lima Cavalcanti, T.Y.V.; Pereira, M.R.; de Paula, S.O.; Franca, R.F.d.O. A Review on Chikungunya Virus Epidemiology, Pathogenesis and Current Vaccine Development. Viruses 2022 , 14 , 969. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Milligan, R.; Paul, M.; Richardson, M.; Neuberger, A. Vaccines for Preventing Typhoid Fever. Cochrane Database Syst. Rev. 2018 , 5 , CD001261. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Kumi Smith, M.; Jewell, B.L.; Hallett, T.B.; Cohen, M.S. Treatment of HIV for the Prevention of Transmission in Discordant Couples and at the Population Level. Adv. Exp. Med. Biol. 2018 , 1075 , 125–162. [ Google Scholar ] [ PubMed ]
  • Husna, A.; Rahman, M.M.; Badruzzaman, A.T.M.; Sikder, M.H.; Islam, M.R.; Rahman, M.T.; Alam, J.; Ashour, H.M. Extended-Spectrum β-Lactamases (ESBL): Challenges and Opportunities. Biomedicines 2023 , 11 , 2937. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Medina, E.; Pieper, D.H. Tackling Threats and Future Problems of Multidrug-Resistant Bacteria. Curr. Top. Microbiol. Immunol. 2016 , 398 , 3–33. [ Google Scholar ] [ PubMed ]
  • Ferrer, E.; Lares, M.; Viettri, M.; Medina, M. Comparison between immunological and molecular techniques for the diagnosis of Chagas disease. Enferm. Infecc. Microbiol. Clin. 2013 , 31 , 277–282. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Storch, E.K.; Custer, B.S.; Jacobs, M.R.; Menitove, J.E.; Mintz, P.D. Review of Current Transfusion Therapy and Blood Banking Practices. Blood Rev. 2019 , 38 , 100593. [ Google Scholar ] [ PubMed ]
  • Mansfield, A.S.; Każarnowicz, A.; Karaseva, N.; Sánchez, A.; De Boer, R.; Andric, Z.; Reck, M.; Atagi, S.; Lee, J.-S.; Garassino, M.; et al. Safety and Patient-Reported Outcomes of Atezolizumab, Carboplatin, and Etoposide in Extensive-Stage Small-Cell Lung Cancer (IMpower133): A Randomized Phase I/III Trial. Ann. Oncol. 2020 , 31 , 310–317. [ Google Scholar ] [ PubMed ]
  • Gennery, A.R. Progress in Treating Chronic Granulomatous Disease. Br. J. Haematol. 2021 , 192 , 251–264. [ Google Scholar ] [ PubMed ]
  • Emsen, A.; Uçaryılmaz, H.; Güler, T.; Artaç, H. Regulatory T and B Cells in Transient Hypogammaglobulinemia of Infancy. Turk. J. Pediatr. 2022 , 64 , 228–238. [ Google Scholar ] [ PubMed ]
  • Yu, H.; Nagafuchi, Y.; Fujio, K. Clinical and Immunological Biomarkers for Systemic Lupus Erythematosus. Biomolecules 2021 , 11 , 928. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Chan, S.-W.B.; Zhong, Y.; Lim, S.C.J.; Poh, S.; Teh, K.L.; Soh, J.Y.; Chong, C.Y.; Thoon, K.C.; Seng, M.; Tan, E.S.; et al. Implementation of Universal Newborn Screening for Severe Combined Immunodeficiency in Singapore While Continuing Routine Bacille-Calmette-Guerin Vaccination Given at Birth. Front. Immunol. 2021 , 12 , 794221. [ Google Scholar ]
  • Iraqi, M.; Edri, A.; Greenshpan, Y.; Goldstein, O.; Ofir, N.; Bolel, P.; Abu Ahmad, M.; Zektser, M.; Campbell, K.S.; Rouvio, O.; et al. Blocking the PCNA/NKp44 Checkpoint to Stimulate NK Cell Responses to Multiple Myeloma. Int. J. Mol. Sci. 2022 , 23 , 4717. [ Google Scholar ] [ CrossRef ]
  • Utsunomiya, A. Progress in Allogeneic Hematopoietic Cell Transplantation in Adult T-Cell Leukemia-Lymphoma. Front. Microbiol. 2019 , 10 , 2235. [ Google Scholar ]
  • Hamal, K.R.; Burgess, S.C.; Pevzner, I.Y.; Erf, G.F. Maternal Antibody Transfer from Dams to Their Egg Yolks, Egg Whites, and Chicks in Meat Lines of Chickens. Poult. Sci. 2006 , 85 , 1364–1372. [ Google Scholar ] [ PubMed ]
  • Bussi, C.; Gutierrez, M.G. Mycobacterium Tuberculosis Infection of Host Cells in Space and Time. FEMS Microbiol. Rev. 2019 , 43 , 341–361. [ Google Scholar ] [ PubMed ]
  • Choe, W.; Durgannavar, T.A.; Chung, S.J. Fc-Binding Ligands of Immunoglobulin G: An Overview of High Affinity Proteins and Peptides. Materials 2016 , 9 , 994. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Kronvall, G. A Surface Component in Group A, C, and G Streptococci with Non-Immune Reactivity for Immunoglobulin G. J. Immunol. 1973 , 111 , 1401–1406. [ Google Scholar ] [ CrossRef ]
  • Björck, L.; Protein, L. A Novel Bacterial Cell Wall Protein with Affinity for Ig L Chains. J. Immunol. 1988 , 140 , 1194–1197. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Justiz-Vaillant, A.A.; Akpaka, P.E.; McFarlane-Anderson, N.; Smikle, M.F. Comparison of Techniques of Detecting Immunoglobulin-Binding Protein Reactivity to Immunoglobulin Produced by Different Avian and Mammalian Species. West Indian Med. J. 2013 , 62 , 12–20. [ Google Scholar ] [ PubMed ]
  • De Château, M.; Nilson, B.H.; Erntell, M.; Myhre, E.; Magnusson, C.G.; Akerström, B.; Björck, L. On the Interaction between Protein L and Immunoglobulins of Various Mammalian Species. Scand. J. Immunol. 1993 , 37 , 399–405. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Stöbel, K.; Schönberg, A.; Staak, C. A New Non-Species Dependent ELISA for Detection of Antibodies to Borrelia Burgdorferi S. L. in Zoo Animals. Int. J. Med. Microbiol. 2002 , 291 (Suppl. S33), 88–99. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zhang, S.Y.; Wei, M.X.; Zhou, Z.Y.; Yu, J.Y.; Shi, X.Q. Prevalence of Antibodies to Toxoplasma Gondii in the Sera of Rare Wildlife in the Shanghai Zoological Garden, People’s Republic of China. Parasitol. Int. 2000 , 49 , 171–174. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Genovese, A.; Bouvet, J.P.; Florio, G.; Lamparter-Schummert, B.; Björck, L.; Marone, G. Bacterial Immunoglobulin Superantigen Proteins A and L Activate Human Heart Mast Cells by Interacting with Immunoglobulin E. Infect. Immun. 2000 , 68 , 5517–5524. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Kozlowski, L.M.; Soulika, A.M.; Silverman, G.J.; Lambris, J.D.; Levinson, A.I. Complement Activation by a B Cell Superantigen. J. Immunol. 1996 , 157 , 1200–1206. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Pinto, N.D.S.; Uplekar, S.D.; Moreira, A.R.; Rao, G.; Frey, D.D. Immunoglobulin G Elution in Protein A Chromatography Employing the Method of Chromatofocusing for Reducing the Co-Elution of Impurities. Biotechnol. Bioeng. 2017 , 114 , 154–162. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Svensson, H.G.; Hoogenboom, H.R.; Sjöbring, U. Protein LA, a Novel Hybrid Protein with Unique Single-Chain Fv Antibody- and Fab-Binding Properties. Eur. J. Biochem. 1998 , 258 , 890–896. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Jiang, S.H.; Wang, J.F.; Xu, R.; Liu, Y.J.; Wang, X.N.; Cao, J.; Zhao, P.; Shen, Y.J.; Yang, T.; Yang, H.; et al. Alternate Arrangement of PpL B3 Domain and SpA D Domain Creates Synergistic Double-Site Binding to VH3 and Vkappa Regions of Fab. DNA Cell Biol. 2008 , 27 , 423–431. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Surolia, A.; Pain, D.; Khan, M.I. Protein A: Nature’s Universal Anti-Antibody. Trends Biochem. Sci. 1982 , 7 , 74–76. [ Google Scholar ] [ CrossRef ]
  • Sakyi, M.E.; Kamio, T.; Kohyama, K.; Rahman, M.M.; Shimizu, K.; Okada, A.; Inoshima, Y. Assessing of the Use of Proteins A, G, and Chimeric Protein AG to Detect Marine Mammal Immunoglobulins. PLoS ONE 2023 , 18 , e0291743. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Yang, Z.; Sun, A.; Zhao, X.; Song, M.; Wei, J.; Wang, J.; Zhao, T.; Xie, Y.; Chen, Z.; Tian, Z.; et al. Preparation and Application of a Beta-D-Glucan Microsphere Conjugated Protein A/G. Int. J. Biol. Macromol. 2020 , 151 , 878–884. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Salimi, K.; Usta, D.D.; Koçer, İ.; Çelik, E.; Tuncel, A. Protein A and Protein A/G Coupled Magnetic SiO2 Microspheres for Affinity Purification of Immunoglobulin G. Int. J. Biol. Macromol. 2018 , 111 , 178–185. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Scheffel, J.; Kanje, S.; Borin, J.; Hober, S. Optimization of a Calcium-Dependent Protein A-Derived Domain for Mild Antibody Purification. MAbs 2019 , 11 , 1492–1501. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zhang, H.; Ainsworth, A.J. Investigation of the Poultry Idiotypic Network Using Pasteurella Multocida. Vet. Immunol. Immunopathol. 1994 , 41 , 73–88. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Vaillant, A.J.; Cosme, B.; Smikle, M.; Pérez, O. Feeding Eggs from Hens Immunized with Specific KLH-Conjugated HIV Peptide Candidate Vaccines to Chicks Induces Specific Anti-HIV gp120 and gp41 Antibodies That Neutralize the Original HIV Antigens. Vaccine Res. 2020 , 7 , 92–96. [ Google Scholar ] [ CrossRef ]
