hypothesis vs theory in research

This is the Difference Between a Hypothesis and a Theory

What to Know A hypothesis is an assumption made before any research has been done. It is formed so that it can be tested to see if it might be true. A theory is a principle formed to explain the things already shown in data. Because of the rigors of experiment and control, it is much more likely that a theory will be true than a hypothesis.

As anyone who has worked in a laboratory or out in the field can tell you, science is about process: that of observing, making inferences about those observations, and then performing tests to see if the truth value of those inferences holds up. The scientific method is designed to be a rigorous procedure for acquiring knowledge about the world around us.

In scientific reasoning, a hypothesis is constructed before any applicable research has been done. A theory, on the other hand, is supported by evidence: it's a principle formed as an attempt to explain things that have already been substantiated by data.

Toward that end, science employs a particular vocabulary for describing how ideas are proposed, tested, and supported or disproven. And that's where we see the difference between a hypothesis and a theory .

A hypothesis is an assumption, something proposed for the sake of argument so that it can be tested to see if it might be true.

In the scientific method, the hypothesis is constructed before any applicable research has been done, apart from a basic background review. You ask a question, read up on what has been studied before, and then form a hypothesis.

What is a Hypothesis?

A hypothesis is usually tentative, an assumption or suggestion made strictly for the objective of being tested.

When a character which has been lost in a breed, reappears after a great number of generations, the most probable hypothesis is, not that the offspring suddenly takes after an ancestor some hundred generations distant, but that in each successive generation there has been a tendency to reproduce the character in question, which at last, under unknown favourable conditions, gains an ascendancy. Charles Darwin, On the Origin of Species , 1859 According to one widely reported hypothesis , cell-phone transmissions were disrupting the bees' navigational abilities. (Few experts took the cell-phone conjecture seriously; as one scientist said to me, "If that were the case, Dave Hackenberg's hives would have been dead a long time ago.") Elizabeth Kolbert, The New Yorker , 6 Aug. 2007

What is a Theory?

A theory , in contrast, is a principle that has been formed as an attempt to explain things that have already been substantiated by data. It is used in the names of a number of principles accepted in the scientific community, such as the Big Bang Theory . Because of the rigors of experimentation and control, its likelihood as truth is much higher than that of a hypothesis.

It is evident, on our theory , that coasts merely fringed by reefs cannot have subsided to any perceptible amount; and therefore they must, since the growth of their corals, either have remained stationary or have been upheaved. Now, it is remarkable how generally it can be shown, by the presence of upraised organic remains, that the fringed islands have been elevated: and so far, this is indirect evidence in favour of our theory . Charles Darwin, The Voyage of the Beagle , 1839 An example of a fundamental principle in physics, first proposed by Galileo in 1632 and extended by Einstein in 1905, is the following: All observers traveling at constant velocity relative to one another, should witness identical laws of nature. From this principle, Einstein derived his theory of special relativity. Alan Lightman, Harper's , December 2011

Non-Scientific Use

In non-scientific use, however, hypothesis and theory are often used interchangeably to mean simply an idea, speculation, or hunch (though theory is more common in this regard):

The theory of the teacher with all these immigrant kids was that if you spoke English loudly enough they would eventually understand. E. L. Doctorow, Loon Lake , 1979 Chicago is famous for asking questions for which there can be no boilerplate answers. Example: given the probability that the federal tax code, nondairy creamer, Dennis Rodman and the art of mime all came from outer space, name something else that has extraterrestrial origins and defend your hypothesis . John McCormick, Newsweek , 5 Apr. 1999 In his mind's eye, Miller saw his case suddenly taking form: Richard Bailey had Helen Brach killed because she was threatening to sue him over the horses she had purchased. It was, he realized, only a theory , but it was one he felt certain he could, in time, prove. Full of urgency, a man with a mission now that he had a hypothesis to guide him, he issued new orders to his troops: Find out everything you can about Richard Bailey and his crowd. Howard Blum, Vanity Fair , January 1995

And sometimes one term is used as a genus, or a means for defining the other:

Laplace's popular version of his astronomy, the Système du monde , was famous for introducing what came to be known as the nebular hypothesis , the theory that the solar system was formed by the condensation, through gradual cooling, of the gaseous atmosphere (the nebulae) surrounding the sun. Louis Menand, The Metaphysical Club , 2001 Researchers use this information to support the gateway drug theory — the hypothesis that using one intoxicating substance leads to future use of another. Jordy Byrd, The Pacific Northwest Inlander , 6 May 2015 Fox, the business and economics columnist for Time magazine, tells the story of the professors who enabled those abuses under the banner of the financial theory known as the efficient market hypothesis . Paul Krugman, The New York Times Book Review , 9 Aug. 2009

Incorrect Interpretations of "Theory"

Since this casual use does away with the distinctions upheld by the scientific community, hypothesis and theory are prone to being wrongly interpreted even when they are encountered in scientific contexts—or at least, contexts that allude to scientific study without making the critical distinction that scientists employ when weighing hypotheses and theories.

The most common occurrence is when theory is interpreted—and sometimes even gleefully seized upon—to mean something having less truth value than other scientific principles. (The word law applies to principles so firmly established that they are almost never questioned, such as the law of gravity.)

This mistake is one of projection: since we use theory in general use to mean something lightly speculated, then it's implied that scientists must be talking about the same level of uncertainty when they use theory to refer to their well-tested and reasoned principles.

The distinction has come to the forefront particularly on occasions when the content of science curricula in schools has been challenged—notably, when a school board in Georgia put stickers on textbooks stating that evolution was "a theory, not a fact, regarding the origin of living things." As Kenneth R. Miller, a cell biologist at Brown University, has said , a theory "doesn’t mean a hunch or a guess. A theory is a system of explanations that ties together a whole bunch of facts. It not only explains those facts, but predicts what you ought to find from other observations and experiments.”

While theories are never completely infallible, they form the basis of scientific reasoning because, as Miller said "to the best of our ability, we’ve tested them, and they’ve held up."

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Hypothesis vs. Theory

A hypothesis is either a suggested explanation for an observable phenomenon, or a reasoned prediction of a possible causal correlation among multiple phenomena. In science , a theory is a tested, well-substantiated, unifying explanation for a set of verified, proven factors. A theory is always backed by evidence; a hypothesis is only a suggested possible outcome, and is testable and falsifiable.

Comparison chart

Examples of theory and hypothesis.

Theory: Einstein's theory of relativity is a theory because it has been tested and verified innumerable times, with results consistently verifying Einstein's conclusion. However, simply because Einstein's conclusion has become a theory does not mean testing of this theory has stopped; all science is ongoing. See also the Big Bang theory , germ theory , and climate change .

Hypothesis: One might think that a prisoner who learns a work skill while in prison will be less likely to commit a crime when released. This is a hypothesis, an "educated guess." The scientific method can be used to test this hypothesis, to either prove it is false or prove that it warrants further study. (Note: Simply because a hypothesis is not found to be false does not mean it is true all or even most of the time. If it is consistently true after considerable time and research, it may be on its way to becoming a theory.)

This video further explains the difference between a theory and a hypothesis:

Common Misconception

People often tend to say "theory" when what they're actually talking about is a hypothesis. For instance, "Migraines are caused by drinking coffee after 2 p.m. — well, it's just a theory, not a rule."

This is actually a logically reasoned proposal based on an observation — say 2 instances of drinking coffee after 2 p.m. caused a migraine — but even if this were true, the migraine could have actually been caused by some other factors.

Because this observation is merely a reasoned possibility, it is testable and can be falsified — which makes it a hypothesis, not a theory.

• What is a Scientific Hypothesis? - LiveScience
• Wikipedia:Scientific theory

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Comments: Hypothesis vs Theory

Anonymous comments (2).