  • Zolla-Pazner, S. A Critical Question for HIV Vaccine Development: Which Antibodies to Induce? Science 2014 , 345 , 167–168. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zhu, J.J. African Swine Fever Vaccinology: The Biological Challenges from Immunological Perspectives. Viruses 2022 , 14 , 2021. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Monteagudo, P.L.; Lacasta, A.; López, E.; Bosch, L.; Collado, J.; Pina-Pedrero, S.; Correa-Fiz, F.; Accensi, F.; Navas, M.J.; Vidal, E.; et al. BA71ΔCD2: A New Recombinant Live Attenuated African Swine Fever Virus with Cross-Protective Capabilities. J. Virol. 2017 , 91 , 10–1128. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Bosch-Camós, L.; López, E.; Navas, M.J.; Pina-Pedrero, S.; Accensi, F.; Correa-Fiz, F.; Park, C.; Carrascal, M.; Domínguez, J.; Salas, M.L.; et al. Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach. Vaccines 2021 , 9 , 29. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zhang, Y.; Li, Q.; Luo, L.; Duan, C.; Shen, J.; Wang, Z. Application of Germline Antibody Features to Vaccine Development, Antibody Discovery, Antibody Optimization and Disease Diagnosis. Biotechnol. Adv. 2023 , 65 , 108143. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Mikocziova, I.; Greiff, V.; Sollid, L.M. Immunoglobulin Germline Gene Variation and Its Impact on Human Disease. Genes Immun. 2021 , 22 , 205–217. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Medina-Ramírez, M.; Sanders, R.W.; Klasse, P.J. Targeting B-Cell Germlines and Focusing Affinity Maturation: The next Hurdles in HIV-1-Vaccine Development? Expert Rev. Vaccines 2014 , 13 , 449–452. [ Google Scholar ] [ CrossRef ]
  • Ahmed, Y.; Tian, M.; Gao, Y. Development of an Anti-HIV Vaccine Eliciting Broadly Neutralizing Antibodies. AIDS Res. Ther. 2017 , 14 , 50. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Andrabi, R.; Bhiman, J.N.; Burton, D.R. Strategies for a Multi-Stage Neutralizing Antibody-Based HIV Vaccine. Curr. Opin. Immunol. 2018 , 53 , 143–151. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Hjelmar, K.J.S.; de Armas, L.R.; Goldberg, E.; Pallikkuth, S.; Mathad, J.; Montepiedra, G.; Gupta, A.; Pahwa, S. Impact of in-utero exposure to HIV and latent TB on infant humoral responses. Front. Immunol. 2024 , 15 , 1423435. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Deng, Y.H.; He, H.Y.; Zhang, F.J. Immunogenicity and Protective Efficacy Conferred by a Novel RecombinantMycobacterium bovisBacillus Calmette-Guérin Strain Expressing Interleukin-12p70 of Human Cytokine and Ag85A ofMycobacterium tuberculosisFusion Protein. Scand. J. Immunol. 2013 , 78 , 497–506. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Khoshnood, S.; Heidary, M.; Haeili, M.; Drancourt, M.; Darban-Sarokhalil, D.; Nasiri, M.J.; Lohrasbi, V. Novel Vaccine Candidates against Mycobacterium Tuberculosis. Int. J. Biol. Macromol. 2018 , 120 , 180–188. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zhang, G.; Tang, T.; Chen, Y.; Huang, X.; Liang, T. mRNA Vaccines in Disease Prevention and Treatment. Signal Transduct. Target. Ther. 2023 , 8 , 365. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Guan, S.; Rosenecker, J. Nanotechnologies in Delivery of mRNA Therapeutics Using Nonviral Vector-Based Delivery Systems. Gene Ther. 2017 , 24 , 133–143. [ Google Scholar ] [ CrossRef ]
  • Schmidt, M.; Heimes, A.-S. Immunomodulating Therapies in Breast Cancer-From Prognosis to Clinical Practice. Cancers 2021 , 13 , 4883. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Paterson, D.L.; Bonomo, R.A. Extended-Spectrum Beta-Lactamases: A Clinical Update. Clin. Microbiol. Rev. 2005 , 18 , 657–686. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Bush, K.; Courvalin, P.; Dantas, G.; Davies, J.; Eisenstein, B.; Huovinen, P.; Jacoby, G.A.; Kishony, R.; Kreiswirth, B.N.; Kutter, E.; et al. Tackling Antibiotic Resistance. Nat. Rev. Microbiol. 2011 , 9 , 894–896. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Pitout, J.D.D.; Laupland, K.B. Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae: An Emerging Public-Health Concern. Lancet Infect. Dis. 2008 , 8 , 159–166. [ Google Scholar ] [ CrossRef ]
  • Rodríguez-Baño, J.; Alcalá, J.C.; Cisneros, J.M.; Grill, F.; Oliver, A.; Horcajada, J.P.; Tórtola, T.; Mirelis, B.; Navarro, G.; Cuenca, M.; et al. Community Infections Caused by Extended-Spectrum β-Lactamase–Producing Escherichia Coli. Arch. Intern. Med. 2008 , 168 , 1897–1902. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Nordmann, P.; Carrer, A. Carbapenemases in enterobacteriaceae. Arch. Pediatr. 2010 , 17 (Suppl. S4), S154–S162. [ Google Scholar ] [ CrossRef ]
  • Akpaka, P.E.; Vaillant, A.; Wilson, C.; Jayaratne, P. Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Bacteria in Trinidad and Tobago. Int. J. Microbiol. 2021 , 2021 , 5582755. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Edward, E.A.; El Shehawy, M.R.; Abouelfetouh, A.; Aboulmagd, E. Phenotypic and Molecular Characterization of Extended Spectrum- and Metallo- Beta Lactamase Producing Pseudomonas Aeruginosa Clinical Isolates from Egypt. Infection 2024 . [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Bush, K.; Jacoby, G.A. Updated Functional Classification of Beta-Lactamases. Antimicrob. Agents Chemother. 2010 , 54 , 969–976. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Neyestanaki, D.K.; Mirsalehian, A.; Rezagholizadeh, F.; Jabalameli, F.; Taherikalani, M.; Emaneini, M. Determination of Extended Spectrum Beta-Lactamases, Metallo-Beta-Lactamases and AmpC-Beta-Lactamases among Carbapenem Resistant Pseudomonas Aeruginosa Isolated from Burn Patients. Burns 2014 , 40 , 1556–1561. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Stewart, A.; Harris, P.; Henderson, A.; Paterson, D. Treatment of Infections by OXA-48-Producing Enterobacteriaceae. Antimicrob. Agents Chemother. 2018 , 62 , e01195-18. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Hamprecht, A.; Sommer, J.; Willmann, M.; Brender, C.; Stelzer, Y.; Krause, F.F.; Tsvetkov, T.; Wild, F.; Riedel-Christ, S.; Kutschenreuter, J.; et al. Pathogenicity of Clinical OXA-48 Isolates and Impact of the OXA-48 IncL Plasmid on Virulence and Bacterial Fitness. Front. Microbiol. 2019 , 10 , 2509. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Smith, C.A.; Stewart, N.K.; Toth, M.; Vakulenko, S.B. Structural Insights into the Mechanism of Carbapenemase Activity of the OXA-48 β-Lactamase. Antimicrob. Agents Chemother. 2019 , 63 , 10–1128. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Christian, N.A.; Roye-Green, K.; Smikle, M. Molecular Epidemiology of Multidrug Resistant Extended Spectrum Beta-Lactamase Producing Klebsiella Pneumoniae at a Jamaican Hospital, 2000–2004. BMC Microbiol. 2010 , 10 , 27. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Miranda-Romero, A.L.; Silva-Sanchez, J.; Garza-Ramos, U.; Barrios, H.; Sánchez-Pérez, A.; Reyna-Flores, F. Molecular Characterization of ESBL-Producing Escherichia Coli Isolates from Hospital- and Community-Acquired Infections in NW Mexico. Diagn. Microbiol. Infect. Dis. 2017 , 87 , 49–52. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Gallegos-Miranda, V.; Garza-Ramos, U.; Bolado-Martínez, E.; Navarro-Navarro, M.; Félix-Murray, K.R.; Candia-Plata, M.D.C.; Sanchez-Martinez, G.; Dúran-Bedolla, J.; Silva-Sánchez, J. ESBL-Producing Escherichia Coli and Klebsiella Pneumoniae from Health-Care Institutions in Mexico. J. Chemother. 2021 , 33 , 122–127. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Frediani, A.V.; Svenson, C.S.; Moura, N.O.; Santos, P.R.; Nascente, P.S. ESBL in Positive Hemoculture of a Southern-Brazil Teaching Hospital’s Intensive Care Units. Braz. J. Biol. 2023 , 83 , e269571. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zalegh, I.; Chaoui, L.; Maaloum, F.; Zerouali, K.; Mhand, R.A. Prevalence of Multidrug-Resistant and Extensively Drug-Resistant Phenotypes of Gram-Negative Bacilli Isolated in Clinical Specimens at Centre Hospitalo-Universitaire Ibn Rochd, Morocco. Pan Afr. Med. J. 2023 , 45 , 41. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Monecke, S.; Akpaka, P.E.; Smith, M.R.; Unakal, C.G.; Thoms Rodriguez, C.-A.; Ashraph, K.; Müller, E.; Braun, S.D.; Diezel, C.; Reinicke, M.; et al. Clonal Complexes Distribution of Staphylococcus Aureus Isolates from Clinical Samples from the Caribbean Islands. Antibiotics 2023 , 12 , 1050. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zuma, A.V.P.; Lima, D.F.; Assef, A.P.D.C.; Marques, E.A.; Leão, R.S. Molecular Characterization of Methicillin-Resistant Staphylococcus Aureus Isolated from Blood in Rio de Janeiro Displaying Susceptibility Profiles to Non-β-Lactam Antibiotics. Braz. J. Microbiol. 2017 , 48 , 237–241. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zuo, Q.-F.; Yang, L.-Y.; Feng, Q.; Lu, D.-S.; Dong, Y.-D.; Cai, C.-Z.; Wu, Y.; Guo, Y.; Gu, J.; Zeng, H.; et al. Evaluation of the Protective Immunity of a Novel Subunit Fusion Vaccine in a Murine Model of Systemic MRSA Infection. PLoS ONE 2013 , 8 , e81212. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Bush, K.; Bradford, P.A. β-Lactams and β-Lactamase Inhibitors: An Overview. Cold Spring Harb. Perspect. Med. 2016 , 6 , a025247. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Sandanayaka, V.P.; Prashad, A.S. Resistance to Beta-Lactam Antibiotics: Structure and Mechanism Based Design of Beta-Lactamase Inhibitors. Curr. Med. Chem. 2002 , 9 , 1145–1165. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Hooper, D.C. Mechanisms of Action and Resistance of Older and Newer Fluoroquinolones. Clin. Infect. Dis. 2000 , 31 (Suppl. S2), S24–S28. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Alibert, S.; N’gompaza Diarra, J.; Hernandez, J.; Stutzmann, A.; Fouad, M.; Boyer, G.; Pagès, J.-M. Multidrug Efflux Pumps and Their Role in Antibiotic and Antiseptic Resistance: A Pharmacodynamic Perspective. Expert Opin. Drug Metab. Toxicol. 2017 , 13 , 301–309. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zwe, Y.H.; Chin, S.F.; Kohli, G.S.; Aung, K.T.; Yang, L.; Yuk, H.-G. Whole Genome Sequencing (WGS) Fails to Detect Antimicrobial Resistance (AMR) from Heteroresistant Subpopulation of Salmonella Enterica. Food Microbiol. 2020 , 91 , 103530. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zycka-Krzesinska, J.; Boguslawska, J.; Aleksandrzak-Piekarczyk, T.; Jopek, J.; Bardowski, J.K. Identification and Characterization of Tetracycline Resistance in Lactococcus Lactis Isolated from Polish Raw Milk and Fermented Artisanal Products. Int. J. Food Microbiol. 2015 , 211 , 134–141. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zhou, J.; Song, S.; Xue, S.; Zhu, Y.; Xu, B.; Ma, P.; Lv, Y.; Kang, H. Study of the Epidemiological and Mechanistic Differences Between Carbapenem-Resistant Klebsiella Pneumoniae Infections in Children and Adults. Infect. Drug Resist. 2024 , 17 , 2625–2639. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Koistinen, J. Blood transfusion safety. Duodecim 2004 , 120 , 902–904. [ Google Scholar ] [ PubMed ]
  • Kumrah, R.; Vignesh, P.; Patra, P.; Singh, A.; Anjani, G.; Saini, P.; Sharma, M.; Kaur, A.; Rawat, A. Genetics of Severe Combined Immunodeficiency. Genes Dis. 2020 , 7 , 52–61. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Picker, S.M. Current Methods for the Reduction of Blood-Borne Pathogens: A Comprehensive Literature Review. Blood Transfus. 2013 , 11 , 343–348. [ Google Scholar ] [ PubMed ]
  • Greenwood, B. The Contribution of Vaccination to Global Health: Past, Present and Future. Philos. Trans. R. Soc. Lond. B Biol. Sci. 2014 , 369 , 20130433. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Shimu, A.S.; Wei, H.-X.; Li, Q.; Zheng, X.; Li, B. The New Progress in Cancer Immunotherapy. Clin. Exp. Med. 2023 , 23 , 553–567. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Findeisen, K.E.; Sewell, J.; Ostor, A.J.K. Biological Therapies for Rheumatoid Arthritis: An Overview for the Clinician. Biologics 2021 , 15 , 343–352. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Hamid, O.; Robert, C.; Daud, A.; Hodi, F.S.; Hwu, W.J.; Kefford, R.; Wolchok, J.D.; Hersey, P.; Joseph, R.; Weber, J.S.; et al. Five-Year Survival Outcomes for Patients with Advanced Melanoma Treated with Pembrolizumab in KEYNOTE-001. Ann. Oncol. 2019 , 30 , 582–588. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Frescura, L.; Godfrey-Faussett, P.; Feizzadeh, A.A.; El-Sadr, W.; Syarif, O.; Ghys, P.D.; on and behalf of the 2025 Testing Treatment Target Working Group. Achieving the 95 95 95 Targets for All: A Pathway to Ending AIDS. PLoS ONE 2022 , 17 , e0272405. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Justiz-Vaillant, A.A.; Gopaul, D.; Akpaka, P.E.; Soodeen, S.; Arozarena Fundora, R. Severe Combined Immunodeficiency-Classification, Microbiology Association and Treatment. Microorganisms 2023 , 11 , 1589. [ Google Scholar ] [ CrossRef ]
  • McCusker, C.; Upton, J.; Warrington, R. Primary Immunodeficiency. Allergy Asthma Clin. Immunol. 2018 , 14 , 61. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Basheer, F.; Lee, E.; Liongue, C.; Ward, A.C. Zebrafish Model of Severe Combined Immunodeficiency (SCID) Due to JAK3 Mutation. Biomolecules 2022 , 12 , 1521. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Justiz-Vaillant, A.A.; Hoyte, T.; Davis, N.; Deonarinesingh, C.; De Silva, A.; Dhanpaul, D.; Dookhoo, C.; Doorpat, J.; Dopson, A.; Durgapersad, J.; et al. A Systematic Review of the Clinical Diagnosis of Transient Hypogammaglobulinemia of Infancy. Children 2023 , 10 , 1358. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Moschese, V.; Graziani, S.; Avanzini, M.A.; Carsetti, R.; Marconi, M.; La Rocca, M.; Chini, L.; Pignata, C.; Soresina, A.R.; Consolini, R.; et al. A Prospective Study on Children with Initial Diagnosis of Transient Hypogammaglobulinemia of Infancy: Results from the Italian Primary Immunodeficiency Network. Int. J. Immunopathol. Pharmacol. 2008 , 21 , 343–352. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Justiz-Vaillant, A.A.; Williams-Persad, A.F.-A.; Arozarena-Fundora, R.; Gopaul, D.; Soodeen, S.; Asin-Milan, O.; Thompson, R.; Unakal, C.; Akpaka, P.E. Chronic Granulomatous Disease (CGD): Commonly Associated Pathogens, Diagnosis and Treatment. Microorganisms 2023 , 11 , 2233. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Mortaz, E.; Azempour, E.; Mansouri, D.; Tabarsi, P.; Ghazi, M.; Koenderman, L.; Roos, D.; Adcock, I.M. Common Infections and Target Organs Associated with Chronic Granulomatous Disease in Iran. Int. Arch. Allergy Immunol. 2019 , 179 , 62–73. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Roos, D. Chronic Granulomatous Disease. Br. Med. Bull. 2016 , 118 , 50–63. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Grammatikos, A.; Gennery, A.R. Inflammatory Complications in Chronic Granulomatous Disease. J. Clin. Med. Res. 2024 , 13 , 1092. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Justiz-Vaillant, A.A.; Gopaul, D.; Soodeen, S.; Arozarena-Fundora, R.; Barbosa, O.A.; Unakal, C.; Thompson, R.; Pandit, B.; Umakanthan, S.; Akpaka, P.E. Neuropsychiatric Systemic Lupus Erythematosus: Molecules Involved in Its Imunopathogenesis, Clinical Features, and Treatment. Molecules 2024 , 29 , 747. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Manca, E. Autoantibodies in Neuropsychiatric Systemic Lupus Erythematosus (NPSLE): Can They Be Used as Biomarkers for the Differential Diagnosis of This Disease? Clin. Rev. Allergy Immunol. 2022 , 63 , 194–209. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Schwartz, N.; Stock, A.D.; Putterman, C. Neuropsychiatric Lupus: New Mechanistic Insights and Future Treatment Directions. Nat. Rev. Rheumatol. 2019 , 15 , 137–152. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Stock, A.D.; Wen, J.; Putterman, C. Neuropsychiatric Lupus, the Blood Brain Barrier, and the TWEAK/Fn14 Pathway. Front. Immunol. 2013 , 4 , 484. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Gasparotto, M.; Gatto, M.; Binda, V.; Doria, A.; Moroni, G. Lupus Nephritis: Clinical Presentations and Outcomes in the 21st Century. Rheumatology 2020 , 59 , v39–v51. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Patel, V. The Challenge of Neuropsychiatric Systemic Lupus Erythematosus: From Symptoms to Therapeutic Strategies. Diagnostics 2024 , 14 , 1186. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Wang, M.; Wang, Z.; Zhang, S.; Wu, Y.; Zhang, L.; Zhao, J.; Wang, Q.; Tian, X.; Li, M.; Zeng, X. Progress in the Pathogenesis and Treatment of Neuropsychiatric Systemic Lupus Erythematosus. J. Clin. Med. Res. 2022 , 11 , 4955. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Yu, H.; Zhang, V.W.; Stray-Pedersen, A.; Hanson, I.C.; Forbes, L.R.; de la Morena, M.T.; Chinn, I.K.; Gorman, E.; Mendelsohn, N.J.; Pozos, T.; et al. Rapid Molecular Diagnostics of Severe Primary Immunodeficiency Determined by Using Targeted next-Generation Sequencing. J. Allergy Clin. Immunol. 2016 , 138 , 1142–1151.e2. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Lev, A.; Somech, R.; Somekh, I. Newborn Screening for Severe Combined Immunodeficiency and Inborn Errors of Immunity. Curr. Opin. Pediatr. 2023 , 35 , 692–702. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Wakamatsu, M.; Kojima, D.; Muramatsu, H.; Okuno, Y.; Kataoka, S.; Nakamura, F.; Sakai, Y.; Tsuge, I.; Ito, T.; Ueda, K.; et al. TREC/KREC Newborn Screening Followed by Next-Generation Sequencing for Severe Combined Immunodeficiency in Japan. J. Clin. Immunol. 2022 , 42 , 1696–1707. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Ameratunga, R.; Ahn, Y.; Steele, R.; Woon, S.-T. Transient Hypogammaglobulinaemia of Infancy: Many Patients Recover in Adolescence and Adulthood. Clin. Exp. Immunol. 2019 , 198 , 224–232. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Duse, M.; Iacobini, M.; Leonardi, L.; Smacchia, P.; Antonetti, L.; Giancane, G. Transient Hypogammaglobulinemia of Infancy: Intravenous Immunoglobulin as First Line Therapy. Int. J. Immunopathol. Pharmacol. 2010 , 23 , 349–353. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Bridges, R.A.; Berendes, H.; Good, R.A. A Fatal Granulomatous Disease of Childhood; the Clinical, Pathological, and Laboratory Features of a New Syndrome. AMA J. Dis. Child. 1959 , 97 , 387–408. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Anjani, G.; Vignesh, P.; Joshi, V.; Shandilya, J.K.; Bhattarai, D.; Sharma, J.; Rawat, A. Recent Advances in Chronic Granulomatous Disease. Genes Dis. 2020 , 7 , 84–92. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Mollin, M.; Beaumel, S.; Vigne, B.; Brault, J.; Roux-Buisson, N.; Rendu, J.; Barlogis, V.; Catho, G.; Dumeril, C.; Fouyssac, F.; et al. Clinical, Functional and Genetic Characterization of 16 Patients Suffering from Chronic Granulomatous Disease Variants—Identification of 11 Novel Mutations in CYBB. Clin. Exp. Immunol. 2021 , 203 , 247–266. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Arnold, D.E.; Heimall, J.R. A Review of Chronic Granulomatous Disease. Adv. Ther. 2017 , 34 , 2543–2557. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Yonkof, J.R.; Gupta, A.; Fu, P.; Garabedian, E.; Dalal, J. The United States Immunodeficiency Network Consortium Role of Allogeneic Hematopoietic Stem Cell Transplant for Chronic Granulomatous Disease (CGD): A Report of the United States Immunodeficiency Network. J. Clin. Immunol. 2019 , 39 , 448–458. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Durcan, L.; O’Dwyer, T.; Petri, M. Management Strategies and Future Directions for Systemic Lupus Erythematosus in Adults. Lancet 2019 , 393 , 2332–2343. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Fanouriakis, A.; Tziolos, N.; Bertsias, G.; Boumpas, D.T. Update οn the Diagnosis and Management of Systemic Lupus Erythematosus. Ann. Rheum. Dis. 2021 , 80 , 14–25. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Kivity, S.; Agmon-Levin, N.; Zandman-Goddard, G.; Chapman, J.; Shoenfeld, Y. Neuropsychiatric Lupus: A Mosaic of Clinical Presentations. BMC Med. 2015 , 13 , 43. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zhong, L.; Wang, W.; Ma, M.; Gou, L.; Tang, X.; Song, H. Chronic Active Epstein-Barr Virus Infection as the Initial Symptom in a Janus Kinase 3 Deficiency Child: Case Report and Literature Review. Medicine 2017 , 96 , e7989. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zhang, C.; Liu, T.; Wang, Y.; Chen, W.; Liu, J.; Tao, J.; Zhang, Z.; Zhu, X.; Zhang, Z.; Ming, M.; et al. Metagenomic next-Generation Sequencing of Bronchoalveolar Lavage Fluid from Children with Severe Pneumonia in Pediatric Intensive Care Unit. Front. Cell. Infect. Microbiol. 2023 , 13 , 1082925. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Lauzon, D.; Delage, G.; Brochu, P.; Michaud, J.; Jasmin, G.; Joncas, J.H.; Lapointe, N. Pathogens in Children with Severe Combined Immune Deficiency Disease or AIDS. CMAJ 1986 , 135 , 33–38. [ Google Scholar ] [ PubMed ]
  • Jarvis, W.R.; Middleton, P.J.; Gelfand, E.W. Significance of Viral Infections in Severe Combined Immunodeficiency Disease. Pediatr. Infect. Dis. 1983 , 2 , 187–192. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Jarvis, W.R.; Middleton, P.J.; Gelfand, E.W. Parainfluenza Pneumonia in Severe Combined Immunodeficiency Disease. J. Pediatr. 1979 , 94 , 423–425. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Prince, B.T.; Thielen, B.K.; Williams, K.W.; Kellner, E.S.; Arnold, D.E.; Cosme-Blanco, W.; Redmond, M.T.; Hartog, N.L.; Chong, H.J.; Holland, S.M. Geographic Variability and Pathogen-Specific Considerations in the Diagnosis and Management of Chronic Granulomatous Disease. Pediatr. Health Med. Ther. 2020 , 11 , 257–268. [ Google Scholar ] [ CrossRef ]
  • Chiu, T.L.-H.; Leung, D.; Chan, K.-W.; Yeung, H.M.; Wong, C.-Y.; Mao, H.; He, J.; Vignesh, P.; Liang, W.; Liew, W.K.; et al. Phenomic Analysis of Chronic Granulomatous Disease Reveals More Severe Integumentary Infections in X-Linked Compared With Autosomal Recessive Chronic Granulomatous Disease. Front. Immunol. 2021 , 12 , 803763. [ Google Scholar ] [ CrossRef ]
  • Marciano, B.E.; Spalding, C.; Fitzgerald, A.; Mann, D.; Brown, T.; Osgood, S.; Yockey, L.; Darnell, D.N.; Barnhart, L.; Daub, J.; et al. Common Severe Infections in Chronic Granulomatous Disease. Clin. Infect. Dis. 2015 , 60 , 1176–1183. [ Google Scholar ] [ CrossRef ]
  • Gryboski, J.D.; Pellerano, R.; Young, N.; Edberg, S. Positive Role of Clostridium Difficile Infection in Diarrhea in Infants and Children. Am. J. Gastroenterol. 1991 , 86 , 685–689. [ Google Scholar ] [ PubMed ]
  • Buckley, R.H. Humoral Immunodeficiency. Clin. Immunol. Immunopathol. 1986 , 40 , 13–24. [ Google Scholar ] [ CrossRef ]
  • Zhang, X.; Zhu, L.; Zhang, H.; Chen, S.; Xiao, Y. CAR-T Cell Therapy in Hematological Malignancies: Current Opportunities and Challenges. Front. Immunol. 2022 , 13 , 927153. [ Google Scholar ] [ CrossRef ]
  • Schuster, S.J.; Bishop, M.R.; Tam, C.S.; Waller, E.K.; Borchmann, P.; McGuirk, J.P.; Jäger, U.; Jaglowski, S.; Andreadis, C.; Westin, J.R.; et al. Tisagenlecleucel in Adult Relapsed or Refractory Diffuse Large B-Cell Lymphoma. N. Engl. J. Med. 2019 , 380 , 45–56. [ Google Scholar ] [ CrossRef ]
  • Sang, W.; Shi, M.; Yang, J.; Cao, J.; Xu, L.; Yan, D.; Yao, M.; Liu, H.; Li, W.; Zhang, B.; et al. Phase II Trial of Co-Administration of CD19- and CD20-Targeted Chimeric Antigen Receptor T Cells for Relapsed and Refractory Diffuse Large B Cell Lymphoma. Cancer Med. 2020 , 9 , 5827–5838. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Lindo, L.; Wilkinson, L.H.; Hay, K.A. Befriending the Hostile Tumor Microenvironment in CAR T-Cell Therapy. Front. Immunol. 2020 , 11 , 618387. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Bell, M.; Gottschalk, S. Engineered Cytokine Signaling to Improve CAR T Cell Effector Function. Front. Immunol. 2021 , 12 , 684642. [ Google Scholar ] [ CrossRef ] [ PubMed ]