October 11, 2013, 1:11pm "In science, a theory is a well-substantiated, unifying explanation for a set of verified, proven hypotheses." But there's no such thing as "proven hypotheses". Hypotheses can be tested/falsified, they can't be "proven". That's just not how science works. Logical deductions based on axioms can be proven, but not scientific hypotheses. On top of that I find it somewhat strange to claim that a theory doesn't have to be testable, if it's built up from hypotheses, which DO have to be testable... — 80.✗.✗.139

May 6, 2014, 11:45pm "Evolution is a theory, not a fact, regarding the origin of living things." this statement is poorly formed because it implies that a thing is a theory until it gets proven and then it is somehow promoted to fact. this is just a misunderstanding of what the words mean, and of how science progresses generally. to say that a theory is inherently dubious because "it isn't a fact" is pretty much a meaningless statement. no expression which qualified as a mere fact could do a very good job of explaining the complicated process by which species have arisen on Earth over the last billion years. in fact, if you claimed that you could come up with such a single fact, now THAT would be dubious! everything we observe in nature supports the theory of evolution, and nothing we observe contradicts it. when you can say this about a theory, it's a pretty fair bet that the theory is correct. — 71.✗.✗.151

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What Is A Research (Scientific) Hypothesis? A plain-language explainer + examples

By:  Derek Jansen (MBA)  | Reviewed By: Dr Eunice Rautenbach | June 2020

If you’re new to the world of research, or it’s your first time writing a dissertation or thesis, you’re probably noticing that the words “research hypothesis” and “scientific hypothesis” are used quite a bit, and you’re wondering what they mean in a research context .

“Hypothesis” is one of those words that people use loosely, thinking they understand what it means. However, it has a very specific meaning within academic research. So, it’s important to understand the exact meaning before you start hypothesizing. 

Research Hypothesis 101

• What is a hypothesis ?
• What is a research hypothesis (scientific hypothesis)?
• Requirements for a research hypothesis
• Definition of a research hypothesis
• The null hypothesis

What is a hypothesis?

Let’s start with the general definition of a hypothesis (not a research hypothesis or scientific hypothesis), according to the Cambridge Dictionary:

Hypothesis: an idea or explanation for something that is based on known facts but has not yet been proved.

In other words, it’s a statement that provides an explanation for why or how something works, based on facts (or some reasonable assumptions), but that has not yet been specifically tested . For example, a hypothesis might look something like this:

Hypothesis: sleep impacts academic performance.

This statement predicts that academic performance will be influenced by the amount and/or quality of sleep a student engages in – sounds reasonable, right? It’s based on reasonable assumptions , underpinned by what we currently know about sleep and health (from the existing literature). So, loosely speaking, we could call it a hypothesis, at least by the dictionary definition.

But that’s not good enough…

Unfortunately, that’s not quite sophisticated enough to describe a research hypothesis (also sometimes called a scientific hypothesis), and it wouldn’t be acceptable in a dissertation, thesis or research paper . In the world of academic research, a statement needs a few more criteria to constitute a true research hypothesis .

What is a research hypothesis?

A research hypothesis (also called a scientific hypothesis) is a statement about the expected outcome of a study (for example, a dissertation or thesis). To constitute a quality hypothesis, the statement needs to have three attributes – specificity , clarity and testability .

Let’s take a look at these more closely.

Need a helping hand?

Hypothesis Essential #1: Specificity & Clarity

A good research hypothesis needs to be extremely clear and articulate about both what’ s being assessed (who or what variables are involved ) and the expected outcome (for example, a difference between groups, a relationship between variables, etc.).

Let’s stick with our sleepy students example and look at how this statement could be more specific and clear.

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.

As you can see, the statement is very specific as it identifies the variables involved (sleep hours and test grades), the parties involved (two groups of students), as well as the predicted relationship type (a positive relationship). There’s no ambiguity or uncertainty about who or what is involved in the statement, and the expected outcome is clear.

Contrast that to the original hypothesis we looked at – “Sleep impacts academic performance” – and you can see the difference. “Sleep” and “academic performance” are both comparatively vague , and there’s no indication of what the expected relationship direction is (more sleep or less sleep). As you can see, specificity and clarity are key.

Hypothesis Essential #2: Testability (Provability)

A statement must be testable to qualify as a research hypothesis. In other words, there needs to be a way to prove (or disprove) the statement. If it’s not testable, it’s not a hypothesis – simple as that.

For example, consider the hypothesis we mentioned earlier:

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.  

We could test this statement by undertaking a quantitative study involving two groups of students, one that gets 8 or more hours of sleep per night for a fixed period, and one that gets less. We could then compare the standardised test results for both groups to see if there’s a statistically significant difference. 

Again, if you compare this to the original hypothesis we looked at – “Sleep impacts academic performance” – you can see that it would be quite difficult to test that statement, primarily because it isn’t specific enough. How much sleep? By who? What type of academic performance?

So, remember the mantra – if you can’t test it, it’s not a hypothesis 🙂

Defining A Research Hypothesis

You’re still with us? Great! Let’s recap and pin down a clear definition of a hypothesis.

A research hypothesis (or scientific hypothesis) is a statement about an expected relationship between variables, or explanation of an occurrence, that is clear, specific and testable.

So, when you write up hypotheses for your dissertation or thesis, make sure that they meet all these criteria. If you do, you’ll not only have rock-solid hypotheses but you’ll also ensure a clear focus for your entire research project.

What about the null hypothesis?

You may have also heard the terms null hypothesis , alternative hypothesis, or H-zero thrown around. At a simple level, the null hypothesis is the counter-proposal to the original hypothesis.

For example, if the hypothesis predicts that there is a relationship between two variables (for example, sleep and academic performance), the null hypothesis would predict that there is no relationship between those variables.

At a more technical level, the null hypothesis proposes that no statistical significance exists in a set of given observations and that any differences are due to chance alone.

And there you have it – hypotheses in a nutshell. 

If you have any questions, be sure to leave a comment below and we’ll do our best to help you. If you need hands-on help developing and testing your hypotheses, consider our private coaching service , where we hold your hand through the research journey.

Psst… there’s more (for free)

This post is part of our dissertation mini-course, which covers everything you need to get started with your dissertation, thesis or research project. 

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16 Comments

Very useful information. I benefit more from getting more information in this regard.

Very great insight,educative and informative. Please give meet deep critics on many research data of public international Law like human rights, environment, natural resources, law of the sea etc

In a book I read a distinction is made between null, research, and alternative hypothesis. As far as I understand, alternative and research hypotheses are the same. Can you please elaborate? Best Afshin

This is a self explanatory, easy going site. I will recommend this to my friends and colleagues.

Very good definition. How can I cite your definition in my thesis? Thank you. Is nul hypothesis compulsory in a research?

It’s a counter-proposal to be proven as a rejection

Please what is the difference between alternate hypothesis and research hypothesis?

It is a very good explanation. However, it limits hypotheses to statistically tasteable ideas. What about for qualitative researches or other researches that involve quantitative data that don’t need statistical tests?

In qualitative research, one typically uses propositions, not hypotheses.

could you please elaborate it more

I’ve benefited greatly from these notes, thank you.

This is very helpful

well articulated ideas are presented here, thank you for being reliable sources of information

Excellent. Thanks for being clear and sound about the research methodology and hypothesis (quantitative research)

I have only a simple question regarding the null hypothesis. – Is the null hypothesis (Ho) known as the reversible hypothesis of the alternative hypothesis (H1? – How to test it in academic research?

this is very important note help me much more

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The Craft of Writing a Strong Hypothesis

Table of Contents

Writing a hypothesis is one of the essential elements of a scientific research paper. It needs to be to the point, clearly communicating what your research is trying to accomplish. A blurry, drawn-out, or complexly-structured hypothesis can confuse your readers. Or worse, the editor and peer reviewers.