Click here to enlarge figure

DiseaseDiagnosisManagement/TreatmentReferences
1. Profound deficiencies in T cells, B cells, or both at birth.
2. Due to infections, affected patients usually do not survive beyond infancy.
3. The genetic heterogeneity of SCID frequently delays diagnosis.
4. An NGS-based multigene panel for diagnosing SCID is available.
5. Other problems found in SCID are protein-losing erythroderma, alopecia, hepatosplenomegaly, lymphadenopathies, and severe diarrhoea.
6. TREC/KREC newborn screening.
1. Haematopoietic stem cell transplantation.
2. Antimicrobials.
3. Intravenous immunoglobulins.
4. Supportive therapy, such as nutritional support, aims to provide essential nutrients to maintain or improve a patient’s health.
5. Gene therapy.
[ , , ]
1. THI typically resolves by age four; preterm infants are especially vulnerable to THI.
2. THI, as defined by the WHO and IUIS, is a primary immunodeficiency with reduced immunoglobulin G and A levels.
3. THI diagnosis: the serum IgG levels are two standard deviations below average.
4. THI complications: recurrent infections, prolonged fever, failure to thrive, dermatitis, rhinitis, asthma, and diarrhoea.
5. The isoagglutinin levels and vaccine response are diagnostic tools for THI assessment.
1. IVIG and antibiotic prophylaxis effectively treat THI; immunotherapy reduces allergies.
2. THI can cause infections by Staphylococcus aureus and Streptococcus, treated with antibiotics like amoxicillin or amoxicillin with clavulanate, dosed by age and weight.
[ , ]
1. Recurrent infections by catalase-positive microorganisms like Candida albicans and Staphylococcus aureus are common in CGD.
2. Inflammatory conditions, including bowel inflammatory disease, are associated with CGD.
3. Molecular diagnosis includes next-generation sequencing (NGS), Sanger sequencing, and Genescan analysis.
1. Treatments for CGD include antibacterial prophylaxis with trimethoprim–sulfamethoxazole. Patients with sulfamethoxazole allergy have other options, such as cloxacillin and ciprofloxacin.
2. Antifungal prophylaxis with itraconazole.
3. Interferon gamma immunotherapy.
4. Haematopoietic stem cell transplantation (HCT) is the treatment of choice.
5. Gene therapy is used in a few cases.
[ , , , , ]
1. NPSLE diagnosis depends on clinical signs, symptoms, lab tests, neuroimaging, and histopathology findings, tailored case by case for accuracy.
2. The presence of systemic and anti-CNS antibodies.
3. The presence of headache, psychotic manifestations, mood disorders, convulsions, and other NPSLE manifestations.
4. Testing for anti-dsDNA antibodies.
5. Complement deposition.
1. Antiepileptics, antipsychotics, anxiolytics, mood stabilisers, and antidepressants.
2. Glucocorticoids.
3. Cyclophosphamide, azathioprine, and mycophenolate mofetil.
4. Biologics: rituximab, belimumab, and anifrolumab.
5. Aspirin, heparin, and warfarin.
6. Novel oral anticoagulants: rivaroxaban, apixaban, and edoxaban.
[ , , ]
MicroorganismsSCIDCGDTHI
S. aureus;
Pseudomonas spp.;
Mycobacterium bovis.
Atypical mycobacteria:
Klebsiella pneumoniae;
Pseudomonas aeruginosa;
Burkholderia;
Chryseobacterium.
S. aureus;
Nocardia spp.;
Burkholderia spp.;
Serratia spp.;
Chromobacter spp.;
Salmonella spp.
Streptococcus pneumoniae;
Haemophilus;
influenzae type b;
Pseudomonas aeruginosa;
S. aureus;
Clostridium difficile.
Cytomegalovirus;
Adenovirus;
Enterovirus;
Herpes simplex virus;
Respiratory syncytial virus;
Epstein–Barr virus;
Rotavirus;
Parainfluenza virus.
It is not a primary concern.Respiratory syncytial virus;
Enteroviruses;
Rotavirus.
Pneumocystis jirovecii;
Histoplasma capsulatum;
Cryptococcus neoformans;
Candida albicans;
Aspergillus spp. Acremonium;
Pichia.
Aspergillus spp.;
Candida spp.;
Fusarium dimerum;
Penicillium;
Paecilomyces variotii;
Scedosporium.
Candida spp.
Giardia duodenalis;
Giardia intestinalis;
Cryptosporidium spp.;
Schistosoma species;
Blastocystis hominis;
Fasciola spp.;
Trichostrongylus spp.
Cryptosporidium spp.
It is not a primary concern.Giardia lamblia.
[ , , ][ , , ][ , ]
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Justiz-Vaillant, A.; Gopaul, D.; Soodeen, S.; Unakal, C.; Thompson, R.; Pooransingh, S.; Arozarena-Fundora, R.; Asin-Milan, O.; Akpaka, P.E. Advancements in Immunology and Microbiology Research: A Comprehensive Exploration of Key Areas. Microorganisms 2024 , 12 , 1672. https://doi.org/10.3390/microorganisms12081672

Justiz-Vaillant A, Gopaul D, Soodeen S, Unakal C, Thompson R, Pooransingh S, Arozarena-Fundora R, Asin-Milan O, Akpaka PE. Advancements in Immunology and Microbiology Research: A Comprehensive Exploration of Key Areas. Microorganisms . 2024; 12(8):1672. https://doi.org/10.3390/microorganisms12081672

Justiz-Vaillant, Angel, Darren Gopaul, Sachin Soodeen, Chandrashekhar Unakal, Reinand Thompson, Shalini Pooransingh, Rodolfo Arozarena-Fundora, Odalis Asin-Milan, and Patrick Eberechi Akpaka. 2024. "Advancements in Immunology and Microbiology Research: A Comprehensive Exploration of Key Areas" Microorganisms 12, no. 8: 1672. https://doi.org/10.3390/microorganisms12081672

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Production, purification and characterization of phytase from Pichia kudriavevii FSMP-Y17and its application in layers feed

  • Biotechnology and Industrial Microbiology - Research Paper
  • Published: 20 August 2024

Cite this article

journal research in microbiology

  • Ritu Sharma 1 ,
  • Arpana Mittal 1 ,
  • Varun Gupta 2 &
  • Neeraj K. Aggarwal   ORCID: orcid.org/0000-0003-3515-1207 1  

Introduction

Phytase, recognized for its ability to enhance the nutritional value of phytate-rich foods, has has gained significant prominence. The production of this enzyme has been significantly boosted while preserving economic efficiency by utilizing natural substrates and optimizing essential factors. This study focuses on optimizing phytase production through solid-state fermentation and evaluating its effectiveness in enhancing nutrient utilization in chicken diets.