A captivating hypothesis is not too intricate. This blog will take you through the process so that, by the end of it, you have a better idea of how to convey your research paper's intent in just one sentence.

What is a Hypothesis?

The first step in your scientific endeavor, a hypothesis, is a strong, concise statement that forms the basis of your research. It is not the same as a thesis statement , which is a brief summary of your research paper .

The sole purpose of a hypothesis is to predict your paper's findings, data, and conclusion. It comes from a place of curiosity and intuition . When you write a hypothesis, you're essentially making an educated guess based on scientific prejudices and evidence, which is further proven or disproven through the scientific method.

The reason for undertaking research is to observe a specific phenomenon. A hypothesis, therefore, lays out what the said phenomenon is. And it does so through two variables, an independent and dependent variable.

The independent variable is the cause behind the observation, while the dependent variable is the effect of the cause. A good example of this is “mixing red and blue forms purple.” In this hypothesis, mixing red and blue is the independent variable as you're combining the two colors at your own will. The formation of purple is the dependent variable as, in this case, it is conditional to the independent variable.

Different Types of Hypotheses‌

Types of hypotheses

Some would stand by the notion that there are only two types of hypotheses: a Null hypothesis and an Alternative hypothesis. While that may have some truth to it, it would be better to fully distinguish the most common forms as these terms come up so often, which might leave you out of context.

Apart from Null and Alternative, there are Complex, Simple, Directional, Non-Directional, Statistical, and Associative and casual hypotheses. They don't necessarily have to be exclusive, as one hypothesis can tick many boxes, but knowing the distinctions between them will make it easier for you to construct your own.

1. Null hypothesis

A null hypothesis proposes no relationship between two variables. Denoted by H 0 , it is a negative statement like “Attending physiotherapy sessions does not affect athletes' on-field performance.” Here, the author claims physiotherapy sessions have no effect on on-field performances. Even if there is, it's only a coincidence.

2. Alternative hypothesis

Considered to be the opposite of a null hypothesis, an alternative hypothesis is donated as H1 or Ha. It explicitly states that the dependent variable affects the independent variable. A good  alternative hypothesis example is “Attending physiotherapy sessions improves athletes' on-field performance.” or “Water evaporates at 100 °C. ” The alternative hypothesis further branches into directional and non-directional.

• Directional hypothesis: A hypothesis that states the result would be either positive or negative is called directional hypothesis. It accompanies H1 with either the ‘<' or ‘>' sign.
• Non-directional hypothesis: A non-directional hypothesis only claims an effect on the dependent variable. It does not clarify whether the result would be positive or negative. The sign for a non-directional hypothesis is ‘≠.'

3. Simple hypothesis

A simple hypothesis is a statement made to reflect the relation between exactly two variables. One independent and one dependent. Consider the example, “Smoking is a prominent cause of lung cancer." The dependent variable, lung cancer, is dependent on the independent variable, smoking.

4. Complex hypothesis

In contrast to a simple hypothesis, a complex hypothesis implies the relationship between multiple independent and dependent variables. For instance, “Individuals who eat more fruits tend to have higher immunity, lesser cholesterol, and high metabolism.” The independent variable is eating more fruits, while the dependent variables are higher immunity, lesser cholesterol, and high metabolism.

5. Associative and casual hypothesis

Associative and casual hypotheses don't exhibit how many variables there will be. They define the relationship between the variables. In an associative hypothesis, changing any one variable, dependent or independent, affects others. In a casual hypothesis, the independent variable directly affects the dependent.

6. Empirical hypothesis

Also referred to as the working hypothesis, an empirical hypothesis claims a theory's validation via experiments and observation. This way, the statement appears justifiable and different from a wild guess.

Say, the hypothesis is “Women who take iron tablets face a lesser risk of anemia than those who take vitamin B12.” This is an example of an empirical hypothesis where the researcher  the statement after assessing a group of women who take iron tablets and charting the findings.

7. Statistical hypothesis

The point of a statistical hypothesis is to test an already existing hypothesis by studying a population sample. Hypothesis like “44% of the Indian population belong in the age group of 22-27.” leverage evidence to prove or disprove a particular statement.

Characteristics of a Good Hypothesis

Writing a hypothesis is essential as it can make or break your research for you. That includes your chances of getting published in a journal. So when you're designing one, keep an eye out for these pointers:

• A research hypothesis has to be simple yet clear to look justifiable enough.
• It has to be testable — your research would be rendered pointless if too far-fetched into reality or limited by technology.
• It has to be precise about the results —what you are trying to do and achieve through it should come out in your hypothesis.
• A research hypothesis should be self-explanatory, leaving no doubt in the reader's mind.
• If you are developing a relational hypothesis, you need to include the variables and establish an appropriate relationship among them.
• A hypothesis must keep and reflect the scope for further investigations and experiments.

Separating a Hypothesis from a Prediction

Outside of academia, hypothesis and prediction are often used interchangeably. In research writing, this is not only confusing but also incorrect. And although a hypothesis and prediction are guesses at their core, there are many differences between them.

A hypothesis is an educated guess or even a testable prediction validated through research. It aims to analyze the gathered evidence and facts to define a relationship between variables and put forth a logical explanation behind the nature of events.

Predictions are assumptions or expected outcomes made without any backing evidence. They are more fictionally inclined regardless of where they originate from.

For this reason, a hypothesis holds much more weight than a prediction. It sticks to the scientific method rather than pure guesswork. "Planets revolve around the Sun." is an example of a hypothesis as it is previous knowledge and observed trends. Additionally, we can test it through the scientific method.

Whereas "COVID-19 will be eradicated by 2030." is a prediction. Even though it results from past trends, we can't prove or disprove it. So, the only way this gets validated is to wait and watch if COVID-19 cases end by 2030.

Finally, How to Write a Hypothesis

Quick tips on writing a hypothesis

1.  Be clear about your research question

A hypothesis should instantly address the research question or the problem statement. To do so, you need to ask a question. Understand the constraints of your undertaken research topic and then formulate a simple and topic-centric problem. Only after that can you develop a hypothesis and further test for evidence.

2. Carry out a recce

Once you have your research's foundation laid out, it would be best to conduct preliminary research. Go through previous theories, academic papers, data, and experiments before you start curating your research hypothesis. It will give you an idea of your hypothesis's viability or originality.

Making use of references from relevant research papers helps draft a good research hypothesis. SciSpace Discover offers a repository of over 270 million research papers to browse through and gain a deeper understanding of related studies on a particular topic. Additionally, you can use SciSpace Copilot , your AI research assistant, for reading any lengthy research paper and getting a more summarized context of it. A hypothesis can be formed after evaluating many such summarized research papers. Copilot also offers explanations for theories and equations, explains paper in simplified version, allows you to highlight any text in the paper or clip math equations and tables and provides a deeper, clear understanding of what is being said. This can improve the hypothesis by helping you identify potential research gaps.

3. Create a 3-dimensional hypothesis

Variables are an essential part of any reasonable hypothesis. So, identify your independent and dependent variable(s) and form a correlation between them. The ideal way to do this is to write the hypothetical assumption in the ‘if-then' form. If you use this form, make sure that you state the predefined relationship between the variables.

In another way, you can choose to present your hypothesis as a comparison between two variables. Here, you must specify the difference you expect to observe in the results.

4. Write the first draft

Now that everything is in place, it's time to write your hypothesis. For starters, create the first draft. In this version, write what you expect to find from your research.