The objective is to optimize phytase production via solid-state fermentation, characterize purified phytase properties, and assess its impact on nutrient utilization in chicken diets. Through these objectives, we aim to deepen understanding of phytase's role in poultry nutrition and contribute to more efficient feed formulations for improved agricultural outcomes.

Methodology

We utilized solid-state fermentation with Pichia kudriavzevii FSMP-Y17 yeast on orange peel substrate, optimizing variables like temperature, pH, incubation time, and supplementing with glucose and ammonium sulfate. Following fermentation, we purified the phytase enzyme using standard techniques, characterizing its properties, including molecular weight, optimal temperature and pH, substrate affinity, and kinetic parameters.

The optimized conditions yielded a remarkable phytase yield of 7.0 U/gds. Following purification, the enzyme exhibited a molecular weight of 64 kDa and displayed optimal activity at 55 °C and pH 5.5, with kinetic parameters (Km = 3.39 × 10 –3  M and a V max of 7.092 mM/min) indicating efficient substrate affinity.

The addition of purified phytase to chicken diets resulted in significant improvements in nutrient utilization and overall performance, including increased feed intake, improved feed conversion ratio, enhanced bird growth, better phosphorus retention, and improved egg production and quality. By addressing challenges associated with phytate-rich diets, such as reduced nutrient availability and environmental pollution, phytase utilization promotes animal welfare and sustainability in poultry production.

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Abbreviations

Diethylaminoethyl cellulose

Ethylenediaminetetraacetic acid

Feed conversion ratio

β-mercaptoethanol

Polyacrylamide gel electrophoresis

Sodium dodecyl sulphate

Bird Ranjana P, Michael Eskin NA (2021) The emerging role of phosphorus in human health. Adv Food Nutr Res 96:27–88. https://doi.org/10.1016/bs.afnr.2021.02.001

Article   CAS   PubMed   Google Scholar  

Singh G, Kaur G, Williard K, Schoonover J, Nelson KA (2020) Managing phosphorus loss from agroecosystems of the Midwestern United States: A review. Agron J 10(4):561. https://doi.org/10.3390/agronomy10040561

Article   Google Scholar  

Bloot APM, Kalschne DL, Amaral JAS, Baraldi IJ, Canan C (2023) A review of phytic acid sources, obtention, and applications. Food Rev Int 39(1):73–92. https://doi.org/10.1080/87559129.2021.1906697

Article   CAS   Google Scholar  

Rocky-Salimi K, Hashemi M, Safari M, Mousivand M (2016) A novel phytase characterized by thermostability and high pH tolerance from rice phyllosphere isolated Bacillus subtilis BS 46. J Adv Res 7(3):381–390. https://doi.org/10.1016/j.jare.2016.02.003

Article   CAS   PubMed   PubMed Central   Google Scholar  

Sperry J, Davison EK, Neville JC (2023) Phosphorus Sustainability: A Case for Phytic acid as a Biorenewable Platform. Green Chem. https://doi.org/10.1039/D3GC01421E

Alves NM, Guimarães LHS, Piccoli RH, Cardoso PG (2016) Production and partial characterization of an extracellular phytase produced by Muscodor sp. under submerged fermentation. Adv Microbiol 6(1):23–32. https://doi.org/10.4236/aim.2016.61003

Naves LDP, Corrêa AD, Bertechini AG, Gomide EM, dos Santos CD (2012) Effect of ph and temperature on the activity of phytase products used in broiler nutrition. Braz J Poult Sci 14:181–185

Zuo R, Chang J, Yin Q, Chen L, Chen Q, Yang X, Feng H (2010) Phytase gene expression in Lactobacillus and analysis of its biochemical characteristics. Microbiol Res 165(4):329–335. https://doi.org/10.1016/j.micres.2009.06.001

Tian M, Yuan Q (2016) Optimization of phytase production from potato waste using Aspergillus ficuum. 3Biotech 6(2):1–8. https://doi.org/10.1007/s13205-016-0573-9

Wu J, Paudel P, Sun M, Joshi SR, Stout LM, Greiner R, Jaisi DP (2015) Mechanisms and pathways of phytate degradation: Evidence from oxygen isotope ratios of phosphate, HPLC, and phosphorus-31 NMR spectroscopy. Soil Sci Soci of Am J 79(6):1615–1628. https://doi.org/10.2136/sssaj2015.01.0002

Liu X, Han R, Cao Y, Turner BL, Ma LQ (2022) Enhancing Phytate Availability in Soils and Phytate-P Acquisition by Plants: A Review. Environ Sci Technol 56(13):9196–9219. https://doi.org/10.1021/acs.est.2c00099

Moura TF, Reis MP, Horna FA, Nóbrega IPT, Bello A, Donato DC, Sakomura NK (2023) A novel consensus bacterial 6-phytase variant improves the responses of laying hens fed an inorganic phosphorus-free diet with reduced energy and nutrients from 23 to 72 wk of age. Poult Sci 102(10):102949. https://doi.org/10.1016/j.psj.2023.102949

Van Vuuren DP, Bouwman AF, Beusen AH (2010) Phosphorus demand for the 1970–2100 period: a scenario analysis of resource depletion. Glob Environ Change 20(3):428–439. https://doi.org/10.1016/j.gloenvcha.2010.04.004

Kisinyo P, Opala P (2020) Depletion of phosphate rock reserves and world food crisis: reality or hoax?

Adeola O, Cowieson AJ (2011) Board-invited review: opportunities and challenges in using exogenous enzymes to improve nonruminant animal production. J Anim Sci 89(10):3189–3218. https://doi.org/10.2527/jas.2010-3715

Jain J, Kumar A, Singh D, Singh B (2018) Purification and kinetics of a protease-resistant, neutral, and thermostable phytase from Bacillus subtilis subsp. subtilis JJBS250 ameliorating food nutrition. Prep biochem biotechnol 48(8):718–724. https://doi.org/10.1080/10826068.2018.1487848

Ciofalo V, Barton N, Kretz K, Baird J, Cook M, Shanahan D (2003) Safety evaluation of a phytase, expressed in Schizosaccharomyces pombe, intended for use in animal feed. Regul Toxicol Pharmacol 37(2):286–292. https://doi.org/10.1016/S0273-2300(03)00005-9

Yao MZ, Zhang YH, Lu WL, Hu MQ, Wang W, Liang AH (2012) Phytases: crystal structures, protein engineering and potential biotechnological applications. J App Microbiol 112(1):1–14. https://doi.org/10.1111/j.1365-2672.2011.05181.x

Rizwanuddin S, Kumar V, Naik B, Singh P, Mishra S, Rustagi S, Kumar V (2023) Microbial phytase: Their sources, production, and role in the enhancement of nutritional aspects of food and feed additives. J Agri Food Res 12:100559

CAS   Google Scholar  

Kaur P, Kunze G, Satyanarayana T (2007) Yeast phytases: present scenario and future perspectives. Crit Rev Biotechnol 27(2):93–109

Mukherjee V, Radecka D, Aerts G, Verstrepen KJ, Lievens B, Thevelein JM (2017) Phenotypic landscape of non-conventional yeast species for different stress tolerance traits desirable in bioethanol fermentation. Biotechnol biofuels 10:1–19. https://doi.org/10.1186/s13068-017-0899-5

Ogunremi OR, Agrawal R, Sanni A (2020) Production and characterization of volatile compounds and phytase from potentially probiotic yeasts isolated from traditional fermented cereal foods in Nigeria. J Genet Eng Biotechnol 18(1):16. https://doi.org/10.1186/s43141-020-00031-z

Article   PubMed   PubMed Central   Google Scholar  

Greppi A, Krych Ł, Costantini A, Rantsiou K, Hounhouigan DJ, Arneborg N, Jespersen L (2015) Phytase-producing capacity of yeasts isolated from traditional African fermented food products and PHYPk gene expression of Pichia kudriavzevii strains. Int J Food Microbiol 205:81–89. https://doi.org/10.1016/j.ijfoodmicro.2015.04.011

Hellström A, Qvirist L, Svanberg U, Veide Vilg J, Andlid T (2015) Secretion of non-cell-bound phytase by the yeast Pichia kudriavzevii TY13. J Applied Microbiol 118(5):1126–1136. https://doi.org/10.1111/jam.12767

Nakamura Y, Fukuhara H, Sano K (2000) Secreted phytase activities of yeasts. Biosci biotechnol biochem 64(4):841–844. https://doi.org/10.1271/bbb.64.841

Singh PK, Khatta VK, Thakur RS (2003) Effect of phytase supplementation in maize based diet on growth performance and nutrients utilization of broiler chickens. Indian J Anim Sci 73(4):455–458

Dersjant-Li Y, Awati A, Schulze H, Partridge G (2015) Phytase in non-ruminant animal nutrition: a critical review on phytase activities in the gastrointestinal tract and influencing factors. J Sci Food Agric 95(5):878–896