Clearly separate your independent and dependent variables and the link between them. Don't fixate on syntax at this stage. The goal is to ensure your hypothesis addresses the issue.

5. Proof your hypothesis

After preparing the first draft of your hypothesis, you need to inspect it thoroughly. It should tick all the boxes, like being concise, straightforward, relevant, and accurate. Your final hypothesis has to be well-structured as well.

Research projects are an exciting and crucial part of being a scholar. And once you have your research question, you need a great hypothesis to begin conducting research. Thus, knowing how to write a hypothesis is very important.

Now that you have a firmer grasp on what a good hypothesis constitutes, the different kinds there are, and what process to follow, you will find it much easier to write your hypothesis, which ultimately helps your research.

Now it's easier than ever to streamline your research workflow with SciSpace Discover . Its integrated, comprehensive end-to-end platform for research allows scholars to easily discover, write and publish their research and fosters collaboration.

It includes everything you need, including a repository of over 270 million research papers across disciplines, SEO-optimized summaries and public profiles to show your expertise and experience.

If you found these tips on writing a research hypothesis useful, head over to our blog on Statistical Hypothesis Testing to learn about the top researchers, papers, and institutions in this domain.

Frequently Asked Questions (FAQs)

1. what is the definition of hypothesis.

According to the Oxford dictionary, a hypothesis is defined as “An idea or explanation of something that is based on a few known facts, but that has not yet been proved to be true or correct”.

2. What is an example of hypothesis?

The hypothesis is a statement that proposes a relationship between two or more variables. An example: "If we increase the number of new users who join our platform by 25%, then we will see an increase in revenue."

3. What is an example of null hypothesis?

A null hypothesis is a statement that there is no relationship between two variables. The null hypothesis is written as H0. The null hypothesis states that there is no effect. For example, if you're studying whether or not a particular type of exercise increases strength, your null hypothesis will be "there is no difference in strength between people who exercise and people who don't."

4. What are the types of research?

• Fundamental research

• Applied research

• Qualitative research

• Quantitative research

• Mixed research

• Exploratory research

• Longitudinal research

• Cross-sectional research

• Field research

• Laboratory research

• Fixed research

• Flexible research

• Action research

• Policy research

• Classification research

• Comparative research

• Causal research

• Inductive research

• Deductive research

5. How to write a hypothesis?

• Your hypothesis should be able to predict the relationship and outcome.

• Avoid wordiness by keeping it simple and brief.

• Your hypothesis should contain observable and testable outcomes.

• Your hypothesis should be relevant to the research question.

6. What are the 2 types of hypothesis?

• Null hypotheses are used to test the claim that "there is no difference between two groups of data".

• Alternative hypotheses test the claim that "there is a difference between two data groups".

7. Difference between research question and research hypothesis?

A research question is a broad, open-ended question you will try to answer through your research. A hypothesis is a statement based on prior research or theory that you expect to be true due to your study. Example - Research question: What are the factors that influence the adoption of the new technology? Research hypothesis: There is a positive relationship between age, education and income level with the adoption of the new technology.

8. What is plural for hypothesis?

The plural of hypothesis is hypotheses. Here's an example of how it would be used in a statement, "Numerous well-considered hypotheses are presented in this part, and they are supported by tables and figures that are well-illustrated."

9. What is the red queen hypothesis?

The red queen hypothesis in evolutionary biology states that species must constantly evolve to avoid extinction because if they don't, they will be outcompeted by other species that are evolving. Leigh Van Valen first proposed it in 1973; since then, it has been tested and substantiated many times.

10. Who is known as the father of null hypothesis?

The father of the null hypothesis is Sir Ronald Fisher. He published a paper in 1925 that introduced the concept of null hypothesis testing, and he was also the first to use the term itself.

11. When to reject null hypothesis?

You need to find a significant difference between your two populations to reject the null hypothesis. You can determine that by running statistical tests such as an independent sample t-test or a dependent sample t-test. You should reject the null hypothesis if the p-value is less than 0.05.

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Editor-in-Chief for Simply Psychology

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Saul Mcleod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

• A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
• It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
• A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
• Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
• For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
• Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.

Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

• Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.

• Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
• However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

• Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
• Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
• Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
• Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
• Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

• The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
• The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

• Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
• Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
• Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
• Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
• Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
• Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
• Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
• Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

“Theory” vs. “Hypothesis”: What Is The Difference?

Chances are you’ve heard of the TV show The Big Bang Theory . Lots of people love this lighthearted sitcom for its quirky characters and their relationships, but others haven’t even given the series a chance for one reason: they don’t like science and assume the show is boring.

However, it only takes a few seconds with Sheldon and Penny to disprove this assumption and realize that this theory ab0ut The Big Bang Theory is wrong—it isn’t a scientific snoozefest.

But wait: is it a theory or a  hypothesis about the show that leads people astray? And would the actual big bang theory— the one that refers to the beginning of the universe—mean the same thing as a big bang hypothesis ?

Let’s take a closer look at theory and hypothesis to nail down what they mean.

What does theory mean?

As a noun, a theory is a group of tested general propositions “commonly regarded as correct, that can be used as principles of explanation and prediction for a class of phenomena .” This is what is known as a scientific   theory , which by definition is “an understanding that is based on already tested data or results .” Einstein’s theory of relativity and the  theory of evolution are both examples of such tested propositions .

Theory is also defined as a proposed explanation you might make about your own life and observations, and it’s one “whose status is still conjectural and subject to experimentation .” For example:  I’ve got my own theories about why he’s missing his deadlines all the time.  This example refers to an idea that has not yet been proven.

There are other uses of the word theory as well.

• In this example,  theory is “a body of principles or theorems belonging to one subject.” It can be a branch of science or art that deals with its principles or methods .
• For example: when she started to follow a new parenting theory based on a trendy book, it caused a conflict with her mother, who kept offering differing opinions .

First recorded in 1590–1600, theory originates from the Late Latin theōria , which stems from the Greek theōría. Synonyms for theory include approach , assumption , doctrine , ideology , method , philosophy , speculation , thesis , and understanding .

What does hypothesis mean?

Hypothesis is a noun that means “a proposition , or set of propositions, set forth as an explanation” that describe “some specified group of phenomena.” Sounds familiar to theory , no?

But, unlike a theory , a scientific  hypothesis is made before testing is done and isn’t based on results. Instead, it is the basis for further investigation . For example: her working hypothesis is that this new drug also has an unintended effect on the heart, and she is curious what the clinical trials  will show .

Hypothesis also refers to “a proposition assumed as a premise in an argument,” or “mere assumption or guess.” For example:

• She decided to drink more water for a week to test out her hypothesis that dehydration was causing her terrible headaches.
• After a night of her spouse’s maddening snoring, she came up with the hypothesis that sleeping on his back was exacerbating the problem.

Hypothesis was first recorded around 1590–1600 and originates from the Greek word hypóthesis (“basis, supposition”). Synonyms for hypothesis include: assumption , conclusion , conjecture , guess , inference , premise , theorem , and thesis .

How to use each

Although theory in terms of science is used to express something based on extensive research and experimentation, typically in everyday life, theory is used more casually to express an educated guess.

So in casual language,  theory and hypothesis are more likely to be used interchangeably to express an idea or speculation .

In most everyday uses, theory and hypothesis convey the same meaning. For example:

• Her opinion is just a theory , of course. She’s just guessing.
• Her opinion is just a hypothesis , of course. She’s just guessing.

It’s important to remember that a scientific   theory is different. It is based on tested results that support or substantiate it, whereas a hypothesis is formed before the research.

For example:

• His  hypothesis  for the class science project is that this brand of plant food is better than the rest for helping grass grow.
• After testing his hypothesis , he developed a new theory based on the experiment results: plant food B is actually more effective than plant food A in helping grass grow.

In these examples, theory “doesn’t mean a hunch or a guess,” according to Kenneth R. Miller, a cell biologist at Brown University. “A theory is a system of explanations that ties together a whole bunch of facts. It not only explains those facts, but predicts what you ought to find from other observations and experiments.”

So if you have a concept that is based on substantiated research, it’s a theory .

But if you’re working off of an assumption that you still need to test, it’s a hypothesis .

So remember, first comes a hypothesis , then comes theory . Now who’s ready for a  Big Bang Theory marathon?

Now that you’ve theorized and hypothesized through this whole article … keep testing your judgment (Or is it judgement?). Find out the correct spelling here!

Or find out the difference between these two common issues below!

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Hypothesis vs. Theory

What's the difference.

Hypothesis and theory are two terms commonly used in scientific research, but they have distinct meanings and purposes. A hypothesis is a proposed explanation or prediction for a specific phenomenon or observation. It is based on limited evidence and serves as a starting point for further investigation. In contrast, a theory is a well-substantiated explanation that has been extensively tested and supported by a wide range of evidence. It is a comprehensive framework that explains a broad set of phenomena and is widely accepted within the scientific community. While a hypothesis is more tentative and subject to change, a theory represents a higher level of confidence and understanding in the scientific field.

Further Detail

Introduction.

In the realm of scientific inquiry, hypotheses and theories play crucial roles in the development and understanding of knowledge. While both are essential components of the scientific method, they differ in their scope, level of evidence, and the degree of generalization they offer. This article aims to explore and compare the attributes of hypotheses and theories, shedding light on their distinct characteristics and their contributions to scientific progress.

Hypothesis: The Foundation of Scientific Inquiry

A hypothesis is a proposed explanation or prediction for a specific phenomenon or observation. It serves as the initial step in the scientific method, where researchers formulate educated guesses based on existing knowledge and observations. Hypotheses are typically testable and falsifiable, allowing scientists to design experiments or gather data to either support or reject them.

One key attribute of a hypothesis is its specificity. It focuses on a particular aspect of a phenomenon and aims to explain or predict it. For example, a hypothesis might propose that increased exposure to sunlight leads to higher vitamin D levels in humans. This hypothesis is specific to the relationship between sunlight and vitamin D, providing a clear direction for further investigation.

Furthermore, hypotheses are often based on preliminary evidence or observations. They are formulated to address gaps in knowledge or to explain puzzling phenomena. Hypotheses can be derived from existing theories or can emerge from exploratory research. They serve as the foundation for scientific inquiry, guiding researchers towards the collection of empirical evidence.

It is important to note that a hypothesis is not considered a proven fact, even if it is supported by initial evidence. It requires rigorous testing and validation through experimentation and data analysis. If a hypothesis withstands repeated testing and scrutiny, it may evolve into a theory.

Theory: A Comprehensive Explanation

A theory, in the scientific context, is a well-substantiated explanation that encompasses a wide range of observations, experiments, and hypotheses. Unlike a hypothesis, a theory is supported by a substantial body of evidence and has withstood extensive testing and scrutiny. Theories provide a comprehensive framework for understanding natural phenomena and have a higher level of generalization compared to hypotheses.

One key attribute of a theory is its ability to explain and predict a broad range of related phenomena. For example, the theory of evolution by natural selection explains the diversity of life on Earth, the fossil record, and the similarities between different species. Theories are built upon a foundation of multiple lines of evidence, including experimental data, observational studies, and mathematical models.

Theories are also subject to revision and refinement as new evidence emerges. However, this does not undermine their validity or significance. The process of scientific inquiry involves constantly challenging and refining existing theories to accommodate new findings. Theories are not static, but rather dynamic frameworks that adapt to incorporate new knowledge.

Moreover, theories are widely accepted within the scientific community due to their robustness and explanatory power. They have undergone rigorous peer review and scrutiny, ensuring that they meet the highest standards of scientific integrity. Theories provide a solid foundation for further research and serve as a basis for the development of new hypotheses.

Comparing Hypotheses and Theories

While hypotheses and theories share the common goal of explaining natural phenomena, they differ in several key attributes. Let's explore some of the main points of comparison:

Scope and Generalization

Hypotheses are typically narrow in scope, focusing on specific aspects of a phenomenon. They aim to explain or predict a particular observation or relationship. In contrast, theories have a broader scope and offer a higher level of generalization. They provide comprehensive explanations that encompass multiple phenomena and observations.

Evidence and Testing

Hypotheses are formulated based on preliminary evidence or observations. They serve as starting points for scientific investigation and require empirical testing to determine their validity. Hypotheses are often tested through experiments, data analysis, or observational studies. Theories, on the other hand, are supported by a substantial body of evidence. They have withstood extensive testing and scrutiny, incorporating multiple lines of evidence from various sources.

Level of Certainty

Due to their preliminary nature, hypotheses do not offer a high level of certainty. They are educated guesses that require further testing and validation. In contrast, theories provide a higher level of certainty and confidence. They are well-substantiated explanations that have been extensively tested and supported by a wide range of evidence.

Development and Evolution

Hypotheses are often derived from existing theories or emerge from exploratory research. They serve as the initial step in scientific inquiry and can evolve into theories if supported by substantial evidence. Theories, on the other hand, are the result of extensive research, testing, and refinement. They are dynamic frameworks that adapt to incorporate new evidence and insights.

Acceptance and Consensus

While hypotheses are subject to individual interpretation and may vary among researchers, theories are widely accepted within the scientific community. Theories have undergone rigorous peer review and scrutiny, ensuring a high level of consensus among experts. They provide a solid foundation for scientific understanding and guide further research.

In summary, hypotheses and theories are integral components of the scientific method, each serving distinct roles in the pursuit of knowledge. Hypotheses provide specific explanations or predictions for phenomena and act as the initial step in scientific inquiry. They require empirical testing and validation to determine their validity. Theories, on the other hand, offer comprehensive explanations that encompass a wide range of observations and have withstood extensive testing. They provide a higher level of generalization and serve as the foundation for scientific understanding. While hypotheses and theories differ in their scope, level of evidence, and generalization, they both contribute to the advancement of scientific knowledge and our understanding of the natural world.

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How to Write a Great Hypothesis

Hypothesis Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis, operational definitions, types of hypotheses, hypotheses examples.

• Collecting Data

Frequently Asked Questions

A hypothesis is a tentative statement about the relationship between two or more  variables. It is a specific, testable prediction about what you expect to happen in a study.

One hypothesis example would be a study designed to look at the relationship between sleep deprivation and test performance might have a hypothesis that states: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. It is only at this point that researchers begin to develop a testable hypothesis. Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore a number of factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk wisdom that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis.   In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in a number of different ways. One of the basic principles of any type of scientific research is that the results must be replicable.   By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. How would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

In order to measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming other people. In this situation, the researcher might utilize a simulated task to measure aggressiveness.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests that there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type of hypothesis suggests a relationship between three or more variables, such as two independent variables and a dependent variable.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative sample of the population and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• Complex hypothesis: "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "Children who receive a new reading intervention will have scores different than students who do not receive the intervention."
• "There will be no difference in scores on a memory recall task between children and adults."

Examples of an alternative hypothesis:

• "Children who receive a new reading intervention will perform better than students who did not receive the intervention."
• "Adults will perform better on a memory task than children." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when it would be impossible or difficult to  conduct an experiment . These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a correlational study can then be used to look at how the variables are related. This type of research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

A Word From Verywell

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Some examples of how to write a hypothesis include:

• "Staying up late will lead to worse test performance the next day."
• "People who consume one apple each day will visit the doctor fewer times each year."
• "Breaking study sessions up into three 20-minute sessions will lead to better test results than a single 60-minute study session."