White E, Bold R, Wealleans AL, Dersjant-Li Y, Kwakernaak C (2016) Effect of a Buttiauxella phytase on nutrient digestibility and performance in laying hens fed a diet without supplemental inorganic phosphorus. pp 16–17

Bedford MR, Walk CL, O’Neill HM (2016) Assessing measurements in feed enzyme research: Phytase evaluations in broilers. J App Poult Res 25(2):305–314

Sommerfeld V, Schollenberger M, Kühn I, Rodehutscord M (2018) Interactive effects of phosphorus, calcium, and phytase supplements on products of phytate degradation in the digestive tract of broiler chickens. Poult Sci 97(4):1177–1188

Javadi M, Pascual JJ, Cambra-López M, Macías-Vidal J, Donadeu A, Dupuy J, Cerisuelo A (2021) Effect of dietary mineral content and phytase dose on nutrient utilization, performance, egg traits and bone mineralization in laying hens from 22 to 31 weeks of age. Animals 11(6):1495

Mittal A, Singh G, Goyal V, Yadav A, Aggarwal NK (2012) Isolation and biochemical characterization of acido-thermophilic phytase producer yeast strain for potential application in poultry feed. Adv App Res 4(1):26–34

Google Scholar  

Gulati HK, Chadha BS, Saini HS (2007) Production and characterization of thermostable alkaline phytase from Bacillus laevolacticus isolated from rhizosphere soil. J Ind Microbiol Biotechnol 34(1):91–98

LaemmLi UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685. https://doi.org/10.1038/227680a0

Bae HD, Yanke LJ, Cheng KJ, Selinger LB (1999) A novel staining method for detecting phytase activity. J Microbiol Methods 39(1):17–22. https://doi.org/10.1016/s0167-7012(99)00096-2

Lineweaver H, Burk D (1934) The determination of enzyme dissociation constants. J Amer Chem Soc 56:658–666

Spier MR, Letti LAJ, Woiciechowski AL, Soccol CR (2009) A simplified model for A. niger FS3 growth during phytase formation in solid state fermentation. Braz Arch Bio Technol 52:151–158. https://doi.org/10.1590/S1516-89132009000700020

Liu Z, Qi G, Yoon I (2002) Effect of yeast culture on production parameters and intestinal microflora in laying hens. In: Poultry science association 91st annual meeting abstracts, vol 3

Kumari N, Roy P, Roy S, Parmar PK, Chakraborty S, Das S, Pandey N, Bose A, Bansal AK, Ghosh A (2022) Investigating the Role of the Reduced Solubility of the Pirfenidone-Fumaric Acid Cocrystal in Sustaining the Release Rate from Its Tablet Dosage Form by Conducting Comparative Bioavailability Study in Healthy Human Volunteers. Mol Pharm 19(5):1557–1572. https://doi.org/10.1021/acs.molpharmaceut.2c00052

Bogar B, Szakacs G, Linden JC, Pandey A, Tengerdy RP (2003) Optimization of phytase production by solid substrate fermentation. J Ind Microbiol Biotechnol 30(3):183–189

Berikten D, Kivanc M (2014) Optimization of solid-state fermentation for phytase production by Thermomyces lanuginosus using response surface methodology. Prep Biochem Biotechnol 44(8):834–848

Javed MM, Ahmed W, Zahoor S, Ul-Haq I (2010) Solid state culturing of thermophilic fungi for phytase production. Pak J Bot 42(5):3605–3611

In M, Seo S, Oh N (2008) Fermentative production and application of acid phytase by Saccharomyces cerevisiae CY strain. Afr J Biotechnol 7(17)

Li XY, Liu ZQ, Chi ZM (2008) Production of phytase by a marine yeast Kodamaea ohmeri BG3 in an oats medium: optimization by response surface methodology. Bioresour Technol 99(14):6386–6390. https://doi.org/10.1016/j.biortech.2007.11.065

Nampoothiri KM, Tomes GJ, Roopesh K, Szakacs G, Nagy V, Soccol CR, Pandey A (2004) Thermostable phytase production by Thermoascus aurantiacus in submerged fermentation. Appl biochem biotechnol 118(1–3):205–214. https://doi.org/10.1385/abab:118:1-3:205

Tahir A, Mateen B, Saeed S, Uslu H (2010) Studies on the production of commercially important phytase from Aspergillus niger st-6 isolated from decaying organic soil. Micologia Aplicada Int 22(2):51–57

Kanti A, Idris I, Sudiana IM (2020) Aspergillus niger Str 3 and Neurospora sitophila for phytase production on coconut oil cake supplemented with rice brand in solid-state fermentation. In IOP Conf Ser: Earth Environ Sci 439:012020

Marlida Y, Delfita R, Adnadi P, Ciptaan G (2010) Isolation, characterization and production of phytase from endophytic fungus its application for feed. Pak J Nutr 9(5):471–474

Lee J, Choi Y, Lee PC, Kang S, Bok J, Cho J (2007) Recombinant production of Penicillium oxalicum PJ3 phytase in Pichia pastoris. World J Microbiol Biotechnol 23:443–446

Rodriguez E, Wood ZA, Karplus PA, Lei XG (2000) Site-directed mutagenesis improves catalytic efficiency and thermostability of Escherichia coli pH 2.5 acid phosphatase/phytase expressed in Pichia pastoris. Arch biochem Biophy 382(1):105–112. https://doi.org/10.1006/abbi.2000.2021

Krishna C (2005) Solid-state fermentation systems-an overview. Crit Rev Biotechnol 25(1–2):1–30. https://doi.org/10.1080/07388550590925383

Sato VS, Jorge JA, Guimarães LHS (2016) Characterization of a thermotolerant phytase produced by Rhizopus microsporus var. microsporus biofilm on an inert support using sugarcane bagasse as carbon source. App Biochem Biotechnol 179:610–624

Vohra A, Satyanarayana T (2002) Purification and characterization of a thermostable and acid-stable phytase from Pichia anomala. World J Microbiol Biotechnol 18:687–691

Ebune A, Al-Asheh S, Duvnjak Z (1995) Effects of phosphate, surfactants and glucose on phytase production and hydrolysis of phytic acid in canola meal by Aspergillus ficuum during solid-state fermentation. Bioresour technol 54(3):241–247

Mandviwala TN, Khire JM (2000) Production of high activity thermostable phytase from thermotolerant Aspergillus niger in solid state fermentation. J Ind Microbiol Biotechnol 24:237–243

Battan B, Sharma J, Kuhad RC (2006) High-level xylanase production by alkaliphilic Bacillus pumilus ASH under solid-state fermentation. World J Microbiol Biotechnol 22:1281–1287

Sabu A, Sarita S, Pandey A, Bogar B, Szakacs G, Soccol CR (2002) Solid-state fermentation for production of phytase by Rhizopus oligosporus. App Biochem Biotechnol 102–103(1–6):251–260. https://doi.org/10.1385/abab:102-103:1-6:251

Jafari-Tapeh H, Hamidi-Esfahani Z, Azizi MH (2012) (2012) Culture condition improvement for phytase production in solid state fermentation by Aspergillus ficuum using statistical method. Int Scholarly Res Notices 1:479167

Sasirekha B, Bedashree T, Champa KL (2012) Optimization and partial purification of extracellular phytase from Pseudomonas aeruginosa p6. Europ J Exp Biol 2(1):95–104

Chandrasekharan M, Lakshmanaperumalsamy P, Chandramohan D (1991) Combined effect of environmental factors on spoilage bacteria

Monteiro PS, Guimarães VM, Melo RRD, Rezende STD (2015) Isolation of a thermostable acid phytase from Aspergillus niger UFV-1 with strong proteolysis resistance. Braz J Microbiol 46:251–260

Awad GEA, Helal MMI, Danial EN, Esawy MA (2014) Optimization of phytase production by Penicillium purpurogenum GE1 under solid state fermentation by using Box-Behnken design. Saudi J Biol Sci 21:81–88. https://doi.org/10.1016/j.sjbs.2013.06.004

Siddiqa A, Anwar Z, Rashid, U (2023) Isolation and statistical experimental design for the optimization of phytase production by a newly isolated strain, Aspergillus Terreus (OP028905). Journal of Xi’an Shiyou University, Natural Science Edition 19(6)

Ajith S, Ghosh J, Shet D, ShreeVidhya S, Punith BD, Elangovan AV (2019) Partial purification and characterization of phytase from Aspergillus foetidus MTCC 11682. AMB Express 9(1):1–11

Pavlova K, Gargova S, Hristozova T, Tankova Z (2008) Phytase from Antarctic yeast strain Cryptococcus laurentii AL 27. Folia Microbiol 53:29–34

Kalsi HK, Singh R, Dhaliwal HS, Kumar V (2016) Phytases from Enterobacter and Serratia species with desirable characteristics for food and feed applications. 3Biotech 6(1):1–13