The four parts of a hypothesis are:

• The research question
• The independent variable (IV)
• The dependent variable (DV)
• The proposed relationship between the IV and DV

Castillo M. The scientific method: a need for something better? . AJNR Am J Neuroradiol. 2013;34(9):1669-71. doi:10.3174/ajnr.A3401

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

• Key Differences

Know the Differences & Comparisons

Difference Between Hypothesis and Theory

The term ‘hypothesis’ is often contrasted with the term theory which implies an idea, typically proven, which aims at explaining facts and events. Both hypothesis and theory are important components of developing an approach, but these are not same. There exist a fine line of difference between hypothesis and theory, discussed in this article, have a look.

Content: Hypothesis Vs Theory

Comparison chart, definition of hypothesis.

An unproven statement or a mere assumption to be proved or disproved, about a factor, on which the researcher is interested, is called a hypothesis. It is a tentative statement, which is concerned with the relationship between two or more phenomena, as specified by the theoretical framework. The hypothesis has to go through a test, to determine its validity.

In other words, the hypothesis is a predictive statement, which can be objectively verified and tested through scientific methods, and relates the independent factor to the dependent one. To a researcher, a hypothesis is more like a question which he intends to resolve. The salient features of hypothesis are:

• It must be clear and precise or else the reliability of the inferences drawn will be questioned.
• It can be put to the test.
• If the hypothesis is relational, it should state the relationship between independent and dependent variables.
• The hypothesis should be open and responsive to testing within the stipulated time.
• It should be limited in scope and must be clearly defined.

Definition of Theory

An idea or a broad range of ideas that are assumed to be true, which aims at explaining cause and effect relationship between multiple observed phenomena. It is based on hypothesis, which after a thorough analysis and continuous testing and confirmation through observation and experiments, becomes a theory. As it is backed by evidence, it is scientifically proven.

Just like hypothesis, theories can also be accepted or rejected. As more and more information is gathered on the subject, theories are modified accordingly, to increase the accuracy of prediction over time.

Key Differences Between Hypothesis and Theory

The points given below are vital, so far as the difference between hypothesis and theory is concerned:

• Hypothesis refers to a supposition, based on few pieces of evidence, as an inception of further research or investigation. A theory is a well-affirmed explanation of natural phenomena, which is frequently validated through experimentation and observation.
• While the hypothesis is based on a little amount of data, the theory is based on a wide set of data.
• The hypothesis is an unproven statement; that can be tested. On the other hand, the theory is a scientifically tested and proven explanation of fact or event.
• Hypothesis relies on suggestions, prediction, possibility or projects whereas a theory is supported by evidence and is verified.
• The hypothesis may or may not be proved true, so the result is uncertain. On the contrary, the theory is one, that is assumed to be true and so its result is certain.
• Hypothesis and theory are two levels of the scientific method, i.e. theory follows hypothesis and the basis for research is hypothesis whose outcome is a theory.

Both hypothesis and theory are testable and falsifiable. When a hypothesis is proved true, by passing all critical tests and analysis, it becomes a theory. So, the hypothesis is very different from theory, as the former is something unproven but the latter is a proven and tested statement.

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BELLENS MOTEBEJANE says

July 15, 2019 at 2:31 pm

AMAIZING !WHAT ARE THE DIFFERENCE BETWEEN THEORY AND LAW?

February 17, 2022 at 3:47 am

Thanks, I’m finally clear on this for the first time in my life of 65 years

Curtis Le Gendre says

September 14, 2022 at 8:02 am

Great Information

Kenneth says

November 19, 2022 at 2:10 am

I was looking for some takes on this topic, and I found your article quite informative. It has given me a fresh perspective on the topic tackled. Thanks!

Stefanie Banis says

February 9, 2024 at 6:35 pm

Very informative! Thank you! I understand the difference much better now!

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Methodology

• How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

• An independent variable is something the researcher changes or controls.
• A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

• H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
• H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

• Sampling methods
• Simple random sampling
• Stratified sampling
• Cluster sampling
• Likert scales
• Reproducibility

 Statistics

• Null hypothesis
• Statistical power
• Probability distribution
• Effect size
• Poisson distribution

Research bias

• Optimism bias
• Cognitive bias
• Implicit bias
• Hawthorne effect
• Anchoring bias
• Explicit bias

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A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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McCombes, S. (2023, November 20). How to Write a Strong Hypothesis | Steps & Examples. Scribbr. Retrieved April 15, 2024, from https://www.scribbr.com/methodology/hypothesis/

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• Biology Difference Between
• Difference Between Hypothesis And Theory

Difference Between Theory and Hypothesis

Many of them belittle evolution because “it is just a theory.” Gravity, on the other hand, must be real because it is a law. The words “theory,” “facts,” “laws” and “hypothesis” have a very specific meaning in the scientific world that doesn’t quite match the ones we use in everyday language. A hypothesis is a tentative explanation of an observation that can be tested. It acts as a starting point for further explanation. Theory, on the other hand, is an explanation of some aspect of the natural world that’s well-justified by facts, tested hypotheses, and laws. Let us look at more differences between hypothesis and theory given in a tabular column below.

Theory vs Hypothesis

From the above differences, we can infer that a hypothesis might change significantly as the testing occurs. A hypothesis can either be right or wrong. When a hypothesis is tested and proved true, it becomes a theory. At BYJU’S, learn more differences like the difference between asteroid and comet.

Put your understanding of this concept to test by answering a few MCQs. Click ‘Start Quiz’ to begin!

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Thesis vs Hypothesis vs Theory: the Differences and examples

thesis hypothesis and theory

Many students may have a hard time understanding the differences between a thesis, a hypothesis, and a theory. It is important to understand their differences. Such an understanding will be instrumental.

More so, when writing complex research papers that require a thesis that has a hypothesis and utilizes theories. We have gathered from responses of our college writing service that the difference between the three is confusing.

That being said, this article is meant to explain the differences between a thesis, a hypothesis, and a theory. 

Difference between Hypothesis and Thesis

There are major differences between hypothesis and thesis. While they seem to be related on the face, their differences are huge both in concept and practice.

A hypothesis is a proposed explanation of something or a phenomenon. A scientific hypothesis uses a scientific method that requires any hypothesis to be tested. As such, scientists and researchers base their hypothesis on observations that have been previously made and that which cannot be explained by the available or prevailing scientific theories.

From the definition of a hypothesis, you can see that theories must be included in any scientific method. This is the reason why this article tries to differentiate a thesis, a hypothesis, and a theory. 

Moving forward, a thesis can be defined as a written piece of academic work that is submitted by students to attain a university degree. However, on a smaller scale, there is something that is referred to as a thesis statement.

This is written at the introduction of a research paper or essay that is supported by a credible argument. The link between a hypothesis and thesis is that a thesis is a distinction or an affirmation of the hypothesis.

What this means is that whenever a research paper contains a hypothesis, there should be a thesis that validates it. 

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What is a Hypothesis?

A hypothesis can be defined as the proposed or suggested explanation for an occurrence, something, or a phenomenon. It should be testable through scientific methods. The reason why scholarly works should have a hypothesis is that the observed phenomena could not be explained using the prevailing scientific theories hence the reason why it should be tested. 

Testing the hypothesis may result in the development of new or improved scientific theories that are beneficial to the discipline and society in general. 

What is a Thesis?

A thesis is a written piece of academic work that is submitted by students to attain a university degree. When a thesis is used as a stand-alone word, it denotes academic papers written by university students. It is mostly written by those pursuing postgraduate degrees, at the end of their courses. They demonstrate their proficiency in their disciplines and the topics they have selected for research. 

However, when a thesis is used to refer to a statement, it denotes the statement that is written at the introduction of a research paper or essay. A thesis is supported by a credible argument.

Every research paper must have a thesis statement that acts as a guide to what the research will be all about. It is possible to receive very poor grades or even score a zero if your research paper lacks the thesis statement. 

What is a Theory?

A theory can be defined as a rational form of abstract perspectives or thinking concerning the results of such thinking or a phenomenon. The process of rational and contemplative thinking is mostly associated with processes such as research or observational study.

As such, a theory can be considered to belong to both scientific and non-scientific disciplines. Theories can also belong to no discipline.

From a modernistic scientific approach, a theory can mean scientific theories that have been well confirmed to explain nature and that are created in such a way that they are consistent with the standard scientific method. A theory should fulfill all the criteria required by modern-day science. 

A theory should be described in a way that scientific tests that have been conducted can provide empirical support or contradiction to the theory.

Because of the nature by which scientific theories are developed, they tend to be the most rigorous, reliable, and comprehensive when it comes to describing and supporting scientific knowledge. 

The connection between a theory and a hypothesis is that when a theory has not yet been proven, it can be referred to as a hypothesis.

The thing about theories is that they are not meant to help the scientist or researcher reach a particular goal. Rather, a theory is meant to guide the process of finding facts about a phenomenon or an observation. 

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Difference between a Theory and Thesis

A theory is a rational form of abstract perspectives or thinking concerning the results of such thinking or a phenomenon. The process of rational and contemplative thinking is mostly associated with processes such as research or observational study. On the other hand, a thesis is a written piece of academic work that is submitted by students to attain a university degree.

It denotes academic papers that are written by students in the university, especially those pursuing postgraduate degrees, at the end of their courses to demonstrate their proficiency in their disciplines and the topics they have selected for research. 

To understand the application of these, read our guide on the difference between a research paper and a thesis proposal to get a wider view.

How to write a Good Hypothesis

1. asking a question.

Asking a question is the first step in the scientific method and the question should be based on  who, what, where, when, why,  and  how . The question should be focused, specific, and researchable.

2. Gathering preliminary research 

This is the process of collecting relevant data. It can be done by researching academic journals, conducting case studies, observing phenomena, and conducting experiments. 

3. Formulating an answer

When the research is completed, you should think of how best to answer the question and defend your position. The answer to your question should be objective. 

4. Writing the hypothesis

When your answer is ready, you can move to the next step of formulating the hypothesis. A good hypothesis should contain relevant variables, predicted outcomes, and a study group that can include non-human things. The hypothesis should not be a question but a complete statement. 

5. Refining the hypothesis

Though you may skip this step, it is advisable to include it because your study may involve two groups or be a correlational study. Refining the hypothesis will ensure that you have stated the difference or relationship you expect to find. 

6. Creating a null and alternative hypotheses

A null hypothesis (H0) will postulate that there is no evidence to support the difference. On the other hand, an alternative hypothesis (H1) posits that there is evidence in support of the difference. 

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Frequently Asked Questions

Difference between thesis and hypothesis example.

Thesis:  High levels of alcohol consumption have detrimental effects on your health, such as weight gain, heart disease, and liver complications.

Hypothesis:  The people who consume high levels of alcohol experience detrimental effects on their health such as weight gain, heart disease, and liver complications. 

What is the difference between a summary and a thesis statement?

A summary is a brief account or statement of the main points from the researches. A thesis statement is a statement that is written at the end of the introduction of a research paper or essay that summarizes the main claims of the paper. 

Difference between hypothesis and statement of the problem

A hypothesis can be defined as the proposed or suggested explanation for an occurrence, something, or a phenomenon. The same should be testable through scientific methods. Conversely, a statement of a problem is a concise description of the issue to be addressed on how it can be improved. 

When not handling complex essays and academic writing tasks, Josh is busy advising students on how to pass assignments. In spare time, he loves playing football or walking with his dog around the park.

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Conjecture and hypothesis: The importance of reality checks

David deamer.

1 Department of Biomolecular Engineering, University of California, Santa Cruz CA 95060, USA

In origins of life research, it is important to understand the difference between conjecture and hypothesis. This commentary explores the difference and recommends alternative hypotheses as a way to advance our understanding of how life can begin on the Earth and other habitable planets. As an example of how this approach can be used, two conditions have been proposed for sites conducive to the origin of life: hydrothermal vents in salty seawater, and fresh water hydrothermal fields associated with volcanic landmasses. These are considered as alternative hypotheses and the accumulating weight of evidence for each site is described and analyzed.

Introduction

The word conjecture is defined as an opinion based on incomplete information. The word can be taken to be slightly pejorative, but given that conjecture also involves imagination and creative effort, I will argue here that in scientific research there is a natural progression from conjecture to hypothesis to consensus. Conjecture is an idea, hypothesis is a conjecture that can be tested by experiment or observation, and consensus emerges when other interested colleagues agree that evidence supports a hypothesis that has explanatory value. This approach is clearly relevant to origins of life research which is still at a stage where multiple conjectures abound yet vast gaps in knowledge and understanding remain, mostly due to lack of significant funding for research in this area. The result is that only a few dozen laboratories are supported in the global scientific community, in contrast to thousands of scientists investigating health related research or chemistry and physics having applications in industry. Another reason is that the origin of life is best understood in interdisciplinary terms involving knowledge of astronomy, planetary science, biophysics, chemistry and biochemistry, molecular biology and evolution. Relatively few scientists have a taste for research that demands such broad knowledge to make significant advances. The historical development of origins research has been well described by Iris Fry [ 1 ] and Antonio Lazcano [ 2 ].

Most scientists agree that hypothesis testing is an essential feature of research, and a typical proposal to a funding agency usually has a clearly stated hypothesis. However, there is a very human tendency for investigators to prefer positive results that support their idea. Karl Popper [ 3 ] had some good advice in this regard: Don't try to prove an idea is right. Instead, try to falsify it. Those rare ideas that cannot be falsified then emerge from the majority of ideas that fail the testing process. Günther Wächtershäuser [ 4 ] recently commented on how Popper's advice can be applied in origins of life research.

Hypothesis testing is an essential feature of good research, but its value can be increased by one additional step which was first clearly stated in 1964 by John Platt [ 5 ]. The title of Platt's article was Strong Inference , which he defines in the following way:

“Strong inference consists of applying the following steps to every problem in science, formally and explicitly and regularly.

• Devising alternative hypotheses.
• Devising crucial experiments ... with alternative possible outcomes, each of which will, as nearly as possible, exclude one or more of the hypotheses.
• Carrying out the experiment so as to get a clean result.”

Research approaches that incorporate alternative hypotheses avoid the tendency to prefer positive results, because both positive and negative results have value in inferring which of the two alternatives is better supported by accumulating evidence. The aim of this commentary is to describe how alternative hypotheses can be applied to understanding the origin of life, with the focus on a simple question: Did life begin in salty water in a marine environment, or did life begin in fresh water in a terrestrial setting? Although the question seems simple, there are significant ramifications of possible answers for life detection missions to other planetary objects in the solar system.

We can begin with two conjectures and then attempt to turn them into alternative hypotheses. The first conjecture follows from the discovery of hydrothermal vents and observations related to their properties:

• All life requires liquid water
• Most of the water on Earth is in the ocean.
• Hydrothermal vents emerging from the ocean floor are sources of chemical energy.
• Populations of chemotrophic microbial life thrive in hydrothermal vents.

Conjecture: life originated in hydrothermal vents and later adapted to fresh water on volcanic and continental land masses. In the absence of alternatives this idea has been accepted as a reasonable suggestion.