Kim YO, Kim HK, Bae KS, Yu JH, Oh TK (1998) Purification and properties of a thermostable phytase from Bacillus sp. DS11. Enz Microbial Technol 22(1):2–7

Chaphalkar SR, Dey S (1998) Thermostable alkaline metalloprotease from newly isolated alkalophilic Streptomyces diastaticus strain SS1. Indian J Biochem Biophys 35(1):34–40

CAS   PubMed   Google Scholar  

Rodríguez-Fernández DE, Rodríguez-León JA, Carvalho JCD, Thomaz-Soccol V, Parada JL, Soccol CR (2010) Recovery of phytase produced by solid-state fermentation on citrus peel. Braz Arch Bio Technol 53:1487–1496

Ajith S, Shet D, Ghosh J, Elangovan AV (2018) Immobilised phytase production from Aspergillus foetidus MTCC 11682 using an optimized media. Biotechnol J Int 21(3):1–13

Kłosowski G, Mikulski D, Jankowiak O (2018) Extracellular phytase production by the wine yeast S. cerevisiae (Finarome Strain) during submerged fermentation. Molecules 23(4):848

Jalal MA, Scheideler SE (2001) Effect of supplementation of two different sources of phytase on egg production parameters in laying hens and nutrient digestiblity. Poult Sci 80(10):1463–1471

Khalique A, Ahmad F, Muhmud A, Younus M (2010) Effect of locally produced phytase on growth of layer chicks. Pak J Zoo 42(4):445–450

Adekanle MA, Oloke JK, Adekunle OC, Bolaji OS (2023) Effects of phytase producing yeast in poultry feed mills. Annals of West University of Timisoara: Series of Biology 26(1)

Ponnuvel P, Narayankutty K, Jalaludeen A, Anitha P (2015) Effect of phytase supplementation in low energy-protein diet on the production performance of layer chicken. Indian J Vet Sci Biotechnol 10(3):25–27

Simons PC, Versteegh HA, Jongbloed AW, Kemme PA, Slump P, Bos KD, Wolters MG, Beudeker RF, Verschoor GJ (1990) Improvement of phosphorus availability by microbial phytase in broilers and pigs. British J Nutrition 64(2):525–540. https://doi.org/10.1079/bjn19900052

Yoo GY, Wang X, Choi S, Han K, Kang JC, Bai SC (2005) Dietary microbial phytase increased the phosphorus digestibility in juvenile Korean rockfish Sebastes schlegeli fed diets containing soybean meal. Aquaculture 243(1–4):315–322

Bougouin A, Appuhamy JA, Kebreab E, Dijkstra J, Kwakkel RP, France J (2014) Effects of phytase supplementation on phosphorus retention in broilers and layers: a meta-analysis. Poult Sci 93(8):1981–1992. https://doi.org/10.3382/ps.2013-03820

Vohra A, Rastogi SK, Satyanarayana T (2006) Amelioration in growth and phosphorus assimilation of poultry birds using cell-bound phytase of Pichia anomala. World J Microbiol Biotechnol 22:553–558

Fernández SR, Chárraga S, Ávila-Gonzalez E (2019) Evaluation of a new generation phytase on phytate phosphorus release for egg production and tibia strength in hens fed a corn-soybean meal diet. Poult Sci 98(5):2087–2093

Article   PubMed   Google Scholar  

Saleh AA, Elsawee M, Soliman MM, Elkon RY, Alzawqari MH, Shukry M, Eltahan H (2021) Effect of bacterial or fungal phytase supplementation on the performance, egg quality, plasma biochemical parameters, and reproductive morphology of laying hens. Animals 11(2):540

Ren Y, Liu Y, Jiang K, Li L, Jiao N, Zhu Z, Li Y (2023) Effects of low-phosphorus diets supplemented with Phytase on the production performance, phosphorus-calcium metabolism, and bone metabolism of aged Hy-line Brown laying hens. Animals 13(6):1042

Liu N, Liu GH, Li FD, Sands JS, Zhang S, Zheng AJ, Ru YJ (2007) Efficacy of phytases on egg production and nutrient digestibility in layers fed reduced phosphorus diets. Poult Sci 86(11):2337–2342

Daramola OT, Jimoh OA (2015) Egg quality and laying performance of pullets fed different protein sources and Ronozyme-P supplementation. Tropical Animal Prod Investigations 18(1):18–24

Englmaierová M, Skřivan M, Skřivanová E, Čermák L (2017) Limestone particle size and Aspergillus niger phytase in the diet of older hens. Ital J Anim Sci 16(4):608–615

Bello A, Dersjant-Li Y, Korver DR (2020) Effects of dietary calcium and available phosphorus levels and phytase supplementation on performance, bone mineral density, and serum biochemical bone markers in aged white egg-laying hens. Poult Sci 99(11):5792–5801

Eltahan HM, Cho S, Rana MM, Saleh AA, Elkomy AE, Wadaan MA, Eltahan HM (2023) Dietary exogenous phytase improve egg quality, reproductive hormones, and prolongs the lifetime of the aging Hy-Line brown laying hens fed nonphytate phosphorus. Poult Sci 102(9):102895

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Villiger, L. et al. Nat. Rev. Mol. Cell Biol. 25 , 464–487 (2024).

Article   CAS   PubMed   Google Scholar  

Wang, J. Y. & Doudna, J. A. Science 379 , eadd8643 (2023).

Shmakov, S. et al. Mol. Cell 60 , 385–397 (2015).

Article   CAS   PubMed   PubMed Central   Google Scholar  

Altae-Tran, H. et al. Science 382 , eadi1910 (2023).

Hwang, Y., Cornman, A. L., Kellogg, E. H., Ovchinnikov, S. & Girguis, P. R. Nat. Commun. 15 , 2880 (2024).

Boger, R. et al. In 2023 ICML Workshop on Computational Biology https://icml-compbio.github.io/2023/papers/WCBICML2023_paper138.pdf (2023).

Alley, E. C., Khimulya, G., Biswas, S., AlQuraishi, M. & Church, G. M. Nat. Methods 16 , 1315–1322 (2019).

Ruffolo, J. A. et al. Preprint at bioRxiv https://doi.org/10.1101/2024.04.22.590591 (2024).

Nguyen, E. et al. Preprint at bioRxiv https://doi.org/10.1101/2024.02.27.582234 (2024).

Krishna, R. et al. Science 384 , eadl2528 (2024).

Joung, J. et al. Cell 186 , 209–229.e26 (2023).

Replogle, J. M. et al. Cell 185 , 2559–2575.e28 (2022).

Theodoris, C. V. et al. Nature 618 , 616–624 (2023).

Lotfollahi, M., Wolf, F. A. & Theis, F. J. Nat. Methods 16 , 715–721 (2019).

Kedzierska, K. Z., Crawford, L., Amini, A. P. & Lu, A. X. Preprint at bioRxiv https://doi.org/10.1101/2023.10.16.561085 (2023).

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    Journal of Advances in Microbiology Research is a Peer Reviewed International Open Access Journal which will be abstracted in various reputed databases. The Journal provides the platform with the aim of motivating the students and personnel. The journal is indexed in Index Copernicus, Google Scholar, ResearchBib, Geneva Foundation for Medical ...

  23. Research in Microbiology

    Yuanyuan Ma, Xiuqin Wu, Shuying Li, Lie Tang, ... Qianli An. Article 103924. View PDF. Previous vol/issue. Next vol/issue. ISSN: 0923-2508. Read the latest articles of Research in Microbiology at ScienceDirect.com, Elsevier's leading platform of peer-reviewed scholarly literature.

  24. Production, purification and characterization of phytase from Pichia

    Brazilian Journal of Microbiology - Phytase, recognized for its ability to enhance the nutritional value of phytate-rich foods, has has gained significant prominence. ... However, it's crucial to acknowledge the limitations and constraints of this study. Firstly, the research focused primarily on layers, and further investigations are required ...

  25. Surprise finding in study of environmental bacteria could advance

    The research, described August 15 in the journal mBio, suggests that loss of a protein called OpgH in a widely studied bacterium known as Caulobacter crescentus creates a cascade of activity that ...

  26. Current Research in Microbial Sciences

    Current Research in Microbial Sciences is a gold open access (OA) journal, which means articles are permanently and freely available. It is a companion to the highly regarded review journal Current Opinion in Microbiology and is part of the Current Opinion and Research (CO+RE) suite of journals. All CO+RE journals leverage the Current Opinion ...

  27. The FASEB Journal Call for Papers Pain Mechanisms & Management

    The FASEB Journal publishes multidisciplinary basic and translational research covering biology and biomedical sciences at every level of organization. ... encompassing research from the physiological to molecular levels. Research in this issue will focus on: 1) pain physiology and pharmacology, 2) the molecular mechanisms of pain, and 3 ...

  28. Programmable biology through artificial intelligence: from nucleic

    The past century has seen our understanding of biology transformed from phenomenology to programmability. This transformation is the product of several revolutions: mapping the fundamental rules ...