Is there an alternative? Here is another list of facts:

• A small fraction of the Earth's water is distilled from seawater and precipitates as fresh water on volcanic land masses.
• The water accumulates in hydrothermal fields that undergo cycles of evaporation and refilling.
• During evaporation, dilute solutes in the water become concentrated films on mineral surfaces.
• If the solutes can undergo chemical or physical interactions, they will do so in the concentrated films.
• The products will accumulate in the pools when water returns either in the form of precipitation or as fluctuations in water levels related to hot springs or geyser activity.

Conjecture: life originated in fresh water hydrothermal fields associated with volcanic land masses, then adapted to the salty seawater of the early ocean.

The current paradigm: Life began in the ocean in salty seawater

Now we can provide a few more details about two geophysical conditions that have been proposed as alternative sites conducive for the origin of life. Hydrothermal vents were discovered in 1977 [ 6 ] and were soon proposed to be a likely site for life to begin [ 7 – 10 ]. Hydrothermal vents referred to as black smokers are produced when seawater comes into contact with rocks heated by magma underlying mid-ocean ridges. The hot water dissolves mineral components of the rock and then emerges through the ocean floor where the mineral solutes come out of solution to form characteristic chimneys that emit a black smoke of precipitated metal sulfide particles.

A second type of hydrothermal vent was discovered in 2001 [ 11 ] that does not depend on volcanism. Instead they form when seawater reacts with mineral components of peridotite in the sea floor, a process called serpentinization. The reaction produces hydrogen and a strongly alkaline (pH 9–11) hot medium saturated with carbonate. When the warm fluid contacts cooler seawater, calcium carbonate and other minerals precipitate to form white chimney structures.

The hydrogen gas dissolved in the alkaline vent fluid is a potential source of reducing power. Certain microorganisms already use hydrogen for this purpose, so the hydrothermal vent hypothesis proposes that on the prebiotic Earth hydrogen could potentially reduce carbon dioxide to organic compounds that are then incorporated into a primitive metabolism [ 12 ]. Lane and Martin [ 13 ] noted that the alkaline vent minerals have a porous structure that could serve as cellular compartments with mineral membranes as boundaries. The assumption that the membranes may separate a strongly alkaline medium from mildly acidic Hadean sea water across suggested that a primitive version of chemiosmotic energy transduction might be possible to supply chemical energy for primitive forms of life. Weiss et al. [ 14 ] used genomic analysis of vent microorganisms to test the possibility that the last universal common ancestor (LUCA) may have originated in hydrothermal vents.

The iron-sulfur chemistry proposed for hydrothermal vents was tested by Huber and Wächtershäuser [ 15 – 16 ] who simulated vent conditions with boiling mixtures of iron and nickel sulfides to which various reactants were added. They reported that acetic acid, amino acids and peptide bonds could be synthesized under these conditions, and claimed that “The results support the theory of a chemoautotrophic origin of life with a CO-driven, (Fe,Ni)S-dependent primordial metabolism.”

More recently Herschy et al. [ 17 ] simulated hydrothermal vent conditions by injecting a solution of potassium phosphate, sodium silicate and sodium sulfide (pH 11) into a second solution of ferrous chloride, sodium bicarbonate and nickel chloride (pH 5). The aim was to determine whether carbon dioxide (present as 10 mM sodium bicarbonate) can be reduced under these conditions, and they were able to detect ≈50 μM formic acid. In a similar laboratory simulation of an alkaline hydrothermal vent, Burcar et al. [ 18 ] used mass spectrometry to detect a small yield of dimers produced from adenosine monophosphate circulating in the medium.

An alternative hypothesis: Life began in terrestrial fresh water

Although most of the Earth's water today is salty seawater, a small fraction (~1%) is present in the form of fresh water distilled by evaporation from the ocean and falling on continental land masses as precipitation. The Hadean Earth did not have continents but was likely to have volcanoes similar to those from the same era still visible on Mars. The volcanism associated with such islands suggests an alternative hydrothermal site we will refer to as hydrothermal fields. Iceland is an analogous site on today's Earth, with several active volcanoes and associated hydrothermal areas supplied by precipitation and dominated by hot springs and geyser activity. In contrast to the single rock-water interface of hydrothermal vents, hydrothermal fields have a more complex array of three interfaces in which minerals, water and atmosphere undergo continuous fluctuations of wetting and drying.

The fluctuating hydrothermal field hypothesis has been used as a model for polymerization reactions in which monomers like amino acids and mononucleotides form peptide and ester bonds of biologically relevant polymers. The idea that evaporation and heat can drive polymerization is obvious and was first proposed years ago [ 19 ]. Lahav and White [ 20 ] adopted the approach and demonstrated that peptide bonds could be produced using clay as a catalyst. The approach was largely abandoned with the advent of the RNA World scenario that suggested a way for life to begin in solution, rather than by evaporation to dryness. However, polymerization in an aqueous medium requires chemical activation of the monomers, and so far there is no obvious mechanism by which activation can occur. Recent studies have returned to evaporation as a way to drive polymerization reactions [ 21 – 22 ].

There are several advantages to using evaporation in this regard. First, simply concentrating potential reactants adds significant free energy to a system that can be used to drive condensation reactions [ 23 ]. Furthermore, if amphiphilic compounds are present they can organize and concentrate reactants within a two dimensional plane with the result that polymerization is enhanced [ 24 – 25 ].

The hydrothermal field hypothesis has been tested in laboratory simulations. For instance, peptide bonds have been produced [ 26 – 27 ] and cycles of drying and rehydration have been shown to drive polymerization of mononucleotides [ 22 , 28 – 29 ]. Because the resulting polymers can be encapsulated in lipid vesicles, it has been proposed that the resulting protocells are candidates for combinatorial selection and the first steps of evolution [ 30 ].

From the above discussion, alternative conjectures have been published and are available for critical analysis and commentary. How can we turn the two conjectures into John Platt's alternative hypotheses? The answer is simple. We follow Platt's advice to devise critical experiments that will add weight of evidence to either or both of the alternative conjectures which then become testable hypotheses. Here is a proposed list of conditions that seem to be essential prerequisites if cellular life is to originate in one of the two alternative conditions:

• There must be a source of organic compounds relevant to biological processes, such as amino acids, nucleobases, simple sugars and phosphate.
• The organic solutes are likely to be present as very dilute solutions, so there should be a process by which they can be sufficiently concentrated to undergo chemical reactions relevant to cellular life.
• Energy sources must be present in the environment to drive a primitive metabolism and polymerization.
• Products of reactions should accumulate within the site rather than dispersing into the bulk phase environment.
• Biologically relevant polymers are synthesized with chain lengths sufficient to act as catalysts or incorporate genetic information.
• If amphiphilic compounds are present in the mixture, the conditions will allow them to assemble into membranous compartments.
• A plausible physical mechanism can produce encapsulated polymers in the form of protocells and subject them to combinatorial selection.

These conditions can also be considered to be predictions, because each condition in the above list can be tested by observation, by theoretical analysis or in laboratory simulations. If any one of the predictions fails experimentally or is shown to be impossible, for instance by being inconsistent with thermodynamic principles, that alternative can be considered to be falsified. As evidence accumulates, we will be able to judge the relative plausibility and explanatory power of the competing ideas. Continued testing of the alternative hypotheses is essential, because neither has yet reached the level of consensus. In both cases, laboratory simulations will ideally be extended to a second important step, which is to visit the alternative sites and demonstrate that what happens in the laboratory can also occur in the actual conditions of hydrothermal vents or fields.

This article is part of the Thematic Series "From prebiotic chemistry to molecular evolution".